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Chao-bin Wang,Hui Wang,Jun-shuang Zhao,Ze-jun Wu,Hao-dong Liu,Chao-jia Wang,An-rong Li,Dawei Wang,Juntao Hu 대한신경외과학회 2023 Journal of Korean neurosurgical society Vol.66 No.5
Penetrating head injury is a serious open cranial injury. In civilians, it is often caused by non-missile, low velocity flying objects that penetrate the skull through a weak cranial structure, forming intracranial foreign bodies. The intracranial foreign body can be displaced due to its special quality, shape, and location. In this paper, we report a rare case of right-to-left displacement of an airgun lead bullet after transorbital entry into the skull complicated by posttraumatic epilepsy, as a reminder to colleagues that intracranial metal foreign bodies maybe displaced intraoperatively. In addition, we have found that the presence of intracranial metallic foreign bodies may be a factor for the posttraumatic epilepsy, and their timely removal appears to be beneficial for epilepsy control.
Wang, Rong-Tyai Techno-Press 2000 Structural Engineering and Mechanics, An Int'l Jou Vol.10 No.3
The effects of weight and axial inertia of a beam are taken into account for studying the nonlinear vibration of the Timoshenko beam due to external loads. The combination of Galerkins method and Runge-Kutta method are employed to obtain the dynamic responses of the beam. A concentrated force and a two-axle vehicle traversing on the beam are taken as two examples to investigate the response characteristics of the beam. Results show that the effect of axial inertia of the beam increases the fundamental period of the beam. Further, both the dynamic deflection and the dynamic moment of the beam obtained with including the effect of axial inertia of the beam are greater than those of the beam without including that effect of the beam.
Wang, Rong-Tyai,Kuo, Nai-Yi Techno-Press 1999 Structural Engineering and Mechanics, An Int'l Jou Vol.8 No.2
The large deflection theory of the Mindlin plate and Galerkin's method are employed to examine the static responses of a plate produced by the weight of the plate, and the dynamic responses of the plate caused by the coupling effect of these static responses with a set of moving forces. Results obtained by the large deflection theory are compared with those by the small deflection theory. The results indicate that the effect of weight of the plate increases the modal frequencies of the structure. The deviations of dynamic transverse deflection and of dynamic bending moment produced by a moving concentrated force between the two theories are significant for a thin plate with a large area. Both dynamic transverse deflection and dynamic bending moment obtained by the Mindlin plate theory are greater than those by the classical plate.
Wang, Jian-Rong,Li, Yang-Yuan,Liu, Danni The Korean Society for Microbiology and Biotechnol 2015 Journal of microbiology and biotechnology Vol.25 No.6
The present study describes the gene cloning and high-level expression of an alkaline and thermostable lipase gene from Trichosporon coremiiforme V3. Nucleotide analysis revealed that this lipase gene has an open reading frame of 1,692 bp without any introns, encoding a protein of 563 amino acid residues. The lipase gene without its signal sequence was cloned into plasmid pPICZαA and overexpressed in Pichia pastoris X33. The maximum lipase activity of recombinant lipase was 5,000 U/ml, which was obtained in fed-batch cultivation after 168 h induction with methanol in a 50 L bioreactor. The purified lipase showed high temperature tolerance, and being stable at 60℃ and kept 45% enzyme activity after 1 h incubation at 70℃. The stability, effects of metal ions and other reagents were also determined. The chain length specificity of the recombinant lipase showed high activity toward triolein (C18:1) and tripalmitin (C16:0).
Rong Wang,Tao Zuo 한국해양과학기술원 2004 Ocean science journal Vol.39 No.1
The Yellow Sea Warm Current (YSWC) and the Yellow Sea Cold Bottom Water (YSCBW) are two protruding features, which have strong influence on the community structure and distribution of zooplankton in the Yellow Sea. Both of them are seasonal phenomena. In winter, strong north wind drives southward flow at the surface along both Chinese and Korean coasts, which is compensated by a northward flow along the Yellow Sea Trough. That is the YSWC. It advects warmer and saltier water from the East China Sea into the southern Yellow Sea and changes the zooplankton community structure greatly in winter. During a cruise after onset of the winter monsoon in November 2001 in the southern Yellow Sea, 71 zooplankton species were identified, among which 39 species were tropical, accounting for 54.9 %, much more than those found in summer. Many of them were typical for Kuroshio water, e.g. Eucalanus subtenuis, Rhincalanus cornutus, Pareuchaeta russelli, Lucicutia flavicornis, and Euphausia diomedeae etc. 26 species were warm-temperate accounting for 36.6% and 6 temperate 8.5%. The distribution pattern of the warm water species clearly showed the impact of the YSWC and demonstrated that the intrusion of warmer and saltier water happened beneath the surface northwards along the Yellow Sea Trough. The YSCBW is a bottom pool of the remnant Yellow Sea Winter Water resulting from summer stratification and occupy most of the deep area of the Yellow Sea. The temperature of YSCBW temperature remains ≤10oC in mid-summer. It is served as an oversummering site for many temperate species, like Calanus sinicus and Euphaisia pacifica. Calanus sinicus is a dominant copepod in the Yellow Sea and East China Sea and can be found throughout the year with the year maximum in May to June. In summer it disappears in the coastal area and in the upper layer of central area due to the high temperature and shrinks its distribution into YSCBW.
