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Electropulsing Treatment on Enhancement of Electrical Conductivity of Screen-Printed Ag Wire
Ju‑Won Park,Howook Choi,Hwangsun Kim,Simoon Sung,Hye‑Jin Jeong,Il Kim,Jaeseok Gong,Sung‑Tae Hong,Heung Nam Han 대한금속·재료학회 2021 METALS AND MATERIALS International Vol.27 No.5
The effect of high electric current density on the sintering of Ag wires manufactured by screen printing is evaluated throughelectrical resistivity analysis and microstructure observation. Different forms (continuous and pulsed) of electric currentwith different current densities are applied to the specimens. Conventional heat treatment is also performed as a controlgroup to examine the athermal effect of electropulsing treatment. Compared to the conventional heat treatment, the resistivityis reduced more under the electropulsing treatment with continuous current for the same temperature and treatment time.Also, the process time of electropulsing treatment can be reduced by applying a pulse form of high density current insteadof continuous current without losing the benefit of enhanced reduction of resistivity. The microstructural observationsobtained from high angle annular dark field scanning transmission electron microscope and a digital precession instrumentclearly show that necking connecting the crystals is formed more firmly under electric current. In addition, the temperaturechange of Ag wire and substrate is calculated according to the change of the resistivity when the electric current is appliedto confirm the reliability.
TIARA: a database for accurate analysis of multiple personal genomes based on cross-technology
Hong, Dongwan,Park, Sung-Soo,Ju, Young Seok,Kim, Sheehyun,Shin, Jong-Yeon,Kim, Sujung,Yu, Saet-Byeol,Lee, Won-Chul,Lee, Seungbok,Park, Hansoo,Kim, Jong-Il,Seo, Jeong-Sun Oxford University Press 2011 Nucleic acids research Vol.39 No.1
<P>High-throughput genomic technologies have been used to explore personal human genomes for the past few years. Although the integration of technologies is important for high-accuracy detection of personal genomic variations, no databases have been prepared to systematically archive genomes and to facilitate the comparison of personal genomic data sets prepared using a variety of experimental platforms. We describe here the Total Integrated Archive of Short-Read and Array (TIARA; http://tiara.gmi.ac.kr) database, which contains personal genomic information obtained from next generation sequencing (NGS) techniques and ultra-high-resolution comparative genomic hybridization (CGH) arrays. This database improves the accuracy of detecting personal genomic variations, such as SNPs, short indels and structural variants (SVs). At present, 36 individual genomes have been archived and may be displayed in the database. TIARA supports a user-friendly genome browser, which retrieves read-depths (RDs) and log2 ratios from NGS and CGH arrays, respectively. In addition, this database provides information on all genomic variants and the raw data, including short reads and feature-level CGH data, through anonymous file transfer protocol. More personal genomes will be archived as more individuals are analyzed by NGS or CGH array. TIARA provides a new approach to the accurate interpretation of personal genomes for genome research.</P>
Hong, Young Joon,Jeong, Myung Ho,Choi, Yun Ha,Ko, Jum Suk,Lee, Min Goo,Kang, Won Yu,Lee, Shin Eun,Kim, Soo Hyun,Park, Keun Ho,Sim, Doo Sun,Yoon, Nam Sik,Youn, Hyun Ju,Kim, Kye Hun,Park, Hyung Wook,Kim Oxford University Press 2011 European heart journal Vol.32 No.16
<P><B>Aims</B></P><P>We used virtual histology-intravascular ultrasound (VH-IVUS) to evaluate the relation between coronary plaque characteristics and no-reflow in acute coronary syndrome (ACS) patients.</P><P><B>Methods and results</B></P><P>A total of 190 consecutive ACS patients were imaged using VH-IVUS and analysed retrospectively. Angiographic no-reflow was defined as TIMI flow grade 0, 1, and 2 after stenting. Virtual histology-intravascular ultrasound classified the colour-coded tissue into four major components: fibrotic, fibro-fatty, dense calcium, and necrotic core (NC). Thin-cap fibroatheroma (TCFA) was defined as focal, NC-rich (≥10% of the cross-sectional area) plaques being in contact with the lumen in a plaque burden ≥40%. Of the 190 patients studied at pre-stenting, no-reflow was observed in 24 patients (12.6%) at post-stenting. The absolute and %NC areas at the minimum lumen sites (1.6 ± 1.2 vs. 0.9 ± 0.8 mm<SUP>2</SUP>, <I>P</I> < 0.001, and 24.5 ± 14.3 vs. 16.1 ± 10.6%, <I>P</I> = 0.001, respectively) and the absolute and %NC volumes (30 ± 24 vs. 16 ± 17 mm<SUP>3</SUP>, <I>P</I> = 0.001, and 22 ± 11 vs. 14 ± 8%, <I>P</I> < 0.001, respectively) were significantly greater, and the presence of at least one TCFA and multiple TCFAs within culprit lesions (71 vs. 36%, <I>P</I> = 0.001, and 38 vs. 15%, <I>P</I> = 0.005, respectively) was significantly more common in the no-reflow group compared with the normal-reflow group. In the multivariable analysis, %NC volume was the only independent predictor of no-reflow (odds ratio = 1.126; 95% CI 1.045–1.214, <I>P</I> = 0.002).</P><P><B>Conclusion</B></P><P>In ACS patients, post-stenting no-reflow is associated with plaque components defined by VH-IVUS analysis with larger NC and more TCFAs.</P>
Hemophagocytic lymphohistiocytosis diagnosed by brain biopsy
Ju, Hee Young,Hong, Che Ry,Kim, Sung Jin,Lee, Ji Won,Kim, Hyery,Kang, Hyoung Jin,Park, Kyung Duk,Shin, Hee Young,Chae, Jong-Hee,Phi, Ji Hoon,Cheon, Jung-Eun,Park, Sung-Hye,Ahn, Hyo Seop The Korean Pediatric Society 2015 Clinical and Experimental Pediatrics (CEP) Vol.58 No.9
Hemophagocytic lymphohistiocytosis (HLH) is characterized by fever, splenomegaly, jaundice, and pathologic findings of hemophagocytosis in bone marrow or other tissues such as the lymph nodes and liver. Pleocytosis, or the presence of elevated protein levels in cerebrospinal fluid, could be helpful in diagnosing HLH. However, the pathologic diagnosis of the brain is not included in the diagnostic criteria for this condition. In the present report, we describe the case of a patient diagnosed with HLH, in whom the brain pathology, but not the bone marrow pathology, showed hemophagocytosis. As the diagnosis of HLH is difficult in many cases, a high level of suspicion is required. Moreover, the pathologic diagnosis of organs other than the bone marrow, liver, and lymph nodes may be a useful alternative.
