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( Ja Young Moon ),( Won Il Kim ),( Jin Hur ),( Won Kyung Kim ),( Min Su Choi ),( Jae Sam Lim ),( Sung Bok Kim ),( Jeong Sang Cho ),( Yeong Hwan Choi ),( Byoung Joo Seo ) 한국동물위생학회(구 한국가축위생학회) 2017 韓國家畜衛生學會誌 Vol.40 No.4
Enterotoxigenic Escherichia coli (ETEC) strains producing each F4, F5, F6 and F41 fimbriae were lysed by GI24 peptide. The lysate cells were used as ETEC vaccine candidate. This study was carried out to examine whether intramuscular (im) immunization of pregnant sows with the novel vaccine candidate could effectively protect their neonatal piglets against ETEC colibacillosis. All pregnant sows were primed at 11 weeks and were boosted at 14 weeks of pregnancy. Group A sows were im inoculated with PBS. Group B sows were im immunized with 2×10<sup>9</sup> the mixture. Seral IgG, colostral IgA and IgG titers from group B sows, and seral IgG and IgA levels in group B piglets were significantly higher than those of group A sows and piglets, respectively. After challenge with wild-type ETEC, diarrhea and mortality was not observed in group B piglets. However, diarrhea was observed in 66.7% of group A piglets, and 33.3% mortality was observed. These findings indicate that im immunization of sows with the mixture of the novel vaccine candidate can effectively protect their offspring from ETEC colibacillosis.
육계에서 깃털을 이용한 플루오르퀴놀론계 잔류 모니터링 가능성 조사
김재호 ( Jae-ho Kim ),김미희 ( Mi-hee Kim ),안길호 ( Gil-ho Ahn ) 한국동물위생학회(구 한국가축위생학회) 2020 韓國家畜衛生學會誌 Vol.43 No.3
This study was conducted to analyze feasibility of monitoring of fluoroquinolnes residual through feather analysis in broilers. The calibration curve showed good linearity (r <sup>2</sup>≥0.99) within the concentration range of 1∼100 μg/kg. The limit of detection (LOD) and limit of quantification (LOQ) were validated at ≤0.66 and ≤1.99 μg/kg in broiler feather, respectively. The recoveries in feather samples ranged from 94.6 to 114.4% (5.1-15.8% RSD) at the 5 to 20 μg/kg spiking levels. The proposed new analytical method proved to be suitable and effective for fluoroquinolnes determination. We also monitored fluoroquinolones residue in 36 samples (broiler that were slaughtered in Gyonggi-do) using this method. Among tested feather samples, enrofloxacin and ciprofloxacin were detected in all samples. In muscle samples, enrofloxacin was detected in 20 (55.6%) samples and ciprofloxacin was not detected.
정광 ( Kwang Jung ) 한국동물위생학회(구 한국가축위생학회) 2020 韓國家畜衛生學會誌 Vol.43 No.3
This study was performed to determine the seroprevalence of reticuloendotheliosis virus (REV) infection in layer chickens in Gyeonggi province, South Korea. Serum samples were collected from 820 layer chickens on 56 flocks in Gyeonggi province. The samples were tested for specific antibodies against REV using commercial enzyme-linked immunosorbent assay (ELISA). The seroprevalence of REV antibodies in the flocks was 62.50% (95% Confidence interval (CI) 49.33∼74.40) and the overall seroprevalence in individual chickens was 39.27% (95% CI 35.97∼42.65). The results of the present survey indicate that REV infection is prevalent in layer chickens in Gyeonggi province, South Korea. Therefore, effective measures should be taken to prevent and control REV of layer chickens in this province.
소 결핵 진단을 위한 인터페론감마 검사 키트의 성능 비교 평가
홍이곤 ( Leegon Hong ),최우재 ( Woojae Choi ),노영혜 ( Younghye Ro ),안선민 ( Sunmin Ahn ),김은경 ( Eunkyung Kim ),최은희 ( Eunhee Choe ),김단일 ( Danil Kim ) 한국동물위생학회(구 한국가축위생학회) 2020 韓國家畜衛生學會誌 Vol.43 No.4
In Korea, bovine tuberculosis (bTB) is a representative zoonotic disease that causes considerable economic loss. In determining the positive bTB, the ELISA method for examining the amount of interferon- gamma (IFN-γ) is included in Korea’s diagnostic standard method. Recently, commercially available BIONOTE TB-Feron ELISA Plus (TB-Feron Plus) that detects IFN-γ has been introduced. However, since the scientific basis for the performance is limited, we evaluated performance by comparing it with the results of another IFN-γ ELISA assay kit (BOVIGAM <sup>Ⓡ</sup> ) certified by Office International des Epizooties. In our research, 42 positive blood samples preliminarily tested with a tuberculin skin test and/or BOVIGAM <sup>Ⓡ</sup> and 54 negative blood samples collected from three bTB free farms were subjected to IFN-γ assay using the TB-Feron Plus and the BOVIGAM <sup>Ⓡ</sup> , respectively. The result shows that the sensitivity, specificity and accuracy were 81.0% (34/42), 100% (54/54), 91.7% (88/96) in TB-Feron Plus kit and 78.6% (33/42), 100% (54/54), 90.6% (87/96) in BOVIGAM <sup>Ⓡ</sup> kit, respectively. Moreover, the overall accordance percentage of the two kits was 99.0% (95/96) and there was almost perfect agreement between two assays (Kappa=0.977, P<0.0001). Furthermore, additional studies confirmed that elevated lymphocyte numbers in blood did not interfere with the results of the TB-Feron Plus kit. And, delayed time from sampling to culture decreased the optical density (OD) value. Therefore, we concluded that the TBFeron Plus kit was not inferior to BOVIGAM <sup>Ⓡ</sup> in performance. High lymphocyte numbers in blood did not impact on TB-Feron Plus results, while delayed time before culture interfered with OD value.
