만성관절염과 루푸스의 피로, 통증, 및 통증효능감에 관한 연구

임난영,이은영,양용숙,정순애,차경옥,이여진 대한류마티스 건강전문학회 2000 근관절건강학회지 Vol.7 No.1

This study was designed to distinguish by the characteristic difference and the degree of symptoms such as fatigue, pain, coping to pain, and efficacy on pain, and to offer descriptive data for nursing intervention for improving coping ability to pain along each characteristics of disease in chronic arthritis and systemic lupus erythematosus. The subjects were 135 outpatients in the hospital for rheumatic disease in H-University, Seoul. The data were collected by structural questionnaire, from April 29 to June 29, 1999. The results were that the fatigue score was high in the OA patients while the RA patients and SLE patients experienced middle range of fatigue score, but which was not statistically different. Although the RA patients felt higher pain than other diseases, they have well coped with their pain than the others. In efficacy on pain the SLE patients had higher score than others but all of the disease showed lower score. No statistically significant difference among the three croup was recorded in efficacy on pain. Therefore, pain management in the RA patients was primary nursing intervention because they felt severe pain and have well coped with pain while they had lower pain efficacy score than the others. It is also important that fatigue management and coping strategies on pain for the OA patients and SLE patients are specially supportive in the nursing intervention.

Tat-NOL3 protects against hippocampal neuronal cell death induced by oxidative stress through the regulation of apoptotic pathways

SOHN, EUN JEONG,SHIN, MIN JEA,EUM, WON SIK,KIM, DAE WON,YONG, JI IN,RYU, EUN JI,PARK, JUNG HWAN,CHO, SU BIN,CHA, HYUN JU,KIM, SANG JIN,YEO, HYEON JI,YEO, EUN JI,CHOI, YEON JOO,IM, SEUNG KWON,KWEON, HA Spandidos Publications 2016 INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE Vol.38 No.1

<P>Oxidative stress-induced apoptosis is associated with neuronal cell death and ischemia. The NOL3 [nucleolar protein 3 (apoptosis repressor with CARD domain)] protein protects against oxidative stress-induced cell death. However, the protective mechanism responsible for this effect as well as the effects of NOL3 against oxidative stress in ischemia remain unclear. Thus, we examined the protective effects of NOL3 protein on hydrogen peroxide (H2O2)-induced oxidative stress and the mechanism responsible for these effects in hippocampal neuronal HT22 cells and in an animal model of forebrain ischemia using Tat-fused NOL3 protein (Tat-NOL3). Purified Tat-NOL3 protein transduced into the H2O2-exposed HT22 cells and inhibited the production of reactive oxygen species (ROS), DNA fragmentation and reduced mitochondrial membrane potential (Delta(Psi m)). In addition, Tat-NOL3 prevented neuronal cell death through the regulation of apoptotic signaling pathways including Bax, Bcl-2, caspase-2, -3 and -8, PARP and p53. In addition, Tat-NOL3 protein transduced into the animal brains and significantly protected against neuronal cell death in the CA1 region of the hippocampus by regulating the activation of microglia and astrocytes. Taken together, these findings demonstrate that Tat-NOL3 protein protects against oxidative stress-induced neuronal cell death by regulating oxidative stress and by acting as an anti-apoptotic protein. Thus, we suggest that Tat-NOL3 represents a potential therapeutic agent for protection against ischemic brain injury.</P>

Tat-Thioredoxin-like protein 1 attenuates ischemic brain injury by regulation of MAPKs and apoptosis signaling

Hyun Ju Cha,Won Sik Eum,Gi Soo Youn,Jung Hwan Park,Hyeon Ji Yeo,Eun Ji Yeo,Hyun Jung Kwon,Lee Re Lee,Na Yeon Kim,Su Yeon Kwon,Yong-Jun Cho,Sung-Woo Cho,Oh-Shin Kwon,Eun Jeong Sohn,Dae Won Kim,Duk-Soo 생화학분자생물학회 2023 BMB Reports Vol.56 No.4

Thioredoxin-like protein 1 (TXNL1), one of the thioredoxin superfamilyknown as redox-regulator, plays an essential in maintainingcell survival via various antioxidant and anti-apoptoticmechanisms. It is well known that relationship between ischemiaand oxidative stress, however, the role of TXNL1 protein inischemic damage has not been fully investigated. In the presentstudy, we aimed to determine the protective role of TXNL1against on ischemic injury in vitro and in vivo using cellpermeable Tat-TXNL1 fusion protein. Transduced Tat-TXNL1inhibited ROS production and cell death in H2O2-exposedhippocampal neuronal (HT-22) cells and modulated MAPKsand Akt activation, and pro-apoptotic protein expression levelsin the cells. In an ischemia animal model, Tat-TXNL1 markedlydecreased hippocampal neuronal cell death and the activationof astrocytes and microglia. These findings indicate thatcell permeable Tat-TXNL1 protects against oxidative stress invitro and in vivo ischemic animal model. Therefore, we suggestTat-TXNL1 can be a potential therapeutic protein for ischemicinjury.

