전단지의 특성이 중국 소비자의 구매의도에 미치는 영향에 관한 연구

( Yong Qiang Ma ),( Seung Hee Im ),( Jin Hwan Hong ) 한국유통경영학회(구 한국유통정보학회) 2014 유통정보학회지 Vol.17 No.2

본 연구에서는 전단지를 활용한 촉진이 중국 소비자들의 구매의도에 어떤 영향을 미치는지에 대해서 두 차례의, 실험연구를 통해서 분석하였다.본 연구에서는 전단지의 제품 관련 속성과 촉진 관련속성을 선정하여, 두 차례의 실험 설계를 통해서 전단지를 활용한 촉진이 중국 소비자들의 지각된 가치와 지각된 위험, 그리고 구매의도에 미치는 영향을 조사하였다.첫 번째 실험연구에서는 전단지의 제품 관련 속성으로서 할인 폭과 특성광고의 효과를 살펴보았으며, 두 번째 실험연구에서는 전단지의 촉진 관련 속성으로 브랜드 프로모션 유무와 연관상품 촉진 유무에 대해서 2x2의 실험 설계를 통해 조사하였다. 연구 결과, 전단지의 특성을 활용한 촉진은 중국 소비자들의 구매의도에 유의한 영향을 미치는 것으로 나타났다. 반면 지각된 위험은 구매의도에 유의한 영향을 미치지 못하는 것으로 나타났는데, 이는 중국 소비자들이 가격을 더 중시하기 때문으로 추정할 수 있다. 마지막으로 이에 따른 시사점과 한계점들을 서술하였다. This study presents two experiments to investigate how store flyer promotion affects Chinese customers`` purchase intention. The particular interest is whether store flyers have different influences in different countries. This paper analyzes China, one of the world’s fastest-growing economies as a representative to explore the issue. Specifically, this study proposes a promotion and product characteristics for empirically analyzing the effect of store flyer promotion on Chinese consumers`` purchase intention. Tested through two experiments, the results demonstrate that store flyer promotion has a significant positive effect on Chinese consumers’ perceived value and purchase intention. These findings indicate that Chinese consumers are price-oriented rather than quality-oriented, and store flyer promotion are effective marketing tools for Chinese retailers.

Demethylation of CpG islands in the 5` upstream regions mediates the expression of the human testis-specific gene MAGEB16 and its mouse homolog Mageb16

( Yun Qiang Liu ),( Mei Ling Wang ),( Si Yuan Jiang ),( Yong Jie Lu ),( Da Chang Tao ),( Yuan Yang ),( Yong Xin Ma ),( Si Zhong Zhang ) 생화학분자생물학회 2014 BMB Reports Vol.47 No.2

Tissue-specific gene expression is regulated by epigenetic modification involving trans-acting factors. Here, we identified that the human MAGEB16 gene and its mouse homolog, Mageb16, are only expressed in the testis. To investigate the mechanism governing their expression, the promoter methy-lation status of these genes was examined in different samples. Two CpG islands (CGIs) in the 5` upstream region of MAGEB16 were highly demethylated in human testes, whereas they were methylated in cells without MAGEB16 expression. Similarly, the CGI in Mageb16 was hypomethylated in mouse testes but hypermethylated in other tissues and cells without Mageb16 expression. Additionally, the expression of these genes could be activated by treatment with the demethylation agent 5`-aza-2`- deoxycytidine (5`-aza-CdR). Luciferase assays revealed that both gene promoter activities were inhibited by methylation of the CGI regions. Therefore, we propose that the testis-specific expression of MAGEB16 and Mageb16 is regulated by the methylation status of their promoter regions. [BMB Reports 2014; 47(2): 86-91]

Effects of Dietary Acetyl-L-Carnitine on Meat Quality and Lipid Metabolism in Arbor Acres Broilers

Zhang, Yong,Ma, Qiugang,Bai, Xiumei,Zhao, Lihong,Wang, Qiang,Ji, Cheng,Liu, Laiting,Yin, Haicheng Asian Australasian Association of Animal Productio 2010 Animal Bioscience Vol.23 No.12

