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Simultaneous Detection of Three Tobamoviruses in Cucurbits by Rapid Immunofilter Paper Assay
Park, Gug-Seoun,Kim, Jae-Hyun,Chung, Bong-Nam,Kim, Hyun-Ran,Park, Yong-Mun The Korean Society of Plant Pathology 2001 Plant Pathology Journal Vol.17 No.2
A multi-rapid immunofilter paper assay (multi-RIPA) system was prepared for simultaneous diagnosis of three Tobamoviruses, Cucumber green mottle mosaic virus (CGMMV), Kyuri green mottle mosaic virus (KGMMV), and Zucchini green mottle mosaic virus (ZGMMV) in cucurbitaceous crops. Each of these viruses was specifically detected by the multi-RIPA from cucumber, watermelon, zucchini, and bottle gourd inoculated with the three Tobamoviruses singly or in combination. The three viruses could infect cucumber, watermelon, and bottle gourd ; however, CGMMV could not infect zucchini as the latex-coated CGMMV antibody showed a negative reaction in the multi-RIPA of the virus-infected plant extract. When the minimum detection level of multi-RIPA was compared with that of double antibody sandwich-enzyme-linked immunosorbent assay (DAS-ELISA) using CGMMV, the latter was 10 times more sensitive than the former. The detection limit of the multi-RIPA for the purified CGMMV was 50 ng/ml. In a survey of the threeviruses in cucurbits growing in commercial fields in 1999 and 2000, CGMMV was detected in watermelon and cucumber, and ZGMMV was detected only in zucchini growing in plastic houses at the suburbs of Chonju, Korea. However, KGMMV was not found in the commercially growing cucurbit crops in our study, The results suggest that the multi-RIPA can be a simple, rapid, specific and convenient tool to detect simultaneously the Tobamoviruses.
Characterization of Cucumber mosaic virus Isolated from Water Chickweed(Stellaria aquatica)
Park, Gug-Seoun,Kim, Jae-Hyun,Kim, Jeong-Soo,Park, Jang-Kyung The Korean Society of Plant Pathology 2004 Plant Pathology Journal Vol.20 No.2
A strain of Cucumber mosaic virus (CMV) was isolated from a weed, water chickweed (Stellaria aquatica), growing in the pepper field in Chunchon, Korea. This isolate, CMV-Sa, was differentiated from other CMVs based on biological properties and nucleotide sequence analysis of the coat protein (CP) gene. CMV-Sa showed different reactions to all the tested plants, except Capsicum annuum and Cucumis sativus, when compar-ed with those of CMV-Mf (subgroup I) and CMV-PaFM (subgroup II). Remarkably, in Nicotiana tabacum cvs. Samsun, Xanthi-nc and Ky-57, CMV-Sa induced local necrotic ring spots on the inoculated leaves and venal wave pattern and mosaic on the upper leaves. RNA analysis, serology, and RT-PCR of CP gene showed that CMV-Sa belonged to subgroup I of CMV. However, restriction enzyme analysis of the cDNA using AluI, HhaI, HincII, HindIII, HinfI and MspI showed that CMV-Sa was distinct from that of CMV-Mf. Based on comparison of the nucleotide of CP gene and deduced amino acid sequences between other CMV strains, CMV-Sa was closely related to CMV-Mf with 93.7% and 97.2 % identity, respectively.
Genetic Distribution of Carbapenem-Resistant Enterobacteriaceae in Seoul Korea, 2018~2020
Sang-Hun Park,So-Hyun Park,Jin-Seok Kim,Jin-Kyung Yu,Jin-Kyoung Kim,Hyo-Sun Suh,Eun Young Kwon,Kyoung Ae Park,Eui Kyung Cha,Jae Min Shin,Hyo-Won Jeoung,Su-Jin Jeon,Young-Ok Hwang,Jib-Ho Lee,Yong-Seoun 대한바이러스학회 2022 Journal of Bacteriology and Virology Vol.52 No.1
Characterization of Melon necrotic spot virus Isolated from Muskmelon
Park, Gug-Seoun,Kim, Jae-Hyun,Kim, Jeong-Soo The Korean Society of Plant Pathology 2003 Plant Pathology Journal Vol.19 No.2
A severe disease of muskmelon (Cucumis melo cv. Alsnight) grown on rockwool in a plastic house was characterized by leaf and stem necrosis followed by death of the plants. In 2001, an isolate of Melon necrotic spot virus-MN (MNSV-MN) of the genus Camovirus was identified as the causal agent of the disease on the basis of biological reactions and nucleotide sequence analyses of coat protein (CP) gene. MNSV-MN induced necrotic local lesions on mechanically inoculated leaves and systemic necrotic spots on the upper leaves of melon cvs. Alsnight, Rui III, Party, Imperial, and Seolhang. However, the inoculated leaves of watermelon and cucumber showed only necrotic lesions. DsRNAs extracted from the melon infected with MNSV-MN were separated into three components. Molecular sizes of the dsRNAs were estimated at approximately 4.5, 1.8, and 1.6 kbp. The amplified cDNA products of CP gene for MNSV-MN by RT-PCR showed approximately 1.2 kbp. The amplified DNA was digested to three fragments by MspI treatment. The cDNA of the genomic RNA of MNSV-MN was cloned and the region deduced to encode the CP was sequenced. The CP coding region, located near 3' end of the genome, consisted of 1,170 nucleotides and had the potential to encode a 390 amino acid protein. The nucleotide and amino acid sequences of MNSV-MN CP gene were 84.0-94.6% and 90.8-94.9% identical with other MNSV isolates found in the GeneBank database, respectively. This is the first report on the occurrence of MNSV in Korea.
