골수이식시 채집된 골수혈에서 자동혈액성분분리기를 이용한 적혈구의 선택적 분리

김병수,오상철,최종성,박진호,서재홍,윤수영,임채승,이창규,신상원 大韓血液學會 1996 大韓血液學會誌 Vol.31 No.1

혈액 종양 질환에서 항과립구항체 골수 스캔을 이용한 중심 골수와 말초 골수 분포의 분석

강도영,이재태,손상균,이규보 경북대학교 병원 2003 경북대학교병원의학연구소논문집 Vol.7 No.1

목적: 혈액 종양 질환을 가진 환자들에서 과립구에 대한 단일클론항체를 이용한 골수 스캔을 시행하여 혈액 종양 질환의 종류에 따라서 말초 골수의 분포를 등급화하고 중심성 골수의 정량적 섭취율을 구하였으며 또한 이들 상호간의 관련성을 조사아혔다. 대상 및 방법: 혈액 종양 환자 중에서 골수 조직검사를 시행하여 진단이 확진되고 Tc-99m antigranulocyte monoclonal mouse antibody BW 250/183를 이용한 골수 스캔을 시행한 환자 50명을 대상으로 하였다. 환자는 45군으로 급성 골수성 백혈병 환자 11명, 급성 임파구성 백혈병 환자 12명, 임파선종 환자 15명, 골수이형성증후군 환자 12명이었다. 골수 스캔의 분석은 말초 골수 분포는 골수확장 소견에 따라 4개의 등급으로 분류하였고, 중심성 골수 기능은 장골과 천골에 관심영역을 그려 섭취율을 계산하였다. 결과: 말초 골수 확장의 정도는 전체 환자 50명 중에서 1등급에 4명, 2등급에 27명, 3등급에 15명, 4등급에 4명 이었다. 말초 골수 확장의 정도는 골수이형성증후군과 임파구성 백혈병에서 3, 4등급이 58%(7/12)로 심하였고 임파종에서는 1, 2등급이 93%(14/15)로 가장 경하였으며 질환별로 특이적인 분포는 보이지 않았다. 중심성 골수의 섭취율은 4가지 질환 중 골수이형성증후군에서 8.5±3.6으로 가장 낮았으나 4군 간에 통게적으로 유의한 차이는 없었다(p?.05). 말초 골수 확장 정도에 따른 중심 골수의 정량화는 4가지 등급간에는 섭취율에 있어서 통계적으로도 유의한 차이가 있었으나(p=0.003), 개개의 환자에서 말초 골수 확정 정도와 중심 골수의 정량값의 상관관계는 거의 없었다(r=0.05). 결론: 혈액 종양 질환에서 골수 스캔은 말초 골수의 분포와 중심 골수의 기능에 있어서 질병 특이적인 소견은 보이지 않았으며, 골수의 변화는 말초 골수와 중심 골수에 서로 상관 관계없이 변화를 초래하므로 한 가지 방법으로 보다는 두 가지 방법으로 평가할 필요가 있다. Purpose: Bone marrow scintigraphy has been used to evaluate the status of bone marrow in various hematologic disorders. We have analyzed the peripheral distribution pattern and central uptake ratio of bone marrow using anti-NCA-95 monoclonal antibody and the their correlation in patients with various hematologic malignancy. Materiais and Methods: Bone marrow immunoscintigraphy was performed using Tc-99m anti-granulocyte monoclonal mouse antibody BW 250/183. Fifty patients were classified into four groups; 11 with acute myelogenous leukemia, 12 with acute lymphocytic leukemia, 15 with lymphoma and 12 with myelodysplastic syndrome. The extension of peripheral bone marrow was categorized into four grades: Ⅰ, Ⅱ, Ⅲ and Ⅳ. The activity of central bone marrow was expressed as sacroiliac uptake ratio. Results: The patient's number was 4 in grade Ⅰ, 27 in grade Ⅱ, 15 in grade Ⅲ and 4 in grade Ⅳ according to extension of peripheral bone marrow. The extension of peripheral bone marrow was marked(58% in grade Ⅲ and Ⅳ) in myelodysplastic syndrome and acute lymphocytic leukemia and mild(93% in grade Ⅰ and Ⅱ) in lymphoma. Sacroiliac uptake ratio was highest(8.5±4.0) in myelodysplastic syndrome and lowest(5.9±3.6) in acute myelogenous leukemia, but not significantly different among four patient groups(p>0.05). Sacroiliac uptake ratio of whole patients was significantly different among four grades(p=0.003), but there was not correlated between grade of peripheral bone marrow and sacroiliac uptake ratio(r=0.05). Conclusion: The pattern of peripheral bone marrow extension and activity of central hemopoietic marrow were not specific to the disease entities. Response of hemopoietic bone marrow may be evaluated on both peripheral and central bone marrow in patients with hematologic malignancy.

