Hydrocortisone에 依하여 Inducek된 Serine Dehydratase의 代謝?果

趙武衍,金濟炫 中央醫學社 1975 中央醫學 Vol.29 No.2

Hepatic serine dehydratase was induced with 5 mg of hydrocortisone by intraper-itoneal administration in albino rats weighing around 200 gm. Serine metabolism was observed in intact and in the isolated perfused liver. The results were as follows: 1) Serine dehydratase activity was two times higher in the hydocortisone treated group than in the controls in intact and in the isolated perfuse liver. 2) In vivo experiments blood concentrations of pyruvate was lower in the hydrocortisone group than in the control, whereas respiratory14CO2 was no dif?ference between hydrocortisone and control groups. 3) In the isolated perfuse liver: i) net cumulative production of pyruvate in the hydrocortisone group was less than that of the control in group after 200mg and 1. 2gm administrations of L-serine to each. group. ii) net cumulative production of glucose after 200mg administration was lower in the hydrocortisone group than that of the control. This lower value was maintained until the end of the perfusion. After administration of 1. 2gm serine glucose production was continuously raised but no difference between the hydrocortisone and control groups was observed. iii) liver glycogen content in the hydrocortisone group was higher than that of the control but there is no difference after administration of different dosages of L-serine. From the above results it may be concluded that induced serine dehydratase activity was not parallel to the production of pyruvate, glucose, respiratory CO2 and liver glycogen.

Cruciate Paralysis를 동반한 치상돌기 골절에 의한 제1경추골 탈구

조무연,신종하,최천식,주문배 대한신경외과학회 1991 Journal of Korean neurosurgical society Vol.20 No.7

Cruciate paralysis, an unusual clinical entity, is "frequently undiagnosed or misunderstood", and can simulate the acute cervical central-cord injury syndrome. This rare injury pattern is characterized by weakness of upper extremities with little of no compromise of lower limb function following trauma to the superior cervical cord. The patterns of injury and clinical findings support selective damage to the corticospinal tract or upper motor neurons subserving upper limb function with the pyramidal decussation. Authors experience a case of cruciate paralysis due to type 2 odontoid fracture and atlas dislocation.

CHROMSOMAL SUBFRACTION에서BENZO(A)PYRENE화합물의 생화학적 연구

조무연,이송재,정인철,정인권,노준 高神大學 醫學部 1990 高神大學醫學部 論文集 Vol.6 No.2

배양 뼈에 있어서 Calcium 대사에 대한 Hydrocortisone 의 효과

조무연,송정석 생화학분자생물학회 1978 BMB Reports Vol.6 No.3

Serine Dehydratase 의 Hormonal Induction 의 생리적 의의에 대한 실험적 연구

조무연,김제현 생화학분자생물학회 1981 BMB Reports Vol.8 No.1

Dehydro - L - ascorbic Acid 가 백서 간에서 Vitamin A 대사에 미치는 영향

조무연,김현옥,송정석 ( Moo Youn Cho,Hyun Ock Kim,Chung Suk Song ) 생화학분자생물학회 1976 BMB Reports Vol.9 No.2

The effect of dehydro-L-ascorbic acid (DHA) on the transfer of vitamin A to the liver was observed in the isolated rat liver perfusion. DHA had no effect on the transfer of vitamin A to the liver. The addition of DHA to the perfusate did not raise the level of either oxidized or reduced form of vitamin A in the liver. From this fact, DHA seems to be destroyed as soon as it is absorbed into the liver or not to be absorbed to the liver and rather to be destroyed in the blood.