An experiment was conducted to evaluate the effects of dietary acetyl-L-carnitine (ALC) on growth performance, carcass characteristics, meat quality and lipid metabolism in broilers. A total of 240 one-day-old male Arbor Acres broilers were randomly allocated to 4 dietary treatments (0, 300, 600, and 900 mg/kg dietary ALC supplementation, respectively). Compared with the control treatment, addition of ALC resulted in lower (linear effect, p<0.05) ADG and AFI. Abdominal fat percentage decreased (linear effect, p<0.05) as dietary ALC was increased, but there was no effect on dressing percentage, breast muscle percentage or thigh muscle percentage. Breast muscle pH value 24 h post-mortem increased (linear effect, p<0.05), but there were no significant differences among treatments. However, thigh muscle pH value increased (linear effect, p<0.05) as dietary ALC was increased. Breast and thigh muscle $a^*$ values increased (linear effect, p<0.05), and breast and thigh muscle $b^*$ values decreased (linear effect, p<0.05) with increased ALC in the diet. In addition, breast and thigh muscle shear force value decreased (linear effect, p<0.05) as dietary ALC was increased. Total cholesterol, triglyceride, low-density lipoprotein cholesterol and lipoprotein lipase decreased (linear effect, p<0.05) and free fatty acid and lipase in serum increased (linear effect, p<0.05) with increased ALC in diets.

CLOCK and BMAL1 stabilize and activate RHOA to promote F-actin formation in cancer cells

Teng-jiao Ma,Zhi-wei Zhang,Yilu Lu,Ying-ying Zhang,Da-chang Tao,Yun-qiang Liu,Yong-xin Ma 생화학분자생물학회 2018 Experimental and molecular medicine Vol.50 No.-

Circadian genes control most of the physiological functions in cancer cells, including cell proliferation, migration, and invasion. The CLOCK and BMAL1 complex plays a central role in circadian rhythms. Previous studies have shown that circadian genes may act as oncogenes or tumor-suppressor genes. In addition, F-actin, regulated by RHOA, has been shown to participate in tumor progression. However, the roles of the CLOCK and BMAL1 genes in the regulation of tumor progression via the RHOA-ROCK-CFL pathway remain largely unclear. Here we first indicate that the rearrangement of F-actin is regulated by CLOCK and BMAL1. We found that CLOCK and BMAL1 can upregulate RHOA expression by inhibiting CUL3-mediated ubiquitination and activate RHOA by reducing the interaction between RHOA and RhoGDI. Consequently, CLOCK and BMAL1 control the expression of the components of the RHOA-ROCK-CFL pathway, which alters the dynamics of F-actin/G-actin turnover and promotes cancer cell proliferation, migration,

Promoter demethylation mediates the expression of ZNF645, a novel cancer/testis gene

( Gang Bai ),( Yun Qiang Liu ),( Hao Zhang ),( Dan Su ),( Da Chang Tao ),( Yuan Yang ),( Yong Xin Ma ),( Si Zhong Zhang ) 생화학분자생물학회 (구 한국생화학분자생물학회) 2010 BMB Reports Vol.43 No.6

Cancer/testis (CT) antigens exhibit highly tissue-restricted expression and are considered promising targets for cancer vaccines. Here we identified a novel CT gene ZNF645 which restrictively expresses in normal human testes and lung cancer patients (68.3%). To investigate the promoter methylation status of ZNF645, we carried out bisulfite genomic sequencing and found that the CpG island in its promoter was heavily methylated in normal lung tissues without the expression of ZNF645, whereas there was high demethylation in normal human testes and lung carcinoma tissues with its expression. Also ZNF645 could be remarkably activated in A549 and HEK293T cells treated by DNA demethylation agent 5`-aza-2`-deoxycytidine. And the dual luciferase assay revealed that the promoter activity of the ZNF645 was inhibited by methylation of the CpG island region. Therefore, we proposed that ZNF645 is a CT gene and activated in human testis and lung cancers by demethylation of its promoter region. [BMB reports 2010; 43(6): 400-406]

