m6A-mediated upregulation of AC008 promotes osteoarthritis progression through the miR-328-3p‒AQP1/ANKH axis

Yang Jiashu,Zhang Ming,Yang Dawei,Ma Yunfei,Tang Yuting,Xing Mengying,Li Lingyun,Chen Li,Jin Yucui,Ma Changyan 생화학분자생물학회 2021 Experimental and molecular medicine Vol.53 No.-

Long noncoding RNAs (lncRNAs) have emerged as important regulators of osteoarthritis (OA), but the biological roles and clinical significance of most lncRNAs in OA are not fully understood. Microarray analysis was performed to identify differentially expressed lncRNAs, mRNAs, and miRNAs between normal and osteoarthritic cartilage. We found that AC008440.5 (abbreviated AC008), as well as AQP1 and ANKH, were highly expressed in osteoarthritic cartilage, whereas miR-328-3p was expressed at a low level in osteoarthritic cartilage. Functional assays showed that ectopic expression of AC008, AQP1, and ANKH significantly decreased chondrocyte viability and promoted chondrocyte apoptosis and extracellular matrix (ECM) degradation, whereas knockdown of AC008, AQP1, and ANKH resulted in the opposite effects. Moreover, miR-328-3p overexpression increased chondrocyte viability and attenuated chondrocyte apoptosis and ECM degradation, whereas inhibition of miR-328-3p resulted in the opposite effects. Bioinformatics analysis, RNA immunoprecipitation (RIP), and luciferase assays revealed that AC008 functioned as a competing endogenous RNA (ceRNA) to regulate miR-328-3p, which specifically targeted the AQP1 and ANKH genes. In addition, miR-328-3p significantly ameliorated MIA-induced OA, whereas AC008 accelerated OA progression in vivo. Furthermore, fat mass and obesity-associated (FTO)-mediated N6-methyladenosine demethylation downregulated AC008 transcription, while lower FTO expression led to upregulation of AC008 transcription in OA. In conclusion, our data reveal that AC008 plays a critical role in OA pathogenesis via the miR-328-3p‒AQP1/ANKH pathway, suggesting that AC008 may be a potential therapeutic target for OA.

Influence of oxygen vacancies on the EPIR effect in Nd0.7Sr0.3MnO3 ceramics

Dawei Shi,Shunsheng Chen,Di Liu,Lingfang Xu,Haibo Xiao,Changping Yang,Kaiying Wang 한국물리학회 2015 Current Applied Physics Vol.15 No.8

Nd0.7Sr0.3MnO3 polycrystalline ceramic samples (NSMO) were synthesized by solid state reaction with a post annealing for parts of samples under different partial pressures of oxygen at 1350 ℃ for 12 h. XRD shows that all the samples are in single phase with a perovskite structure. The electrical transport measurement implies that the oxygen concentration has an important influence on the electrical pulse induced resistance, i.e. the EPIR-effect, for NSMO samples. For the oxygen-enriched sample, which probably contains a smaller amount of oxygen vacancies, the I-V characteristic is linear with a very small resistance, and the EPIR-effect is not detectable within the experimental accuracy. However, after sintered and annealed in reducing atmospheres, an EPIR effect appears and even becomes stronger and more stable if the sintering atmosphere is more reducing than that of normal air condition. Besides, the I -V becomes nonlinear and the initial resistance increases with increasing deficiency of oxygen, which means that the oxygen vacancies and their deep level trap states play an important role for the EPIR effect of NSMO. In this manuscript, an oxygen vacancy trapped state model is put forward to explain the relation between oxygen concentration and the EPIR effect.

