Inhibition of Notch1 signaling by Runx2 during osteoblast differentiation

Ann, Eun-Jung,Kim, Hwa-Young,Choi, Yun-Hee,Kim, Mi-Yeon,Mo, Jung-Soon,Jung, Jane,Yoon, Ji-Hye,Kim, Su-Man,Moon, Jeong-Sik,Seo, Mi-Sun,Hong, Ji-Ae,Jang, Won-Gu,Shore, Paul,Komori, Toshihisa,Koh, Jeong- Wiley (John WileySons) 2011 Journal of bone and mineral research Vol.26 No.2

Isolation and Identification of Various Microorganisms from Plastic Dumpsite Having Plastic Degradation Activities

Yeon-Hwa KIM,Ye-Rim SHIN,Jeong-Ann PARK,Suk-Jin HA 한국생물공학회 2023 한국생물공학회 학술대회 Vol.2023 No.10

Curcumin suppresses oncogenicity of human colon cancer cells by covalently modifying the cysteine 67 residue of SIRT1

Lee, Yeon-Hwa,Song, Na-Young,Suh, Jinyoung,Kim, Do-Hee,Kim, Wonki,Ann, Jihyae,Lee, Jeewoo,Baek, Jeong-Heum,Na, Hye-Kyung,Surh, Young-Joon Elsevier 2018 Cancer letters Vol.431 No.-

<P><B>Abstract</B></P> <P>SIRT1, an NAD<SUP>+</SUP>-dependent histone/protein deacetylase, has diverse physiological actions. Recent studies have demonstrated that SIRT1 is overexpressed in colorectal cancer, suggesting its oncogenic potential. However, the molecular mechanisms by which overexpressed SIRT1 induces the progression of colorectal cancer and its inhibition remain largely unknown. Curcumin (diferuloymethane), a major component of the spice turmeric derived from the plant <I>Curcuma longa</I> L., has been reported to exert chemopreventive and anti-carcinogenic effects on colon carcinogenesis. In the present study, we found that curcumin reduced the expression of SIRT1 protein without influencing its mRNA expression in human colon cancer cells, suggesting posttranslational regulation of SIRT1 by this phytochemical. Notably, ubiquitination and subsequent proteasomal degradation of SIRT1 were induced by curcumin treatment. Results of nano-LC-ESI-MS/MS revealed the direct binding of curcumin to cysteine 67 of SIRT1. In line with this result, the protein stability and clonogenicity of a mutant SIRT1 in which cysteine 67 was substituted by alanine were unaffected by curcumin. Taken together, these observations suggest that curcumin facilitates the proteasomal degradation of oncogenic SIRT1 through covalent modification of SIRT1 at the cysteine 67 residue.</P> <P><B>Highlights</B></P> <P> <UL> <LI> SIRT1 is overexpressed in colorectal cancer tissues compared with normal colon tissues. </LI> <LI> SIRT1 knockdown attenuates viability and migration of human colon cancer cells. </LI> <LI> Silencing of SIRT1 suppressed the growth of HCT-116 derived tumor xenografts. </LI> <LI> Curcumin inhibits migration and growth of HCT-116 cells. </LI> <LI> Curcumin covalently modifies SIRT1 on the cysteine 67 residue, thereby stimulating its proteasomal degradation. </LI> </UL> </P>

자간증의 변화 양상 (1953-1998)

강춘화(Choon Hwa Kang),이지연(Ji Yeon Lee),최미영(Mi Young Choi),박민혜(Min Hye Park),안현숙(Hyun Sook Ann) 대한산부인과학회 1999 Obstetrics & Gynecology Science Vol.42 No.9

