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Kang, Kee Ryeon,Kim, Yeon Sook,Wolff, Edith C,Park, Myung Hee American Society for Biochemistry and Molecular Bi 2007 The Journal of biological chemistry Vol.282 No.11
<P>Deoxyhypusine hydroxylase (DOHH) is a novel metalloenzyme that catalyzes the final step of the post-translational synthesis of hypusine (Nepsilon-(4-amino-2-hydroxybutyl)lysine) in the eukaryotic translation initiation factor 5A (eIF5A). Hypusine synthesis is unique in that it occurs in only one protein, denoting the strict specificity of the modification enzymes toward the substrate protein. The specificity of the interaction between eIF5A and DOHH was investigated using human eIF5A (eIF5A-1 isoform) and human recombinant DOHH. DOHH displayed a strong preference for binding the deoxyhypusine-containing form of eIF5A, over the eIF5A precursor or the hypusine-containing eIF5A, indicating a role for the deoxyhypusine residue in binding. In addition to the deoxyhypusine residue, a large portion of the eIF5A polypeptide (>20-90 amino acids) is required for effective modification by DOHH. We have identified the amino acid residues of DOHH that are critical for substrate binding by alanine substitution of 36 conserved amino acid residues. Of these, alanine substitution at Glu57, Glu90, Glu208, Glu241, Gly63, or Gly214 caused a severe impairment in eIF5A(Dhp) binding, with a complete loss of binding and activity in the E57A and E208A mutant enzymes. Only aspartate substitution mutants, E57D or E208D, retained partial activity and substrate binding, whereas alanine, glutamine, or asparagine mutants did not. These findings support a proposed model of DOHH-eIF5A binding in which the amino group(s) of the deoxyhypusine side chain of the substrate is primarily anchored by gamma-carboxyl groups of Glu57 and Glu208 at the DOHH active site.</P>
Studies on Anti-inflammatory and Anti-aging activity of Fermented Ligustrum Fructus
Su Ryeon CHOI,Eun Jin SHIN,Kee-Young LEE,Sang Moon KANG,Mi Kyeong LEE,Hyung Seo HWANG 한국생물공학회 2021 한국생물공학회 학술대회 Vol.2021 No.10
The dried fruit of Ligustrum lucidum, Ligustici Fructus, is known to have anti-cancer and anti-inflammatory properties as well as skin improvement. Phytochemical studies of L. lucidum fruit have resulted in the isolation of novel active compounds and the identification of their roles in antioxidant and anti-inflammatory activities. To obtain a large amount of active ingredients, Ligustici Fructus extract was fermented under various conditions using three types of microorganisms: Galactomyces Reesii, Bacillus methylotrophicus, and Saccharomyces cerevisiae. A total of 16 fermented extracts were obtained and effective fermented extracts were screened through DPPH/ABTS radical scavenging analysis and nitric oxide analysis. Among them, E-BF-WB (80% EtOH extract of Ligustici Fructus fermented without broth by Bacillus methylotrophicus) showed the highest activity. First, E-BF-WB significantly inhibited the expression of iNOS, COX-2 gene and inflammatory cytokines IL-1α/β/-6 and TNF-α in LPS-induced RAW264.7 marcophage cells. In addition, MMP-1/2/3/9 involved in wrinkling was significantly reduced in TNF-α-induced Hs68 fibroblasts by E-BF-WB. From these results, we confirmed that E-BF-WB has antioxidant, anti-inflammatory and skin wrinkle improvement functions. These results suggest that E-BF-WB will be developed as a new bio-cosmetic material for skin soothing and anti-aging cosmetics in the future.
Long-term outcomes of abdominal paraganglioma
Hye Ryeon Choi,Zeng Yap,Soon Min Choi,Sun Hyung Choi,Jin Kyong Kim,Cho Rok Lee,Jandee Lee,Jong Ju Jeong,Kee-Hyun Nam,Woong Youn Chung,Sang-Wook Kang 대한외과학회 2020 Annals of Surgical Treatment and Research(ASRT) Vol.99 No.6
Purpose: Paragangliomas (PGL) are rare neuroendocrine tumors derived from chromaffin cells of the autonomic nervous system. We aim to describe our experience and the long-term outcome of abdominal PGL over the last decade. Methods: A retrospective review of patients diagnosed with PGL in our hospital between November 2005 and June 2017 was conducted. All nonabdominal PGL were excluded and the clinicopathological features and long-term outcomes of the patients were analyzed. Results: A total of 46 patients were diagnosed with abdominal PGL. The average age of diagnosis was 55.4 years and there was no sex predilection. The average tumor size was 5.85 cm and they were predominantly located in the infrarenal position (50%). The mean follow-up period was 42 months (range, 1.8–252 months). All patients with metastases had Pheochromocytoma of the Adrenal Gland Scaled Score (PASS) of ≥4. One patient presented with synchronous metastases while 2 developed local recurrence and distant metastases. One presented with only local recurrence. One patient died 5 years after diagnosis. Conclusion: Abdominal PGL is a rare tumor with excellent long-term prognosis. Recurrence although uncommon, can occur decades after initial diagnosis. Long-term follow-up is therefore recommended for all patients with PGL, especially in patients with PASS of ≥4