Kim, Hyun-Ran,Park, Yong-Mun,Chung, Bong-Nam,Park, Gug-Seoun,Kim, Jeong-Soo The Korean Society of Plant Pathology 2002 Plant Pathology Journal Vol.18 No.5
Grapevine leafroll-associated 3 virus (GLRaV-3) and Grapevine fanleaf virus (GFLV) are important viral diseases of grapevine in the world. In this study, the most reliable woody indicator plants were selected for virus indexing. Two grapevines, LN33 (Couderc 1613x vitis berlandieri) and Vitis riparia Gloire, were selected for CLRaV-3 and CFLV graft indexing, respectively. The specific characteristics of Closterovirus isolated from grapevines cultivated in Korea were identified. filamentous virus-like particles only existed in the phloem parenchyma cell. In particular, the vesiculation of mitochondria was observed. This mitochondrial vesicu-lation was considered to be one of the most reliable cytopathic features of Closterovirus. During observation of GFLV-infected Chenopodium quinoa sections, virus-like particles arranged consistently were found forming several layers in cytoplasm. Moreover, virus-like particles in tubules were observed and were associated with plasmodesmata in cytoplasm. This is the first report on cytopathological characteristics of Closterovirus and Nepovirus identified from grapevines in Korea.
대형과립 림프구성 백혈병(자연살세포증식증) 1예 : NK Cell Proliferative Disorder
김현아,이기형,박영석,서창인,허대석,박선양,김병국,김노경,이남용,조한익,김철우 대한내과학회 1992 대한내과학회지 Vol.42 No.2
저자들은 전신동통을 주소로 입원한 환자에서 말초혈액 및 골수에서 LGL의 이상 증식을 발견하고, 이들 세포의 표식자가 CD3(-), CD4(-), CD8(-), CD16(+), CD56(+)로 NK cell에 특이하며, 환자의 말초혈액 소견상 NK ceoo 활성의 이상 증진을 보여 대형과립 림프구성 백혈병(자연살세포중식중)으로 진단된 1예를 국내 최초로 경험하였기에 문헌 고찰과 함께 보고 하는 바이다. Natural killer cells comprise 5 to 15% of human peripheral blood lymphocytes (PBL) and most have the morpholgy of large granular lymphocytes (LGL). They have been implicated in several activities in vivo, including destruction of tumor cells, resistance to viral infections, and regulation of hematopoiesis. LGL/NK cell proliferative disorder is a recently recognized, relatively rare atypical lymphocytosis characterized by the presence of over 2000/㎣ lymphocytes with cytoplasmic azurophilic granule sin the peripheral blood. The clinical course is heterogenous, varying from spontaneous regression to progressive malignant disease. We experienced a case of lymphoproliferative disease of large granular lymphocytes, which expressed the NK cell surface marker, CD16 and CD56. The patient was a 56-year old man who presented with generalized myalgia. Physical examination revealed non-tender and firm hepatomegaly. The peripheral blood smear showed increment of lymphocytes up to 12,166/㎣, 50~60% of which were large granular lymphocytes. On bone marrow examination, LGL's were estimated to be about 30% and the granulocytic, erythrocytic and megakaryocytic series were normal in number and morphology, Flow cytometric analysis demonstrated that 71.9% and 77.9%~82.5% of peripheral blood lymphocytes expressed CD16 and CD56, respectively. Also, the cells exhibited markedly increased cytolytic activity against both K562 and Daudi cell lines. There were no signs of lymphadenopathy or splenomegaly, and attempt of evaluating the hepatomegaly by peritoneoscopic biopsy failed due to peritoneal adhesion from previous laparotomy. Generalized bodyache was somewhat alleviated by acetaminophen. He was discharged and followed up for 6months without any serious problems. This paper described the first case of LGL/NK cell proliferative disorder in Korea.
Chrysanthemum stunt viroid in Dendranthema grandiflorum
Chung, Bong-Nam,Park, Gug-Seoun,Kim, Hyun-Ran,Kim, Jeong-Soo The Korean Society of Plant Pathology 2001 Plant Pathology Journal Vol.17 No.4
Chrysanthemum stunt viroid (CSVd) ws identified in chrysanthemum cv. Chunkwang showing symptoms of stunt with leaf distortion (K1) and stunt with chlorosis of leaves (K2) collected from the main cultivation area of Masan, Kyongnam province in Korea. The specific RNAs related with the diseased chrysanthemums were detected. Full-length 354 bp CSVd cDNAs were amplified from infected tissue by reverse transcription and polymerase chain reaction using a pair of primers specific for CSVd sequence. The amplified cDNA products were analyzed by agarose gel electrophoresis and the specific cDNAs were cloned. Nucleotide sequences of the two CSVd isolates K1 and K2 varied. Phylogenetic analysis of the nucleotide sequences of CSVd isolates indicated that K1 was closely related with J2 and Am 2 isolates. K1 and K2 were transmitted by grafting to Dendranthema grandiflorum cv. Mistletoe, Gynura aurantiaca, and Lycopersicon esculentum cv. Rutgers. This is the first report of CSVd in D. grandiflorum in Korea.