Plasma Macrophage Migration Inhibitory Factor and CCL3 as Potential Biomarkers for Distinguishing Patients with Nasopharyngeal Carcinoma from High-Risk Individuals Who Have Positive Epstein-Barr Virus Capsid Antigen-Specific IgA

Ning Xue,Jian-Hua Lin,Shan Xing,Dan Liu,Shi-Bing Li,Yan-Zhen Lai,Xue-Ping Wang,Min-Jie Mao,Qian Zhong,Mu-Sheng Zeng,Wan-Li Liu 대한암학회 2019 Cancer Research and Treatment Vol.51 No.1

Purpose The purpose of this study was to identify novel plasma biomarkers for distinguishing nasopharyngeal carcinoma (NPC) patients from healthy individuals who have positive Epstein-Barr virus (EBV) viral capsid antigen (VCA-IgA). Materials and Methods One hundred seventy-four plasma cytokines were analyzed by a Cytokine Array in eight healthy individuals with positive EBV VCA-IgA and eight patients with NPC. Real-time polymerase chain reaction, Western blotting, enzyme-linked immunosorbent assay (ELISA), and immunohistochemistry were employed to detect the expression levels of macrophage migration inhibitory factor (MIF) and CC chemokine ligand 3 (CCL3) in NPC cell lines and tumor tissues. Plasma MIF and CCL3 were measured by ELISA in 138 NPC patients, 127 EBV VCA-IgA negative (VN) and 100 EBV VCA-IgA positive healthy donors (VP). Plasma EBV VCA-IgA was determined by immunoenzymatic techniques. Results Thirty-four of the 174 cytokines varied significantly between the VP and NPC group. Plasma MIF and CCL3 were significantly elevated in NPC patients compared with VN and VP. Combination of MIF and CCL3 could be used for the differential diagnosis of NPC from VN cohort (area under the curve [AUC], 0.913; sensitivity, 90.00%; specificity, 80.30%), and combination of MIF, CCL3, and VCA-IgA could be used for the differential diagnosis of NPC from VP cohort (AUC, 0.920; sensitivity, 90.00%; specificity, 84.00%), from (VN+VP) cohort (AUC, 0.961; sensitivity, 90.00%; specificity, 92.00%). Overexpressions of MIF and CCL3 were observed in NPC plasma, NPC cell lines and NPC tissues. Conclusion Plasma MIF, CCL3, and VCA-IgA combination significantly improves the diagnostic specificity of NPC in high-risk individuals.

Optimized Medium Improves Expression and Secretion of Extremely Thermostable Bacterial Xylanase, XynB, in Kluyveromyces Lactis

( Yin Tie ),( Li Li Miao ),( Fei Fei Guan ),( Gui Li Wang ),( Qing Peng ),( Bing Xue Li ),( Guo Hua Guan ),( Ying Li ) 한국미생물 · 생명공학회 2010 Journal of microbiology and biotechnology Vol.20 No.11

An extremely thermostable xylanase gene, xynB, from the hyperthermophilic bacterium Thermotoga maritima MSB8 was successful expressed in Kluyveromyces lactis. The response surface methodology (RSM) was also applied to optimize the medium components for the production of XynB secreted by the recombinant K. lactis. The secretion level (102 mg/l) and enzyme activity (49 U/ml) of XynB in the optimized medium (yeast extract, lactose, and urea; YLU) were much higher than those (56 mg/l, 16 U/ml) in the original medium (yeast extract, lactose, and peptone; YLP). The secretory efficiency of mature XynB was also improved when using the YLU medium. When the mRNA levels of 13 characterized secretion-related genes in the K. lactis cultured in YLP and YLU were detected using a semiquantitative RT-PCR method, the unfolded protein response (UPR)-related genes, including ero1, hac1, and kar2, were found to be up-regulated in the K. lactis cultured in YLU. Therefore, the nutrient ingredients, especially the nitrogen source, were shown to have a significant influence on the XynB secretory efficiency of the host K. lactis.

A Color-Reaction-Based Biochip Detection Assay for RIF and INH Resistance of Clinical Mycobacterial Specimens

( Wen Fei Xue ),( Jing Fu Peng ),( Xiao Li Yu ),( Shu Lin Zhang ),( Boping Zhou ),( Dan Qing Jiang ),( Jian Bo Chen ),( Bing Bing Ding ),( Bin Zhu ),( Yao Li ) 한국미생물 · 생명공학회 2016 Journal of microbiology and biotechnology Vol.26 No.1

