한열변증에 따른 중풍 환자의 제반특성 비교

김민경,이인환,신애숙,김나희,김혜미,심소라,나병조,조승연,박성욱,정우상,문상관,박정미,고창남,조기호,김영석,배형섭,Kim, Min-kyung,Lee, ln-whan,Shin, Ae-sook,Kim, Na-hee,Kim, Hye-mi,Shim, So-ra,Na, Byung-Jo,Cho, Seung-yeon,Park, Seong-uk,Jung, Woo Sang 대한중풍순환신경학회 2010 대한중풍.순환신경학회지 Vol.11 No.1

Object : This study was conducted as part of the national project to standardize stroke diagnosis in Korean medicine. In this study, we aimed to evaluate the characteristics of acute stroke between heat pattern group and cold pattern group. Methods : We recruited stroke patients from 5 universities(Kyung-Hee University oriental medical center, Kyung-Hee University East-West Neo medical center, Kyungwon university lncheon oriental medical center, Kyungwon university Songpa oriental medical center and DongGuk university llsan oriental medical center) from April, 2007 until February, 2010. We diagnosed them and selected 463 heat pattern patients and 182 cold pattern patients. Results : We find that the risk factor of smoking, alcohol, diet(prefer to meat) are more associated with the heat pattern group. On the other hand, inflammation history in recent 3 months and diet(prefer to sea food) are more associated with the cold pattern group. RBC, Hg, Hct, TG, CK and Cl are more related to the heat pattern group. CPT, total cholesterol and HDL are more related to the cold pattern group. Tae-eum type takes high distribution of the heat pattern group, and So-eum type takes high distribution of the cold pattern group. Conclusions : This study provides evidence that the heat pattern group have more risk factor than the cold pattern group.

Determinant of Arterial Stiffness in Young Adults

Jo Yoon-Kyung,Jeon Justin Y.,Kim Eun-Sung,Jekal Youn-Suk,Eom Yong-Bin,Im Jee-Aee The Korean Society for Biomedical Laboratory Scien 2006 Journal of biomedical laboratory sciences Vol.12 No.3

Cardiovascular disease (CVD) risk factors may be acting several decades before CVD becomes manifest. Data from young subjects may be valuable in further elucidating at this issue. We evaluated the association between baPWV (brachial-ankle pulse wave velocity) and cardiovascular risk factors in apparently healthy young adults. A total of 46 male and 91 female adolescents aged $18{\sim}25 years$ were studied. baPWV increased in a dose-responsive manner as the number of metabolic syndrome components. In both gender groups, baPWV was positively correlated with age. In males, waist, circumference total cholesterol, and LDL-cholesterol were positively correlated with baPWV, and in females, blood pressure (BP) was positively correlated with baPWV. Age, gender, mean BP, and Homeostasis model assessment insulin resistance (HOMA-IR) were found to be independent factors associated with baPWV levels. In conclusion, mean BP, age, gender, and HOMA-IR were associated with baPWV in young adults. This result suggests that multiple cardiovascular risk factors may be associated with an increased risk of arterial stiffness in young adults.

IBEM analyses on half-cell potential measurement for NDE of rebar corrosion

Kyung, Je-Woon,Tae, Sung-Ho,Lee, Han-Seung,Alver, Yalcin,Yoo, Jo-Hyeong TECHNO-PRESS 2007 COMPUTERS AND CONCRETE Vol.4 No.4

Corrosion of Reinforcement (rebar) is nondestructively estimated by the half-cell potential measurement. As is the case with other nondestructive testings (NDT), understanding of the underlying principles should be clarified in order to obtain meaningful results. Therefore, the measurement of potentials in concrete is analytically investigated. The effect of internal defects on the potentials measured is clarified numerically by the boundary element method (BEM). Thus, a simplified inversion by BEM is applied to convert the potentials on concrete surface to those on rebars, taking into account the concrete resistivity. Because the potentials measured on concrete surface are so sensitive to moisture content, concrete resistivity and surface condition, an inverse procedure to convert the potentials on concrete surface into those on rebars is developed on the basis of BEM. It is found that ASTM criterion is practically applicable to estimate corrosion from the potential values converted. In experiments, an applicability of the procedure is examined by accelerated corrosion tests of reinforced concrete (RC) slabs. For practical use, the procedure is developed where results of IBEM are visualized by VRML (Virtual Reality modeling Language) in three-dimensional space.

Splenic Absorption of Radiopharmaceutical in Systemic Bone Scans Performed after Liver Transplantation

Sang-Hyeong Kil,Kyung-Nam Jo,Yung-Hyun Lim The Korean Society of Nuclear Medicine Technology 2023 핵의학 기술 Vol.27 No.1

Technetium-labeled phosphate bone scan was shown to detect bone fractures and bone metastasis in early stage than general radiographs. Therefore, bone scan has become one of the most frequently performed nuclear medicine imaging examination. However, non-osseous radiopharmaceutical uptake on the bone scan are unusual findings. We report a case of diffuse splenic absorption of Tc-99m dicarboxypropane diphosphonate in patients who undergo liver transplantation.