성민호 ( Min-ho Seong ),박종식 ( Jong-sik Bak ),윤도경 ( Do-kyung Youn ),김형수 ( Hyeong-su Kim ),고병효 ( Byeong-hyo Ko ),함정민 ( Jeong-min Ham ),정명호 ( Myeong-ho Jeong ) 한국동물위생학회(구 한국가축위생학회) 2020 韓國家畜衛生學會誌 Vol.43 No.4
Coxiella burnetii is the causative agent of Q fever which is a zoonosis occuring in both humans and animals worldwide. The purpose of this study was to investigate the seroprevalence of C. burnetii in Korean native goat in Gyeongnam province, Korea. A total of 1,365 goat blood samples from 273 farms in Gyeongnam province were collected between 2018 and 2019. Among them, 177 (13.0%) samples out of 71 (26.0%) farms were seropositive for C. burnetii by ELISA. Seroprevalence were 15.4% and 10.9% in 2018 and 2019, respectively. According to the region, seroprevalence in western, central, eastern, northern and southern areas of Gyeongnam province were 16.6%, 17.8%, 8.0%, 11.6% and 10.8%, respectively. Seroprevalence was increased with breeding scale (Head<10:7.0%, 10≤Head<50:8.7%, 50≤Head<100:13.6%, 100≤Head:28.8%). Seroprevalence according to the season showed highest in summer (18.9%) and lowest in winter (9.4%). These results indicated that C. burnetii infection is widespread among Korean native goats of Gyeongnam province in Korea and further study needs to prevent the circulation of other livestock with Korean native goat.
고재형 ( Jae-hyung Ko ),박미영 ( Mi-young Park ),신병훈 ( Byung-hoon Shin ),남영희 ( Young-hee Nam ),구경녀 ( Kyung-nyer Ku ),손주일 ( Ju-ill Son ) 한국동물위생학회(구 한국가축위생학회) 2020 韓國家畜衛生學會誌 Vol.43 No.4
This study was conducted to survey pathogens of canine coronavirus (CCV), canine distemper virus (CDV), canine influenza virus (CIV), canine parvovirus (CPV), severe fever with thrombocytopenia syndrome virus (SFTSV), Dirofilaria (D.) immitis, Giardia and antibodies against Anaplasma (A.) phagocytophilum, Borrelia (B.) burgdorferi, Brucella (B.) canis and Ehrlichia (E.) canis among stray dogs in Gyeonggi province. We collected 271 feces, 291 bloods, 311 nasal and ocular swab samples from 311 of dogs in the Gyeonggi province assistance dogs sharing center from January to December, 2019. Among canine infectious disease pathogens, Giardia was highly detected in 46/271 (17.0%) samples. Subsequently, CCV 10.3% (28/271), D. immitis 8.2% (24/291), CPV 4.1% (11/271), CDV 1.0% (3/311), A. phagocytophilum (antibody) 0.3% (1/291), E. canis (antibody) 0.3% (1/291) were detected. Based on the results, this study is expected to provide a useful reference for disease control and management of stray dogs.