Dynamic Contrast Enhanced Magnetic Resonance Imaging of Diffuse Spinal Bone Marrow Infiltration in Patients with Hematological Malignancies

Yunfei Zha,Maojin Li,Jianyong Yang 대한영상의학회 2010 Korean Journal of Radiology Vol.11 No.2

Objective: To investigate the significance of the dynamic contrast enhanced magnetic resonance imaging (DCE-MRI) parameters of diffuse spinal bone marrow infiltration in patients with hematological malignancies. Materials and Methods: Dynamic gadolinium-enhanced MR imaging of the lumbar spine was performed in 26 patients with histologically proven diffuse bone marrow infiltration, including multiple myeloma (n = 6), acute lymphoblastic leukemia (n = 6), acute myeloid leukemia (n = 5), chronic myeloid leukemia (n = 7), and non-Hodgkin lymphoma (n = 2). Twenty subjects whose spinal MRI was normal, made up the control group. Peak enhancement percentage (Emax), enhancement slope (ES), and time to peak (TTP) were determined from a timeintensity curve (TIC) of lumbar vertebral bone marrow. A comparison between baseline and follow-up MR images and its histological correlation were evaluated in 10 patients. The infiltration grade of hematopoietic marrow with plasma cells was evaluated by a histological assessment of bone marrow. Results: Differences in Emax, ES, and TTP values between the control group and the patients with diffuse bone marrow infiltration were significant (t = -11.51, -9.81 and 3.91, respectively, p < 0.01). Emax, ES, and TTP values were significantly different between bone marrow infiltration groups Grade 1 and Grade 2 (Z = -2.72, -2.24 and -2.89 respectively, p < 0.05). Emax, ES and TTP values were not significantly different between bone marrow infiltration groups Grade 2 and Grade 3 (Z = -1.57, -1.82 and -1.58 respectively, p > 0.05). A positive correlation was found between Emax, ES values and the histological grade of bone marrow infiltration (r = 0.86 and 0.84 respectively, p < 0.01). A negative correlation was found between the TTP values and bone marrow infiltration histological grade (r = -0.54, p < 0.01). A decrease in the Emax and ES values was observed with increased TTP values after treatment in all of the 10 patients who responded to treatment (t = -7.92, -4.55, and 5.12, respectively, p < 0.01). Conclusion: DCE-MRI of spine can be a useful tool in detecting diffuse marrow infiltration of hematological malignancies, while its parameters including Emax, ES, and TTP can reflect the malignancies’ histological grade. Objective: To investigate the significance of the dynamic contrast enhanced magnetic resonance imaging (DCE-MRI) parameters of diffuse spinal bone marrow infiltration in patients with hematological malignancies. Materials and Methods: Dynamic gadolinium-enhanced MR imaging of the lumbar spine was performed in 26 patients with histologically proven diffuse bone marrow infiltration, including multiple myeloma (n = 6), acute lymphoblastic leukemia (n = 6), acute myeloid leukemia (n = 5), chronic myeloid leukemia (n = 7), and non-Hodgkin lymphoma (n = 2). Twenty subjects whose spinal MRI was normal, made up the control group. Peak enhancement percentage (Emax), enhancement slope (ES), and time to peak (TTP) were determined from a timeintensity curve (TIC) of lumbar vertebral bone marrow. A comparison between baseline and follow-up MR images and its histological correlation were evaluated in 10 patients. The infiltration grade of hematopoietic marrow with plasma cells was evaluated by a histological assessment of bone marrow. Results: Differences in Emax, ES, and TTP values between the control group and the patients with diffuse bone marrow infiltration were significant (t = -11.51, -9.81 and 3.91, respectively, p < 0.01). Emax, ES, and TTP values were significantly different between bone marrow infiltration groups Grade 1 and Grade 2 (Z = -2.72, -2.24 and -2.89 respectively, p < 0.05). Emax, ES and TTP values were not significantly different between bone marrow infiltration groups Grade 2 and Grade 3 (Z = -1.57, -1.82 and -1.58 respectively, p > 0.05). A positive correlation was found between Emax, ES values and the histological grade of bone marrow infiltration (r = 0.86 and 0.84 respectively, p < 0.01). A negative correlation was found between the TTP values and bone marrow infiltration histological grade (r = -0.54, p < 0.01). A decrease in the Emax and ES values was observed with increased TTP values after treatment in all of the 10 patients who responded to treatment (t = -7.92, -4.55, and 5.12, respectively, p < 0.01). Conclusion: DCE-MRI of spine can be a useful tool in detecting diffuse marrow infiltration of hematological malignancies, while its parameters including Emax, ES, and TTP can reflect the malignancies’ histological grade.

