Time-dependent Expression of CXCR3 Ligands and IFN-γ in Tuberculosis Infection

( Wou Young Chung ),( Hye Eun Byeon ),( Kwang Joo Park ) 대한결핵 및 호흡기학회 2021 대한결핵 및 호흡기학회 추계학술대회 초록집 Vol.129 No.-

Interferon (IFN)-γ and CXCR3 ligands play important roles in host defence against tuberculosis (TB) infection. We previously reported the significant increased of CXCR3 ligands levels in TB antigen-stimulated blood of TB patients compared to latent TB infection (LTBI) as well as healthy controls. Venous blood of the control group (n = 12), LTBI patient group (n = 12), and active TB patients (n = 12) was drawn and stimulated with TB-specific antigens. After 0, 1, 2, 8, and 24 h of incubation, lymphocytes and monocytes were isolated. The mRNA expression levels IFN-γ and CXCR3 ligands (CXCL9, CXCL10, amd CXCL11) were evaluated using real time RT-PCR, and their concentrations in supernatant were measured using ELISA. Also, mRNA expression of CXCR3, CCR3, and TNF-α was evaluated. The Results showed that time-dependent increases of lymphocyte mRNA levels of CXCR3 ligands were more prominent than those of IFN-γ in TB patients. Protein level measurements of them in supernatant showed the similar Results. The protein concentrations and mRNA levels of CXCR3 ligands in TB patients were significantly higher in TB patients than those in LTBI and the controls, while those of IFN-γ levels were not different among groups. The ratio of lymphocyte mRNA levels of CXCR9, CXCL10, CXCL11, and IFN-γ at 24 h in TB patients relative to LTBI patients were 2.4, 2.0, 3.1, and 1.5, respectively. In conclusion, transcription activation levels of CXCR3 ligands were more prominent than those of IFN-γ in TB patients. The measurement of transcription levels of CXCR3 ligands could differentiate TB patients and LTBI patients.

미만성 거대 B형 세포 림프종 환자에서 CHOP 항암 치료와 Filgrastim 투여 후 발생한 폐쇄세기관지기질화폐렴 1례

정우영 ( Wou Young Chung ),변민광 ( Min Kwang Byun ),이진형 ( Jin Hyoung Lee ),한창훈 ( Chang Hoon Hahn ),강신명 ( Shin Myung Kang ),김진석 ( Jin Seok Kim ),조상호 ( San Ho Cho ),김영삼 ( Young Sam Kim ),김세규 ( Se Kyu Kim ),장 대한결핵 및 호흡기학회 2005 Tuberculosis and Respiratory Diseases Vol.59 No.5

기관지동맥 색전술 후 객혈의 재발에 관한 연구

정우영 ( Wou Young Chung ),변민광 ( Min Kwang Byun ),박무석 ( Moo Suk Park ),한창훈 ( Chang Hoon Hahn ),강신명 ( Shin Myung Kang ),이도연 ( Do Yon Lee ),김영삼 ( Young Sam Kim ),김세규 ( Se Kyu Kim ),김성규 ( Sung Kyu Kim ),장준 대한결핵 및 호흡기학회 2006 Tuberculosis and Respiratory Diseases Vol.60 No.1

Nuclear factor E2-related factor 2 Dependent Overexpression of Sulfiredoxin and Peroxiredoxin 3 in Human Lung Cancer

( Young Sun Kim ),( Hye Lim Lee ),( Ki Bum Lee ),( Joo Hun Park ),( Wou Young Chung ),( Keu Sung Lee ),( Seung Soo Sheen ),( Kwang Joo Park ),( Sung Chul Hwang ) 대한내과학회 2011 The Korean Journal of Internal Medicine Vol.26 No.3

Background/Aims: Oxidative stress results in protein oxidation and is implicated in carcinogenesis. Sulfiredoxin (Srx) is responsible for the enzymatic reversal of inactivated peroxiredoxin (Prx). Nuclear factor E2-related factor 2 (Nrf2) binds to antioxidant responsive elements and upregulates the expression of Srx and Prx during oxidative stress. We aimed to elucidate the biological functions and potential roles of Srx in lung cancer. Methods: To study the roles of Srx and Prx III in lung cancer, we compared the protein levels of Nrf2, Prxs, thioredoxin, and Srx in 40 surgically resected human lung cancer tissues using immunoblot and immunohistochemical analyses. Transforming growth factor-β1, tumor necrosis factor-α, and camptothecin treatment were used to examine Prx III inactivation in Mv1Lu mink lung epithelial cells and A549 lung cancer cells. Results: Prx I and Prx III proteins were markedly overexpressed in lung cancer tissues. A significant increase in the oxidized form of a cysteine sulfhydryl at the catalytic site of Prxs was found in carcinogenic lung tissue compared to normal lung tissue. Densitometric analyses of immunoblot data revealed significant Srx expression, which was higher in squamous cell carcinoma tissue (60%, 12/20) than in adenocarcinoma (20%, 4/20). Also, Nrf2 was present in the nuclear compartment of cancer cells. Conclusions: Srx and Prx III proteins were markedly overexpressed in human squamous cell carcinoma, suggesting that these proteins may play a protective role against oxidative injury and compensate for the high rate of mitochondrial metabolism in lung cancer.