Flow patterns of solid in water in oil (S/W/O) compound droplets formation in a microfluidic device with perpendicular shear

Dawei Pan,Qiang Chen,Lan Xu,Can Yang,Meifang Liu,Weixing Huang,Bo Li 한국공업화학회 2019 Journal of Industrial and Engineering Chemistry Vol.75 No.-

Theflow patterns and corresponding breakup dynamics of solid in water in oil compound droplets underperpendicular shear force were experimentally investigated in a microfluidic device. Three typicalflowpatterns, namely double-encapsulation, plugs, and cobbles, were found and be quantitativelydistinguished by the capillary number (Cac) of continuous phase and theflow rate ratio (R) ofcontinuous phase to dispersed phase. It is found that, under all offlow regimes, the consecutive entry ofsolid particles leads d1 (the neck thickness along the center of the side channel) varying periodically. Inthe double-encapsulation regime, the neck thickness (d2) between thefirst and second particle increasesas the interface deformation recovers driven by the interfacial tension force, whereas the neck thickness(d3) between the second and third particle decreases to break off because the neck has been stretchedlong to become thin enough. As for the cobbles regime, hydrostatic pressure difference still remainsdominant, but not a shearing force. Specially, different from the W/O droplet formation, it suggests thatvarious breakup dynamics are always caused by a non-free deformation of S/W interface and the largediameter of solid particle. Finally, theflow pattern diagrams dependent on Cac and R are obtained.

A New FE Modeling Method for Isothermal Local Loading Process of Large-scale Complex Titanium Alloy Components Based on DEFORM-3D

Dawei Zhang,He Yang,Zhichao Sun,Xiaoguang Fan 한국소성가공학회 2010 기타자료 Vol.2010 No.6

Isothermal local loading process provides a new way to form large-scale complex titanium alloy components. The forming process is characterized by an extreme size (large scale in global and compared small size in regional), multi-parameter effects, and complicated loading path. To establish a reasonable finite element model is one of the key problems urgently to be solved in the research and development of isothermal local loading forming process of largescale complex titanium alloy components. In this paper, a new finite element model of the isothermal local loading process is developed under the DEFORM-3D environment based on the solution of some key techniques. The modeling method has the following features: (1) different meshing techniques are used in different loading areas and the number of meshed elements is determined according to the deformation characteristic in different local loading steps in order to improve computational efficiency; (2) the accurate magnitude of the friction factor under titanium alloy hot forming (isothermal forming) condition is adopted instead of the typical value for lubricated hot forming processes; (3) different FEM solvers are chosen at different stages according to the loading characteristic and the contact state; (4) in contrast to the local component model, a full 3D component is modeled to simulate the process. The 3D-FE model is validated by experimental data of a large-scale bulkhead forming under isothermal local loading. The model can describe the quantitative relationships between the forming conditions and the forming results. The results of the present study may provide a basis for studying the local deformation mechanism, selecting the reasonable parameters, optimizing the die design and the process control in isothermal local loading process of large-scale complex titanium alloy components.

Research on Corn Region Extraction of Remote Sensing Image Based on Fusion of PSO Parameters Optimization and Adaboost_SVM

Dawei Li,Fengbao Yang,Hong Wei 보안공학연구지원센터 2015 International Journal of Signal Processing, Image Vol.8 No.8

Study of Visual Spatial Perception Performance at Close Range in Underground Coal Mine

Ren Dawei,Liu Yang,Chen Litao 보안공학연구지원센터 2015 International Journal of Signal Processing, Image Vol.8 No.11

In coal mines the environment is complex, crowded with personnel and equipment, as well as lack of colors. The safety in production is influenced by visual spatial perception is whether or not accurate. The visual spatial perception precision plays a significant even crucial role in positioning. By simulating the environment in coal mines, the positioning experiment about participants’ visual spatial perception performance of different target objects in two models—from far to near and from near to far has been carried out. Experimental results showed that visual spatial perception distance was significantly shorter than target range, that visual spatial perception in deep pink was the poorest. In addition, the H has become appreciable and regular with visual spatial perception changing. The experimental results provided references for setting warning marks, safe distances and choosing colors for infrastructure in coal mines.

Laccase Biosensor Based on Ag-Doped TiO2 Nanoparticles on CuCNFs for the Determination of Hydroquinone

Jie Yang,Dawei Li,Zengyuan Pang,Qufu Wei 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2016 NANO Vol.11 No.12

A novel nanomaterial composed of copper and carbon nanofibers (CuCNFs) decorated with Agdoped TiO2 (Ag–TiO2) nanoparticles was prepared through electrospinning, carbonization and solvothermal treatment. The composites were characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR) and electrochemical impedance spectroscopy (EIS). The obtained composites were mixed with laccase and Nafion to construct novel hydroquinone biosensor. The electrochemical behavior of the novel biosensor was studied using cyclic voltammetry (CV) and chronoamperometry. The results demonstrated that the biosensor possessed a wide detection linear range (1.20–176.50 µM), a good selectivity, repeatability, reproducibility and storage stability. This work provides a new material to design more efficient laccase (Lac) based biosensor for hydroquinone detection.