Evaluation for the Safety of Bee Venom in ICR Mice

Hu Jang Lee, Chun-Nam Cha, Yeo-Eun Lee, In-Jin Kang, Suk Kim 충북대학교 동물의학연구소 2012 Journal of Biomedical and Translational Research Vol.13 No.1

This study was conducted in order to examine the safety of bee venom as an alternative for antibiotics using male ICR mice. Five-week-old male mice received a single intravenous injection of a dried honey bee venom at the concentration of 0.25 mg/kg (a clinical dose) or 0.5 mg/kg through the tail vein and various pathophysiological analyses were performed after three days. No significant differences in changes of body weight were observed between the saline-treated control group and the experimental groups. In the hematological analysis, none of the parameters were affected by bee venom. In blood biochemistry analysis, none of the markers were affected by administration of bee venom. Similarly, there were no significant effects on markers for liver, kidney, and skeletal muscle functions in all treated- groups. On macroscopic examination, no remarkable lesions were detected in these organs. Because there were no adverse effects of the bee venom in a single intravenous toxicity test for three days, it was concluded that bee venom could be a candidate for a safe natural antibiotic for use in the animal production industry.

Therapeutic Effect of Dioctahedral Smectite on Diarrhea Caused by E. coli and Salmonella in Calves

Hu Jang Lee1,3*, Chun-Nam Cha, Yeo-Eun Lee, Hyunju Choi, Joon-Seob Shin, Suk Kim 충북대학교 동물의학연구소 2012 Journal of Biomedical and Translational Research Vol.13 No.2

The objectives of the current study were to evaluate the therapeutic effect of dioctahedral smectite (smectite) against calf diarrhea caused by pathogenic E. coli and/or Salmonella typhimurium. In this study, 20 calves (aged 2~3 months) with diarrhea were used for evaluation of the efficacy of smectite on calf diarrhea with 20% smectite suspension in PBS. Calves received 10 ml smectite suspension three times per day after feeding, and fecal samples were collected at the gate of treatment and on the first, second, third, fourth, and fifth day after administration. On the fifth day after treatment with smectite suspension, the diarrhea index showed a significant decrease in the treated group, compared to the control group (P<0.001). The number of pathogenic E. coli in feces of the treated group was significantly decreased, compared to each control group from the second day after treatment (P<0.001), and that of Salmonella typhimurium was significantly decreased from the first day after treatment (P<0.05). According to the results of the current study, 20% smectite suspension had a therapeutic effect on diarrhea caused by E. coli and/or Salmonella in calves.

Molecular Mapping of Resistant Genes to Brown Planthopper, Bphl and bph2, in Rice

Cha, Young-Soon,Cho, Yong-Gu,Shin, Kyeong-Og,Yeo, Un-Sang,Choi, Jae-Eul,Eun, Moo-Young The Korean Society of Crop Science 1999 Korean journal of crop science Vol.44 No.4

This study was carried out to map Bphl and bph2 gene in Mudgo and Sangju13 (Oryza sativa L.) respectively conferring resistance to brown plan-thopper (BPH) and to establish the marker-assisted selection (MAS) system. Bulked seedling (grown for 20 days) test was conducted with the 73 F4 lines derived from a cross between Nagdongbyeo and Mudgo for Bphl and with 53 BC3F5 lines derived from the Milyang95/Sangju13 cross for bph2. Bph1 was mapped between RG413 and RG901 on chromo-some 12 at a distance of 7.5 cM from RG413 and 8.4 cM from RG90l. A recessive gene bph2 was located near RZ76 on chromosome 12 at a distance of 14.4 cM. Bphl and bph2 were linked to each other with a distance of about 30 cM. An RFLP marker, RG413 linked to Bphl, was converted to an STS marker to facilitate the marker-assisted selection. BPH resistant genotypes could be selected with 92% accuracy in a population derived from a line of NM47-B-B.

