환자만족도 조사설문지의 설문문항 배열에 따른 신뢰도 분석

최귀선,조우현,홍재석,이선희,강명근 한국의료QA학회 2000 한국의료질향상학회지 Vol.7 No.2

Objectives : The purpose of this study was to evaluate the reliability of questionnaire according to item arrangement on patient satisfaction questionnaire. Methods : We developed the two types of questionnaire with different item arrangement. In the first type (A), questions were arranged according to medical service dimensions. Questions in the second type (B) were arranged according to medical process. Both questionnaires were composed of six dimensions: physical environments, process, competence, courtesy, information giving, understanding patients. Measurements were performed on a 5-score Likert scale. In an outpatients and inpatient survey, total 777 patients answered the type A (outpatients: 257, inpatients: 128) or Type B (outpatients: 257, inpatients: 135). In order to compare the internal consistency of two types. Cronbach's α were calculated. Multiple regression analysis was also performed to know which type of questionnaire explain more of the overall satisfaction. Results : In outpatient survey, type A questionnaire showed higher internal consistency than B except physical environments dimension. Also in inpatient survey, type A had higer internal consistency than B in four dimensions(process, competence, courtesy, understanding patients). In the results of multiple regression analysis, type A questionnaire (R² =0.53) explained more of the variation in overall satisfaction then B questionnaire (R2=0.43) in outpatient survey. In inpatient survey, type B questionnaire (R²=0.40) explained, more of the variation in overall satisfaction than type A questionnaire (R² =0.33).But the.difference of R was not significant in inpatient survey. Conclusion : The results of this study support that type A questionnaire has higer reliability in assessment of consumer satisfaction than type B.

압력비에 따른 2차원 초음속 터빈의 유동특성에 대한 실험적 연구

조종재,김귀순,김진한,이은석 朝鮮大學校 機械技術硏究所 2005 機械技術硏究 Vol.8 No.1

본 연구에서는 초음속 충동형 터빈의 유동특성을 알아보기 위해 소형 초음속 풍동을 설계 하였으며 Single pass Schlieren system을 이용하여 유통을 가시화하였다. 실험은 2차원 초음속 노즐과 익렬을 조합하여 압력비에 따라 실시하였다. 실험을 통해 충격파를 포함한 복잡한 유동 형태와 노즐-익렬, 충격파-경계층 상호작용 등을 관찰할 수 있었다. In this paper, a small supersonic wind tunnel is designed and built to study the flow characteristics of a supersonic impulse turbine. The flow is visualized by means of a single pass Schlieren system. The supersonic cascade with 2-dimensional supersonic nozzle was tested over a wide range of pressure ratio. Highly complicated flow patterns including shocks, nozzle-cascade interaction and shock boundary layer interactions are observed.

감나무탄저병에서 분리한 Gloeosporium kaki의 2계통

崔貞植,李貴宰,金炯武,蘇仁永 全北大學校 1993 論文集 Vol.35 No.-

A anthracnose symptom at persimmon tree was found out in the field, Chonju in Chonbuk province and Haenam Experiment Station in Chonnam province in 1991 and 1992. The cultivated character and the pathogenicity of the fungi were as follows : These fungi, Gloeosparium kaki which were isolated from the anthracnose were classified into strain Ⅰ and Ⅱ by the cultivated characteristics. The size of conidia in strain Ⅰ was 22.5 × 6.82 ㎛ and that of strain Ⅱ was 20.3 × 6.2 ㎛. In a PDA, the colour of strain I was light brown and made a central zone. The colour of strain Ⅱ was black brown and deep grey and didn't make a central zone. The pathogenicity of strain Ⅰ was similar to strain Ⅱ in the leaf, branches.

Capsid Protein Gene-Mediated Resistance to Barely Yellow Mosaic Virus and Barely Mild Mosaic Virus in Transgenic Barely

Lee Kui-Jae,Park Min-Kyung,Rha Eui-Sik,Lee Wang-Hyu 한국자원식물학회 2003 한국자원식물학회 학술심포지엄 Vol.2003 No.-

