http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Complexity in regulation of microRNA machinery components in invasive breast carcinoma.
Kwon, Sun Young,Lee, Jae-ho,Kim, Bora,Park, Jong-Wook,Kwon, Taeg Kyu,Kang, Sun Hee,Kim, Shin Science Press ; W.B. Saunders 2014 PATHOLOGY ONCOLOGY RESEARCH Vol.20 No.3
<P>Altered expression of microRNA (miRNA) machinery components may play an important role in breast cancer progression. The objective of the current study was to evaluate Drosha, the DiGeorge syndrome critical region gene 8 (DGCR8), Dicer, and Argonaute 2 (AGO2) mRNA expression in invasive breast carcinoma (IBC) and to assess the value of clinical parameters on their expression. By using quantitative real-time PCR, we examined the expression of the four miRNA machinery components in 52 breast tumor tissues which are diagnosed as invasive ductal carcinoma and adjacent non-neoplastic tissues. In the present study, decreased mRNA expression levels of major miRNA machinery components were observed in IBC. The altered mRNA expression levels of DGCR8 and AGO2 are positively correlated with to each other. This study revealed for the first time that expression alterations of DGCR8 are significantly associated with estrogen receptor and Ki-67 status in IBC. Moreover, AGO2 mRNA expression level was significantly correlated with N stage. These results provided evidences that down-regulated the four miRNA machinery components may play an important role in breast pathobiology and that DGCR8 and AGO2 might be associated with important clinical factors.</P>
Kwon, Woo-Taeg,Lee, Woo-Sik,Park, Pyo-Jam,Park, Tae-Kyu,Kang, Hyun The Korean Society for Microbiology and Biotechnol 2012 Journal of microbiology and biotechnology Vol.22 No.11
Japanese encephalitis virus (JEV) envelope (E) protein holds great promise for use in the development of a recombinant vaccine. Purified recombinant E (rE) protein may be useful for numerous clinical applications; however, there are limitations in using the Escherichia coli expression system for producing high-quality rE protein. Therefore, in this study, the yeast expression system was used to generate the rE protein. For protein production using the yeast system, the full-length JEV E gene was cloned into Pichia pastoris. SDS-PAGE and immunoblotting analysis demonstrated that the rE protein had a molecular mass of 58 kDa and was glycosylated. The predicted size of the mature unmodified E protein is 53 kDa, suggesting that post-translational modifications resulted in the higher molecular mass. The rE protein was purified to greater than 95% purity using combined ammonium sulfate precipitation and a SP-Sepharose Fast Flow column. This purified rE protein was evaluated for immunogenicity and protective efficacy in mice. The survival rates of mice immunized with the rE protein were significantly increased over that of Hyphantria cunea nuclear polyhedrosis virus E protein (HcE). Our results indicate that the rE protein expressed in the P. pastoris expression system holds great promise for use in the development of a subunit vaccine against JEV.
Escherichia coli의 시티딘/디옥시시티딘 디아미나제를 코드하는 cdd 유전자의 클로닝
권택규,김태호,황선갑,김종국,송방호,홍순덕 한국산업미생물학회 1990 한국미생물·생명공학회지 Vol.18 No.6
E. coli의 cytidine deaminase(cytidine/2'-deoxycytidine aminohydrolase; EC 3.5,4.5)를 코딩하는 cdd 유전자를 E. coli cdd^-pyr^- 결손 변이주를 cloning host로 하여 southern blotting과 colony hybridization을 통하여 클로닝하였다. cdd 유전자가 단편은, cdd 유전자의 transcription initiation 부위의 23개 nucleotide를 합성한 후 probe로 사용하여 Southern hybridization에 의해 회수된 cdd 유전자를 함유한 단편을 얻었으며, 이를 pBR322에 삽입한 후 형질전환하여 colony hybridization한 결과 cdd^+ cell을 얻었다. 삽입된 DNA 단편의 size는 27kb이었으며 이를 결실 및 subcloning을 연속 수행한 결과 2.1kb의 SalI/DraI tragment(pTK605)에 cdd 유전자가 location되어 있음을 알게 되었다. Mini cell 실험결과 합성된 polypeptide는 약 33kDa이었으며, wild type의 cytidine deaminase의 활성이 pBR322에서 증폭시킴으로서 37배 정도 배가되었으며, pBR322에 비해 pUC vector계에서 다시 활성이 7배 정도 증가됨을 알 수 있었다. We have cloned the cdd gene from E. coli C600 using (cdd^-) as a host. From the sequenced promoter region of E. coli cdd gene which has been determined by Valentin-Hansen P. (1985), we synthesized the 23 mer oligonucleotides corresponding to the transcription initiation region and used as a probe for cloning of the cdd gene by Southern blotting. The isolated fragments in the blotting were introduced to the colony hybridization after transforming it into the E. coli JF611 (cdd^-, pyr^- double mutant), and we identified the hybridized band at 27 kb long. From the original insert of 27 kb fragment in the BamHI site of pBR322, the 5.3 kb fragment containing the cdd gene was isolated by subsequent deletion and subcloning. From the derived plasmid pTK509, further deletion and subcloning were performed and clarified that the cdd gene was located in the 2.1 kb of SalI/DraI segment in the insert of pTK605. The polypeptide encoded by the cloned DNA was appeared to be a molecular mass of 33,000.