A Case of Juvenile Spring Eruption of the Ears

Park, Young Min,Kim, Tae Yoon,Kim, Chung Won,Chae, Koo Seog,Kim, Jin Wou 대한피부과학회 1997 Annals of Dermatology Vol.9 No.2

Juvenile spring eruption(JSE) of the ears is an unusual type of photodermatosis, which develops on the light exposed areas of the ears of boys and young male adults in the early spring months. JSE has received little attention in the literature, and to our knowledge no cases have been reported in Korea until now. Herein we report a case of JSE occurring in a 17-year-old man who has suffered from a recurrent pruritic erythematous papulovesicular eruption of both helix, followed by crusting and healing without scarring within one to two months early each spring for six years.

한국어판 세인트조지 호흡기설문의 타당도와 신뢰도 검정

김영삼 ( Young Sam Kim ),변민광 ( Min Kwang Byun ),정우영 ( Wou Young Jung ),정재희 ( Jae Hee Jeong ),최상봉 ( Sang Bong Choi ),강신명 ( Shin Myung Kang ),문지애 ( Ji Ae Moon ),한숙정 ( Suk Jung Han ),남정모 ( Chung Mo Nam ),박무 대한결핵 및 호흡기학회 2006 Tuberculosis and Respiratory Diseases Vol.61 No.2

연구배경: 만성폐질환 환자에서 삶의 질을 측정하는 도구로서 세인트조지 호흡기 설문(St George`s Respiratory Questionnaire;SGRQ)이 개발되어 치료효과를 판정하는 측정지표로 널리 사용되고 있으나 영어권국가에서 만들어 졌기 때문에 우리나라에서 동일하게 사용하는데 제한점을 가지고 있다. 따라서 이의 정확한 적용을 위해서 언어, 기술, 개념적인 관점을 고려하여 한국어 설문지를 만들고 이들의 타당도와 신뢰도를 평가하였다. 방법: 한국판 세인트조지 호흡기 설문은 번역, 역번역, 전문가 패널의 수정을 통해 완성되었고, 세브란스병원 호흡기내과를 방문한 환자를 대상으로 하여 이 설문을 시행하였다. 신뢰도 검정은 Cronbach`s α를 구하여 측정하였고 타당도는 폐기능검사, 6분보행검사, 호흡곤란지수와 산소포화도 검사를 동시에 시행하여 설문 검사결과와의 상관관계를 알아보았다. 결과: 74명의 환자를 대상으로 시행하였으며, 남녀 비는 2.2:1, 평균연령은 63.8±10.3세였다. 이 중 천식환자는 27명(36.5%), 만성폐쇄성폐질환 환자는 39명 (52.7%)이었고, 결핵에 의한 파괴 폐환자는 8명(10.8%)이었다. Cronbach`s α 값은 증상영역, 활동영역, 영향영역 및 총 점수에서 각각 0.63, 0.87, 0.89, 0.92 이였다. 총점수와 FEV1, FVC, 6분보행거리, 산소포화도, 호흡곤란지수와 유의한 상관관계를 보였다. 결론: 한국어판 세인트조지 호흡기 설문은 만성폐질환 및 천식과 같은 만성폐질환 환자에서 신뢰도가 높았고 총점수와의 상관관계는 6분보행검사 및 산소포화도에서 높음을 알수 있었고 폐기능 검사와의 상관계수는 원저인 세인트조지 호흡기설문 및 타 국가의 번역본 호흡기설문에서와 같이 높지 않음을 알 수 있었다. 따라서 우리나라에서도 호흡기질환의 삶의 질에 대한 치료효과 판정에 활용할 수 있을 것으로 기대된다. Background: The health-related quality of life (HRQL) for patients with chronic respiratory disease has been emphasized, because chronic respiratory disease (CRD) is chronic and progressive, and it finally causes disability. HRQL instruments may be useful for monitoring patients` progress or for determining the most appropriate choice of treatment. We describe the adapting St George`s Respiratory Questionnaire (SGRQ), which is a self-administered questionnaire developed by Jones et al. (1991), into the Korean version for covering three domains of health for the patients suffering with airways disease. Method: We obtained the original SGRQ from the author after gaining permission. For adaptation, we created an expert panel and translated the original questionnaire into Korean language. The translated questionnaire was then back-translated by bilingual experts and we compared it with the original questionnaire. After correction and feasibility testing, 74 patients with chronic respiratory disease (COPD, asthma, destroyed lung) completed the Korean version of the SGRQ. The clinical status of each patients was evaluated concurrently with measurement of their health status. Result: The Korean version of the SGRQ was acceptable and easy to understand. Cronbach`s alpha reliability coefficient was 0.92 for the overall scale and 0.63 for the Symptoms, subscale, 0.87 for the Activity, subscale, and 0.89 for the Impacts subscales. The correlation coefficients between the overall score and the Borg scale score, oxygen saturation, and forced expiratory volume in one second (FEV1) were 0.52, -0.32 and -0.26, respectively. These results support that the Korean SGRQ was correlated with other measurements. Conclusion: The Korean SGRQ was reliable and valid for patients with chronic respiratory disease, such as COPD, asthma, and destroyed lung. The SGRQ score was well correlated with other respiratory measurements as well. Although further studies should complete the adaptation work, our results suggest that the SGRQ may be used in Korea and also for international studies involving Korean CRD patients. (Tuberc Respir Dis 2006; 61: 121-128)