Bactericidal Efficacy of Vital-Oxide^®, Disinfectant Solution Against Salmonella Typhimurium and Brucella Ovis

Cha, Chun-Nam,Lee, Yeo-Eun,Kang, In-Jin,Yoo, Chang-Yeul,An, Sun-Jeong,Kim, Suk,Lee, Hu-Jang The Korean Society of Food Hygiene and Safety 2012 한국식품위생안전성학회지 Vol.27 No.1

살모넬라와 부루셀라는 가축에 심각한 질병을 유발하며, 축산업과 식품산업에 많은 경제적 손실을 초래하고 있다. 본 연구에서는, 이산화염소와 베타인 염산염을 주성분으로 한 소독제 바이탈옥사이드의 $Salmonella$ $typhimurium$과 $Brucella$ $ovis$에 대한 효력시험을 수행하였다. 배지희석법을 이용한 살균효력시험은 $4^{\circ}C$에서 30분 동안 시험 세균을 희석 소독제에 노출시켜 소독제의 가장 효과적인 낮은 희석배수를 결정하는 시험이다. 바이탈옥사이드와 시험 세균들을 처리조건에 따라 증류수, 경수, 그리고 유기물 등으로 희석하여 반응을 시켰다. 유기물 조건에서, $Salmonella$ $typhimurium$과 $Brucella$ $ovis$에 대한 바이탈옥사이드의 살균력은 경수조건에서의 살균력과 비교하여 낮게 나타났는데, 이는 유기물들에 의한 소독제의 살균 유효성분에 대한 저해작용에 따른 것으로 생각된다. 바이탈옥사이드는 $Salmonella$ $typhimurium$과 $Brucella$ $ovis$와 같은 가축병원성 질병들에 대해 살균효과를 갖고 있기 때문에, 이 소독제는 세균성 질병의 확산을 제어하는데 이용될 수 있을 것으로 사료된다. $Salmonella$ spp. and $Brucella$ spp. have caused a considerable disease of farmed animals and economic loss in animal farming and food industry. In this study, the disinfection efficacy of Vital-$Oxidel^{(R)}$, a commercial disinfectant, composed to chlorine dioxide, betaine hydrochloride, and propylene glycol was evaluated against $S.$ $typhimurium$ and $Brucella$ $ovis$. A bactericidal efficacy test by broth dilution method was used to determine the lowest effective dilution of the disinfectant following exposure to test bacteria for 30 min at $4^{\circ}C$. Vital-$Oixdel^{(R)}$ and test bacteria were diluted with distilled water (DW), hard water (HW) or organic matter suspension (OM) according to treatment condition. On OM condition, the bactericidal activity of Vital-$Oixdel^{(R)}$ against S. typhimurium and Brucella ovis was lowered compared to that on HW condition. As Vital-$Oxidel^{(R)}$ possesses bactericidal efficacy against animal pathogenic bacteria such as $S.$ $typhimurium$ and $Brucella$ $ovis$, this disinfectant solution can be used to control the spread of bacterial diseases.

Antiviral Efficacy of Citra-kill^®, Disinfectant Solution Against Avian Influenza Virus

Cha, Chun-Nam,Lee, Yeo-Eun,Kang, In-Jin,Yoo, Chang-Yeul,Park, Eun-Kee,An, Sun-Jeong,Kim, Suk,Lee, Hu-Jang The Korean Society of Food Hygiene and Safety 2012 한국식품위생안전성학회지 Vol.27 No.1