Reduction of Renal Fibrosis as a Result of Liposome Encapsulated Clodronate Induced Macrophage Depletion after Unilateral Ureteral Obstruction in Rats

Sung, Su Ah,Jo, Sang Kyung,Cho, Won Yong,Won, Nam Hee,Kim, Hyoung Kyu S.Karger 2006 The Nephron Journals Vol.105 No.1

<P><I>Background/Aim:</I> Macrophages have been thought to play a role in renal tubulointerstitial fibrosis; recent reports have demonstrated an antifibrotic effect of macrophages in late-stage renal fibrosis. Liposome-encapsulated clodronate (LC) produces a selective and systemic depletion of phagocytic macrophages in vivo. To study the role of initial infiltrating macrophages in renal fibrosis, we compared the effects of pretreatment with LC and a liposome vehicle for control of the severity of renal fibrosis in a unilateral ureteral obstruction (UUO) rat model. <I>Methods:</I> One day after a single intravenous injection of LC or liposome vehicle, the rats underwent UUO. Following 1, 5, and 14 days, the kidneys were examined to evaluate macrophage infiltration and renal fibrosis. <I>Results:</I> LC depleted macrophages systemically and reduced renal fibrosis associated with UUO; this beneficial effect was accompanied by a decrease of transforming growth factor beta mRNA expression. The osteopontin expression was also reduced by pretreatment with LC. <I>Conclusion:</I> Initial interstitial infiltration of macrophages contributes to tubulointerstitial fibrosis in UUO.</P><P>Copyright © 2007 S. Karger AG, Basel</P>

A Framework to Develop E-Learning Contents of Agricultural Safety

( Kyung Sun Lee ),( Sung Feel Jo ),( Kyeong Hee Choi ),( Inseok Lee ),( Yong Ku Kong ),( Kyung Suk Lee ),( Myung Chul Jung ) 한국감성과학회 2014 춘계학술대회 Vol.2014 No.-

This paper presented a framework to develop e-learning contents of agricultural safety for farmers in Korea. The framework was divided into 1) Selection of crops and educational methods, 2) Collection of materials, and 3) Development of e-learning contents. In the first phase, the crops were selected based on the data of production, farming type, farmhouses, production area, and the educational methods were chosen considering the characteristics of age, education, learning interest, etc. The second phase, collection of materials, was associated with characteristics and risk factors of tasks which were needed for developing scenario to prevent intentional as well as unintentional accidents in agricultural tasks. It included field survey, usability testing of previous e-learning programs, task analysis, accident case, and risk assessments. In the final phase, the contents were developed with animation using the scenario developed in the second phase. The developed e-learning contents can be practically and easily used in educating and training older farmers for safe agricultural tasks.

Gastrointestinal endoscopy, education and accreditation in Korea

Kyung-Jo Kim(김경조),Sung Koo Lee(이성구) 대한외과학회 2014 대한외과학회 학술대회 초록집 Vol.2014 No.11

Estrogen activity of Silkworm (Bombyx mori) Pupa water extract and its fractions

Ryu, Jae-Sung,Jo, Gyeong-Jong,Jin, Jung-Woo,Yang, Hyo-Jung,Park, Yong-Il,Na, Ye-Seul,Nam, Kyung-Su,Keum, Kyung-Soo,Choo, Young-Kug Kyung Hee Oriental Medicine Research Center 2008 Oriental pharmacy and experimental medicine Vol.8 No.3

This study was conducted to evaluate the estrogen activity of silkworm (Bombyx mori) pupa extracts and their fractions. Powdered samples of freeze-dried silkworm pupa were extracted at room temperature (RT), $40^{\circ}C$, $60^{\circ}C$, $80^{\circ}C$, and $100^{\circ}C$ in water (D.W), chloroform, ethyl acetate, and methanol for 6h and then filtered (0.45 um). The extracts were then freeze-dried. The estrogenic activity of these extracts was then investigated by competition binding assays using estrogen receptor ${\alpha}\;(ER{\alpha})$ and $ER{\beta}$, and by evaluating their effects on the proliferation of the human breast cancer cell line, MCF-7. Among the extracts evaluated, water extracts prepared at RT showed the highest binding affinity to $ER{\alpha}$ ($IC_{50}$, 1.76 ug/ml) and $ER{\beta}$ ($IC_{50}$, 0.07 ug/ml). In addition, MCF-7 cells that were treated with 62.5 ug/ml of the RT extract showed the greatest increase in proliferation (2-fold; 1291.79%) when compared to control cells (659.82%). Next, the water extract that was prepared at RT (sample 1) was dissolved in D.W. and further fractionated using a Dowex 50W - 8X ($H^+$) column. The flow-through and wash were then pooled together and freeze-dried (sample 2). The bound materials were then eluted with 20 mM NaCl, after which they were applied to a Dowex 1X2 - 200 ($Cl^-$) column and washed with D.W. to remove the sodium ions. The eluants were then freeze-dried (sample 3). Of these fractions, sample 2 showed the highest binding affinity to ER{\alpha} ($IC_{50}$, 1.44 ug/ml) and $ER{\beta}$ ($IC_{50}$, 1.18 ug/ml). In addition, MCF-7 cells that were treated with sample 2 (15.6 ug/ml) showed the largest increase in growth (1159.39%) when compared to control cells (525.26%). Taken together, these results suggest that the fraction of the RT water extract of silkworm pupa referred to as sample 2 may be useful as a phytoestrogen.

