Seasonal variation in the incidence of eruptive pseudoangiomatosis: an another evidence for the association with mosquito bites

( Geun-whi Park ),( Tae-wook Kim ),( Sung-min Park ),( Hyun-joo Lee ),( Hyun Ju Jin ),( Hyang-suk You ),( Woo-haing Shim ),( Gun-wook Kim ),( Hoon-soo Kim ),( Hyun-chang Ko ),( Byung-soo Kim ),( Moon- 대한피부과학회 2017 대한피부과학회 학술발표대회집 Vol.69 No.2

Background: Eruptive pseudoangiomatosis (EPA) is acute, self-limiting dermatosis characterized by the sudden onset of bright red angioma-like papules often surrounded by pale halo. The pathogenesis of EPA is still unknown, but insect bites, especially mosquito have been proposed as a potential causative factor of the disease. Objectives: To clarify the relationship between mosquito bites and EPA after analyzing seasonal variation in the incidence of EPA. Methods: We retrospectively analyzed the clinical data of 55 patients with EPA at Pusan National University Hospitals (Busan and Yangsan) from 2005 to 2016. Results: Forty-eight (87.2%) of 55 patients were diagnosed as EPA in autumn (25/55, 45.4%), and summer (23/55, 41.8%). Seventeen patients (30.9%) admitted the history of mosquito bites. EPA incidence in autumn showed the tendency to increase and reached the peak in 2016. Interestingly, according to the press release, mosquito population in the autumn is increasing steadily. Conclusion: Though more studies are needed to elucidate clear relationship between EPA and mosquito bite, mosquito bite could be the very causative factor of EPA based on the result of this study.

Effects of 3-dimensional balance trainer in combination with a video-game system on balance and gait ability in subacute stroke patients

Ha, Hyun Geun,Ko, Young Jun,Lee, Hwang Jae,Lee, Wan Hee korean Academy of Physical Therapy Rehabilitation 2014 Physical therapy rehabilitation science Vol.3 No.1

Objective: The purpose of this study was to investigate the effects of a three dimensional balance trainer in combination with a video-game system and visual feedback on balance recovery and gait function in subacute stroke patients. Design: Randomized controlled trial. Methods: Twenty-three subacute stroke patients were assigned to either an experimental (n=12) or a control group (n=11) using a random permuted block design and sealed envelopes. The experimental group received additional 3-dimensional balance training combined with visual feedback and a game program for 30 min/day, 5 days/week for 4 weeks. Both groups received 30-min of conservative physical therapy sessions based on neurodevelopmental therapy. Before and after the 20 sessions, walking abilities were evaluated by the the GaitRite system and balances were evaluated using the Berg Balance Scale (BBS). The Trunk Impairment Scale (TIS) was used to assess trunk muscle performances. Results: After the 4-week intervention, BBS and TIS scores were significantly increased in both groups (p<0.05), and increases in these scores were significantly greater in the experimental group (p<0.05). After the 4-week intervention, gait speed and cadence were significantly increased in both groups (p<0.05), and as was observed for BBS and TIS scores, changes of gait speed and cadence were significantly greater in the experimental group (p<0.05). Conclusions: The study shows that the 3-dimensional balance trainer combined with visual biofeedback and a video-game system provides a therapeutic means for improving balance and gait ability in subacute stroke patients.

Effects of different foods and temperatures on larval weight of Protaetia brevitarsis (Coleoptera: Scarabaeidae)

Myung-Ha Song,Hong-Geun Kim,Kyu-Won Kwak,Seok-Hyun Lee,Kwan-Ho Park,Won-Tae Kim,Ji-Young Choi 한국응용곤충학회 2016 한국응용곤충학회 학술대회논문집 Vol.2016 No.04

The white-spotted flower chafer, Protaetia brevitarsis (Coleoptera: Scarabaeidae), is traditionally considered to be an important source of medicinal properties of suppression of cancer and prevention of liver-related diseases. Recently, it was listed as a temporal food ingredient by the Korean Ministry of Food and Drug Safety. To use this insect species without endangering wild populations and produce better quality insects, a mass rearing technique and the suitable rearing conditions are needed. The present study was carried out to determine whether different diets and temperatures can affect the larval weight of this beetle. The larval weight change of third instar larvae was tracked for 14 weeks with different combination of three rearing temperatures – 25, 27.5, and 30℃ - and two feed ingredients – soybean cake and rice bran. Among three temperatures, 27.5℃ was the best temperature condition for rearing this beetle. Among four combinations of two feed ingredients, the addition of soybean cake made significant weight increasement for third instar larvae. These results showed that adding soybean cake at 27.5℃ was the best rearing condition for larval weight gain. This rearing condition can be considered in terms of producing better insects and maximizing producers’ benefits.