Accidental electrocution in two cattle confined in stanchions
( Su Gwon Roh ),( Jongho Kim ),( Jae-cheong Cho ),( Eun-jin Choi ),( Byungjae So ),( Kyunghyun Lee ) 한국동물위생학회(구 한국가축위생학회) 2020 韓國家畜衛生學會誌 Vol.43 No.3
On a farm with 6 Korean beef cattle, an adult cow and a calf suddenly died while eating. At that time, hair was pressed upon the stanchion facilities where the adult cow was fed. Two dead cattle were submitted to the Animal and Plant Quarantine Agency for disease diagnosis. Grossly, the mouth of the adult cow was full of feed that had been eaten before death, and there was a foamy fluid in the calf’s trachea. Both hemorrhages were observed in the muscles, as well as in the internal organs such as the liver, lungs, and heart. Ruminal mucosa was easily peeled off. Histopathologically, both muscles and internal organs showed severe hemorrhages. In particular, affected muscles were shown segmental and band-like patterns of myonecrosis, disappeared some myocytes, burned hair follicles, and irregular surface of skin. Severe hemorrhages of muscles and internal organs were consistent with previous reports. Easily separated mucosa of rumen and muscle lesions were very characteristic. We determined electrocution according to the pathological findings, laboratory results, and traces of accidents. This report will help to understand of pathological lesions of electrocution. Furthermore, this case suggests that the electrical wires and devices must be installed and handled carefully during the construction of the cowsheds care.
PRRS 양성농장의 사육단계별 구강액과 혈액을 이용한 PRRSV와 항체 검출 비교
김정희 ( Jung-hee Kim ),손재국 ( Jae Guk Son ),김원일 ( Won-il Kim ) 한국동물위생학회(구 한국가축위생학회) 2020 韓國家畜衛生學會誌 Vol.43 No.3
The objective of this study was to evaluate the usefulness of detection of PRRSV and PRRSV-specific antibodies in oral fluids for monitoring of PRRSV infection in endemic farms. The level of PRRSV and anti-PRRSV antibodies in serum and oral fluids was evaluated in five age groups of pigs (6, 9, 12, 16 weeks of age and gilts). The samples (25 serums and 5 oral fluids/per a farm) were collected from 5 different farms endemically infected by PRRSV. Both serum and oral fluid samples were tested for PRRSV by quantitative reverse-transcription polymerase chain reaction (qRT-PCR) and for anti-PRRSV antibodies by two commercial PRRSV ELISA kits. ELISA mean s/p ratios (2.98 vs 1.63) and positive rate (84.0% vs 68.8%) of the oral fluid samples showed significantly higher levels but had similar patterns to the seroprofile of the blood samples. The PRRSV positive rate of oral fluid and serum samples was 40.0% and 44.0% respectively. In conclusion, the use of oral fluids for PRRS monitoring in endemic farms is strongly recommended.
국내 가금 농장에서 야생조류 침입 억제 장치를 이용한 야생조류 출현율 조사 및 평가
김용식 ( Youngsik Kim ),김윤지 ( Yunji Kim ),나은지 ( Eun-jee Na ),이숙영 ( Sook-young Lee ),엄재구 ( Jae-ku Oem ) 한국동물위생학회(구 한국가축위생학회) 2020 韓國家畜衛生學會誌 Vol.43 No.3
The epidemiological investigation of outbreak in korea confirmed that the inflow of avian influenza (AI) is related to the migration of migratory birds. In this study, avian repellents instrument were implemented and developed using the visual effects of lasers in accordance with the situation of small domestic fowl farms, and monitoring cameras were installed around each instrument to investigate the frequency of wild birds appearing and evaluate the performance of the instrument. Observation showed that the appreance ratio was reduced by 95%, and no significant reduction in the intrusion prevention effect by adaptation was observed on all fowl farms. In conclusion, it is expected that the outbreak of wild bird-borne infectious diseases such as avian influenza will be decreased if the device is installed on domestic fowl farms.
Analysis of hematological changes in normal and diarrhea calves
( Ru-hui Song ),( Jin-hee Kang ),( Kwang-man Park ),( Jung-ho Youm ),( Jin-ho Park ) 한국동물위생학회(구 한국가축위생학회) 2020 韓國家畜衛生學會誌 Vol.43 No.3
In order to effective treats a sick calf, it is necessary to obtain accurate information about the patient’s condition. However, the standard references for Korean cattle claves are not well known. Therefore, this study aims to present useful clinical values by analyzing normal blood and diarrhea blood of Hanwoo calf. Recently, with the advent of a portable blood analyzer, it is possible to immediately analyze the patient’s condition and severity in the field, not in the laboratory, and to calculate a suitable dosage for supporting fluid therapy. Therefore, in this study, the distribution of red blood cells (RBC) and white blood cells (WBC) were analyzed in normal and diarrhea Hanwoo calves. As a result, Hematocrit (HCT) levels increase significantly between 1 and 20 days in diarrhea positive calves. Changes in leukocyte composition had similar growth patterns in normal and diarrhea calves. As it grew, the proportion of neutrophils decreased and lymphocytes increased. However, the number of WBCs increased from 1 to 10 and 21 to 30 days in diarrhea positive calves, which is closely related to the increase in neutrophils. Therefore, those data can be used for diagnosis and treatment of diarrhea calf.