Aberrant Promoter Hypomethylation of Sortilin 1: A Moyamoya Disease Biomarker

Sung, Hye Youn,Lee, Ji Yeoun,Park, Ae Kyung,Moon, Youn Joo,Jo, Inho,Park, Eun-Mi,Wang, Kyu-Chang,Phi, Ji Hoon,Ahn, Jung-Hyuck,Kim, Seung-Ki Korean Stroke Society 2018 Journal of stroke Vol.20 No.3

<P><B>Background and Purpose</B></P><P>The pathogenesis of moyamoya disease (MMD) remains poorly understood, and no reliable molecular biomarkers for MMD have been identified to date. The present study aimed to identify epigenetic biomarkers for use in the diagnosis of MMD. </P><P><B>Methods</B></P><P>We performed integrated analyses of gene expression profiles and DNA methylation profiles in endothelial colony forming cells (ECFCs) from three patients with MMD and two healthy individuals. Candidate gene mRNA expression and DNA methylation status were further validated using reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and pyrosequencing analysis of an expanded ECFC sample set from nine patients with MMD and ten controls. We evaluated the diagnostic accuracy of the potential biomarkers identified here using receiver operating characteristic curve analyses and further measured major angiogenic factor expression levels using a tube formation assay and RT-qPCR. </P><P><B>Results</B></P><P>Five candidate genes were selected via integrated analysis; all five were upregulated by hypomethylation of specific promoter CpG sites. After further validation in an expanded sample set, we identified a candidate biomarker gene, sortilin 1 (<I>SORT1</I>). DNA methylation status at a specific <I>SORT1</I> promoter CpG site in ECFCs readily distinguished patients with MMD from the normal controls with high accuracy (area under the curve 0.98, sensitivity 83.33%, specificity 100%). Furthermore, <I>SORT1</I> overexpression suppressed endothelial cell tube formation and modulated major angiogenic factor and matrix metalloproteinase-9 expression, implying <I>SORT1</I> involvement in MMD pathogenesis. </P><P><B>Conclusions</B></P><P>Our findings suggest that DNA methylation status at the <I>SORT1</I> promoter CpG site may be a potential biomarker for MMD.</P>

Analysis of gold and silver nanoparticles internalized by zebrafish (<i>Danio rerio</i>) using single particle-inductively coupled plasma-mass spectrometry

Sung, Hwa Kyung,Jo, Eunhye,Kim, Eunjeong,Yoo, Sun-kyoung,Lee, Jae-woo,Kim, Pil-je,Kim, Younghun,Eom, Ig-Chun Elsevier 2018 CHEMOSPHERE - Vol.209 No.-

<P><B>Abstract</B></P> <P>With the increase in the application of nano-consumer products containing engineered nanoparticles (NPs), the unintended environmental exposure to NPs has been inevitable. Because of the bioaccumulation of NPs, concern about their potential cytotoxicity to aquatic organisms is also growing. Although measuring tools for analyzing particle size and/or concentration of NPs in intracellular uptake of tissues have been well developed, a simultaneous analysis of the two characteristics is difficult. The objective of this study was to use single particle-inductively coupled plasma-mass spectrometry (sp-ICP-MS) to measure the bioaccumulation and particle size changes of NPs exposed to zebrafish (<I>Danio rerio</I>) for 7 days. The uptake of NPs in the liver, intestine, and gill tissues was confirmed by electron microscopic (EM) analysis. However, the primary particle size of NPs in tissues could not be determined by the EM analysis. Therefore, sp-ICP-MS coupled with alkaline digestion was used for the easy extraction and immediate analysis of NPs from tissues. Zebrafish were exposed to four NPs (30 and 80 nm gold/silver NPs; AuNPs/AgNPs). Uptake amounts of AgNPs in the liver and intestine were significantly higher than those of AuNPs. Although larger NPs were finally accumulated in the liver and intestine tissues, most of the smaller NPs were filtered in the gills. The sp-ICP-MS method coupled with alkaline digestion enabled the accurate analysis of size, size distribution, and mass concentration of NPs in an aquatic organism.</P> <P><B>Highlights</B></P> <P> <UL> <LI> SP-ICP-MS was applied to analyze the exposure and uptake of NPs by living zebrafish. </LI> <LI> High recovery efficiency of NPs from the liver of zebrafish was obtained by alkaline extraction. </LI> <LI> Uptake amounts of AgNPs in the liver and intestine were significantly higher than those of AuNPs. </LI> <LI> SP-ICP-MS enabled an accurate analysis of NP features in an aquatic organism. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>