금화규(Abelmoschus manihot) 꽃 추출물의 In Vitro 피부 미백 및 주름 개선 효능 연구

권현지 ( Hyun-ji Kwon ),범석현 ( Seok-hyun Beom ),현진아 ( Jin-a Hyun ),강은빈 ( Eun-bin Kang ),박하은 ( Ha-eun Park ),한동근 ( Dong-geun Han ),김현정 ( Hyun-jeong Kim ),최은영 ( Eun-young Choi ),안봉전 ( Bong-jeon An ) 대한화장품학회 2021 대한화장품학회지 Vol.47 No.4

본 연구는 화장품 소재로서 금화규(Abelmoschus manihot) 꽃의 미백 활성율 및 항주름 활성을 평가하기 위해 수행하였다. 금화규 꽃은 물과 70% 에탄올을 이용하여 추출하였다. Tyrosinase 억제 활성은 물과 70% 에탄올 추출물 모두 농도 의존적으로 억제 활성이 증가 된다고 평가되었으며, B16F10 세포주에서 생존율에 영향을 미치지 않은 농도인 10, 25, 50, 75, 100 μg/mL에서 멜라닌 생합성을 저해하는 것으로 확인되었다. 금화규 꽃의 항주름 효능으로는 collagenase와 elastase의 활성 저해 능력을 측정한 결과 70% 에탄올 추출물이 물 추출물보다 높은 항주름 활성을 보였다. 본 연구 결과를 통하여 금화규 꽃 추출물은 미백 활성과 항주름 활성을 가지는 기능성 화장품 소재로 사용 가능할 것으로 사료된다. In this study, the skin whitening and anti-wrinkle activity of Abelmoschus manihot (A. manihot) flowers were evaluated as a cosmetic material. A. manihot flowers were extracted using water and 70% ethanol. Tyrosinase inhibitory activity was evaluated to increase concentration-dependent inhibitory activity in both water (AMW) and 70% ethanol extracts (AME), and was found to inhibit melanin biosynthesis at concentrations that did not affect survival in B16F10 cell lines at 10, 25, 50, 75, and 100 μg/mL. As a result of measuring the anti-wrinkle effectiveness of A. manihot flowers, 70% ethanol extract (AME) showed higher anti-wrinkle activity than water extract (AMW). Through the results of this study, it is considered that the A. manihot flower extract can be used as a functional cosmetic material with whitening and anti-wrinkle activity.

찔레꽃(Rosa multiflora) 추출물과 Ascorbic Acid의 시너지 효과를 통한 항산화 활성과 총 페놀함량 및 플라보노이드 함량 분석

범석현 ( Seok-hyun Beom ),권현지 ( Hyun-ji Kwon ),현진아 ( Jin-a Hyun ),강은빈 ( Eun-bin Kang ),박하은 ( Ha-eun Park ),한동근 ( Dong-geun Han ),김현정 ( Hyun-jeong Kim ),최은영 ( Eun-young Choi ),안봉전 ( Bong-jeon An ) 대한화장품학회 2021 대한화장품학회지 Vol.47 No.4

본 연구에서 찔레꽃을 추출, 농축 및 동결 등을 통하여 시료화 하였다. 찔레꽃추출물과 ascorbic acid의 복합물과 단일 ascorbic acid의 항산화 효능을 비교하였다. 본 연구의 목적은 찔레꽃추출물과 ascorbic acid의 시너지 효과를 통한 항산화 효능을 분석하는 것이었다. 실험은 전자공여능, ABTS⁺radical 소거능, SOD 유사 활성, xanthine oxidase 저해 활성, 환원력, FRAP, 폴리페놀 함량 및 플라보노이드 함량을 측정하였다. 그 결과, 전자공여능 실험과 xanthine oxidase 저해 활성 실험에서 복합물이 대조군(ascorbic acid)보다 높은 항산화 활성을 보였다. 플라보노이드 함량 분석 실험의 경우, 100 μg/mL의 농도에서 RMW+A (272.1 mg QE/g), RME+A (90.6 mg QE/g), RMW+A (79.4 mg QE/g)이 대조군(19.0 mg QE/g)보다 함량이 더 높았다. 따라서 찔레꽃추출물과 ascorbic acid 복합물은 단독 사용한 경우에 비하여 시너지 효과를 발휘했다. 따라서 이 복합물은 항산화 화장품에 사용할 경우 제품의 안정화 및 피부노화 억제에 기여할 것으로 사료된다. In this study, Rosa multiflora was sampled through extraction, concentration, and freezing. The antioxidant efficacy of a mixture of R. multiflora extract and ascorbic acid was compared with a single ascorbic acid. The purpose of this study was to analyze the antioxidant efficacy through the synergistic effect of R. multiflora extract and ascorbic acid. The experiment measured electron donating ability, ABTS⁺ radical scavenging, SOD-like activity, xanthine oxidase inhibition activity, reducing power, FRAP, polyphenolic content measurement, and flavnoid content measurement. As a result, the complex showed higher antioxidant activity than the control group (ascorbic acid) in the electron donating ability test and the xanthine oxidase inhibitory activity test. In the case of the flavonoid content experiment, it was confirmed that RMW+A (272.1 mg QE/g), RME+A (90.6 mg QE/g), and RMW+A (79.4 mg QE/g) at a concentration of 100 μg/mL had a higher flavonoid content than the control group (19.0 mg QE/g). Therefore, the extract and ascorbic acid complex exhibited a synergistic effect compared to the single use. Therefore, it is thought that this complex will contribute to product stabilization and skin aging inhibition when used in antioxidant cosmetics.

