Exogenous p53 Upregulated Modulator of Apoptosis (PUMA) Decreases Growth of Lung Cancer A549 Cells

Liu, Chun-Ju,Zhang, Xia-Li,Luo, Da-Ya,Zhu, Wei-Feng,Wan, Hui-Fang,Yang, Jun-Ping,Yang, Xiao-Jun,Wan, Fu-Sheng Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.2

Purpose: To investigate the influence of exogenous p53 upregulated modulator of apoptosis (PUMA) expression on cell proliferation and apoptosis in human non-small cell lung cancer A549 cells and transplanted tumor cell growth in nude mice. Materials and Methods: A549 cells were divided into the following groups: control, non-carrier (NC), PUMA (transfected with pCEP4-(HA) 2-PUMA plasmid), DDP ($10{\mu}g/mL$ cisplatin treatment) and PUMA+DDP (transfected with pCEP4-(HA)2-PUMA plasmid and $10{\mu}g/mL$ cisplatin treatment). The MTT method was used to detect the cell survival rate. Cell apoptosis rates were measured by flow cytometry, and PUMA, Bax and Bcl-2 protein expression levels were measured by Western blotting. Results: Compared to the control group, the PUMA, DDP and PUMA+DDP groups all had significantly decreased A549 cell proliferation (p<0.01), with the largest reduction in the PUMA+DDP group. Conversely, the apoptosis rates of the three groups were significantly increased (P<0.01), and the PUMA and DDP treatments were synergistic. Moreover, Bax protein levels significantly increased (p<0.01), while Bcl-2 protein levels significantly decreased (p<0.01). Finally, both the volume and the weights of transplanted tumors were significantly reduced (p<0.01), and the inhibition ratio of the PUMA+DDP group was significantly higher than in the single DDP or PUMA groups. Conclusions: Exogenous PUMA effectively inhibited lung cancer A549 cell proliferation and transplanted tumor growth by increasing Bax protein levels and reducing Bcl-2 protein levels.

Analysis of Geographic and Pairwise Distances among Chinese Cashmere Goat Populations

Liu, Jian-Bin,Wang, Fan,Lang, Xia,Zha, Xi,Sun, Xiao-Ping,Yue, Yao-Jing,Feng, Rui-Lin,Yang, Bo-Hui,Guo, Jian Asian Australasian Association of Animal Productio 2013 Animal Bioscience Vol.26 No.3

This study investigated the geographic and pairwise distances of nine Chinese local Cashmere goat populations through the analysis of 20 microsatellite DNA markers. Fluorescence PCR was used to identify the markers, which were selected based on their significance as identified by the Food and Agriculture Organization of the United Nations (FAO) and the International Society for Animal Genetics (ISAG). In total, 206 alleles were detected; the average allele number was 10.30; the polymorphism information content of loci ranged from 0.5213 to 0.7582; the number of effective alleles ranged from 4.0484 to 4.6178; the observed heterozygosity was from 0.5023 to 0.5602 for the practical sample; the expected heterozygosity ranged from 0.5783 to 0.6464; and Allelic richness ranged from 4.7551 to 8.0693. These results indicated that Chinese Cashmere goat populations exhibited rich genetic diversity. Further, the Wright's F-statistics of subpopulation within total (FST) was 0.1184; the genetic differentiation coefficient (GST) was 0.0940; and the average gene flow (Nm) was 2.0415. All pairwise FST values among the populations were highly significant (p<0.01 or p<0.001), suggesting that the populations studied should all be considered to be separate breeds. Finally, the clustering analysis divided the Chinese Cashmere goat populations into at least four clusters, with the Hexi and Yashan goat populations alone in one cluster. These results have provided useful, practical, and important information for the future of Chinese Cashmere goat breeding.

Preparation of Microspheres Encapsulating a Recombinant TIMP-1 Adenovirus and their Inhibition of Proliferation of Hepatocellular Carcinoma Cells

Xia, Dong,Yao, Hui,Liu, Qing,Xu, Liang Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.12

Objective: The study aim was to prepare poly-DL-lactide-poly (PELA) microspheres encapsulating recombinant tissue inhibitors of metalloproteinase-1 (TIMP-1) in an adenovirus to investigate its inhibition on the proliferation of hepatocellular carcinoma cells HepG2. Methods: Microspheres were prepared by encapsulating the recombinant TIMP-1 adenovirus into biodegradable PELA. The particle size, viral load, encapsulation efficiency and in-vitro release were measured. Microspheres were used to infect HepG2 cells, then infection efficiency was examined under a fluorescent microscope and ultrastructural changes assessed by TEM. Expression of TIMP-1 mRNA in HepG2 cells was examined by semi-quantitative RT-PCR and proliferation by MTT and cell growth curve assays. Results: We successfully prepared microspheres encapsulating recombinant TIMP-1 adenovirus with a diameter of $1.965{\mu}m$, an encapsulation efficiency of 60.0%, a viral load of $10.5{\times}10^8/mg$ and approximate 60% of virus release within 120 h, the total releasing time of which was longer than 240 h. The microspheres were confirmed to be non-toxic with blank microspheres. Infected HepG2 cells could stably maintain in-vitro expression of TIMP-1, with significantly effects on biological behaviour Conclusion: PELA microspheres encapsulating a recombinant TIMP-1 adenovirus can markedly inhibit the proliferation of HepG2 cells, which provides an experimental basis for polymer/chemistry-based gene therapy of hepatocellular carcinomas.