Leaf discs and apical meristems were cultured in Murashige and Skoog (MS) medium supplemented with cytokinin and auxin at different concentrations. Callus production was observed in all tested media after six days of incubation. Callus produced in the presence of high concentration of NAA (2.0mg/1) was fragile in texture and yellow in colour. Highest callus formation was observed from leaf discs in the medium supplemented with 1.0mg/1 NAA and 0.5 mg/l BAP in dark at <TEX>$25{\pm}1{\circ}C$</TEX>. Percentage of callus formation was 95% and mean callus fresh weight was 654.88 43.53 mg. Shoots were induced from the callus after 4 weeks in 1/2MS medium supplemented with BAP and kinetin both at 0.5mg/1. When elongated shoots were separated and transferred into multiplication medium (MS+0.5mg/1 BAP+0.5mg/1 kinetin) multiplication rate was 6.4 after 6 weeks. Higher concentrations of BAP caused callus production at the base. Direct shoot induction was observed from apical meristems in MS medium in the presence of 0.175 mg/1 IAA + 2.25mg/1 BAP and 0.175 mg/1 IAA + 3.0 mg/1 BAP in 16 hour day at <TEX>$25{\pm}1{\circ}C$</TEX>. Explants (apical meristems) elongated to form a single shoot forming a callus at the base. Adventitious buds were sprouted out from the base. Percentage explants which producing shoots was 28.57 and 65.5 respectively. Multiple shoot induction was also observed in the same media. Highest multiple shoot production was observed in the presence of 0.175 mg/l IAA and 3.0mg/l BAP, Mean number of shoots per explant was 5.36 and the mean shoot length was <TEX>$16.66{\pm}4.15$</TEX>mm. Shoots (20 30m length) were tested for root induction. Excised shoots were transferred into rooting media, which contains different concentrations of NAA and IAA. Best rooting performance was observed in 1/2MS medium supplemented with 0.1mg/1 NAA after 10 days of incubation in 16 hr photoperiod at <TEX>$25{\pm}1{\circ}C$</TEX>. Mean number of roots per shoot was 6 and the mean ro

Distribution and Isolation of Soil borne Wheat Mosaic Virus in Korea

Kui-Jae Lee,Hyun-Suk Lim,Hyung-Moo Kim,Wang Hyu Lee 한국자원식물학회 2001 Plant Resources Vol.4 No.1

This study was conducted to investigate the occurrence of Soil borne wheat mosaic virus(SbWMV) in barley fields in Korea and to examine the host pathogenicity of SbWMV. By using the ELISA test, SbWMV was detected in the six regions : Suwon, Milyang, Jinju, Youngkwang, Iksan, and Chonju. SbWMV was isolated from the two strains, Albori strain from Jinju and Eunpamil strain from Milyang. SbWMV was collected from leaves showing mosaic, yellowing and necrosis stripes. SbWMV was inoculated mechanically on 1~1.5 leaf stages with leaf-rubbing to identify the host pathogenicity of 36 Korean barley cultivars, a wheat cultivar, two rye cultivars, three Japanese barley cultivars and Chenopodium amaranticola. Viral sympoms of inoculated leaves appeared on inoulted leaves about 4 to 6 weeks of inoculation. Baegdong and Tapgolbori, infected from Albori strain and Eunpamil strain infected from Samdobori showed much higher susceptibility than C. amaranticola and C. quinoa which showed ring spots and chlorotic spots respectively. Virus particles were observed by the electron microscope. They were rod-shapes, which are bipartite, of 142 nm or 281 nm in length with 20 nm diameter on infected leaves. Specific detection and identification of SbWMV was set up using the RT-PCR. PCR fragments of SbWMV(0.5kb) were obtained by using the designed primers for SbWMV RNA 2.

Comparison and Sequence Analysis of the 3 -terminal Regions of RNA 1 of Barley Yellow Mosaic Virus

Kui Jae Lee,Satoshi Kashiwazaki 한국자원식물학회 1998 Plant Resources Vol.1 No.2

An isolate of barley yellow mosaic virus(BaYMV-HN) obtained from Haenam, Korea was compared with two BaYMV strains, BaYMV- 1 -1 from Japan and BaYMV-G from Germany. The sequence of the 3 terminal 3817nucleotides(excluding the poly (A) tail] of RNA I of BaYMV-HN was determined to start within a long open reading frame coding for a part of the Nia-VPg polymerase(26 amino acids), Nla-Pro polymerase (343 amino acids), NIb polymerase(528 amino acids) and the entire capsid protein(297 amino acids), which is followed by a noncoding region(NCR) of 235 nucelotides. In the partial ORFs, BaYMV-HN shows higher sequence homology with BaYMV- I -1(99.5%) than BaYMV-G(92.7%). The 3 non-coding regions of BaYMV HN(235nt) shows higher nucleotide sequence homology with BaYMV-G(235nt) (99.6%) than BaYMV- I - 1(231nt) (97.0%). The 3 Nla-Pro protein sequence of BaYMV-HN shows higher amino acid sequence homology with BaYMV- I -1(95.0%) than BaYMV-G 93.6%), but, Nlb protein sequence of BaYMV-HN shows same all amino acid sequence. The capsid protein sequence of BaYMV-HN(297aa) shows same with BaYMV- I - 1, and shows higher nucleotide sequence homology with BaYMV-UK (from United Kingdom) (97,3%) than BaYMV-G(96.9%) and G2(96.9%). Difference of capsid protein amino acid were 0-9 between the Japan, United Kingdom and Germany and were 2-6 between all Korean isolates. Many of the amino acid differences are located in the N-terminal regions of the capsid proteins from 1 to 74 amino acid positions.