식이조성을 달리한 백서에 만성 알콜투여가 간장조직 내 malic enzyme 및 ATP-citrate lyase활성에 미치는 실험적 연구

조무연,김윤수,Cho, Moo-Youn,Kim, Yoon-Soo 생화학분자생물학회 1978 한국생화학회지 Vol.11 No.1

간장조직 내 lipogenic enzyme인 malic enzyme과 ATP-citrate lyase의 활성이 식이조성을 달리한 사료로 사육한 백서에서 어떻게 영향을 받으며 이동안 만성알콜투여 (6.7Cal/day)로 오는 효소활성 및 간장조직 내 지방 형성과 어떠한 상관 관계를 가지는가 관찰한 바 식이조성 차이로 오는 효소 활성은 고함수탄소 식이군에서 malic enzyme 활성만이 높았고 고지방식이군, 저함수탄소 식이군에서는 정상식이군에 비해 감소하고 알콜투여에 의한 영향은 볼 수 없었다. 간장조직내 식이에 의한 triglyceride 함량의 변화는 정상식이군에 비해 고지방식이군, 저함수탄소식이군에서는 높으나 알콜투여에 의한 효과는 고지방식이군에서만 현저한 증가를 나타냈다. 혈청내 유리지방산 농도는 알콜투여에 의해 정상식이군에서는 1.5배(倍), 저함수탄소식이군에서는 3배(倍)의 증가가 있었으며 고지방식이군에서는 차이가 없으며 고함수탄소식이군에서는 알콜투여군이 대조군에 비해 오히려 낮았다. 식이 조성및 알콜투여에 의한 혈청내 Phospholipid 함량에는 변화가 없었다. Influences of diet composition on the activities of two lipogenic enzymes, malic enzyme and ATP-citrate lyase, and on the hepatic triglyceride accumlation were investigated in rats. In addition, the effect of chronic administration of ethanol on these enzymes and hepatic triglyceride accumulation were studied. The results were summarized as fallowing. 1) The activity of malic enzyme in the liver of rat fed high-fat (HF), low-carbohydrate (LC) and high-carbohydrate (HC) diet groups was 62%, 55% and 155% of that of the normal level, and the ATP-citrate lyase activity was 22%, 31% and 44% of that of normal level, respectively. These changes of the lipogenic enzyme activities were not altered by chronic administration of ethanol to the rats. 2) Average content of triglyceride in the liver of rat in normal diet group was 9.27mg per g. of liver, 16.37mg in the normal diet plus ethanol treated group, 37.79mg in the HF diet group, 75.7mg in the HF plus ethanol treated group, 14.78mg in the LC diet group, 17.87mg in the LC diet plus ethanol treated group, 11.61mg in the HC group, 14.92mg in the LC diet plus ethanol treated group. These results, shows that the administration of ethanol was increased hepatic triglyceride content only in the high fat diet group. 3) Free fatty acid concentration was increased in the serum of rats free fatty acid concentration was decreased by 40% in the high carbohydrate group, may be due to the increased or decreased mobilization of lipid from peripheral adipose tissue. 4) No total phospholipid content in the serum of rat was observed among above different diet and plus alcohol treated group. It is therefore apparent that ethanol administration induces the hepatic accumulation of triglyceride in the HF diet group, whereas it reduces the hepatic accumulation of triglyceride and serum free fatty acid level in the HC diet group.

Genomic changes of c-myc, c-H-ras in benzo(a)pyrene and dimethylbenz(a)anthracene treated human lymphoblast NC-37 cells

조무연,어완규,이상욱,정인철,Cho, Moo Youn,Eo, Wan Kyu,Lee, Sang Uk,Jeong, In cheol Korean Society of Life Science 1995 생명과학회지 Vol.5 No.3