목적: 1953년에서 1998년까지 46년동안 부산 일신기독병원에서의 자간증의 변화 양상을 알아보기 위함이다. 연구 방법: 1953년 1월 1일부터 1998년 12월 31일까지 부산 일신기독병원 산부인과에서 분만한 233,613명 산모중 1910예의 자간 환자의 의무기록 자료를 중심으로 자간의 발생빈도, 분만에 따른 자간의 발생시기, 자간의 처치방법, 모성 사망률 및 주산기 사망률을 조사하였다. 결과: 자간증의 발생빈도는 10,000명 분만당 81.8이었다. 연도별로 보면 그 발생빈도는 1953-1962년 137.3/10,000에서 1963-1972년 278.4/10,000로 증가하였다가 이후 계속 감소하여 1993-1998년 6.5/10,000였으며 1973년부터 1992년까지의 현저한 감소에 비해 마지막 6년동안은 서서히 감소하는 추세이다. 분만에 따른 자간의 발생시기는 분만전 자간이 54%, 분만중 자간이 29%, 분만후 자간이 17%였고 분만전 자간환자의 비율이 점차 감소하면서 분만중, 그리고 분만후 자간환자의 비율이 상대적으로 증가하였다. 자간으로 인한 모성 사망은 59예로 모성사망률은 3.1%이었다. 1953-1962년 11.1%에서 1963-1972년 3.8%로 모성 사망률의 가장 큰 감소가 있었고, 1986년 이후로는 한명의 모성사망도 없었다.분만후 자간에서 분만전 혹은 분만중 자간보다 높은 사망률을 보였다. 주산기 사망은 사산 156예, 신생아 사망 124예로 주산기 사망률은 1,000 분만당 144.1이었다. 주산기 사망률은 1953-1962년 243.2/1,000에서 1963-1972년 141.5/1,000로 의미있게 감소하였으나 1982년 이후로는 서서히 증가하는 추세이다. 결론: 지난 46년간 산전 진찰 및 자간 치료의 발달과 함께 자간의 발생빈도와 자간으로 인한 모성 사망률이 현저히 감소하였다. 그러나 자간은 아직도 높은 모성 사망률 및 주산기 사망률을 보이는 심각한 임신중 합병증으로 남아있다. Objectives: To determine changes in the incidence and pattern of eclampsia in Il Sin Christian Hospital over a 46-year period. Methods: Information was collected from medical records of the 1910 eclamptic patients among 233,613 deliveries in Il Sin Christian Hospital from Jan. 1 1953 to Dec. 31 1998. Incidence, presentation, and management of eclampsia were reviewed retrospectively, and maternal mortality rate and perinatal mortality rate were calculated. Statistical analysis was done by Chi-squared and Fisher’s exact test through two by two tables looking at relative changes between each study period. Results: The overall incidence of eclampsia was 81.8 per 10,000 deliveries. The incidence of eclampsia had increased from 137.3/10,000 in 1953-1962 to 278.4/10,000 in 1963-1972, but the rate had reduced to 6.5/10,000 in 1993-1998. There was a statistically significant fall in the rate of eclampsia every decade between 1973 and 1992, but there has been steady decrease in the last study period. Convulsion occurred antepartum in 54% of patients, intrapartum in 29% and postpartum in 17%. With the reduction in the proportion of antepartum eclampsia, there has been a relative increase in that of intrapartum and postpartum eclampsia. Maternal death occurred in 59 cases among eclampsia, and maternal mortality rate was 3.1%. Maternal mortality rate had significantly decresed from 11.1% in 1953-1962 to 3.8% in 1963-1972, and there has been no maternal death from eclampsia since 1986. Postpartum eclampsia had increased death risk compared with antepartum or intrapartum eclampsia. There were 280 cases of perinatal death and overall perinatal mortality rate was 144.1 per 1000 deliveries. There was a significant decrease in the rate from 243.2/1000 in 1953-1962 to 141.5/1000 in 1963-1972, but the rate has risen steadily since 1983. Conclusions: With the improvement in antenatal care and management of eclampsia, the incidence of eclampsia and its associated maternal mortality has decreased over the last 46 years. But eclampsia still remains a significant complication of pregnancy with high maternal and perinatal mortality.