항과립구 항체 골수스캔을 이용한 다발성 골수종 병변의 평가 - 단순골 X-선검사 및 골스캔과의 비교

김동환,이재태,백진호,정진태,현동우,천경아,이영학,손상균,송홍석,이규보 ( Dong Hwan Kim,Jae Tae Lee,Jin Ho Baek,Jin Tae Jung,Dong Woo Hyun,Kyung Ah Chun,Young Hak Lee,Sang Kyun Sohn,Hong Seok Song,Kyu Bo Lee ) 대한핵의학회 1998 핵의학 분자영상 Vol.32 No.4

Purpose: Simple X-ray study and bone scan have limiitations for early diagnosis of bone or bone marrow lesions in multiple myeloma. The purpose of this study was to evaluate the diagnostic usefulness of bone marrow imrnunoscintigraphy using anti-granulocyte monoclonal antibody for the evaluation of bone involvement I:n multiple myeloma. Materials and Methods: In 22 patients (Male: 15, Female: 7) with multiple myeloma, we perforrned whole-body immunoscintigraphy using ' Tc-labelled antigranulocyte antibody (BW 250/183, Scintimum Granulozyt CIS, France) and compared the findings with those of simple bone radiography and Tc-MDP bone scan. Abnonnal findings in bone marrow scintigraphy were, considered to be present in case of expansion of peripheral bone marrow or focal photan defect in axial bones. Results: Marrow expansion was noted in 15 of 22 patients (68%). Focal photon defects were found in 18 patients (82%). While one (33%) of 3 patients with Stage II disease showed focal defects in bone marrow scan, abnormal focal defects were observed in 17 of 19 (90%) patients with Stage III. Among 124 focal abnormal sites which were observed in bone marrow scan, bone scan or simple bone radiography, bone rnarrow scan detected 92 sites (74%), whereas 82 sites (66%) were observed in simple bone radiography(58 sites, 47%) or bone scan(40 sites, 32%). Fifty-one (41%) out of 124 bone lesions were detected by bone marrow scan only, and located mostly in thoracolumbar spine. Conclusion: Bone marrow scan using Tc-labelled antigranulocyte antibody seems to be a more sensitive procedure for the detection of pathologic bone lesions than simple bone X-ray ar bone sean in patients with multiple myeloma. (Korean J Nucl Med 1998;32:354-64)