Rong Zeng,Renzhong Qiao,Zehu Wang,Hongran Wang,Liqiang Chen,Lin Yang,Liming Hu,Zelin Li 한국고분자학회 2012 Macromolecular Research Vol.20 No.4
Two kinds of chitosan-stavudine (d4T) conjugates, chitosan-O-isopropyl-5'-O-d4T monophosphate conjugate (Cs-P-d4T) with a phosphoramide linkage and chitosan-5'-O-succinyl-d4T conjugate (Cs-S-d4T) with a succinic spacer, were synthesized using an Atherton-Todd reaction and carbodiimide coupling reaction, respectively, and then structurally characterized. Their in vitro drug release behaviors and anti-human immunodeficiency virus (HIV) activity were investigated and compared. Both of the chitosan-d4T conjugates more strongly prefer to release corresponding d4T derivatives rather than free d4T in a prolonged manner but have different hydrolysis routes. The anti-HIV activity and cytotoxicity evaluated in the MT4 cell line revealed that the anti-HIV selectivity index was in the following order: Cs-P-d4T > d4T >> Cs-S-d4T since the released d4T-5'-(O-isopropyl) monophosphate from Cs-P-d4T can bypass the rate-limiting bottleneck of nucleoside phosphorylation, while the released 5'-O-succinyl-d4T from Cs-Sd4T has to be hydrolyzed to d4T and then successively phosphorylated to its active form to exert antiviral activity. The results suggested that constructing a chitosan-nucleoside reverse transcriptase inhibitor (NRTI) conjugate with a phosphoramide linkage may be an efficient approach for improving NRTI therapy efficacy in antiretroviral treatment.
Rong Zhou,Zhen Wu,Xu Wang,Eva Rosenqvist,Yinlei Wang,Tongmin Zhao,Carl‑Otto Ottosen 한국원예학회 2018 Horticulture, Environment, and Biotechnology Vol.59 No.4
Tomato cultivation at lower or higher temperatures than the optimum negatively afects plant growth and development. Large diferences in abiotic stress tolerance have been found between Solanum lycopersicum and wild tomato species. Ouraim was to compare temperature stress tolerance in cultivated and wild tomato genotypes to identify cold- and heat-toleranttomatoes for further utilization in tomato breeding. The maintained net photosynthetic rate (PN) and chlorophyll fuorescencewas related to the tolerance of tomatoes at temperature stress. The PN and chlorophyll fuorescence of one cultivated tomato(Ly from S. lycopersicum) and six wild tomatoes genotypes (Ha from Solanum habrochaites, Pe from Solanum pennellii,Pi1 and Pi2 from Solanum pimpinellifolium, Pr1 and Pr2 from Solanum peruvianum) grown at low (12 °C) and high (33 °C)temperatures were compared. The PN of four tomato genotypes during temperature stress were lower than the control, butnot in Pe, Pr1, and Pr2. The maximum quantum efciency of photosystem II (Fv/Fm) of the cultivated tomatoes was lowerat both 12 and 33 °C than the control using Handy PEA, whereas Fv/Fm using MINI-PAM was lower only at 12 °C. Thechlorophyll fuorescence OJIP transient (OJIP curve) revealed diferences between temperature stress responses and tomatogenotype. With the exception of Pr2, the Fv/Fm in wild tomatoes was unafected by temperature stress; however, they stillmaintained clear genotype diferences for other physiological traits such as PN, quantum yield of PSII (Fq′/Fm′), electrontransport rate, non-photochemical quenching, and the fraction of open PSII centers (qL). These results indicated that the wildtomato varieties Pe and Pr1 had the highest temperature stress tolerance, while the cultivated species was the more sensitiveto temperature stress in comparison. In general, the wild tomato genotypes were more tolerant to both cold and heat stressthan the cultivated tomato, suggesting that these wild species could be used to uncover underlying mechanisms of temperaturestress tolerance and will be promising sources of genetic variability for temperature stress tolerance in breeding programs.
A New Cell Counting Method to Evaluate Anti-tumor Compound Activity
Wang, Xue-Jian,Zhang, Xiu-Rong,Zhang, Lei,Li, Qing-Hua,Wang, Lin,Shi, Li-Hong,Fang, Chun-Yan Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.8
Determining cell quantity is a common problem in cytology research and anti-tumor drug development. A simple and low-cost method was developed to determine monolayer and adherent-growth cell quantities. The cell nucleus is located in the cytoplasm, and is independent. Thus, the nucleus cannot make contact even if the cell density is heavy. This phenomenon is the foundation of accurate cell-nucleus recognition. The cell nucleus is easily recognizable in images after fluorescent staining because it is independent. A one-to-one relationship exists between the nucleus and the cell; therefore, this method can be used to determine the quantity of proliferating cells. Results indicated that the activity of the histone deacetylase inhibitor Z1 was effective after this method was used. The nude-mouse xenograft model also revealed the potent anti-tumor activity of Z1. This research presents a new anti-tumor-drug evaluation method.