염증성 장질환

홍경란 약업신문사 2001 의약정보 Vol.2001 No.7

사례로 배우는 당뇨병 환자의 복약상담

홍경란 韓國病院藥師會 2007 병원약사회지 Vol.24 No.3

강남성모병원 퇴원환자 복약상담 업무 소개

홍경란 한국병원약사회 2003 병원약사회지 Vol.20 No.2

신체적 운동수행에 따른 혈장의 효소활성도

김성환,이선장,전혜령,김학렬,최용어,홍경란 韓國體育大學校附屬 體育科學硏究所 1991 韓國體育大學校附屬 體育科學硏究所論文集 Vol.10 No.1

The purpose of this paper was to determine the difference of three method for suggestion to effective training method during gymnastic floor exercise. Elite trained women gymnastic players participated as subject of this research. Heart rate and plasma enzyme activity(LDH,CPK & GOT) were measured. Blood sample for analysis in plasma enzyme activity were drawn in the rest(before) and 10 min of recovery(after). LDH, GOT and CPK activity were measured as spectrophotometric and UV-Rate methods. It was not significant difference(p>.05) between three method in the heart rate, but heart rate in three method during gymnastic floor exercise was significantly increased. Also, Plasma enzyme activity expressed significantly increase after floor exercise, but not observed significant difference between three method. The results suggest that difference of method followed rest time not influenced in gymnastic floor exercise, because not observed significant difference between three methods.

現代 生活과 레크리에이션

홍경란 이화여자대학교 체육대학 1970 梨花體育學會誌 Vol.- No.4

장시간 싸이클 주행중 혈장 글루코스,젖산,소디움 및 포타시움 농도의 변화

김창근,김근우,김양수,김학렬,최용어,홍경란 韓國體育大學校附屬 體育科學硏究所 1991 韓國體育大學校附屬 體育科學硏究所論文集 Vol.10 No.1

장시간 최대하 싸이클 주행중 근피로를 파악하기 위한 지표로서 소디움, 포타시움 농도의 변화양상을 살펴보고자 대학 대표급 싸이클 선수 14명을 대상으로 롤러위에서 시속 40 - 41km의 싸이클 주행속도를 유지할 수 있도록 조절하여 120분동안 수행하도록 하였다. 혈장 글루코스, 젖산, 소이움 및 포타시움 농도의 측정을 위한 혈액쌤플은 새벽 6시 기상직후와 실험시작전(0), 운동 30분, 60, 90, 120분에 주전정맥에서 약 5ml의 혈액을 채혈하였다. 혈장 글루코스와 젖산농도는 spectrophotometer를 이용하여 효소법으로 측정하였으며 소디움, 포타시움 농도는 Flame photometer를 이용하여 standard method로 측정하였다. 혈장 글루코스와 포타시움 농도는 운동 전체를 통하여 큰 변화가 없는 것으로 나타났으며(P>.05) 젖산 및 소디움 농도는 운동의 시간경과에 따라 증가되는 경향(P<.05)을 나타내었으나 운동부하에 따른 변화폭은 적은 것으로 나타났다. 그러나 혈장 소디움 농도는 혈장의 젖산농도와 함께 유사한 변화양상을 나타냄으로서 다소의 관련성을 엿볼수 있어 이의 규명을 위한 계속적인 연구가 요구된다. The purpose of this study was to determine a significance of sodium (Na??) and potassium(K??) concentration with indicators for estimate a muscular fatigue during prolonged submaximal cycle race. Forteen endurance-trained cyclists were used as subjects in the study. The exercise protocol consisted cycle race for 120min on the rollers cycle. The blood sample for measurement of plasma glucose, lactate, sodium and potassium concentration were drawn to A.M.6:00, pre-cycling(0), 30, 60, 90, and 120 min of cycling by collected about 5ml in the antecubital vein. Plasma glucose and lactate concentration measured by enzymatic technique using spectrophotometer, also sodium and potassium concentration measured by standard method using flame photometer. Plasma glucose and potassium concentration was not significance difference(P>.05) during prolonged cycle exercise, but plasma lactate and sodium concentration was shown increase during time course of prolonged cycle race(P<.05). However, plasma lactate and sodium concentration shown increase of small range during cycle race. Sodium concentration was requested continuous research by shown simillar patterns with plasma lactate concentration.

