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      • Mush-room의 生産性에 관한 硏究

        鄭大敎 건국대학교 1968 學術誌 Vol.9 No.1

        Studies were made to determine the factor of the decrease Korean mush-room production by spawn. Following are the results obtained. ① Used spawn are DA, DB, MM, CA, CB, FN, KA, KB, SY and KS. ② Used media are soybean sauce, corn, potato, malt, pettone, glucose, wood destroying fungi (II) and wood destroying fugni agar media (III). ③ Comparing the effective growing activities of spawn throw used all agar media by orders the group of SY, DA, KA, are best, the group of FN, CA, KB, MN are medium and the group of CB, KS, DB are very poor. ④ Through the all used spawn, better media are found glucose, soybean sauce, lepton, potato, agar media by order. And other media are very poor.

      • SCOPUSKCI등재

        항생제 다제내성균 Staphylococcus aureus SA2로부터 분리한 테트라사이클린 내성 플라스미드 pKH6의 염기서열

        이대운,윤성준,김우구,신철교,임성환,이백락,문경호 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.4

        한국에서 임산균주로부터 분리한 테트라사이클린(Tc) 내성 플라스미드 pKH6의 전체 염기서열을 결정하여 가장 대표적인 Tc 내성 플라스미드인 pT181과 비교하였다. pKH6의 전체 길이는 4439bp로 pT181과 동일하게 나타났으며 염기서열은 7개의 염기에서 차이를 보였다. 전부 다 염기쌍이 치환된 형태로 나타났으며 그 중 3개는 coding 부위에서 일어났다. coding 부위에서 일어난 경우에도 1개는 동일한 아미노산을 coding 하였기 때문에 pT181과 차이가 없었으며 RepC와 Pre 단백질에 있어 각각 1개의 아미노산에서 차이를 나타내었다. The complete nucleotide sequence of pKH6, a tetracycline-resistance (Tc^r) plasmid isolated from multi-drug resistant Staphlococcus aureus SA2, has been determined and compared with that of the staphylococcal Tc^r plasmid pT181. The nucleotide sequences of the two plasmids are in agreement except for 7 nucleotides. All differences are caused by base pair substitutions. Among 6 substitutions, 3 occurred in coding regions. However, only tow base substitutions in coding regions resulted in changes of amino acid sequences in two different ORFs of repC and Pre proteins.

      • 핵저지능이 열자극전류에 미치는 영향

        邊大鉉,趙昌熙,韓斗敎 弘益大學校 1987 弘大論叢 Vol.19 No.2

        In this study induced thermally stimulated current by the TSC was measured by the open circuit method after implanting Na+ ion into a PET film. With the result, using a new theory, activated energy, escape frequency, relaxation time and ion density was evaluated. (-) peak appeared at 363 K and 390 K when temperature increasing rate was 0.1 K/sec. and a (+) peak in the range 310~350 K. As a result of analysis, the peak at 363 K was by Na^(+) ion trapped in the surface trap and the activated energy 1. 14eV. 390 K peak had 1.66 eV activated energy and was by Na+ ion trapped in a vacance. The TSC current of 390 K peak was proportional to nuclear stopping power.

      • 病原性 大腸菌이 생성하는 耐熱性 腸毒素의 抗體 생성조건 및 中和效果

        金敎昌,都大洪,鄭埈永 충북대학교 농업과학기술연구소 1993 農業科學硏究 Vol.11 No.1

        The heat-stable enterotoxin(ST) producing Escherichia coli strains were isolated from the swine. The purified ST was immunized with ST-BSA conjugate and obtained antiserum from a rabbit (New Zealand Whit). A pathogenic toidity was neutralized with anti-rabbit serum. When antiserum obtained from rabbits immunized with ST- BSA antigens. It was production the most of antiserum at the fourth immunization (21 days). Obatined antisera neutralized the toxic activity of purified 4 mouse units to 1 : 32 of antisera dilution folds.

      • KCI등재

        분꽃씨의 抗알레르기 效果에 대한 硏究

        鄭大琪,宋昊埈,辛民敎 대한본초학회 1997 大韓本草學會誌 Vol.12 No.1

        This study was investigated the inhibitory activity of Semen Mirabilis (SM) (Nyctaginaceae) on type 1 immediate hypersensitivity of the anaphylactic type. 1 used a mouse model of passive cutaneous anaphylaxis, an IgE-mediated, mast cell-dependent reaction. Administered orally at 250, 500 ㎎/㎏ body weight 1 h before the challenge, SM was potently inhibited PCA in mouses in which disodium cromoglycate showed poor inhibitory activity. SM inhibited compound 48/80-induced anaphylaxis 94.21% with a dose of 0.5g/㎏ body weight at 1 h before or 5 and 10 min after injection of compound 48/80. SM (0.03-2.0 ㎎/㎖) also exhibited the dose-dependent inhibitory effect on compound 48/80-induced histamine release from rat peritoneal mast cells. Moreover, it was clearly demonstrated that SM and disodium cromoglycate disodium cromoglycate potently inhibited such type I allergic reactions as anaphylactic shocks, suggesting that these drugs, at least in part, share the same mechanism of action. It is suggested that SM may exert a stronger inhibition on the mast cell degranulation process. When I examined the effect of SM on the release of IgE in mouse spleen whole cells and human mycloma U266B1, the release of IgE is inhibited by Mirabilis jalapa L. These results suggest the possibility that the inhibition of allergic reaction by SM is regulated by histamine and IgE.