GM-CSF가 골수줄기세포의 이동에 미치는 영향

한동하 ( Dong Ha Han ),곽민근 ( Min Geun Kwak ),최병현 ( Byung Hyune Choi ),하윤 ( Yoon Ha ),박현선 ( Hyun Seon Park ),민병현 ( Byoung Hyun Min ),우제홍 ( Ze Hong Woo ),박소라 ( So Ra Park ) 한국조직공학과 재생의학회 2005 조직공학과 재생의학 Vol.2 No.3

The granulocyte macrophage colony-stimulating factor (GM-CSF) is known to play an important role in the hematopoiesis of bone marrow-derived cells and the modulation of their functions as well as the maintenance of homeostasis and overall immune competence. In this study, we investigated the effects of GM-CSF on the activation of bone marrow stem cells in rats. GM-CSF was treated to SD rats via intraperitoneal injection everyday at 10 mg/ml for 5 consecutive days. After 6 days, the bone marrow (BM) and peripheral blood (PB) mononuclear cells (MNCs) were collected to analyze the colony forming units-fibroblasts (CFU-F), colony forming units-granulocyte macrophage (CFU-GM), and burst-forming units-erythroid (BFU-E). In result, the numbers of MNCs and the CFU-GM and BFU-E counts in BM were all decreased, while those of PB increased. These results suggest that GM-CSF induced the proliferation and mobilization of MNCs and hematopoietic stem cells (HSCs) to PB. The CFU-F counts showed no significant increase both in BM and PB suggesting that GM-CSF showed no significant effects on the proliferation of mesenchymal stem cells. In contrast, the increase in the alkaline phosphatase expression of CFU-F colonies in BM indicates that GM-CSF might induce the differentiation of MSCs at least to some extent. In conclusion, GM-CSF not only plays important roles in proliferation and differentiation of stem cells in BM but also promotes migration of HSCs into PB.

AhR 발현을 통한 콤부차 분획물의 안티폴루션 효과 연구

강은빈,현진아,권현지,범석현,한동근,박하은,김현정,곽기성,안봉전,Kang, Eun-Bin,Hyun, Jin-A,Kwon, Hyun-Ji,Beom, Seok-Hyun,Han, Dong-Geun,Park, Ha-Eun,Kim, Hyun-Jeong,Kwak, Ki-Sung,An, Bong-Jeon 대한화장품학회 2021 대한화장품학회지 Vol.47 No.3