Centromere protein U enhances the progression of bladder cancer by promoting mitochondrial ribosomal protein s28 expression

Liu, Bei-Bei,Ma, Tao,Sun, Wei,Gao, Wu-Yue,Liu, Jian-Min,Li, Li-Qiang,Li, Wen-Yong,Wang, Sheng,Guo, Yuan-Yuan The Korean Society of Pharmacology 2021 The Korean Journal of Physiology & Pharmacology Vol.25 No.2

Bladder cancer is one of the most common types of cancer. Most gene mutations related to bladder cancer are dominantly acquired gene mutations and are not inherited. Previous comparative transcriptome analysis of urinary bladder cancer and control samples has revealed a set of genes that may play a role in tumor progression. Here we set out to investigate further the expression of two candidate genes, centromere protein U (CENPU) and mitochondrial ribosomal protein s28 (MRPS28) to better understand their role in bladder cancer pathogenesis. Our results confirmed that CENPU is up-regulated in human bladder cancer tissues at mRNA and protein levels. Gain-of-function and loss-of-function studies in T24 human urinary bladder cancer cell line revealed a hierarchical relationship between CENPU and MRPS28 in the regulation of cell viability, migration and invasion activity. CENPU expression was also up-regulated in in vivo nude mice xenograft model of bladder cancer and mice overexpressing CENPU had significantly higher tumor volume. In summary, our findings identify CENPU and MRPS28 in the molecular pathogenesis of bladder cancer and suggest that CENPU enhances the progression of bladder cancer by promoting MRPS28 expression.

JCAD deficiency attenuates activation of hepatic stellate cells and cholestatic fibrosis

Li Xie,Hui Chen,Li Zhang,Yue Ma,Yuan Zhou,Yong-Yu Yang,Chang Liu,Yu-Li Wang,Ya-Jun Yan,Jia Ding,Xiao Teng,Qiang Yang,Xiu-Ping Liu,Jian Wu 대한간학회 2024 Clinical and Molecular Hepatology(대한간학회지) Vol.30 No.2

Background/Aims: Cholestatic liver diseases including primary biliary cholangitis (PBC) are associated with active hepatic fibrogenesis, which ultimately progresses to cirrhosis. Activated hepatic stellate cells (HSCs) are the main fibrogenic effectors in response to cholangiocyte damage. JCAD regulates cell proliferation and malignant transformation in nonalcoholic steatoheaptitis-associated hepatocellular carcinoma (NASH-HCC). However, its participation in cholestatic fibrosis has not been explored yet. Methods: Serial sections of liver tissue of PBC patients were stained with immunofluorescence. Hepatic fibrosis was induced by bile duct ligation (BDL) in wild-type (WT), global JCAD knockout mice (JCAD-KO) and HSC-specific JCAD knockout mice (HSC-JCAD-KO), and evaluated by histopathology and biochemical tests. In situ-activated HSCs isolated from BDL mice were used to determine effects of JCAD on HSC activation. Results: In consistence with staining of liver sections from PBC patients, immunofluorescent staining revealed that JCAD expression was identified in smooth muscle α-actin (α-SMA)-positive fibroblast-like cells and was significantly up-regulated in WT mice with BDL. JCAD deficiency remarkably ameliorated BDL-induced hepatic injury and fibrosis, as documented by liver hydroxyproline content, when compared to WT mice with BDL. Histopathologically, collagen deposition was dramatically reduced in both JCAD-KO and HSC-JCAD-KO mice compared to WT mice, as visualized by Trichrome staining and semi-quantitative scores. Moreover, JCAD deprivation significantly attenuated in situ HSC activation and reduced expression of fibrotic genes after BDL. Conclusions: JCAD deficiency effectively suppressed hepatic fibrosis induced by BDL in mice, and the underlying mechanisms are largely through suppressed Hippo-YAP signaling activity in HSCs.

