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Study of glucose oxidase-l-α-lecithin Langmuir film formation on air-water interface
Fan Yin,Kil-Yong Ryu,Hoon-Kyu Shin,Young-Soo Kwon 한국물리학회 2007 Current Applied Physics Vol.7 No.5
The present paper describes the formation of glucose oxidase (GOx)L-a-lecithin Langmuir lm on airwater interface by spreadingGOx solution directly onto subphase covered with layer of lecithin. The optimum experiment conditions were obtained according to them, which represented the movement of GOx molecules under the lecithin layer and the reorientation of GOx molecules in the lecithinlayer and/or expulsion of GOx molecules from the lecithin layer, respectively. The forces of GOx molecules that interacted with the lec-ithin layer were hydrogen bonding and hydrophobic force. An atomic force microscopy image of GOxlecithin lm deposited on Au(11) surface in optimal conditions gives evidence of well-ordered GOx molecules in the lecithin layer. As a target of this research, this work provides a new way to prepare biomimetic film and design glucose biosensors in future.
Yin Ronghuan,Yin Ronglan,Bai Man,Fan Yixing,Wang Zeying,Zhu Yubo,Zhang Qi,Hui Taiyu,Shen Jincheng,Feng Siyu,Bai Wenlin 아세아·태평양축산학회 2023 Animal Bioscience Vol.36 No.4
Objective: The objective of this study was to investigate the effects of N6-Methyladenosine modification-circRNA-zinc finger protein 638 (m6A-circRNA-ZNF638) on the induced activation of secondary hair follicle (SHF) stem cells with its potential mechanisms in cashmere goats. Methods: The m6A modification of ZNF638 was analyzed using methylation immunoprecipitation with real-time quantitative polymerase chain reaction technique in SHF stem cells. The effects of circRNA-ZNF638 on the induced activation of SHF stem cells in m6A dependence were evaluated through the overexpression of circRNA-ZNF638/its m6Adeficient mutants in circRNA-ZNF638 knockdown SHF stem cells. The competitive binding of miR-361-5p to circRNA-ZNF638/Wnt5a 3′- untranslated region was analyzed through Dual-luciferase reporter assay. Results: The m6A-circRNA-ZNF638 had significantly higher transcription at anagen SHF bulge of cashmere goats compared with that at telogen, as well as it positively regulated the induced activation of SHF-stem cells in cashmere goats. Mechanismly, m6A-circRNA-ZNF638 sponged miR-361-5p to heighten the transcriptional expression of Wnt5a gene in SHFstem cells. We further demonstrated that the internal m6A modification within circRNAZNF638 is required for mediating the miR-361-5p/Wnt5a pathway to regulate the induced activation of SHF stem cells through an introducing of m6A-deficient mutant of circRNAZNF638. Conclusion: The circRNA-ZNF638 contributes the proper induced activation of SHF-stem cells in cashmere goats in m6A-dependent manner through miR-361-5p/Wnt5a axis.
Fan Yin,Sang-Hyun Park,Hoon-Kyu Shin,Young-Soo Kwon 한국물리학회 2006 Current Applied Physics Vol.6 No.4
Hemoglobin (Hb)-octadecylamine (ODA) LangmuirBlodgett lm was formed by spreading Hb solution directly onto subphasecovered with a layer of ODA. The optimum equilibrium time of HSA interacted with ODA layer, the optimum amount of proteinspread on subphase.s interface and the optimum ionic strength and pH of subphase were obtained according to the experimentalresults. The compressibility of HbODA lm in compressing process indicated that at least two types of phase transition occurred.The complex LB lm is very stable and can be used to modify electrode surface. AFM images of complex monolayer deposited onAu (11) surface under dierent surface pressures indicated that rearrangement and reorientation of Hb molecules in ODA lm onAu (11) surface occurred during the phase transition process by compressing monolayer. The well ordered HbODA LB lm onsolid surface can be obtained by depositing it under surface pressure range of 3540 mN/m.
Meiqiang Fan,Yong Jin Zou,Hao Yin,Yan Li Huang,Chengqiao Xi,Haixin Kang,Chao Li,Jingjing Zhang,Chunju Lv,Meiqiang Fan,Zhi Chen 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2019 NANO Vol.14 No.3
A hollow silicon oxide coated with cuprous oxide and polyaniline (hSiOx/Cu2O/PANI) was prepared via Stober method, magnesium reduction and chemical oxidative polymerization. The hSiOx/Cu2O/PANI (SiOx 62 wt.%, PANI 33 wt.%, Cu2O 5 wt.%) presented charge/discharge capacities of up to 2000 mAhg -1 after 60 cycles at 0.2 Ag -1 current density and higher than 880 mAhg -1 at 8 Ag -1 current density. Microstructural analysis demonstrated that the improvement was due to the nanostructure of hollow SiOx sphere coated with Cu2O and PANI, which could release high stress caused by volume expansion during the lithiation/delithiation process and had little damage to electrode materials. Cycle voltammetry (CV) and electrochemical impedance spectroscopy (EIS) results further confirmed that Cu2O and PANI dual-coating improved reversibility and conductivity of hSiOx and prevented it to drop from the electrode surface.
