Usefulness of Objective Hearing Tests for Screening Patients with Partial Deafness

Yu, Min Ji,Mun, Hyung-Ah,Lee, Jong Joo,Kang, Young Sook,Hong, Soo Ah,Lim, Hye Jin,Park, Hun Yi,Choung, Yun-Hoon 한국레이저가공학회 2015 한국레이저가공학회 학술대회 논문집 Vol. No.

Molecular Cloning of Prophenoloxidase Genes from the Multicolored Asian Lady Bird Beetle, Harmonia axyridis

Min Ah Kang,Kyu Sik Yoon,Hye Ri Kwon,Min Woo Park,Eun Jin Kang,Mi Ja Seo,Yong Man Yu,Young Nam Youn 한국응용곤충학회 2010 한국응용곤충학회 학술대회논문집 Vol.2010 No.05

The multicolored Asian ladybird beetle, Harmonia axyridis, is a generalist predator of aphids also, shows a high level of phenotype polymorphism in color pattern of elytra. Although, it is not sure about genetic information of color polymorphism, it has been confirmed that this phenomenon comes from their genetic traits. The color of H. axyridis elytra is mainly composed of black and red pigment. Phenoloxidase (PO) plays an important role in many insect physiological functions, i.e. sclerotization and pigmentation of cuticle and melanization of parasites. Following activation, PO catalyses the hydroxylation of tyrosine and subsequent oxidation of phenolic substance into quinines, which are further converted to melanin. However, the molecular bases of H. axyridis color pattern formation are almost unknown but it may be that the different pro-POs have different expression. In this study, total RNA samples from four each color pattern individuals, for example, succinea 1, succinea 2, conspicua and spectabilis was extracted. A cDNA enconding pro-PO was molecular cloned from each color pattern of H. axyridis and its putative amino acid sequence shared homology with pro-PO of other insects. We are pursuing to elucidate that their pro-PO sequence will be similar with those other insect PPO sequence. There are also regions of high sequence similarity, including putative activation site and two copper binding sites.

Evaluation of two different enzyme-linked immunosorbent assay for severe fever with thrombocytopenia syndrome virus diagnosis

Yu, Min-Ah,Jeong, Hye Won,Park, Su-Jin,Kim, Young-Il,Kwon, Hyeok-Il,Kim, Eun-Ha,Si, Young-Jae,Yu, Kwang Min,Robles, Norbert John,Han, Hae Jung,Choi, Young Ki 대한백신학회 2018 Clinical and Experimental Vaccine Research Vol.6 No.1

<P>To develop the large scale serological assay for severe fever with thrombocytopenia syndrome virus (SFTSV) infection, we evaluated two different enzyme-linked immunosorbent assay (ELISA) methods using nucleocapsid protein (NP) and Gn proteins of CB1 (genotype B) SFTSV strains. The NP-based ELISA tests showed more sensitive with broad cross-reactivity between two different genotype A and B strains compared with those of Gn-based ELISA tests. However, Gn-based ELISA showed more genotype specificity and specificity. These result suggested that NP-based ELISA test could be applicable for general sero-prevalence studies of SFTSV infections, while Gn-based ELISA could be applicable for a certain specific genotype sero-prevalence study.</P>

Paricalcitol Pretreatment Attenuates Renal Ischemia-Reperfusion Injury via Prostaglandin E ₂ Receptor EP4 Pathway

Hong, Yu Ah,Yang, Keum Jin,Jung, So Young,Park, Ki Cheol,Choi, Hyunsu,Oh, Jeong Min,Lee, Sang Ju,Chang, Yoon Kyung,Park, Cheol Whee,Yang, Chul Woo,Kim, Suk Young,Hwang, Hyeon Seok Hindawi 2017 Oxidative medicine and cellular longevity Vol.2017 No.-

<P>The protective mechanism of paricalcitol remains unclear in renal ischemia-reperfusion (IR) injury. We investigated the renoprotective effects of paricalcitol in IR injury through the prostaglandin E<SUB>2</SUB> (PGE<SUB>2</SUB>) receptor EP4. Paricalcitol was injected into IR-exposed HK-2 cells and mice subjected to bilateral kidney ischemia for 23 min and reperfusion for 24 hr. Paricalcitol prevented IR-induced cell death and EP4 antagonist cotreatment offset these protective effects. Paricalcitol increased phosphorylation of Akt and cyclic AMP responsive element binding protein (CREB) and suppressed nuclear factor-<I>κ</I>B (NF-<I>κ</I>B) in IR-exposed cells and cotreatment of EP4 antagonist or EP4 small interfering RNA blunted these signals. In vivo studies showed that paricalcitol improved renal dysfunction and tubular necrosis after IR injury and cotreatment with EP4 antagonist inhibited the protective effects of paricalcitol. Phosphorylation of Akt was increased and nuclear translocation of p65 NF-<I>κ</I>B was decreased in paricalcitol-treated mice with IR injury, which was reversed by EP4 blockade. Paricalcitol decreased oxidative stress and apoptosis in renal IR injury. Paricalcitol also attenuated the infiltration of inflammatory cells and production of proinflammatory cytokines after IR injury. EP4 antagonist abolished these antioxidant, anti-inflammatory, and antiapoptotic effects. The EP4 plays a pivotal role in the protective effects of paricalcitol in renal IR injury.</P>