고객만족과 친절서비스

홍경란 한국병원약사회 1998 병원약사회지 Vol.15 No.4

운동과 적혈구 노화와의 관계

최용어,윤탁영,홍경란 韓國體育大學校附屬 體育科學硏究所 1991 韓國體育大學校附屬 體育科學硏究所論文集 Vol.10 No.1

본 연구는 노화의 정도에 따라 적혈구를 분리하고 장시간의 운동 스트레스가 노화에 미치는 영향을 알아 보고자, 고도로 훈련된 남자 마라톤 선수 3명을 대상으로 HRmax 80% 수준에서 지속적인 런닝을 실시하였다. 전혈에서 RBC, WBC, MCV, Hb, Hct를 측정하고, 적혈구 내에 존재하는 Protein Carboxyl O-Methyltransferase(PCM)을 정제하여 그 activity를 측정하고, 적혈구 막을 분리하여 그 구성 단백질에 대한 PCM 의 Methylatability를 측정하여 운동과 적혈구 노화와의 관계를 설명하고자 한다. 각 시간별, Rest(0분), Running(30, 60, 90, 120분), Recovery(10, 30분)에 혈액을 채혈하여, 전혈에서 RBC, WBC, MCV, Hb, Hct의 변화를 조사하고 (Hematology Analyzer 5), 적혈구를 분리하여 50% Prcoll과 0.154M Hypaque에 넣고 22,000??g에서 40분간 원심분리하면 11 -15개 정도의 적혈구 층이 노화된 정도에 따라 분류된다. 적혈구 세포내의 PCM을 정제하고, 적혈구 막(ghost)을 분리하여 방사성 동위원소 (??C-SAM)를 methyl donor로 하여 PCM activity와 적혈구 막에 대한 Methylatability를 측정하였다. 런닝 30분에 RBC수가 증가하였으며, WBC와 Hb은 운동하는 동안 높은 수준을 유지하였다. MCV와 Hct는 변화가 없었다. Percoll과 Hypaque를 이용하여 노화된 정도에 따라 적혈구를 분류하였으나, 각 층에 대한 세포수의 측정이 뒤따르지 못하여 적혈구 수의 증가 혹은 감소와의 관계를 직접 볼수 없었다. 적혈구 내에서 정제된 PCM activity 는 운동 스트레스에 의한 변화를 보이지 않음으로써 항상 full을 유지하고 있음을 알 수 있다. 적혈구 막에 대한 Methlatability변화는 운동전 26.77±0.32 units/mg.min에서 런닝 30분에 34.29±0.55 units/mg.min로 큰 증가를 보였으며 런닝하는 동안 높은 수준을 유지하였다. 이는 운동 스트레스가 적혈구 막단백질을 구성하는 아미노산에 영향을 미쳐 비정상의 L-isoaspartyl 잔기를 만들었음을 말해주는 것이며, 이것이 노화된 단백질의 수복기전과 일치한다. 즉 PCM이 비정상의 L-isoaspartyl 잔기를 메칠화시켜 정상의 L-aspartyl잔기로 수복시킴으로써 운동 스트레스에 의한 영향을 극복하고자 하는 것이다. 그러므로 장시간의 운동 스트레스가 주는 영향이 노화에 의한 현상과 일치함을 알수 있다. Our studies showed the separation method of erythrocyte according to aging process and the effect that influenced a long-time exercise stress to aging. Highly trained marathon runners (n=3) performed a prolonged running(2hours) at the level of HRmax 80% RBC,WBC,MCV, Hct was measured in a whole blood.Protein Carboxyl O-Methyltransferase(PCM) in the erythrocyte was purified from the cytosol and the activity was measured. Methylatability of erythrocyte membrance protein was measured during marathon performance and recovery phase. Therefore, we interpreted the relation of exercise and erthrocyte aging. The count of RBC increased at the running 30 min. WBC and Hb maintained at the high level during marathon running. MCV and Hct did not changed. Erythrocyte divided into 11-15bands by Percoll and Hypaque according to aging. Because the erythrocyte did not count numerically in different age-related fraction of human erythrocyte, we cannot show the change of erythrocyte count. PCM activity purified in erythrocyte did not change by exercise stress, this suggests that PCM is full at all times. Methylatability of erythrocyte membrane protein changed from 26.77±0.32 units/mg.min before exercise to 34.29±0.55 units/mg.min at 30min after exercise and maintained a high level of methylatability during a running. This suggests that abnormal L-isoaspartyl residues came into being in the membrane protein composition as a result of exercise stress and are repaired by the same mechanism as aging repair that is, PCM methylated abnormal L-isoaspartyl residues in membrane protein and repaired into normal L-aspartyl residues, which removed the effect of exercise stress. Therefore, this study showed that the effect by exercise stress is the same as that of aging process.