Effectiveness of Interventions to Increase the Participation Rate of Gastric Cancer Screening in the Republic of Korea: a Pilot study

Lee, Myung-Ha,Lee, Yoon-Young,Jung, Da-Won,Park, Bo-Young,Yun, E-Hwa,Lee, Hoo-Yeon,Jun, Jae-Kwan,Choi, Kui-Son Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.3

This study assessed the effectiveness of three intervention strategies to improve the participation rate of gastric cancer screening among people who had never undergone such screening, and those who had been screened for the disease, but not recently. It was conducted in the Ilsandong-gu District of Goyang City, Korea. The population for the current study was restricted to male residents, aged 40-65 years, who received an invitation letter to undergo gastric cancer screening from the National Health Insurance (NHI) Corporation at the beginning of 2010. The subjects were divided into two categories according to their screening history: never-screened, and ever-screened. A total of 2,065 men were eligible: 803 never-screened and 1,262 ever-screened. In each screening category they were randomly assigned to one of three intervention groups: 1) tailored telephone counseling; 2) tailored postcard reminder after tailored telephone counseling;and 3) tailored telephone counseling after tailored postcard reminder. At 3 months post-intervention, never-screened men with any intervention were more likely to undergo gastric cancer screening (OR=2.75, 95% CI: 1.22-6.18) compared to those in the reference group (no intervention). However, there was no statistically significant intervention effect in ever-screened men (OR=1.21, 95% CI: 0.65-2.27). Examination of the intervention effects by intervention group among never-screened men showed that those in the postcard reminder after telephone counseling group to be statistically significantly more likely to undergo gastric cancer screening (OR=4.49, 95% CI: 1.79-11.29) than the reference group (no intervention). Our results highlight that use of tailored postcard reminders after tailored telephone counseling is an effective method to increase participation in gastric cancer screening among men who had never been screened.

Morphology and Molecular Phylogeny of Pseudocercospora fatouae Associated with Hypophyllous Leaf Mold on Fatoua villosa in Korea

Lee Kui-Jae,Choi In-Young,Abasova Lamiya,Choi Joon-Ho,Park Jung-Hee,Shin Hyeon-Dong 한국균학회 2023 韓國菌學會誌 Vol.51 No.2

Dark brown leaf molds growing in a vein-limited pattern have been continuously observed on the lower leaf surface of Fatoua villosa in Korea. Fifteen samples deposited in the Korea University herbarium were examined for fungal morphological characterization. Two monoconidial isolates were obtained in 2022, and molecular analysis was performed based on a combined multigene dataset of the internal transcribed spacer (ITS) region and partial actin (actA), translation elongation factor 1-alpha (tef1), and DNA-directed RNA polymerase II second-largest subunit (rpb2). Based on morphological features and molecular phylogenetic analysis, the fungus Pseudocercospora fatouae was identified. To the best of our knowledge, it is the first study reporting the presence of P. fatouae on F. villosa in Korea.

Complete Genome of Methicillin-Resistant Staphylococcus epidermidis Z0118SE0272 Isolated from a Residential Environment

Lee Haeseong,Oh Jae-Young,Lee Kui Jae,Chae Jong-Chan 한국미생물·생명공학회 2023 한국미생물·생명공학회지 Vol.51 No.4

Staphylococcus epidermidis is a normal flora of human skin and is occasionally associated with pathogenic infections. We report the complete genome sequence of methicillin-resistant Staphylococcus epidermidis strain Z0118SE0272 isolated from the residential environment sharing by a companion dog and dwellers. Resistance to cefoxitin was observed in the strain, whereas it was susceptible to erythromycin, clindamycin, quinupristin-dalfopristin, trimethoprim-sulfamethoxazole, mupirocin, vancomycin, teicoplanin, linezolid, and tigecycline. The strain Z0118SE0272 identified as sequence type 130 possessed the mecA gene responsible for methicillin resistance, which composed the new type of staphylococcal cassette chromosome mec elements lacking mecRI.

High frequency plant regeneration from mature embryos of an elite barley cultivar (Hordeum vulgare L. cv Baegdong)

Lee Kui-Jae,Wtpsk Senarath,Lee Wang-Hyu 한국자원식물학회 2003 한국자원식물학회 학술심포지엄 Vol.2003 No.-