Fan, Juexin,Zhang, Bin,Li, Lili,Xiao, Chaowu,Oladele, Oso Abimbola,Jiang, Guoli,Ding, Hao,Wang, Shengping,Xing, Yueteng,Xiao, Dingfu,Yin, Yulong Asian Australasian Association of Animal Productio 2015 Animal Bioscience Vol.28 No.11
This study was to investigate the effect of soyabean isoflavones (SIF) on onset of puberty, serum hormone concentration, and gene expression in hypothalamus, pituitary and ovary of female Bama miniature pigs. Fifty five, 35-days old pigs were randomly assigned into 5 treatment groups consisting of 11 pigs per treatment. Results showed that dietary supplementation of varying dosage (0, 250, 500, and 1,250 mg/kg) of SIF induced puberty delay of the pigs with the age of puberty of pigs fed basal diet supplemented with 1,250 mg/kg SIF was significantly higher (p<0.05) compared to control. Supplementation of SIF or estradiol valerate (EV) reduced (p<0.05) serum gonadotrophin releasing hormone and luteinizing hormone concentration, but increased follicle-stimulating hormone concentration in pigs at 4 months of age. The expression of KiSS-1 metastasis-suppressor (KISS1), steroidogenic acute regulatory protein (StAR) and 3-beta-hydroxysteroid dehydrogenase/delta-5-delta-4 isomerase ($3{\beta}-HSD$) was reduced (p<0.01) in SIF-supplemented groups. Expression of gonadotropin-releasing hormone receptor in the pituitary of miniature pigs was reduced (p<0.05) compared to the control when exposed to 250, 1,250 mg/kg SIF and EV. Pigs on 250 mg/kg SIF and EV also showed reduced (p<0.05) expression of cytochrome P450 19A1 compared to the control. Our results indicated that dietary supplementation of SIF induced puberty delay, which may be due to down-regulation of key genes that play vital roles in the synthesis of steroid hormones.
Fan Wang,Renliang Yu,Shengnan Wen,Jie Yin,Yugen Shi,Hesheng Hu,Suhua Yan 대한약학회 2021 Archives of Pharmacal Research Vol.44 No.5
Myocardial infarction (MI) is one of the leadingcauses of high mortality worldwide. Long non-codingRNA myocardial infarction associated transcript (MIAT)and mitochondrial coupling factor 6 (CF6) aggravate MI. This study aimed to elucidate whether miR-203 interactedwith MIAT and CF6 in MI. Results revealed that MIAT andCF6 expressions were upregulated and that miR-203 wasdownregulated in mouse myocardial tissues after MI, as wellas in hypoxic mouse cardiomyocytes. The overexpressionof MIAT in mouse cardiomyocytes raised CF6 expression,whereas the knockdown of MIAT had the opposite eff ect. Mechanistically, the luciferase reporter and RNA pull-downassays corroborated the binding between miR-203 and CF63′UTR and between miR-203 and MIAT. The simultaneousoverexpression of miR-203 and MIAT restored the reductionof CF6 caused by miR-203 overexpression alone, andthe overexpression of miR-203 diminished the percentage ofinfarct area and the apoptosis of cardiomyocytes in vivo. Our fi ndings corroborate that overexpressing miR-203 alleviatesMI via interacting with MIAT and CF6.
Fan Yunli,To Michael Kai-Tsun,Yeung Eric Hiu Kwong,Kuang Guan-Ming,Liang Ruixin,Cheung Jason Pui Yin 대한척추외과학회 2023 Asian Spine Journal Vol.17 No.5
Study Design: This study adopted a prospective cohort study design.Purpose: This study aimed to examine electromyogram (EMG) discrepancy in paravertebral muscle activity and scoliosis progression, determine how vertebral morphology and EMG discrepancy evolve during scoliosis progression, and identify differences in EMG activity between individuals with and without adolescent idiopathic scoliosis (AIS).Overview of Literature: Higher EMG activity is observed in the convex side of scoliotic curves, but not in populations without scoliosis, suggesting that higher EMG activity is a causative factor for curve progression.Methods: In this study, 267 matched pairs of AIS and controls were recruited. The participants underwent EMG measurements at their first presentation and did not receive any treatment for 6 months at which point they underwent EMG and radiographs. Early curve progression was defined as >5° in Cobb angle at 6 months. The root mean square of the EMG (rms-EMG) signal was recorded with the participants in sitting and back extension. The rms-EMG ratio at the upper end vertebrae, apical vertebrae (AV), and lower end vertebrae (LEV) of the major curve was calculated.Results: The rms-EMG ratio in the scoliosis cohort was high compared with that in the controls (sitting: 1.2±0.3 vs. 1.0±0.1, <i>p</i><0.01; back extension: 1.1±0.2 vs. 1.0±0.1, <i>p</i><0.01). An AV rms-EMG ratio in back extension, with a cutoff threshold of ≥1.5 in the major thoracic curve and ≥1.3 in the major lumbar curve, was a risk factor for early curve progression after 6 months without treatment (odds ratio, 4.1; 95% confidence interval, 2.8–5.9; <i>p</i><0.01). Increases in side deviation (SD) (distance between the AV and the central sacral line) were related to a higher rms-EMG ratio in LEV of the major thoracic curve (baseline: <i>r<sub>s</sub></i>=0.2, <i>p</i>=0.03; 6 months: <i>r<sub>s</sub></i>=0.3, <i>p</i><0.01).Conclusions: An EMG discrepancy was detected in the scoliosis cohort, which was related to increases in SD in the major thoracic curve. The AV rms-EMG ratio in back extension was correlated with curve progression after 6 months of no treatment.