본 연구에서는 설탕을 넣은 녹차나 홍차에 유익균을 첨가해 발효시킨 음료인 콤부차를 컬럼 크로마토그래피를 이용해 분획한 후, TLC를 통해 플라보노이드류인 퀘르세틴 글리코사이드(quercetin glycoside)의 유효성분 유무를 확인하고 그 분획물을 이용하여 공해로부터 피부를 보호 및 개선하는 안티폴루션 효과를 확인하였다. 인간 각질형성세포에 Kombucha-quercetin glycoside (K-QG)를 처리하여 세포 생존율을 확인한 결과, 100 ㎍/mL 농도까지 90% 이상의 생존율을 보였으며, 안티폴루션 효과를 보기 위해 benzo[e]pyrene과 미세먼지 자극에 의한 세포 생존율을 측정한 결과, 100 ㎍/mL에서 각각 68.79%, 50.68%의 개선율을 보였다. 또한, benzo[a]pyrene에 의해 활성화되는 aryl hydrocarbon receptor (AhR) 발현을 웨스턴 블롯을 통해 확인한 결과, 대조군에 대비하여 100 ㎍/mL에서 31.08%의 억제율을 보였다. 본 연구의 결과를 통해 K-QG는 벤조피렌과 미세먼지로부터 자극받은 피부를 보호, 개선해주며 안티폴루션 기능성 소재로써 가치가 있는 것으로 사료된다. In this study, Kombucha, a fermented drink by adding beneficial bacteria to green tea or black tea with sugar, was classified using column chromatography, and the presence or absence of quercetin glycoside, a type of flavonoids, was confirmed through TLC. In addition, the anti-pollution effect of protecting and improving the skin from pollution was confirmed using the fraction. Keratinocytes were treated with K-QG to confirm the cell viability, showing a survival rate of 90% or more up to a concentration of 100 ㎍/mL, and the cell viability by benzo[e]pyrene and fine dust stimulation to see the anti-pollution effect was measured. At 100 ㎍/mL, the improvement rates were about 68.79% and 50.68%, respectively. In addition, as a result of confirming the expression of AhR activated by benzo[a]pyrene through Western blot, it showed an inhibition rate of about 31.08% at 100 ㎍/mL compared to the control group. Through the results of this study, K-QG is thought to be valuable as an anti-pollution functional material, protecting and improving skin irritated from benzopyrene and fine dust.

Enantioselective phase-transfer catalytic α-alkylation of 2-methylbenzyl <i>tert</i>-butyl malonates

Ha, Min Woo,Hong, Suckchang,Park, Cheonhyoung,Park, Yohan,Lee, Jihye,Kim, Mi-hyun,Lee, Jihoon,Park, Hyeung-geun The Royal Society of Chemistry 2013 Organic & biomolecular chemistry Vol.11 No.24

<P>A new asymmetric synthetic method to prepare alpha,alpha-dialkylmalonates for the construction of a quaternary carbon center via phase-transfer catalytic (PTC) alkylation has been developed. Enantioselective alpha-alkylation of 2-methylbenzyl tert-butyl alpha-methylmalonates under phase-transfer catalytic conditions in the presence of (S,S)-3,4,5-trifluorophenyl-NAS bromide (10) afforded the corresponding alpha,alpha-dialkylmalonates in high chemical (up to 99%) and optical yields (up to 91% ee), which were selectively hydrolyzed to malonic monoacids under alkali basic conditions for conversion to versatile chiral intermediates.</P>

Cloning and Characterization of GDP-mannose Pyrophosphorylase from Solanum Tuberosum L.

Hyun, Tae-Kyung,Lim, Jung-Dae,Kim, Jae-Kwang,Seong, Eun-Soo,Lee, Jae-Geun,Yoon, Byeong-Sung,Kim, Myong-Jo,Cho, Dong-Ha,Yu, Chang-Yeon The Korean Society of Medicinal Crop Science 2005 韓國藥用作物學會誌 Vol.13 No.5

Ascorbic acid is a great antioxidant and helps protect the body against pollutants. GDP-mannose pyrophosphorylase (GMPase) is a key enzyme in manufacturing GDP-mannose, a glycosyl donor for ascorbate and cell wall biosynthesis as well as for protein glycosylation. In this study, we described molecular cloning of a full-length cDNA from Potato (Solanum tuberosum L. cv. Jasim), using tuber. The cDNA isolated encoded a GDP-mannose pyrophosphrylase. The nucleotide sequence of pGMPC showed about 95%, 89% and 80% homology with S. tuberosum (AF022716), N. tabacum (AB066279) and A. thaliana (AF076484) cDNAs clone known as GMPase, respectively. We detected the expression of GMPase using RT-PCR. The highest expression of GMPase was found in stems, and the largest amount of ascorbic acid was also presented in stems. In contrast, the leaf showed minimal level of GMPase transcript and ascorbic acid content. We propose that GMPase expression patterns were similar to the changes of ascorbic acid content in the leaves treated with diverse stresses.

[P2-27] Monitoring on Trace Metals of Shellfishes in Gyeongnam Area

Hyun Kim,Young-Hun Kim,Ho-Hyek Chung,Hui-Geun Cha,Hwang-Chan Bae,Gang-Ja Ha,Young-Rok Kim,Jong-Hwa Chung 한국식품영양과학회 2005 한국식품영양과학회 학술대회발표집 Vol.2005 No.10