본 연구에서는 4급 암모늄에 속하는 quaternary ammonium chloride와 구연산의 합제인 Citra-$Kill^{(R)}$의 조류인플루엔자에 대한 살바이러스 효과를 확인하기 위해 국립수의과학검역원의 소독제 효력시험 중 바이러스 소독제 효력시험에 따라 수행하였다. 소독제와 조류인플루엔자바이러스를 증류수, 경수, 그리고 유기물 조건에서 반응시킨 후, 중화액을 이용하여 중화시킨 다음, 중화된 용액 0.2 ml를 10일령의 계태아 요막에 주입하여 5일 동안 배양시킨 다음, 요막액을 채취하여 바이러스의 생존여부를 혈구응집반응을 통해 확인하여 소독제의 효력배수를 결정하였다. 본 연구의 결과, Citra-$Kill^{(R)}$은 증류수, 경수, 그리고 유기물 조건에서 각각 2,000, 1,500, 그리고 500배에서 조류인플루엔자바이러스를 불활성화시켜, 조류인플루엔자에 대해 뛰어난 소독효과를 갖고 있는 것이 확인되었다. 따라서 향후, 야외적용시험을 통해 실제적용에 따른 효과를 확인할 필요가 있을 것으로 사료된다. Highly pathogenic avian influenza virus (HPAIV) is already panzootic in poultry and caused a considerable economic loss in poultry industry. In addition, HPAIV continues to cross species barriers to infect humans and other mammals, often with fatal outcomes. In this study, the virucidal efficacy of Citra-$Kill^{(R)}$ composed to quaternary ammonium chloride and citric acid was investigated against avian influenza H9N2 virus (AIV). A virucidal efficacy was determined with the viability of AIV contacted with the disinfectant in the allantoic membrane of chicken embryos. Citra-$Kill^{(R)}$ and AIV was reacted on the distilled water (DW), hard water (HW) or organic matter suspension (OM) condition. On DW condition, AIV was inactivated with 2,000 fold dilutions of Citra-$Kill^{(R)}$. When the antiviral effect on HW condition was evaluated, the antiviral activity of the disinfectant showed on 1,500 fold dilutions against AIV. With the investigation of the antiviral effect of the disinfectant on OM condition, AIV was inactivated on 500 fold dilutions of Citra-$Kill^{(R)}$. As Citra-$Kill^{(R)}$ possesses virucidal efficacy against AIV, the disinfectant solution can be used to limit the spread of animal viral diseases.

Bactericidal Efficacies of an Aquatic Disinfectant Tablet Composed to Calcium Hypochlorite Against Vibrio anguillarum and Streptococcus iniae

Cha, Chun-Nam,Lee, Yeo-Eun,Kang, In-Jin,Yoo, Chang-Yeul,Choi, Hyun-Ju,Kim, Suk,Lee, Hu-Jang The Korean Society of Food Hygiene and Safety 2012 한국식품위생안전성학회지 Vol.27 No.3

Vibrio spp. and Streptococcus spp. have caused a considerable disease of farmed fish and economic loss in fish farming and seafood industry. In this study, the efficacy of an aquatic disinfectant tablet composed to calcium hypochlorite was evaluated against V. anguillarum and S. iniae. A bactericidal efficacy test by broth dilution method was used to determine the lowest effective dilution of the disinfectant following exposure to test bacteria for 30 min at $4^{\circ}C$. An aquatic disinfectant tablet and test bacteria were diluted with distilled water (DW), hard water (HW) or organic matter suspension (OM) according to treatment condition. V. anguillarum on the DW, HW and OM condition was completely inactivated with 16,000 15,000 and 13,000 fold dilutions of the disinfectant, respectively. On the DW, HW and OM condition, S. iniae was absolutely inactivated with 17,000 16,000 and 14,000 fold dilutions of the disinfectant, respectively. As an aquatic disinfectant tablet possesses bactericidal efficacy against fish pathogenic bacteria such as V. anguillarum and S. iniae this disinfectant solution can be used to control the spread of fish infective bacterial diseases.

The role of allergic rhinitis in the development of otitis media with effusion : Effect on eustachian tube function

Yeo, Seung Geun,Park, Dong Choon,Eun, Young Gyu,Cha, Chang Il WHO COLLABORATING CENTRE FOR TRADITIONAL MEDICINE 2007 東西醫學硏究所 論文集 Vol.2007 No.-

Purpose: The pathogenesis of otitis media with effusion(OME) is considered multifactorial, with viral upper respiratory tract infection and eustachian tube dysfunction. Allergy may be related to the pathogenesis of OME or to another etiological factor. We investigated the role of allergic rhinitis(AR) in children with OME and evaluated eustachian tube function in patients with AR. Materials and methods: We prospectively analyzed the prevalence of AR. serum eosinophil count and serum total IgE concentrations in 123 children with OME and in 141 controls. IgE concentration in middle ear effusion was compared in children with OME with and without AR, and eustachian tube function after a nasal provocation test was compared between patients with AR and controls. Results: The prevalence of AR in children with OME (28.4%) and control subjects (24.1 %) did not differ significantly. These 2 groups also showed no differences in total eosinophil count and serum and middle car effusion IgE concentration. Abnormalities in eustachian tube function were the same in patients with AR and controls. Conclusions: Allergic rhinitis may not be related to the development of OME in children.