Facile Synthesis of Microsphere Copper Cobalt Carbonate Hydroxides Electrode for Asymmetric Supercapacitor

Liu, S.,Hui, K.S.,Hui, K.N.,Jadhav, V.V.,Xia, Q.X.,Yun, J.M.,Cho, Y.R.,Mane, R.S.,Kim, K.H. Pergamon Press 2016 ELECTROCHIMICA ACTA Vol.188 No.-

Porous microspheres copper cobalt carbonate hydroxides (Cu<SUB>x</SUB>Co<SUB>2@?x</SUB>CH) pseudocapacitive electrode material comprised of nanoplates via a facile hydrothermal method were presented. Significantly, the crystalline structure, morphology and electrochemical performance of the Cu<SUB>x</SUB>Co<SUB>2@?x</SUB>CH can be readily manipulated by varying the Cu/Co molar ratios. Among various stoichiometries of Cu<SUB>x</SUB>Co<SUB>2@?x</SUB>CH porous microspheres studied, Cu<SUB>1.79</SUB>Co<SUB>0.21</SUB>CH consisted of nanoplates with a mean thickness of @?35nm showed a high specific capacitance of 789Fg<SUP>@?1</SUP> at a current destiny of 1Ag<SUP>@?1</SUP> and good rate ability. Furthermore, the optimized Cu<SUB>1.79</SUB>Co<SUB>0.21</SUB>CH electrode also exhibited remarkable high cycling stability, ca 77.5% after 3000 charge/discharge cycles at current density of 5Ag<SUP>@?1</SUP>. An asymmetric device was constructed from optimized Cu<SUB>1.79</SUB>Co<SUB>0.21</SUB>CH materials on nickel foam (NF) as cathode electrode and graphene on NF as anode electrode in a 6M KOH electrolyte. The asymmetric Cu<SUB>1.79</SUB>Co<SUB>0.21</SUB>CH/NF//graphene/NF supercapacitor device delivered a specific capacitance of 60.5Fg<SUP>@?1</SUP> in a potential range of 0@?1.6V. A high energy density of 21.5Whkg<SUP>@?1</SUP> was achieved at the power density of 200Wkg<SUP>@?1</SUP>. More significantly, the designed device exhibited excellent cycling stability with 73.3% capacity retention after 5000 cycles.

First Record of Foot-Rot Caused by Fusarium solani on Pachira aquatica in China

Qiao Xia Shang,Hui Jia,Jin Zhong Wang,Su Hua Liu,Zheng Ping Liu 한국원예학회 2006 한국원예학회 기타간행물 Vol.- No.-

Glass fiber reinforced graphite/carbon black@PES composite films for high-temperature electric heaters

Yaning Liu,Zhen Xiao,Wenkui Zhang,Hui Huang,Jun Zhang,Yongping Gan,Xinping He,Bingjia Wang,Yi Han,Yang Xia 한국공업화학회 2022 Journal of Industrial and Engineering Chemistry Vol.107 No.-

Carbon based conductive polymer composites as electrothermal materials offers many merits in highflexibility, light weight, low cost, excellent processability, and rapid heating ability, however, the maximumworking temperature is usually lower than 200 C, hampering seriously the medium–high temperatureapplications. Herein, a series of rationally designed glass fiber reinforced graphite/carbonblack@polyethersulfone (G/CB@PES) composites are developed to significantly promote the maximumworking temperature over 300 C. To be specific, the introduced glass fibers acted as rigid skeleton greatlyenhance the mechanical strength of PES polymer matrix, thereby offering robust structural stability inhigh temperature working scenario. Meanwhile, glass fibers not only endow G/CB@PES composite withexcellent flame-retardant ability, but also provide rapid thermal conduction channels to reduce the riskof heat accumulation and overheating. More importantly, glass fiber reinforced G/CB@PES composites aselectrothermal film heaters exhibit superior electric heating performance in terms of fast temperatureresponsiveness, high electric heating efficiency, stable maximum working temperatures, and high electricpower efficiency under the relatively low applied voltages of 3–21 V. This work provides new insights onthe innovative design and facile fabrication of high working temperature electrothermal film heaters inemerging applications.