Construction of E-Commerce Entrepreneurship Curriculum System for College Students: An Information Entrepreneurship Perspective

Yan Hou,Dawei Liu,Shuling Yang 보안공학연구지원센터 2016 International Journal of u- and e- Service, Scienc Vol.9 No.11

With the rapid development of Electronic Commerce, the demand for the quantity and professional knowledge of the professional talents in electronic commerce is continuously improving. The development of network market provides an opportunity for college students to start their own business, and the ultimate goal of e-commerce business entrepreneurship is to cultivate students' ability to use e-commerce to start a business. In this paper, the authors analyze the construction of e-commerce entrepreneurship curriculum system for college students based on information technology perspective. In the curriculum system construction, the course is divided into three levels; the first level is mainly about the basic knowledge of e-business, second level is mainly about business development course, and the third levels of curriculum mainly about laws and regulations. Establish and perfect the innovation and entrepreneurship curriculum system, and cultivate the students' awareness of online entrepreneurship is a development trend in the future.

Graphene Oxide/Polyester Fabric Composite by Electrostatic Self-Assembly as a New Recyclable Adsorbent for the Removal of Methylene Blue

Di Wang,Dawei Li,Pengfei Lv,Qingqing Wang,Yang Xu,Qufu Wei 한국섬유공학회 2018 Fibers and polymers Vol.19 No.8

A novel graphene oxide/polyester (GO/PET) composite fabric as a recyclable adsorbent was prepared via electrostatic self-assembly. The structure, morphology, and properties of the GO/PET composite fabrics were characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM), atomic force microscopy (AFM), Fourier transformed infrared spectroscopy (FT-IR), thermogravimetric analysis (TGA), and contact angle (CA), respectively. The absorption property was evaluated by the absorption amount and removal efficiency of methylene blue (MB) solution on the GO/PET composite fabric. The results indicated that the absorption amount was found to be 21.80 mg/g and the removal efficiency reached 99.93 % under the experimental conditions of GO concentration of 2 mg/ml, initial concentration of 50 mg/l, and area of 64 cm2. The experimental parameters were investigated including the concentration of GO, the initial concentration of MB solutions, and adsorbent area. Simultaneously, according to a series of dynamic analysis, the absorption process revealed that the kinetics was well-described by pseudo-second-order model. This study showed that the GO/PET composite fabric could be a recyclable, efficient adsorbent material for the environmental cleanup.

Preparation and Investigation of Ulex Europaeus Agglutinin IConjugated Liposomes as Potential Oral Vaccine Carriers

KeXin Li,DaWei Chen,XiuLi Zhao,HaiYang Hu,ChunRong Yang,DaHai Pang 대한약학회 2011 Archives of Pharmacal Research Vol.34 No.11

We prepared and optimized Ulex europaeus agglutinin I (UEAI)-modified Bovine serum albumin (BSA)-encapsulating liposomes (UEAI-LIP) as oral vaccine carriers and examined the feasibility of inducing systemic and mucosal immune responses by oral administration of UEAILIP. The prepared systems were characterized in vitro for their average size, zeta potential,encapsulation efficiency (EE%) and conjugation efficiency (CE%). In vitro release studies indicated that the presence of UEAI around the optimized liposomes was able to prevent a burst release of loaded BSA and provide sustained release of the encapsulated protein. In vivo immune-stimulating results in KM mice showed that BSA given intramuscularly generated systemic response only but both systemic and mucosal immune responses could be induced simultaneously in the groups in which BSA-loaded liposomes (LIP) and UEAI-LIP were administered intragastrically. Furthermore, the modification of UEAI on the surface of liposomes could further enhance the IgA and IgG levels obviously. In conclusion, this study demonstrated the high potential of lectin-modified liposomes containing the antigen as carriers for oral vaccine.