LC : Side Population in LX2 Cells Decreased by TGF-β

( Jong Bin Kim ),( Yeon Hwa Ann ),( Seo Young Park ),( Hyeon Gun Jee ),( Hye Ri Kim ),( Jeong Hoon Lee ),( Su Jong Yu ),( Hyo Suk Lee ),( Yoon Jun Kim ) 대한간학회 2013 춘·추계 학술대회 (KASL) Vol.2013 No.1

Aim: Side population (SP) cells are known to be enriched in stem/progenitor-like cells. Transforming growth factor (TGF)-β signaling is associated with ECM production in hepatic stellate cells. We hypothesized that the SP fraction in LX2 cells is associated with ECM deposition, which is regulated through TGF-β signaling. Methods: We investigated the relationship between SP cells and TGF-β signaling in the hepatic stellate cell line LX2. The effects of TGF-β and SB431542 on the SP fraction and expression of collagen type I and phospho-Smad2 was determined. Results: We identified 0.8.3% SP cells in LX2 cells. The growth rate of sorted SP and non-SP cells was similar to that of the original LX2 population, but population of the G0/G1 phase was increased in SP cells. Treatment of LX2 cells with TGF-β decreased the SP fraction in a dose-dependent manner and increased the production of collagen type I. Treatment of LX2 cells with SB431542 blocked the effect of TGF-β on the SP fraction and the expression of collagen type I. We cultured LX2 cells on collagen-coated dishes to observe the effect of ECM deposition on the SP fraction. The growth rate and cell cycle distribution was similar to that observed on normal tissue culture dishes, but the SP fraction was decreased when LX2 cells were cultured on collagen-coated plates. Conclusions: These results show that LX2 cells contain a SP fraction and that TGF-β signaling is involved in the induction of ECM deposition as well as the number of SP cells.

Serum- and glucocorticoid-inducible kinase 1 (SGK1) controls Notch1 signaling by downregulation of protein stability through Fbw7 ubiquitin ligase.

Mo, Jung-Soon,Ann, Eun-Jung,Yoon, Ji-Hye,Jung, Jane,Choi, Yun-Hee,Kim, Hwa-Young,Ahn, Ji-Seon,Kim, Su-Man,Kim, Mi-Yeon,Hong, Ji-Ae,Seo, Mi-Sun,Lang, Florian,Choi, Eui-Ju,Park, Hee-Sae Cambridge University Press 2011 Journal of cell science Vol.124 No.1

<P>Notch is a transmembrane protein that acts as a transcriptional factor in the Notch signaling pathway for cell survival, cell death and cell differentiation. Notch1 and Fbw7 mutations both lead the activation of the Notch1 pathway and are found in the majority of patients with the leukemia T-ALL. However, little is known about the mechanisms and regulators that are responsible for attenuating the Notch signaling pathway through Fbw7. Here, we report that the serum- and glucocorticoid-inducible protein kinase SGK1 remarkably reduced the protein stability of the active form of Notch1 through Fbw7. The protein level and transcriptional activity of the Notch1 intracellular domain (Notch1-IC) were higher in SGK1-deficient cells than in SGK1 wild-type cells. Notch1-IC was able to form a trimeric complex with Fbw7 and SGK1, thereby SGK1 enhanced the protein degradation of Notch1-IC via a Fbw7-dependent proteasomal pathway. Furthermore, activated SGK1 phosphorylated Fbw7 at serine 227, an effect inducing Notch1-IC protein degradation and ubiquitylation. Moreover, accumulated dexamethasone-induced SGK1 facilitated the degradation of Notch1-IC through phosphorylation of Fbw7. Together our results suggest that SGK1 inhibits the Notch1 signaling pathway via phosphorylation of Fbw7.</P>

慶山地域에서 分離된 Yersinia屬菌의 特性에 關한 硏究

鄭永建,權五陳,安莊連,梁成鎬,孫東和 영남대학교 자원문제연구소 1989 資源問題硏究 Vol.8 No.-

Yersinia spp. were isolated from the beef, the pork and the intestine of wild mice and investigated the various properties and incidence. The results obtained were as follows: Among 58 isolates, only 2 strains(PM-1, PM-2) were identified as Yersinia spp. with 3.4% of isolation rate. No species were iaolated from the beef and the intestine of wild mice. 『 Yersinia spp. Showed great resistance to lincomycin, cephaloxyl and PM-2 was more resisted to these antibiotics than PM-1. These 2 strains were very sensitive to heating and NaCl solution.