Choi Hye Ryeon,Choi Sun Hyung,Hong Namki,Rhee Yumie,Kim Jin Kyong,Lee Cho Rok,Kang Sang-Wook,Lee Jandee,Jeong Jong Ju,Nam Kee-Hyun,Chung Woong Youn 대한의학회 2022 Journal of Korean medical science Vol.37 No.13
Background: Normocalcemic primary hyperparathyroidism (NPHPT) was first described in 2008. It is defined as consistently elevated serum parathyroid hormone (PTH) levels with normal serum calcium (sCa) concentration, after excluding secondary causes of PTH elevation. However, the exact definition and management strategy for NPHPT remain controversial. We retrospectively investigated the clinicopathological features and short-term outcomes of NPHPT patients. Methods: A total of 280 patients who were surgically indicated for primary hyperparathyroidism (PHPT) at the Yonsei Severance Medical Center between 2015 and 2019 were included. Patients were classified according to preoperative PTH, corrected sCa, and ionized calcium (iCa) levels as follows: typical primary hyperparathyroidism (TPHPT, elevated PTH, sCa, and iCa, n = 158) and NPHPT (elevated PTH, normal sCa, n = 122). Results: NPHPT was commonly seen in younger individuals (aged < 50 years, P = 0.025); nephrolithiasis and bone fractures were common. Preoperative PTH level was higher in the TPHPT group (P < 0.001). The NPHPT group had higher numbers of multiple parathyroid lesions (P = 0.004) that were smaller (P = 0.011). NPHPT patients were further divided into two subgroups according to iCa levels: the elevated (n = 95) and normal iCa (n = 27) groups. There was no significant difference between the two subgroups regarding symptoms and multiplicity of lesions. Conclusion: We found that NPHPT may be a heterogeneous disease entity of PHPT with high rates of multi-gland disease, which appears to be biochemically milder but symptomatic. Intraoperative PTH monitoring might help increase the surgery success rate. Moreover, the short-term outcomes of NPHPT after surgery did not differ from that of TPHPT.
Proteomic Analysis of Rat Brains Following Exposure to Electroconvulsive Therapy
Lee, Cheol Soon,Kang, Kee Ryeon,Lee, Ji-Young,Park, Chul Soo,Hahn, Kyu Hee,Sohn, Jin Wook,Kim, Bong Jo The Korean Academy of Medical Sciences 2009 JOURNAL OF KOREAN MEDICAL SCIENCE Vol.24 No.1
<P>Electroconvulsive therapy (ECT) is one of the most effective treatments used in psychiatry to date. The mechanisms of ECT action, however, are the least understood and still unclear. As a tool to elucidate the mechanisms of action of ECT, we employed proteomic analysis based on the identification of differentially expressed proteins after exposure to repeated ECT in rat brains. The expression of proteins was visualized by silver stain after two-dimensional gel electrophoresis. Of 24 differentially expressed protein spots (<I>p</I><0.05 by Student t-test), six different proteins from 7 spots were identified by matrix-assisted laser desorption/ionization time-of flight (MALDI-TOF)/mass spectrometry. Among the identified proteins, there were five dominantly expressed proteins in the ECT-treated rat brain tissues (<I>p</I><0.05); S100 protein beta chain, 14-3-3 protein zeta/delta, similar to ubiquitin-like 1 (sentrin) activating enzyme subunit 1, suppressor of G2 allele of SKP1 homolog, and phosphatidylinositol transfer protein alpha. The expression of only one protein, ACY1 protein, was repressed (<I>p</I><0.05). These findings likely serve for a better understanding of mechanisms involved in the therapeutic effects of ECT.</P>
Kim, Choong Won,Kim, Yeon Woong,Kang, Kee Ryeon,Eom, Mi-Ok,Jung, Eun Joo,Kim, Jong Chul,Ahn, Hong Joon,Kang, Yoon-Se 경상대학교 유전공학연구소 1992 遺傳工學硏究所報 Vol.11 No.-
Chicken elongation factor 2(EF-2) cDNA was isolated from a chicken intestine cDNA libary and the sequence was determined. The probe for the screening of library was prepared by polymerase chain reaction(PCR) using two degenerate oligonuckeotide primers encoding NH_2-terminal region (residue 9-14) of chicken EF-2 and a conserved GTP binding domain (residue 80-84) of hamster EF-2. The sequence of amplified 228 bp product was identical to that of hamster EF-2(residue 9-84). One of 5 positive clones, pCEK4, consisted of 3,144 nucleotides with 2,574 bp open reading frame coding for 858 amino acid reaidues. The M_r 95,361 of the protein calculated from the amino acid sequence agreed well with the molecular weight of chicken and other mammalian EF-2(95,000) measured by SDS-PAGE. Sequence identity between chicken and other mammalian EF-2s(human, hamster, and rat) was 97%, while the sequence of GTP-binding/-hydrolysis domain was 99% identical. This result indicates that EF-2 is one of the highly conserved proteins and its structure has not been changed significantly during evolution. When expression of EF-2 in various stage of chicken embryo development was estimated by measuring mRNA and protein of EF-2, the result showed that the levels of its EF-2 mRNA and protein levels were the highest in 3 day embryo and then decreased gradually.