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김은경 ( Eun-gyeong Kim ),김민경 ( Min-kyung Kim ),권현애 ( Hyun-ae Kwon ),윤도경 ( Do-kyung Youn ),구정헌 ( Jeong-heon Koo ),박소연 ( So-yeon Park ),이희근 ( Hui-geun Lee ),조명희 ( Myeong-hui Jo ),하도윤 ( Do-yun Hah ),김철호 ( 한국동물위생학회(구 한국가축위생학회) 2018 韓國家畜衛生學會誌 Vol.41 No.3
Salmonella Enteritidis (S. Enteritidis) are found in animals, humans, and environment. In addition, S. Enteritidis draws attention to the public health concerns due to carriage of antibiotic resistance traits. For these reasons, the prevalence and antibiotic resistance patterns of S. Enteritidis are significant issues with regard to public health. To address this issues, a total of 24 strains of S. Enteritidis from 164 samples collected from several slaughterhouses in Gyeong-Nam province in order for antibiotic resistance profiles. Subsequently, we characterized the genotyping by random amplification polymorphic DNA (RAPD)-PCR. As a result, very high level of resistance to protein synthesis inhibition antibiotics and most isolates were susceptible to others. Six random primers were used for RAPD-PCR to reveal genotypes of S. Enteritidis isolates. One of the primer, P1245, generated 147 distinct RAPD-PCR fragments ranging from 400∼3000 bp. The number of RAPD-PCR products ranged from 4 to 8 for this primer. The RAPD-PCR fragments could be placed these strains into 3 subgroups and 2 classes by UPGMA cluster analysis. Interestingly, several S. Enteritidis that isolated from different slaughterhouses showed same genotype. These results showed only limited genetic variation among the isolates, those were grouped into a few different patterns of antibiotic resistance.
Case Report : Autosomal Translocation Patient Who Experienced Premature Menopause: A Case Report
( Tae-hee Kim ),( Yesol Kim ),( Do-won Jeong ),( Eun-gyeong Lee ),( Dong-su Jeon ),( Jun-mo Kim3 ) 대한폐경학회 2015 대한폐경학회지 Vol.21 No.2
Premature ovarian failure (POF) is a condition in which the ovarian functions of hormone production and oocyte development become impaired before the typical age for menopause. POF and early menopause are present in a broad spectrum of gonad dysgenesis, from a complete cessation of ovarian function to an intermittent follicle maturation failure. Actually POF has been identified as a genetic entity (especially chromosome X), but data on genetic factors of premature menopause are limited. Until now, several cases revealed that inactivation of X chromosomes has an effect on ages of premature menopause and females with balanced or unbalanced X-autosome translocations can have several reproductive problems. On the other hand, there have been a few data that was caused by autosome-autosome translocation can lead. Therefore we report a relevant case of POF with translocation between chromosomes 1 and 4. She had her first menstrual period at the age of 12, and after 7 years she stopped menstruation. Chromosomal analysis showed 46, XX, t (1;4) (p22.3;q31.3). While evaluating this rare case, we could review various causes (especially genetic factors) of POF. To remind clinicians about this disease, we report a case of POF caused by autosomeautosome translocation with a literature review. (J Menopausal Med 2015;21:112-114)
김기범,나도춘,김성종,정경락 전북대학교 공학연구원 ( 구 전북대학교 공업기술연구소 ) 2003 工學硏究 Vol.34 No.-
We intended to develope an vibrating intravascular lung assist device(VIVLAD) in order to exchange of efficiently gas transfer of oxygen and carbon dioxide exchange requirements for end-stage acute respiratory distress syndrome (ARDS) patients. However, the clinical study, concluded that more gas exchange was needed for intravascular oxygenation to be clinically effective in ARDS treatment. In this study, we tried to enhance gas exchange on the VIVLAD by using microencapsulation of hemoglobin, as the microencapsulation of hemoglobin reduces blood hemolysis, and perfluorocarbon emulsion(PFC emulsion). As a result, it was shown that the oxygen transfer of hemosome and blood/hemosome mixed solution were higher than that of whole blood. Also, it showed that the carbon dioxide transfer of whole blood/PFC emulsion mixed solution was higher than that of others. Therefore, we determined that hemosome and PFC emulsion could increase oxygen transfer and carbon dioxide transfer, respectively.