The widespread occurrence of drug-resistant Mycobacterium tuberculosis places importance on the detection of TB (tuberculosis) drug susceptibility. Conventional drug susceptibility testing (DST) is a lengthy process. We developed a rapid enzymatic color-reaction-based biochip assay. The process included asymmetric multiplex PCR/templex PCR, biochip hybridization, and an enzymatic color reaction, with specific software for data operating. Templex PCR (tem- PCR) was applied to avoid interference between different primers in conventional multiplex- PCR. We applied this assay to 276 clinical specimens (including 27 sputum, 4 alveolar lavage fluid, 2 pleural effusion, and 243 culture isolate specimens; 40 of the 276 were non-tuberculosis mycobacteria specimens and 236 were M. tuberculosis specimens). The testing process took 4.5 h. A sensitivity of 50 copies per PCR was achieved, while the sensitivity was 500 copies per PCR when tem-PCR was used. Allele sequences could be detected in mixed samples at aproportion of 10%. Detection results showed a concordance rate of 97.46% (230/236) in rifampicin resistance detection (sensitivity 95.40%, specificity 98.66%) and 96.19% (227/236) in isoniazid (sensitivity 93.59%, specificity 97.47%) detection with those of DST assay. Concordance rates of testing results for sputum, alveolar lavage fluid, and pleural effusion specimens were 100%. The assay provides a potential choice for TB diagnosis and treatment.

Common Variations of DNA Repair Genes are Associated with Response to Platinum-based Chemotherapy in NSCLCs

Li, Xian-Dong,Han, Ji-Chang,Zhang, Yi-Jie,Li, Hong-Bing,Wu, Xue-Yan Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.1

Aim: Individual differences in chemosensitivity and clinical outcome of non-small-cell lung cancer (NSCLC) patients may be induced by host inherited factors. We investigated the impact of XPD Arg156Arg, XPD Asp312Asn, XPD Asp711Asp and XPD Lys751Gln gene polymorphisms on the efficacy of platinum-based chemotherapy in NSCLC patients. Methods: A total of 496 were consecutively selected from the Affiliated Hospital of Nantong University between Jan. 2003 and Nov. 2006, and all patients were followed-up until Nov. 2011. The genotyping of XPD Arg156Arg, XPD Asp312Asn, XPD Asp711Asp and XPD Lys751Gln was conducted by duplex polymerase-chain-reaction with the confronting-two-pair primer methods. Results: Individuals with XPD 312 C/T+T/T and XPD 711 C/T+T/T exhibited poor responses to chemotherapy when compared with the wild-type genotype, with adjusted ORs(95% CI) of 0.67(0.38-0.97) and 0.54(0.35-0.96), respectively. Cox regression showed the median PFS and OS of patients of XPD 312 C/T+T/T genotype and XPD 711 C/T+T/T genotype to be significantly lower than those with wild-type homozygous genotype. Conclusion: We found polymorphisms in XPD to be associated with response to platinum-based chemotherapy in NSCLC, and our findings provide information for therapeutic decisions for individualized therapy.

Novel wax-crystal modifier based on b-cyclodextrin: Synthesis, characterization and behavior in a highly waxy oil

Bing Wei,Laiming Lu,Hao Li,Yan Xue 한국공업화학회 2016 Journal of Industrial and Engineering Chemistry Vol.43 No.-

A “star-like” architectural wax-crystal modifier based on b-cyclodextrin (P-CD) was successfullydeveloped through a catalytic esterification. This paper mainly investigated the behavior of thiscompound in a waxy oil. The results indicated that P-CD can significantly reduce the oil yield stress anddecrease the wax appearance temperature (WAT). The wax inhibition ratio of P-CD reached up to 50.9%. Cross-Polarized Microscopy (CPM) observations demonstrated that P-CD was capable of hindering waxgrowth. The density function theory (DFT) study verified thefindings from molecular scale, i.e., paraffinswere prone to incorporate with P-CD due to the formation of an extra hydrogen bonding.

Optimization design research of air flow distribution in vertical radial flow adsorbers

Yao Li,Haiqing Si,Bing Wang,Lu Xue,Xiaojun Wu 한국화학공학회 2018 Korean Journal of Chemical Engineering Vol.35 No.4

Non-uniform flow distribution usually exists in a vertical radial flow adsorber, which significantly decreases the utilization of adsorbents. We adopted numerical simulation methods based on the ANSYS Fluent 15.0 software to study the flow pattern in vertical radial flow adsorber, where programs of user-defined functions (UDF) were set up to interpret component equation, momentum equation and energy equation. To solve the problem of non-uniform air distribution, the relationship between the radial pressure drop across the bed and the ratio of cross-sectional area of the central pipe to that of the annular channel was studied, and optimization design of the distributor inserted in the central channel was given by parametric method at the same time. Through comparative analysis in the given experimental condition, the uniformity reached about 99.1% and the breakthrough time extended from 564 s to 1,175 s under the present optimized design method.