To investigate genomic changes in c-myc gene by a chemical carcinogen, human lymphoblast NC-37 cells were exposed to benzo(a)pyrene(BP) and dimethylbenzanthracene(DMBA), and the c-myc gene expression was evaluated by Northern and Southern blot hybridization techniques. The results are as follows: When the genomic DNA of NC-37 cells exposed to several concentrations(1.25, 2.5 and 5ug/ml) of BP concentration. However, the c-myc gene was most significantly enhanced with 2.5ug/ml of BP. The expressions of c-myc gene in NC-37 cells was stimulated by BP and DMBA. Addition of TPA reduced the gene expression BP-treated cells, whereas it enhanced the gene expression in DMBA-treated cells. The expression of c-H-ras gene was slightly increased by treatment with BP and DMBA alone and in combination with TPA, however the magnitude of increase was not significantly different between each other. The expressions of c-myc c-H-ras genes in Burkitt's lymphoma cells were greater than those in NC-37 cells. When the DNA extracted from NC-37 cells exposed to various concentrations of BP were amplified by polymerase chain reaction using a primer set containing c-myc exon I, the amplified products were of the same size in all groups. To evaluate the BP toxicity in E.coli to which human c-myc gene-cloned pBR322 vector was inserted, Southern blot hybridization was conducted on c-myc genes digested with EcoRI/HindIII and Smal/Xbal restriction enzymes, and observing that in 2 ug/ml BP-treated cells a 3.5kb fragment was generated in addition to 1.3kb fragment which can be observed in normal cells. Direct nucleotide sequence analysis of polymerase chain reaction products showed a mutation of G$\longrightarrow$A transition at the Smal recognition site.

백서에서 L - Tryptophan 의 과량 투여시 간장내 Triglyceride 축적에 미치는 호르몬의 효과

조무연,김윤수 ( Moo Youn Cho,Yoon Soo Kim ) 생화학분자생물학회 1978 BMB Reports Vol.11 No.1

When an excess amount of L-tryptophan (0.34mmole) was administered intraperitioneally to a normal rat the triglyceride content in the liver increased two times that of the normal control in three hours, but no significant changes of triglyceride content in the liver of adrenalectomized rat was noted, however, the triglyceride content in liver and FFA in the plasma of adrenalectomized rat administered epinephrine and L-tryptophan together was increased as much as one and a half times than that of the control adrenalectomized rat which received L-tryptophan or epinephrine only. No effects of hydrocortisone, glucagon and insulin on the triglyceride accumulation in the liver were noted in adrenalectomized rat receiving each hormone or L-tryptophan only, or hormone and L-tryptophan together. Therefore, it was confirmed that the triglyceride accumulation in the liver induced by L-tryptophan is due to the indirect effect of L-tryptophan through stimulation of epinephrine secretion.

식이조성을 달리한 백서에 만성 알콜투여가 간장조직내 malic enzyme 및 ATP - citrate lyase 활성에 미치는 실험적 연구

조무연,김윤수 ( Moo Youn Cho,Yoon Soo Kim ) 생화학분자생물학회 1978 BMB Reports Vol.11 No.1

Influences of diet composition on the activities of two lipogenic enzymes, malic enzyme and ATP-citrate lyase, and on the hepatic triglyceride accumlation were investigated in rats. In addition, the effect of chronic administration of ethanol on these enzymes and hepatic triglyceride accumulation were studied. The results were summarized as following. 1) The activity of malic enzyme in the liver of rat fed hibh-fat (HF), low-carbohydrate (LC) and high-carbohydrate (HC) diet groups was 62%, 55% and 155% of that of the normal level, and the ATP-citrate lyase activity was 22%, 31% and 44% of that of normal level, respectively. These changes of the lipogenic enzyme activities were not altered by chronic administration of ethanol to the rats. 2) Average content of triglyceride in the liver of rat in normal diet group was 9.27㎎ per g. of liver, 16.37㎎ in the normal diet plus ethanol treated group, 37.79㎎ in the HF diet group, 75.7㎎ in the HF plus ethanol treai:ed group, 14.78㎎ in the LC diet group, 17.87㎎ in the LC diet plus ethanol treated group, 11.61㎎ in the HC group, 14.92㎎ in the LC diet plus ethanol treated group. These results, shows that the administration of ethanol was increased hepatic triglyceride content only in the high fat diet group. 3) Free fatty acid concentration was increased in the serum of rats free fatty acid concentration was decreased by 40% in the high carbohydrate group, may be due to the increased or decreased mobilization of lipid from peripheral adipose tissue. 4) No total phospholipid content in the serum of rat was observed among above different diet and plus alcohol treated group. It is therefore apparent that ethanol administration induces the hepatic accumulation of triglyceride in the HF diet group, whereas it reduces the hepatic accumulation of triglyceride and serum free fatty acid level in the HC diet group.