헤모글로빈의 Microencapsulation에 의한 인공적혈구의 산소전달과 용혈도에 관한 연구
나도춘,김기범,김성종,정경락 전북대학교 공학연구원 ( 구 전북대학교 공업기술연구소 ) 2003 工學硏究 Vol.34 No.-
In this study, we tried to improve oxygen transfer rate and reduce pressure drop on intravascular lung assist device with microencapsulation of red blood cells, because microcapsulation of red cells reduce blood hemolysis. Hemoglobin was purified from outdated human red blood cells used. Phospholipid was purified from egg yolk. The hemosome was prepared by encapsulation of hemoglobin with phospholipid multilayer, using rotary vacuum evaporator. The function of hemosome and hemosome/blood mixed solution was tested by measuring oxygen dissociation curve using blood gas analyzer. Also we were measured about hemolysis of hemosome, hemosome/blood mixed solution and blood by absorptivity method. As a results, the oxygen dissociation curve of hemosome and hemosome/blood mixed solution showed the same figure as the curve of normal red blood cells. Also, the hemolysis of hemosome and hemosome/blood mixed solution was measured less than normal red blood cell. Therefore, the oxygen transfer rate showed high use of hemosome, and hemosome/blood mixed solution rather than normal blood. Because microen- capsulation of normal red blood cells prevent hemolysis of normal red blood cells.
Effect of happiness and depression in menopausal women based on exercise participation
Min-Gyeong Kim,Do-Hee Kim,Eun-Hye Kim,Jeong-Eun Kim,Seon-Yeong Baek,Gyeong-Suk Heo 한국운동재활학회 2014 한국운동재활학회 학술대회 Vol.2014 No.10
The purpose of this study was to investigate the effect of happiness and depression in menopausal women based on exercise participation. The subjects were 40 women, which was divided into two groups, this is, exercise group & non-exercise group to menopausal women. The attained data was analyzed the difference it by independent t-test and Levene's test. The results of the study can be attained in the followings; First, it was shown the significant, difference between exercise and non-exercise group, about the thoughts and feelings of depression. Second, it was shown the significant, difference between exercise and non-exercise group, about the activity and interpersonal of depression. Third, it was shown the significant, difference between exercise and non-exercise group, about the thoughts and feelings of happiness. Fourth, it was shown the significant, difference between exercise and non-exercise group, about the activity and interpersonal of happiness.