      • 돼지에서 由來한 耐熱性 腸毒素生産 大陽菌의 分離 및 判定

        都大洪,金敎昌,金道榮 충북대학교 농업과학기술연구소 1987 農業科學硏究 Vol.5 No.2

        Enterotoxigenic E.coli is the one of major causative occured to the infantile swine diarrhea. A total of 189 isolated idem assumed E. coli, Salmonella and Shigella was isolated from swine in Kyong-Gi and Chung-Cheong province. When enterotoxigenic E. coli was infected to infant swine, these adhered to the mucosa of small intestine with pili and excrete enterotoxin, which caused the diarrhea. The detection of a heat-stable enterotoxin(ST) produced of isolated E.coli was porformed by infant mouse assay, and was identified pili by the aggutination test of rbc suspention. The plasmid DNA profile was investigated by the agarose gel electrophoresis. The results of assay to enterotoxigenic E.cofi summarized as follow. Isolation ratio of enterotoxigenic E.coli among total of 45 isolated strains was taken 56.52% during summer. Four strains(KS-4, KM-7, KM-12, CM-7) of high produced ST were showed the G/B ratio above 1.10 by infant mouse assay. Four strains of high produced enterotoxigenic E.coli was isolated from swine with diarrhea, and 3 strains (KS-4, KM-7, KM-12) were proved to possess K88 and K99 antigens, or K88 antigen. The patters of plasmid DNA was contained 4.5kb plasmids encoded for ST. The antibiotics sensitivity patters of cultured enterotoxigenic E.coli were showed a tendency to be resistant against tetracycline and streptomycin.

      • 薄板의 加工性에 미치는 異方性에 관한 硏究(Ⅰ)

        徐大敎,金東哲,禹鎬吉 충남대학교 공업교육연구소 1981 論文集 Vol.4 No.1

        This study is concerned with the anisotropy which affects the workability of several different sheet metals. To do this, authors studied the earing through the deep drawing process, using the results which were obtained by studying the effect of the anisotropy of sheet metal in tensile yielding. The results through this study are as follows; 1) The corresponding maximum errors of the theoretical and experimental results in steel, brass, and aluminum plates in tensile yielding are 16%, 6%, and 5% respectively. 2) The earing of steel, brass and copper plates whose thickness are 0.62㎜ are two or three times as high as those of corresponding plates whose thickness are 0.34㎜. 3) The assumption of costa is satisfied in steel plate, but it is not satisfied in brass plate. It is hoped that the results of this study will be made reference to other works of sheet metal.

      • 數種 木材腐朽菌의 菌絲 發育에 미치는 防腐劑의 效果

        鄭大敎 건국대학교 1967 學術誌 Vol.8 No.1

        (1) The sterilizing effects of NaF, CuSO4, CuCO3, Hgcl2, ZnSO4, and Coaltar Creosote on the mycelial growth of Irpex Censors Berk Polystictus Versicolor L. Fr, Polystictus Versicolor L. Var Nigricans, and Schigophyllum Commune Fr. were studied. (2) The range of minimum density of the fungicides to check. The growth of four fungi mentioned above was as follow : NaF 0.15∼0.25(%) CuCO3 0.40∼0.50(%) ZnSO4 0.40∼0.45(%) CuSO4 0.20∼0.35(%) Hgcl2 0.05(%) Creosote 0.10∼0.15(5) of the fungicides tested, Hgcl2 was most effective in fungicidal effects, and Creosote, NaF, CuSO4, ZnSO4, CuCO3, followed. The order of resistance of the fungi to the fungicides was as follows: S, Commune Fr, P, Versicolor L. Var, nigricans I, Censors Berk P, Versicolor L. Fr (3) The fungicides were added to the Pepton-ager culture medium at the concentration between 0.01 and 0.5%, and the medium was filled into 9cm Petridishes, two square millimeter ager blocks prepaered separately from the fungi contained ager were placed in the middle of the Petri-dishes, incubated six days at 26℃. Diameter of biggest colonies were measured.

      • KCI등재

        韓國産 가지과 植物에 관한 本草學的 硏究

        정대기,변종호,신민교,송호준 대한본초학회 1998 大韓本草學會誌 Vol.13 No.1

        For the purpose of developing Korean Herbalogy with the plants of Solanceae which grow wild and is planted in Korea, the these and writings on herbalogy, from literature of successive generations, have been throughly investigative and the results obtained were as follows: 1. There were totaled to 13 genera and 26 species in Korea and among them medicinal plants are 11 genera, 19 species, some 73% in total but, the number of species may be added because of the similiar plants. 2. According to the oriental names which can be used for medical purpose, the medicinal plants belonging to the Solanaceae family were classified as Radix 8, Fructus 8, Herba 6 ,Foilum 5, Semen 3, Lignum and Flos 2. 3. According to sum of 26 species in Solanaceae family, they were classified into Solanum genera 8, Physalis and Capsicum genera 3 each, Datura and Nicotiana genera 2 each. Thus it was noticed that Solanum genera was the main kind, some 30% in total. 4. According to the number of species of the origin plants about each chinese materia medicals, they were calssified into HERBA SOLANI LYRATI 3, FRUCTUS CAPSICI and FLOS DATURAE 2 each. 5. According to nature and flavour of medicinal plants, they were classified into cold, cool; 17 each,warmth,heat; 8 each, balance 5 and unidentified 6. Thus it was noticed that cold and cool is the main in nature and flavour of medicinal plants. 6. According to the Properties and Principal curative action, they were classified into Drugs for treating febrifugal and detoxicant 14, Drugs for relieving pain 7, Antitussives . Diuretics . Drugs for invigorating blood circulation. Tonics 4 each, Anthelmintics 3. 7. Comparing to whole medicinal plants 19 kinds, toxic drugs include minor toxin were 3 kinds, 16% of the whole. From this result, It was revealed that the plants for medical purpose in Solanaceae was 73% of the whole, in which RADIX and FRUCTUS were abundunt. It is considered that many experiments and clinical approaches must be continued to use Solanaceae plants widely.

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