DIETARY SUPPLEMENTAL EFFECTS OF Gracilaria lemaneiformis ON GROWTH PERFORMANCE, HAEMATOLOGICAL PARAMETERS AND SALINITY STRESS OF JUVENILE PACIFIC WHITE SHRIMP (Litopenaeus vannamei)

Li-xia Tian,Ying-ying Yu,Yong-jian Liu,Jin Niu,Wen-dong Chen,Hui-jun Yang,Gui-ying Liang 한국수산과학회 양식분과 2015 한국수산과학회 양식분과 학술대회 Vol.2015 No.5

Clinical Impact of Drug Adherence of Tyrosine Kinase Inhibitors in Children with Ph-Positive Acute Lymphoblastic Leukemia

Jun-Xia Wang,Miao-Miao Yang,Li-Peng Liu,Hui-Min Zhang,Meng-Chuan Wang,Yu-Wen Chen,Xiao-Ying Zang,Fang Hu 대한암학회 2023 Cancer Research and Treatment Vol.55 No.3

Purpose This study aimed to explore the impact of ABL1–tyrosine kinase inhibitors (TKIs) adherence on the survival of chromosome-positive (Ph+) acute lymphoblastic leukemia (ALL) children and clarify the potential predictors of patients’ prognosis from TKIs intake practices.Materials and Methods Ninety newly diagnosed Ph+ ALL patients who received TKIs were enrolled. We collected the baseline characteristics and adverse events in all children; moreover, TKIs adherence was measured by an eight-item Morisky medication adherence scale (MMAS-8). Progression-free survival (PFS) and overall survival (OS) analysis were performed, and risk factors for PFS and OS were evaluated.Results Among all patients, 69 cases were regarded as adherers, while 21 were non-adherers. The median duration of TKIs interruption was significantly prolonged in the non-adherence group than in the adherence group (13 [0-101] vs. 56 [11-128], p < 0.001). Additionally, dose reduction occurred in 55.2% of non-adherers versus 23.0% of adherers (p=0.002). The PFS and OS in adherers were significantly higher versus non-adherers (p=0.020 and p=0.039). MMAS-8 score was an independent risk factor for PFS (p=0.010) and OS (p=0.031). Among non-adherers, the median OS was only 23.1% (4.2%-42%) in patients aged ≤ 10 years versus 54.4% (38.8%-70%) in adolescents. Most of the patients who experienced TKIs non-adherence suffered pancytopenia.Conclusion TKIs adherence during treatment significantly influenced the survival of pediatric Ph+ ALL patients, and non-adherers with age ≤ 10 years were more vulnerable to TKIs disruption. The cumulative TKIs dose should be especially emphasized to patients with age ≤ 10 years, which may result in an inferior achievement of relevant treatment milestones.

Double-Lead Content Search Scheme for Producer Mobility in Named Data Networking

Huilin Liu,Xushan Chen,Guoxin Xia,Hui Guo 한국통신학회 2020 한국통신학회 APNOMS Vol.2020 No.09

In Named Data Networking (NDN), the processing of the packet is based on the data rather than the communication endpoint. It’s very suitable for the core demand of content sharing on the internet today. However, there are still some problems to be dealt with, including mobility support of the producer. By analyzing existing solutions, we put forward a solution finding MCS (Mobile Content Source) passively by HR(Home Repository) and leading interest packets to find MCS actively in the local scope at the same time to speed up to search MCS and to support NDN producer mobility. Triangle routing problem in single HR and low efficiency problem can be based on this solution. Experimental results show that our scheme does better in reducing delay with low handoff cost than other typical solutions.

EDGE COVERING COLORING OF NEARLY BIPARTITE GRAPHS

Wang, Ji-Hui,Zhang, Xia,Liu, Guizhen 한국전산응용수학회 2006 Journal of applied mathematics & informatics Vol.22 No.1

Let G be a simple graph with vertex set V(G) and edge set E(G). A subset S of E(G) is called an edge cover of G if the subgraph induced by S is a spanning subgraph of G. The maximum number of edge covers which form a partition of E(G) is called edge covering chromatic number of G, denoted by X'c(G). It is known that for any graph G with minimum degree ${\delta},\;{\delta}-1{\le}X'c(G){\le}{\delta}$. If $X'c(G) ={\delta}$, then G is called a graph of CI class, otherwise G is called a graph of CII class. It is easy to prove that the problem of deciding whether a given graph is of CI class or CII class is NP-complete. In this paper, we consider the classification of nearly bipartite graph and give some sufficient conditions for a nearly bipartite graph to be of CI class.