Kim, Young-Jon,Kim, Byoung-Ryun,Ryu, Jae-Suk,Lee, Gyeong-Ok,Kim, Hak-Ryul,Choi, Keum-Ha,Ryu, Jae-Won,Na, Kyoung-Suk,Park, Min-Cheol,So, Hong-Seob,Cho, Ji-Hyun,Park, Do-Sim Blackwell Scientific Publications 2017 International journal of gynecological cancer Vol.27 No.2
<B>Objective</B><P>Heterogeneous nuclear ribonucleoprotein A1 (HNRNPA1), serine/arginine-rich splicing factor 1 (SRSF1), and SRSF3 are splicing regulators associated with oncogenesis. However, the alterations of SF proteins and their diagnostic values in cervical cancer are unclear. To apply SFs clinically, effective marker selection and characterization of the target organ properties are essential.</P><B>Materials and Methods</B><P>We concurrently analyzed HNRNPA1, SRSF1, SRSF3, and the conventional tumor markers squamous cell carcinoma antigen (SCCA) and carcinoembryonic antigen (CEA) in cervical tissue samples (n = 127) using semiquantitative immunoblotting. In addition, we compared them with p16 (cyclin-dependent kinase inhibitor 2A [CDKN2A]), which has shown high diagnostic efficacy in immunohistochemical staining studies and has been proposed as a candidate protein for point-of-care screening biochemical tests of cervical neoplasia.</P><B>Results</B><P>HNRNPA1, higher molecular weight forms of SRSF1 (SRSF1-HMws), SRSF3, CEA, and p16 levels were higher (<I>P</I> < 0.05) in cervical carcinoma tissue samples than in nontumoral cervical tissue samples. However, the levels of SRSF1-Total (sum of SRSF1-HMws and a lower molecular weight form of SRSF1) and SCCA, a commonly used cervical tumor marker, were not different between carcinoma and nontumoral tissue samples. In paired sample comparisons, HNRNPA1 (94%) showed the highest incidence of up-regulation (carcinoma/nontumor, >1.5) in cervical carcinoma, followed by p16 (84%), SRSF1-HMws (69%), SRSF3 (66%), CEA (66 %), SCCA (32%), and SRSF1-Total (31%). HNRNPA1 (92%) and p16 (91%) presented the two highest diagnostic accuracies for cervical carcinoma, which were superior to those of SRSF3 (75%), SRSF1-HMws (72%), CEA (72%), SCCA (59%), and SRSF1-Total (55%).</P><B>Conclusions</B><P>Our results identified that HNRNPA1 is the best diagnostic marker among the SFs and conventional markers given its excellent diagnostic efficacy for cervical carcinoma, and it has a p16-comparable diagnostic value. We suggest that HNRNPA1 is an additional effective target protein for developing cervical cancer detection tools.</P>
Do-Gyeong Kim 위기관리 이론과 실천 2021 Crisisonomy Vol.17 No.7
본 연구는 재범자의 운전자 관련 특성을 기반으로 음주운전 재발 가능성에 영향을 미치는 유의한 요인을 규명하고자 하였다. 음주운전 재발이 발생할 때까지의 기간의 차이를 확인하기 위해 생존 모델이, 음주운전 재발 가능성에 영향을 미치는 요인을 규명하기 위해 Cox 비례위험 모델이 사용되 었다. 분석을 위해 도로교통공단 의정부지청에서 음주운전으로 기소된 운전자 320명을 대상으로 음주운전 특별교통안전교육 프로그램을 이수한 데이터를 수집하고, 경찰청 데이터베이스 시스템의 음주운전 관련 자료와 결합했다. 생존 분석 결과, 첫 번째 위반에서 두 번째 위반까지의 소요되는 중위수 기간은 약 347.8주인 것으로 분석되었으며, 음주운전 재발 가능성에 영향을 미치는 위험 요인 으로 연령, 성별, 직업, 교육 및 혈중 알코올 농도가 유의한 것으로 나타났다. 분석결과로부터 알 수 있듯이 운전자 인적요인별로 차별화된 교육과 관리를 위한 대책 수립이 필요한 것으로 판단된다. This study attempted to identify significant factors affecting the likelihood of Driving Under the Influence of Alcohol (DUIA) recurrence based on the drivers’ related characteristics of recidivists. A survival model was used to identify the differences in time period taken until the DUIA recurrence occurs, and a Cox proportional hazard model was employed to investigate which factors are more likely to affect the likelihood of DUIA recurrence. For the analysis, data were collected through a survey of 320 drivers who had been prosecuted as DUIA and to complete the DUIA special traffic safety education program at the Uijeongbu branch of Road Traffic Authority, and then combined with DUIA-related data stored at the National Police Agency database system. The results of survival analysis indicated that the median period from the first offence to the second one was about 347.8 weeks, and that age, gender, occupation, education, and Blood Alcohol Concentration (BAC) were significantly found as risk factors affecting the likelihood of DUIA recurrence. From the analytical results, it seems to establish a countermeasure for differentiated education and management for each driver factor.