Effects of the Substitution of Mischmetal for PrNd on the Microstructures and Magnetic Properties of Rapidly Quenched (MM)PrNd-Fe-B Nanocrystalline Magnets

Zeng-ru Zhao,Xin Wang,Xue-feng Zhang,Qiang Ma,Yan-li Liu,Yong-feng Li,Fei Liu,Gao-feng Wang 한국자기학회 2017 Journal of Magnetics Vol.22 No.1

Mischmetal of Bayan Obo ore was utilized to prepare the high performance (Pr7.34Nd21.86)1-xMMxFe68.7Al0.1-Cu0.12Co0.88B ribbons using melt-spinning method. Phase composition and magnetic properties were investigated at room temperature. The ribbons mainly consist of R2Fe14B phase in isotropic nanostructure. Both coercivity and maximum energy product decrease with the increase of MM content. The magnetic parameters of the ribbons with MM = 20 % in mass are Br = 7.38 kGs, Hcj = 13.66 kOe, (BH)max = 11.81 MGOe. Henkel plots were applied to demonstrate the exchange coupling interaction between grains.

A novel CRX variant (p.R98X) is identified in a Chinese family of Retinitis pigmentosa with atypical and mild manifestations

Yingchuan Zhu,Hao Tan,Jiarong Zeng,Da-chang Tao,Yong-xin Ma,Yun-qiang Liu 한국유전학회 2019 Genes & Genomics Vol.41 No.3

Background Retinitis pigmentosa (RP) is the most common form of hereditary retinal degeneration that can cause inherited blindness. RP has extreme genetic and clinical heterogeneity, which brings a major obstacle to obtaining an accurate molecular diagnosis. Objective To analyze the genetic defect in a Chinese family of RP with a few atypical manifestations. Methods Whole-exome sequencing (WES) was applied to identify the disease-associated genes. Sanger sequencing was performed to validate the variants of candidate genes in the patient and his parents. In vitro expression analysis was further conducted to examine the potential biological function of the gene variant. Results A heterozygous nonsense variant c.292C > T (p.R98X) of CRX gene was identified to be present in the affected male. The c.292C > T variant of CRX was absent in all of the searched databases, including the 10,000 Chinese exome database. The nonsense variant was supposed to result in a truncated CRX protein with a destroyed homedomain (HD), which is essential for CRX translation. Interestingly, the following assay showed that the potential truncated protein was not detected, indicating that the variant may cause a loss-of-function mutation of CRX gene. Conclusion We identified a novel heterozygous null mutation in the CRX gene which was the first evidence of a nonsense mutation in the HD domain of CRX. Our finding suggested that the haploinsufficiency mutation of CRX gene contributed to the atypical and mild manifestations of the autosomal dominant RP in the Chinese family.

Zebrafish Dnd protein binds to 3`UTR of geminin mRNA and regulates its expression

( Shu Chen ),( Mei Zeng ),( Hua Qin Sun ),( Wen Qian Deng ),( Yi Lu Lu ),( Da Chang Tao ),( Yun Qiang Liu ),( Si Zhong Zhang ),( Yong Xin Ma ) 생화학분자생물학회 (구 한국생화학분자생물학회) 2010 BMB Reports Vol.43 No.6

Dnd (dead end) gene encodes an RNA binding protein and is specifically expressed in primordial germ cells (PGCs) as a vertebrate-specific component of the germ plasma throughout embryogenesis. By utilizing a technique of specific nucleic acids associated with proteins (SNAAP), 13 potential target mRNAs of zebrafish Dnd (ZDnd) protein were identified from 8-cell embryo, and 8 target mRNAs have been confirmed using an RT-PCR analysis. Of the target mRNAs, the present study is focused on the regulation of geminin, which is an inhibitor of DNA replication. Using electrophoretic mobility shift assay (EMSA), we demonstrated that ZDND protein bound the 67-nucleotide region from 864 to 931 in the 3`UTR of geminin mRNA, a sequence containing 60.29% of uridine. Results from a dual-luciferase assay in HEK293 cells showed that ZDND increases the translation of geminin. Taken together, the identification of target mRNA for ZDnd will be helpful to further explore the biological function of Dnd in zebrafish germ-line development as well as in cancer cells. [BMB reports 2010; 43(6): 438-444]