Imidazole Trimer-Water Complexes in Superfluid Helium Nanodroplets: Water Stretching Modes

Ahn, Ah-Reum,Lee, Seung-Jun,Lee, Seul-Ki,Min, Ah-Reum,Kim, Yu-Sic,Jung, Hyeon-Jin,Hong, Seong-Min,Lee, Joon-Hwa,Choi, Myong-Yong,Miller, Roger E. Korean Chemical Society 2011 Bulletin of the Korean Chemical Society Vol.32 No.4

만5세 일반아동과 기능적 조음음운장애아동의 음운처리능력과 읽기 능력 비교

유민아 ( Yu Min-Ah ),임영심 ( Lim Young-sim ),김은나 ( Kim Eun-na ) 한국유아교육학회 2021 유아교육연구 Vol.41 No.6

기능적 조음음운장애아동과 일반아동에 비해 음운인식능력과 읽기 능력이 주장은 있으나, 음운적 작업기억과 음운적 정보회상을 포함한 음운처리 능력 전체와 읽기능력간의 상관관계를 살펴보는 선행 연구는 부족하다. 따라서 본 연구에서는 만 5세 일반아동과 기능적 조음음운장애 아동의 음운처리능력과 읽기 능력을 살펴보고 각 집단에서 음운처리능력 하위영역 간의 상관관계를 살펴보고자 하였다. 본 연구의 대상은 만 5세 기능적 조음음운장애 아동 20명과 일반 아동 20명으로 총 40명으로 선정하였다. 음운인식 능력은 음절 수준으로 측정하였으며, 음운적 작업기억 능력은 숫자 회상 검사와 무의미 단어 회상 검사로 측정하였고, 음운적 정보회상 능력은 사물 빠르게 말하기 과제와 색깔 빠르게 말하기 과제를 사용하였다. 읽기 능력 측정을 위해서 고빈도, 저빈도, 무의미로 나눠 단어재인과 읽기 유창성 능력을 측정하였다. 연구결과 집단간 음운처리 능력 차이에서 일반아동이 기능적 조음음운장애 아동보다 음운처리능력과 단어 인지능력에서 높게 나타났다. 일반아동과 기능적 조음음운장애아동은 음운인식능력은 단어인지와 읽기 유창성 모두 상관관계가 나타났다. 그러나 일반아동은 음운적정보회상능력이 단어인지 능력과 상관관계를 보였고, 기능적 조음음운장애아동 음운적 정보회상능력이 음운인식과 음운적작업기억능력과 상관관계를 나타내었다는 점에서 차이가 있다. 이는 기능적 조음음운장애 아동은 각각의 음운처리가 일반아동에 비해 빠르게 잘 이뤄지지 않다는 것을 나타낸다. 따라서, 기능적 조음음운장애아동은 읽기 단계 전 말소리 훈련과 같은 음운처리 훈련이 필수적으로 이뤄져야 함을 시사한다. This research was performed to compare the phonological processing and reading ability characteristics between the children with and without functional articulatory and phonological disorders. The two groups’ differences were observed in terms of phonological processing and reading abilities, along with the correlation and impacts present between the two characteristics. The research was conducted to 20 children without disabilities and 20 children with functional articulatory and phonological disorders, both in age 5. The summary of result is as follows. First, the children without disabilities in age 5 showed better phonological processing ability than the children with functional articulatory and phonological disorders did. Second, the children without disabilities in age 5 presented greater reading ability than the children with functional articulatory and phonological disorders did. Third, the difference in the reading ability between children with and without functional articulatory and phonological disorders in age 5 was only found in word recognition. The phonological awareness of children with and without functional articulatory and phonological disorders affected both their abilities of word recognition and reading for fluency. Furthermore, such a result was more explainable in the case of children with functional articulatory and phonological disorders. In both groups, the greater word recognition ability was correlated with higher reading for fluency ability. In the case of children without disabilities, their phonological awareness was only correlated with their reading ability. On the other hand, the phonological awareness of children with functional articulatory and phonological disorders had a positive relationship with their phonological information retrieval, word recognition, and reading for fluency.