Hong, Yun-Gi,Moon, Yu-Mi,Hong, Ju-Won,No, So-Young,Choi, Tae-Rim,Jung, Hye-Rim,Yang, Soo-Yeon,Bhatia, Shashi Kant,Ahn, Jung-Oh,Park, Kyung-Moon,Yang, Yung-Hun Elsevier 2018 Enzyme and microbial technology Vol.118 No.-
<P><B>Abstract</B></P> <P>Glutaric acid is one of the promising C5 platform compounds in the biochemical industry. It can be produced chemically, through the ring-opening of butyrolactone followed by hydrolysis. Alternatively, glutaric acid can be produced via lysine degradation pathways by microorganisms. In microorganisms, the overexpression of enzymes involved in this pathway from <I>E. coli</I> and <I>C. glutamicum</I> has resulted in high accumulation of 5-aminovaleric acid. However, the conversion from 5-aminovaleric acid to glutaric acid has resulted in a relatively low conversion yield for unknown reasons. In this study, as a solution to improve the production of glutaric acid, we introduced <I>gabTD</I> genes from <I>B. subtilis</I> to <I>E. coli</I> for a whole cell biocatalytic approach. This approach enabled us to determine the effect of co-factors on reaction and to achieve a high conversion yield from 5-aminovaleric acid at the optimized reaction condition. Optimization of whole cell reaction by different plasmids, pH, temperature, substrate concentration, and cofactor concentration achieved full conversion with 100 mM of 5-aminovaleric acid to glutaric acid. Nicotinamide adenine dinucleotide phosphate (NAD(P)<SUP>+</SUP>) and α-ketoglutaric acid were found to be critical factors in the enhancement of conversion in selected conditions. Whole cell reaction with a higher concentration of substrates gave 141 mM of glutaric acid from 300 mM 5-aminovaleric acid, 150 mM α-ketoglutaric acid, and 60 mM NAD<SUP>+</SUP> at 30 °C, with a pH of 8.5 within 24 h (47.1% and 94.2% of conversion based on 5-aminovaleric acid and α-ketoglutaric acid, respectively). The whole cell biocatalyst was recycled 5 times with the addition of substrates; this enabled the accumulation of extra glutaric acid.</P> <P><B>Highlights</B></P> <P> <UL> <LI> The first <I>E. coli</I> whole cell bioconversion from 5-aminovalerate to glutaric acid. </LI> <LI> Finding of critical factors for GabTD reaction. </LI> <LI> Achievement of high bioconversion rate over 90% based on α-ketoglutarate concentration. </LI> <LI> Repetitive use of whole cell biocatalyst to accumulate more glutaric acid. </LI> </UL> </P>
SB203580, a P38 MAPK Inhibitor, Blocks in vitro Invasion by Human Gastric SNU-638 Cells
Ju Chae Park,Hyeon GyeungY oo,Hong Su Kim,Min A Jung,Mi Ha Kim,Sang Won Han,Kee Oh Chay,Boo Ahn Shin,Bong Whan Ahn,Young Do Jung 대한암학회 2002 Cancer Research and Treatment Vol.34 No.6
Purpose: The role of P38 mitogen-activated proteinkinase (MAPK) in gastric cancer invasion has not yetbeen determined. In this study, we examined the effectsof SB203580, a specific P38 MAPK inhibitor, on the invitro invasion of gastric cancer and upon the moleculesinvolved in this process.Materials and Methods: Human gastric cancer SNU-638cells were maintained in RPMI 1640 supplemented with10% FBS. BIOCOAT matrigel invasion chambers wereused to examine in vitro invasiveness, zymography forgelatinase activity, CAT assay for uPA promoter activityand Western and Northern blotting to determine proteinand mRNA levels, respectively.Results: Treatment of SNU-638 cells with SB203580, aspecific P38 MAPK inhibitor, reduced in vitro invasiveness,dose-dependently. SB203580 treatment was foundto decrease both mRNA expression and uPA promoteractivity in gastric SNU-638 cells. In vitro invasion ofSNU-638 cells was partially abrogated by uPA-neutralizingantibodies. The activities of MMPs were not significantlyaltered by SB203580.Conclusion: Our results suggest that P38 MAPK is apotential therapeutic target for inhibiting uPA-dependentgastric tumor invasiveness and metastasis. (Cancer Res Treat. 2002;34:426-431)