Changes of in vitro Protein Carboxyl Methylation in Human Erythrocyte Membrane by Cell Aging and Damage

홍경란,전길자,Hong, Kyung-Ran,Jhon, Gil-Ja 생화학분자생물학회 1989 한국생화학회지 Vol.22 No.2

For the purpose of showing the physiological role of methyl esterification by protein carboxyl methyltransferase (PCM) as a post-translational modification, the intact human erythrocyte populations were separated by percoll/hypaque density gradient centrifugation according to erythrocyte aging. PCM activity in cytosolic fraction and methylatability of erythrocyte membrane was measured. The level of cytosolic PCM activity has shown insignificant change according to aging, but a twofold increase of methylation was observed in the oldest cells compared with the youngest ones. From this result, we could presume that abnormal protein as a substrate for the enzyme are formed during deamidation or oxidative damage events which accompany the aging process of protein molecules in cells. We observed the level of methylatability of membrane protein by chemical treatment. After heat treatment $(80^{\circ}C)$, base treatment (0.1N $NH_4OH$ at $37^{\circ}C$) or UV radiation, the extent of the methylation of the treated membrane also increased as a function of time. We also studied for the diabetic blood. The more increased Hb $A_{1c}$/total blood(%), the more increased methylatability of diabetic erythrocyte membrane. From these results, we could suggest that the ability of methyl esterification by PCM converts damaged protein into normal protein. Erythrocyte membrane protein was separated by CPC/PAGE in order to show protein participated in methylation. Band 4.5 was good substrate, methylatability of these proteins increased according to cell aging. Methylatabilities of band 3 and 4.5 were higher in diabetic blood. Protein carboxyl methyltransferase (PCM)에 의한 메칠화 반응의 생리적 기능을 알아보기 위해 percoll/hypaque density gradient centrifugation 방법으로 사람 적혈구를 노화된 정도에 따라 분류하고, 각 층에서 세포질내의 PCM 활성도와 적혈구 막의 methylatability를 관찰하였다. 그 결과 노화된 정도에 따라 적혈구 막의 methylatability는 증가하였고, 세포질내의 PCM 활성도는 거의 변화가 없었다. 또한 적혈구 막을 열 $(80^{\circ}C)$, 염기 (0.1 N $NH_4OH$) 또는 자외선으로 손상을 입힌 후 methylatability를 측정한 결과 시간의 함수로써 증가하였다. 당뇨병 환자 적혈구 막의 methylatability를 조사한 결과 Hb $A_{1c}$/total blood (%)가 커짐에 따라 methylatability가 증가하였다. 메칠화 반응에 관여하는 적혈구 막 단백질의 종류를 알아보기 위해 CPC/PAGE로 분리해 본 결과 band 4.5가 PCM에 대한 좋은 기질임이 확인되었고, 이들 단백질의 methylatability가 노화되어지는 정도에 따라 증가하였다. 당뇨병 환자의 경우 band 3와 band 4.5의 methylatability가 정상인에 비해 증가하였다. 이러한 결과로 볼 때, 이 효소에 대한 기질인 비정상 단백질이 적혈구 노화에 수반되는 deamidation이나 산화적 손상에 의해 증가됨을 제시해 볼 수 있다.

사람 적혈구 막에서 세포 노화와 손상에 의한 Protein Carboxyl Methylation 변화

홍경란,전길자 ( Kyung Ran Hong,Gil Ja Jhon ) 생화학분자생물학회 1989 BMB Reports Vol.22 No.2

For the purpose of showing the physiological role of methyl esterification by protein carboxyl methyltransferase (PCM) as a post-translational modification, the intact human erythrocyte populations were separated by percoll/hypaque density gradient centrifugation according to erythrocyte aging. PCM activity in cytosolic fraction and methylatability of erythrocyte membrane was measured. The level of cytosolic PCM activity has shown insignificant change according to aging, but a twofold increase of methylation was observed in the oldest cells compared with the youngest ones. From this result, we could presume that abnormal protein as a substrate for the enzyme are formed during deamidation or oxidative damage events which accompany the aging process of protein molecules in cells. We observed the level of methylatability of membrane protein by chemical treatment. After heat treatment (80℃), base treatment (0.1N NH₄OH at 37℃) or UV radiation, the extent of the methylation of the treated membrane also increased as a function of time. We also studied for the diabetic blood. The more increased Hb A_(lc)/total blood(%), the more increased methylatability of diabetic erythrocyte membrane. From these results, we could suggest that the ability of methyl esterification by PCM converts damaged protein into normal protein. Erythrocyte membrane protein was separated by CPC/PAGE in order to show protein participated in methylation. Band 4.5 was good substrate, methylatability of these proteins increased according to cell aging. Methylatabilities of band 3 and 4.5 were higher in diabetic blood.