암 치료에서의 침술의 가치

Choi, Jung-Eun,Cho, Chong-Kwan,Lee, Yeon-Weol,Yoo, Hwa-Seung,Lu, Weidong,Dean-Clower, Elizabeth,Doherty-Gilman, Anne,Rosenthal, David S. 대한암한의학회 2010 大韓癌韓醫學會誌 Vol.15 No.1

암 환자에의 침술 임상 연구는 종양학에서 새롭고 도전적인 분야이다. 임상 연구 결과는 환자와 종양학자들을 위한 임상적으로 타당한 답변을 우리에게 지속적으로 제공할 것이다. 침은 환자들에게 그들의 치료 계획에 활동적으로 참가할 수 있는 능력을 주면서 암과 암 치료관련 증상을 다루는데 안전하고 유효한 치료법이라는 일반적으로 이용될 수 있는 증거가 제시되었다. 미래의 연구는 임상 연구가, 임상의사, 그리고 환자의 연루를 요구한다. 혁신적 연구 방법의 발전 역시 중요하다. 더 많은 증거가 지속적으로 나오면서 종양 침술이 결국에는 종양학적 치료행위의 표준에 통합될 것이 기대된다. DFCI와 기타 주요 암 센터와 같은 주요 대학병원과 연구기관 에서의 침술의 성공적인 통합은 암 치료에 있어 침의 가치와 소용을 강조한다. Clinical research on acupuncture in cancer care is a new and challenging field in oncology. The results of clinical research will continue to provide clinically relevant answers for patients and oncologists. The evidence currently available has suggested that acupuncture is a safe and effective therapy to manage cancer and treatment related symptoms, while giving patients the ability to actively participate in their own care plan. The article explains the potential benefits of acupuncture and describes the difficulties in studying its effectiveness.

대구지역 사업체급식 메뉴 유형별 염도 및 소금함량 측정

김지애(Ji Ae Kim),김윤화(Yun Hwa Kim),안문영(Moon Young Ann),이연경(Yeon Kyung Lee) 대한지역사회영양학회 2012 대한지역사회영양학회지 Vol.17 No.5

The purpose of this study was to investigate salinity and sodium content in foods served at industry foodservice operations in selected workplaces in Daegu. The researcher collected 1,142 food items categorized into 22 menu item groups from lunch samples served at 100 industry foodservice operations and measured the salinity of the food items, and calculated sodium and salt contents from the salinity. In terms of salt content of one meal at each workplace, 46% of the meals were 3-<5 g, and only 3.0% of them were 1.7 g or less, which is one third of the daily target intake (5 g) presented by the Dietary Reference Intakes for Koreans. In terms of sodium content, 35.3% of the meals were 1,000-< 1,500 mg (most), and only 2.5% of them were 667 mg or less, which is, again, one third of the daily target intake. As to the salinity of each menu item group, side dishes such as sauces, kimchi, seasoned vegetables, hardboiled foods, and stir-fried foods were significantly higher, but the sodium content was not higher, because their quantity per serving was small. The salinity of foods with liquid such as stews, noodles, soups, and broths was relatively lower, but the sodium content was higher, because their quantity per serving was large. This indicates that an education program should be developed for foodservice workers who prepare food for the workplace regarding the development and promotion of various menus for reducing sodium content, in order to reduce sodium content in meals served by food service in the workplace.