The Significance of Sedation Control in Patients Receiving Mechanical Ventilation

Jung, Yun Jung,Chung, Wou Young,Lee, Miyeon,Lee, Keu Sung,Park, Joo Hun,Sheen, Seung Soo,Hwang, Sung Chul,Park, Kwang Joo The Korean Academy of Tuberculosis and Respiratory 2012 Tuberculosis and Respiratory Diseases Vol.73 No.3

Background: Adequate assessment and control of sedation play crucial roles in the proper performance of mechanical ventilation. Methods: A total of 30 patients with various pulmonary diseases were prospectively enrolled. The study population was randomized into two groups. The sedation assessment group (SAG) received active protocol-based control of sedation, and in the empiric control group (ECG), the sedation levels were empirically adjusted. Subsequently, daily interruption of sedation (DIS) was conducted in the SAG. Results: In the SAG, the dose of midazolam was significantly reduced by control of sedation (day 1, $1.3{\pm}0.5{\mu}g/kg/min$; day 2, $0.9{\pm}0.4{\mu}g/kg/min$; p<0.01), and was significantly lower than the ECG on day 2 (p<0.01). Likewise, on day 2, sedation levels were significantly lower in the SAG than in the ECG. Significant relationship was found between Ramsay sedation scale and Richmond agitation-sedation scale (RASS; $r_s$=-0.57), Ramsay Sedation Scale and Bispectral Index (BIS; $r_s$=0.77), and RASS and BIS ($r_s$=-0.79). In 10 patients, who didn't require re-sedation after DIS, BIS showed the earliest and most significant changes among the sedation scales. Ventilatory parameters showed significant but less prominent changes, and hemodynamic parameters didn't show significant changes. No seriously adverse events ensued after the implementation of DIS. Conclusion: Active assessment and control of sedation significantly reduced the dosage of sedatives in patients receiving mechanical ventilation. DIS, conducted in limited cases, suggested its potential efficacy and tolerability.

Short-term Evaluation of a Comprehensive Education Program Including Inhaler Training and Disease Management on Chronic Obstructive Pulmonary Disease

( Kwang Ha Yoo ),( Wou Young Chung ),( Joo Hun Park ),( Sung Chul Hwang ),( Tae-eun Kim ),( Min Jung Oh ),( Dae Ryong Kang ),( Chin Kook Rhee ),( Hyoung Kyu Yoon ),( Tae-hyung Kim ),( Deog Kyeom Kim ) 대한결핵 및 호흡기학회 2017 Tuberculosis and Respiratory Diseases Vol.80 No.4