남부지방에서 들깨의 파종기가 종실수량과 지방산 조성에 미치는 영향
권병선,현규환,임준택 순천대학교 1999 순천대학교 자연과학논문집 Vol.18 No.2
To determine optimal sowing date of perilla in southern region of Korea, perilla cv. Okdongdlggae cultivar was grown under three different sowing date. The results are as follows: Yield components such as number of branches, number of spike per plant and number of capsule per spike were the highest at the sowing date of June 10. Plant at June 10 showed the highest in 1,000 seed weight and grain yield. As plant were grown under later sowing time, they showed higher values in content of oil, oleic acid and linolenic acid and lower values in content of plamitic acid such as stearic acid and linoleic acid.
임준택(June Taeg Lim),임정묵(Jung Mook Lim),권병선(Byung Sun Kwon),신동영(Dong Yeong Shin),현규환(Kyu Hwan Hyun),김학진(Hak Jin Kim),정순주(Soon Ju Chung),이범선(Beom Seon Lee) 한국자원식물학회 2006 한국자원식물학회지 Vol.19 No.2
본 연구는 여러 오이 품종들의 억제작형에서의 생장과 수량을 조사하여 이 작형에서 적합한 품종을 선발하기 위해 수행하였다. 엽수에 있어서 청장계 오이가 가장 많았고 흑진주계 오이에서 가장 적어 영양생장으로는 청장계 오이가 가장 좋았다. 암꽃수에 있어서 흑진주계 오이가 가장 많았고 청장계 오이가 가장 적었다. 암꽃비율에서도 흑진주계 오이가 가장 높았으며 청장계 오이가 가장 낮았다. 퇴화 암꽃비율에서는 흑진주계 오이가 가장 높았으며 청장계 오이는 0%로 거의 퇴화를 보이지 않았다. 측지제거처리와 측지무제거 처리간 수량은 유이한 차이가 없었다. 수량에 있어서 흑진주계 오이가 가장 높았고 청장계 오이가 가장 낮아 억제작형재배에서 가장 적합한 품종은 흑진주계 오이인 것으로 나타났다. This study was conducted to select proper varieties of cucumber by comparing growth and yield in retarding cultivation under hydroponic culture. Number of leaves appeared to be the highest Chungjangoi and the lowest in Heukjinjuoi. Heukjinjuoi showed higher vegetative growth rate. Heukjinjuoi showed the highest number of female flowers while Chungjangoi the lowest. The ratio of female flower was the highest in Heukjinjuoi and the lowest in Chungjangoi. Abortion of female flower appeared to be the highest in Heukjinjuoi and the lowest with 0% in Chungjangoi. There was no significant difference in yield between treatments of removal and not-removal of lateral buds.
Plastic house의 형태 및 재배시기의 차이에 따른 오이 품종들의 생식형질 및 수량반응
임준택(June Taeg Lim),임정묵(Jung Mook Lim),권병선(Byung Sun Kwon),신동영(Dong Young Shin),현규환(Kyu Hwan Hyun),김학진(Hak Jin Kim),정순주(Soon Ju Chung),이범선(Beom Seon Lee) 한국자원식물학회 2006 한국자원식물학회지 Vol.19 No.2
본 연구는 2종류의 하우스, 즉 1-2W 형 하우스와 무기둥하우스에, 시설억제재배, 촉성계배, 반측성재배 그리고 여름재배의 4 재배시기에 8개의 오이 품종을 재식하여, 재배시기에 따른 품종들의 암수꽃의 발생, 개체 당 과실수에 따른 퇴화 과실수, 그리고 수량을 조사 비교하여 각 작형에 알맞은 품종들을 추천하기 위해 수행하였다. 시설억제재배에서는 낙합계나 흑진주계 또는 입추백다다기오이가 생육 및 수량이 양호하였고, 촉성재배에서는 겨우살이청장오이, 입추백다다기오이 그리고 가을낙합오이가 추천된다. 반측성재배에서는 청장계 오이나 낙합계 오이가 비교적 양호한 수량을 보였으며 여름재배에서는 남부청장오이나 가을낙합오이가 양호하였다. This study was conducted to determine proper varieties of cucumber at four different cultural seasons. Eight varieties of cucumber from four varietal groups were grown in two types of plastic house, 1-2W type and postless type, at four different cultural seasons, retarding culture, forcing culture, semi-forcing culture and summer culture. Number of female flowers, male flowers, aborted female flowers, leaves and fruits per plant and fresh weight of fruits per plant were observed. The results were listed as follows. Varietal group of Nakhap or Hukjinjoo and ibchubakdadakioi showed better growth and higher yield at retarding culture. Kyeusalichungjangoi, ibchubdadakioi and Gaulnakhapoi could be recommended as proper varieties at forcing culture. Varietal group of Chungjang or Nakhap showed relatively higher yield at semi-forcing culture. Naboochungjangoi and Gaulnakhapoi were recommended as proper varieties at summer culture.