Targeted Bile Acid Metabolite Analysis Related to Obesity in Human Feces

Su Min Kim,Jin Ah Won,Jun Sang Yu,So Young Jo,Min Ho Kim,Ho Young Park,Yu Ra Lee,Hye Hyun Yoo 한국분석과학회 2023 학술대회논문집 Vol.2023 No.11

Insectisidal activity and feeding behavior of green peach aphid, Myzus persicae against bio-nano type of Pyrifluqinazon

Kang, Min Ah,Kyu Sik Yoon,Hye Ri Kwon,Min Woo Park,Shin Hyuk Jo,Shin Hyo Seob,Sae Hee Kim,Eun Jin Kang,Mi Ja Seo,Yong Man Yu,Young Nam Youn 한국응용곤충학회 2010 한국응용곤충학회 학술대회논문집 Vol.2010 No.10

Pyrifluquinazon, as a quinazinalone chemical group, based on a new mode of biological activity. It is reported that mode of action is modifies insect behavior, rapidly stopping feeding such that insects starve to death. Time-release feature and mortality effect on M. persicae using different pyrifluquinazon nano type and non-nano type were compared. Pyrifluquinazon nano type was formulated with different molecular weight and density of used chitosan (CS 30000 0.1% and CS 3000 0.3%). In the CS 30,000 0.1%, the mortality was weakly occurred at early time, but steadily increased after 4days. Finally, we confirmed more than 70% mortality as a peak at 16days. In CS 3000 0.3%, the mortality showed about 70% until 18days as a effective controlled release. Also, We examine time-release feature and mortality effect on M. persicae according to the different pyrifluquinazon nano type(CS 30000 0.1% and CS 3000 0.3%) of concentrations. The CS 30000 0.1% bioassay results of different concentration were showed that the highest concentration(100ppm) was measured better mortality than other concentration at 0 day, but cannot confirm different effect about dissimilar concentration. However, increasing rates of M. persicae were low as treatment concentrate was high. In CS 3000 0.3% 100ppm concentration bioassay result, aphid mortality reached peak at 24 days and increasing rate also low. Additionally, for the comparing of bioassay and feeding behavior of M. persicae against pyrifluquinazon nano types and non-nano type, EPG technique was carried out. In case of non nano type, feeding inhibition efficacy was showed during 4 days after treatment, but appeared similar level with control after 10days. In CS 3000 0.3% 50ppm, residual efficacy was specially showed until 28days after treatment whereas treatments with CS 30000 0.1% were similar to the control after 22days. These result show that the change of feedinng behavior and motrality of M. persicae is correlated with the change of nano type or non nano type of pyrifluquinazon.

The determination of dark adaptation time using electroretinography in conscious Miniature Schnauzer dogs

서강문,김세은,Je-Min Chae,이나영,Hyung-Ah Yu,정만복,박신애,김원태 대한수의학회 2007 Journal of Veterinary Science Vol.8 No.4

The optimal dark adaptation time of electroretinograms (ERG's) performed on conscious dogs were determined using a commercially available ERG unit with a contact lens electrode and a built-in light source (LED-electrode). The ERG recordings were performed on nine healthy Miniature Schnauzer dogs. The bilateral ERG's at seven different dark adaptation times at an intensity of 2.5 cdㆍs/m² was performed. Signal averaging (4 flashes of light stimuli) was adopted to reduce electrophysiologic noise. As the dark adaptation time increased, a significant increase in the mean a-wave amplitudes was observed in comparison to base-line levels up to 10 min (p < 0.05). Thereafter, no significant differences in amplitude occured over the dark adaptation time. Moreover, at this time the mean amplitude was 60.30 ± 18.47 μV. However, no significant changes were observed for the implicit times of the a-wave. The implicit times and amplitude of the b-wave increased significantly up to 20 min of dark adaptation (p < 0.05). Beyond this time, the mean b-wave amplitudes was 132.92 ± 17.79 μV. The results of the present study demonstrate that, the optimal dark adaptation time when performing ERG's, should be at least 20 min in conscious Miniature Schnauzer dogs.

External Morphology and Distribution of Antennal Sensilla of Aphidius colemani

Hye Ri. Kwon,Min Ah Kang,Kyu Sik Yoon,Min Woo Park,Eun Jin Kang,Mi Ja Seo,Yong Man Yu,Young Nam Youn 한국응용곤충학회 2010 한국응용곤충학회 학술대회논문집 Vol.2010 No.05

Several species of the genus Aphidius are used in biological control programs against aphid pests throughout the world and their behavior and physiology are well studied. But despite knowing the importance of sensory organs in their behavior, their antennal structure is largely unknown. In this study, the external morphology and distribution of the antennal sensilla on the antennal of both female and male adults of A. colemani were described using scanning electron microscopy (SEM). Generally, the filaform antennae of males (1,565.60± 194.64㎛) are longer than females (1,303.83±156.41㎛). Antennae of this species is made up of scape, pedicel and flagellomeres. Male and female antennae differed in the total number of flagellomeres as 15 in males and 13 in females. Female and male antennae of A. colemani has samely ten types of sensilla. We classified 3 types of sensilla trichodea as without pores, with a tip pore and with wall pores, 3 types of sensilla coeloconica, 1 sensilla placodea and 1 Bohm bristles. In addition, the possible functions of the above sensilla types are discussed in light of previously published literature; mechanoreception (Bohm bristles and sensilla coeloconica) and olfaction (sensilla trichodea and sensilla placodea). Future studies on the functional morphology of the antennal sensilla of A. colemani using transmission electron microscopy (TEM) coupled with electrophysiological recordings will likely confirm the functions of the different sensilla identified in this study.