Background: Proper education regarding inhaler usage and optimal management of chronic obstructive pulmonary disease (COPD) is essential for effectively treating patients with COPD. This study was conducted to evaluate the effects of a comprehensive education program including inhaler training and COPD management. Methods: We enlisted 127 patients with COPD on an outpatient basis at 43 private clinics in Korea. The patients were educated on inhaler usage and disease management for three visits across 2 weeks. Physicians and patients were administered a COPD assessment test (CAT) and questionnaires about the correct usage of inhalers and management of COPD before commencement of this program and after their third visit. Results: The outcomes of 127 COPD patients were analyzed. CAT scores (19.6±12.5 vs. 15.1±12.3) improved significantly after this program (p<0.05). Patients with improved CAT scores of 4 points or more had a better understanding of COPD management and the correct technique for using inhalers than those who did not have improved CAT scores (p<0.05). Conclusion: A comprehensive education program including inhaler training and COPD management at a primary care setting improved CAT scores and led to patientsc better understanding of COPD management.

The Significance of Sedation Control in Patients Receiving Mechanical Ventilation

( Yun Jung Jung ),( Wou Young Chung ),( Miyeon Lee ),( Keu Sung Lee ),( Joo Hun Park ),( Seung Soo Sheen ),( Sung Chul Hwang ),( Kwang Joo Park ) 대한결핵 및 호흡기학회 2012 Tuberculosis and Respiratory Diseases Vol.73 No.3

Background: Adequate assessment and control of sedation play crucial roles in the proper performance of mechanical ventilation. Methods: A total of 30 patients with various pulmonary diseases were prospectively enrolled. The study population was randomized into two groups. The sedation assessment group (SAG) received active protocol-based control of sedation, and in the empiric control group (ECG), the sedation levels were empirically adjusted. Subsequently, daily interruption of sedation (DIS) was conducted in the SAG. Results: In the SAG, the dose of midazolam was significantly reduced by control of sedation (day 1, 1.3±0.5 μg/kg/min; day 2, 0.9±0.4μg/kg/min; p<0.01), and was significantly lower than the ECG on day 2 (p<0.01). Likewise, on day 2, sedation levels were significantly lower in the SAG than in the ECG. Significant relationship was found between Ramsay sedation scale and Richmond agitation-sedation scale (RASS; rs=-0.57), Ramsay Sedation Scale and Bispectral Index (BIS; rs=0.77), and RASS and BIS ( rs=-0.79). In 10 patients, who didn`t require re-sedation after DIS, BIS showed the earliest and most significant changes among the sedation scales. Ventilatory parameters showed significant but less prominent changes, and hemodynamic parameters didn`t show significant changes. No seriously adverse events ensued after the implementation of DIS. Conclusion: Active assessment and control of sedation significantly reduced the dosage of sedatives in patients receiving mechanical ventilation. DIS, conducted in limited cases, suggested its potential efficacy and tolerability.

Selectively Decreased Expression of Peroxiredoxins Induced by Silica in Pulmonary Epithelial Cells

( Hye Lim Lee ),( Young Sun Kim ),( Joo Hun Park ),( Wou Young Chung ),( Kyu Sung Lee ),( Yoon Jung Oh ),( Seung Soo Sheen ),( Kwang Joo Park ),( Sung Chul Hwang ) 대한내과학회 2009 The Korean Journal of Internal Medicine Vol.24 No.3

Background/Aims: Peroxiredoxin (Prx) belongs to a ubiquitous family of antioxidant enzymes that regulates many cellular processes through intracellular oxidative signal transduction pathways. Silica-induced lung damage involves reactive oxygen species (ROS) that trigger subsequent toxic effects and inflammatory responses in alveolar epithelial cells resulting in fibrosis. Therefore, we investigated the role of Prx in the development of lung oxidant injury caused by silicosis, and determined the implication of ROS in that process. Methods: Lung epithelial cell lines A549 and WI26 were treated with 1% silica for 0, 24, or 48 hours, following pretreatment of the A549 cells with N-acetyl-L-cysteine and diphenylene iodonium and no pretreatment of the WI26 cells. We transfected an HA-ubiquitin construct into the A549 cell line and then analyzed the cells via Western blotting and co-immunoprecipitation. Results: Silica treatment induced cell death in the A549 lung epithelial cell line and selectively degraded Prx I without impairing protein synthesis in the A549 cells, even when the ROS effect was blocked chemically by Nacetyl-L-cysteine. A co-immunoprecipitation study revealed that Prx I did not undergo ubiquitination. Conclusions: Silica treatment induces a decrease of Prx I expression in lung epithelial cell lines regardless of the presence of ROS. The silica-induced degradation of Prx does not involve the ubiquitin-proteasomal pathway. (Korean J Intern Med 2009;24:220-226)