Thermal Field Balance and Efficiency Analysis of Plate Type Electromagnetic Induction Heating

Ping Xue,Hao Sun,Bing Li,Hongmin Wang 보안공학연구지원센터 2016 International Journal of Multimedia and Ubiquitous Vol.11 No.2

Owing to flat plate induction heating can’t guarantee to heat equally, especially the existence of no heated zone, it haven’t been used in these industrial fields like SMD which require high accuracy and easy to control while heating. But some places need its characteristic of contact heating, eco-friendly and high efficiency, for this reason, it is fundamental to research the heating style further as well as the no heating zone. Because induction heating is based on eddy current, firstly, the relationship between eddy current and induction heating is explained and several factors which would influence the eddy current are put forward. Then finite element analysis and calculation of eddy current field is mainly done. At last, the conclusion is obtained by analyzing the results of calculation. Due to the analysis above, after improving the structure of the iron plate, the problem of no heating zone is solved. At the same time, the efficiency evaluation is brought out. It can make the following research more easily.

METRIC THEOREM AND HAUSDORFF DIMENSION ON RECURRENCE RATE OF LAURENT SERIES

Hu, Xue-Hai,Li, Bing,Xu, Jian Korean Mathematical Society 2014 대한수학회보 Vol.51 No.1

We show that the recurrence rates of Laurent series about continued fractions almost surely coincide with their pointwise dimensions of the Haar measure. Moreover, let $E_{{\alpha},{\beta}}$ be the set of points with lower and upper recurrence rates ${\alpha},{\beta}$, ($0{\leq}{\alpha}{\leq}{\beta}{\leq}{\infty}$), we prove that all the sets $E_{{\alpha},{\beta}}$, are of full Hausdorff dimension. Then the recurrence sets $E_{{\alpha},{\beta}}$ have constant multifractal spectra.

Pathological Lesions and Inducible Nitric Oxide Synthase Expressions in the Liver of Mice Experimentally Infected with Clonorchis sinensis

Qing-Li Yang,Ji-Qing Shen,Yan Xue,Xiao-Bing Cheng,Zhi-Hua Jiang,Yi-Chao Yang,Ying-Dan Chen,Xiao-Nong Zhou 대한기생충학열대의학회 2015 The Korean Journal of Parasitology Vol.53 No.6

The nitric oxide (NO) formation and intrinsic nitrosation may be involved in the possible mechanisms of liver fluke-associated carcinogenesis. We still do not know much about the responses of inducible NO synthase (iNOS) induced by Clonorchis sinensis infection. This study was conducted to explore the pathological lesions and iNOS expressions in the liver of mice with different infection intensity levels of C. sinensis. Extensive periductal inflammatory cell infiltration, bile duct hyperplasia, and fibrosis were commonly observed during the infection. The different pathological responses in liver tissues strongly correlated with the infection intensity of C. sinensis. Massive acute spotty necrosis occurred in the liver parenchyma after a severe infection. The iNOS activity in liver tissues increased, and iNOS-expressing cells with morphological differences were observed after a moderate or severe infection. The iNOS-expressing cells in liver tissues had multiple origins.

Vibration Isolation of Extended Ultra-high Acceleration Macro-micro Motion Platform Considering Floating Stator Stage

Lufan Zhang,Xue-li Li,Jiwen Fang,Yunhao Lv,Bing Ma,Jun Wu,Hu Li 한국정밀공학회 2019 International Journal of Precision Engineering and Vol.20 No.8

It is a limitation for rapid development of microelectronics manufacturing industry to hardly overcome the acceleration limitation of macro–micro motion platform. The paper presents an extended ultra-high acceleration macro–micro motion platform to investigate breakthrough of acceleration limitation with driving modes “macro + micro + macro” (MMM). In the proposed platform, the number of the pair of VCMs was defined as n. Under ultra-high acceleration, floating stator stage is suggested to isolate the vibration and obtain superior performance of the platform. Its theoretical analyses including natural frequency analysis, transient response analysis and frequency response analysis are performed to verify vibration isolation of floating stator stage applied in the extended ultra-high acceleration macro–micro motion platform. The change trends and sensitivities of related objective functions incorporating vibration transmissibility, settling time and the maximum stroke of stator’s motion are explored with their related parameters, and their multi-objective optimization designs are carried out to achieve superior performances of different extended platforms. Moreover, one case is performed when n = 1. Its theoretical analyses, change trends and sensitivities are investigated. The superior performance of the platform is obtained to realize vibration isolation by multi-objective optimization. And its experiment of vibration isolation is also testified finally. The results provide a theoretical and technical basis on microelectronics manufacturing equipment upgrading and manufacturing rapid development.