혈액 종양 질환에서 항과립구항체 골수 스캔을 이용한 중심 골수와 말초 골수 분포의 분석

강도영,이재태,손상균,이규보 대한핵의학회 2002 핵의학 분자영상 Vol.36 No.5

목적: 혈액 종양 질환을 가진 환자들에서 과립구에 대한 단일클론항체를 이용한 골수 수캔을 시행하여 혈액 종양 질환의 종류에 따라서 말초 곬의 분포를 등급화하고 중심성 골수의 정량적 섭취율을 구하였으며 또한 이들 상호간의 관련성을 조사하였다. 대상 및 방법: 혈액 종양 환자 중에서 골수 조직검사를 시행하여 진단이 확진되고 Tc-99m anti-granulocyte monoclonal mouse antibody BW 250/183를 이용한 골수 스캔을 시행한 환자 50명을 대상으로 하였다. 환자는 4군으로서 급성 골수성 백혈병 환자 11명, 급성 임파구성 백혈병 환자 12명, 임파선종 환자 15명, 골수이형성증후군 환자 12명이었다. 골수 스캔의 분석은 말초 골수 분포는 골수 확장 소견에 따라 4개의 등급으로 분류하였고, 중심성 골수 기능은 장골과 천골에 관심영역을 그려 섭취율을 계산하였다. 결과: 말초 골수 확장의 정도는 전체 환자 50명 중에서 1등급에 4명, 2등급에 27명, 3등급에 15명, 4등급에 4명이었다. 말초 골수 확장의 정도는 골수이형성증후군과 임파구성 백혈병에서 3, 4등급이 58% (7/12) 심하였고 임파종에서는 1, 2등급이 93% (14/15)로 가장 경하였으며 질환별로 특이적인 분포는 보이지 않았다. 중심성 골수의 섭취율은 4가지 질환 중 골수이형성증후군에서 8.5±4.0으로 가장 높았고, 급성 임파구성 백혈병에서 5.9±3.6으로 가장 낮았으나 4군 간에 통계적으로 유의한 차이는 없었다(p>0.05). 말초 골수 확장 정도에 따른 중심 골수의 정량화는 4가지 등급간에는 섭취율에 있어서 통계적으로도 유의한 차이가 있었으나(p=0.003), 개개의 환자에서 말초 골수 확장 정도와 중심 골수의 정량값의 상관관계는 거의 없었다(r=0.05). 결론: 혈액 종양 질환에서 골수 스캔은 말초 골수의 분포와 중심 골수의 기능에 있어서 질병 특이적인 소견은 보이지 않았으며, 골수의 변화는 말초 골수와 중심 골수에 서로 상관 관계없이 변화를 초래하므로 한 가지 방법으로 보다는 두 가지 방법으로 평가할 필요가 있다. Purpose: Bone marrow scintigraphy has been used to evaluate the status of bone marrow in various hematologic disorders. We have analyzed the peripheral distribution pattern and central uptake ratio of bone marrow using anti-NCA-95 monoclonal antibody and the their correlation in patients with various hematologic malignancy. Materials and Methods: Bone marrow immunoscintigraphy was perfermed using Tc-99m anti-granulocyte monoclonal mouse antibody BW 250/183. Fifty patients were classified into four groups; 11 with acute myelogenous leukemia. 12 with acute lymphocytic leukemia, 15 with lymphoma and 12 with myelodysplastic syndrome. The activity of central bone marrow was expressed as sacroiliac uptake ratio. Results: The patient's number was 4 in grade 1, 27 in grade II, 15 in grade III and 4 in grade IV according to extension of peripheral bone marrow. The extension of peripheral bone marrow was marked (58% in grade III and IV) in myetodysplastic syndrome and acute lymphocyte leukemia and mild (93% in grade I and II) in lymphoma. Sacroiliac uptake ratio was highest (8.5±4.0) in myelodysplastic syndrome and lowest (5.9±3.6) in acute myelogenous leukemia, but not significantly different among four patient groups (p>0.05). Conclusion: The pattern of peripheral bone marrow extension and activity of central hemopoietic marrow were not specific to the disease entities. Response of hemopoietic bone marrow may be evaluated on both peripheral and central bone marrow in patients with hematologic malignancy. (Korean J Nucl Med 2002;36:298-305)

성견 경골에서 자가장골이식이 임플란트 골유착에 미치는 영향

강남연,김명래,김선종 이화여자대학교 의과대학 2009 EMJ (Ewha medical journal) Vol.32 No.2

Objectives:This study was performed to evaluate the effect of autogenous iliac particulated marrow cancellous bone graft on osseointegration of implants in the dog tibia which has a loose bone marrow. Methods:Three mongrel dogs, weighing about15kg or more, and RBM(resorbable blasted media) AVANAⓡ implants(Osstem Inc., Korea) were used in this study. In the control groups, 2 implants sites were prepared conventionally with drills in the left metaphysic of tibia and 2 implants were placed in each dog under general anesthesia. In the experimental groups, 2 implant sites were prepared with drills. and the particulated marrow cancellous bone, which was obtained from the right ilium,was packed into both prepared sites with osteotomes in the right metaphysic of tib-ia in each dog. Two implants were placed. A total of 12 implants were placed in 3 dogs. The animals were sacrificed at 8 weeks after implant placement. The specimens were taken and embedded into the resin. H & E stain was done. The undecalcified specimens were prepared for histological examination and histomorphometric analysis of bone-implant contact ratios. The Mann-Whitney test was used to compare both groups.Values of p<0.01were considered statistically significant. Results:In the cross-sectional radiographs of the control group no trabecular bone was observed in the marrow space and the implant was surrounded with radiolucent marrow space. In the experimental group Some radioaque trabecular bone was observed in the marrow space of tibia and some of the trabecula was contacted with implant surface. Histologically,in the control group few trabecular bone was observed in marrow space and there was poor osseointegration in the marrow. In the experimental group most marrow space was filled with the trabecular boneand the implant surface was contacted with trabecular bone. In histomorphomeric analysis the bone-implant contact ratio of the experimental group(75.6%) was significantly higher than that of the control group(27.1%,p<0.01). Conclusion:The autogeneous bone graft into the loose bone marrow can be an option to improve the bone density and to get better osseointegration of dental implants.

A magnetic resonance imaging study on changes in rat mandibular bone marrow and pulp tissue after high-dose irradiation

이완,이병도,이강규,고광준 대한영상치의학회 2014 Imaging Science in Dentistry Vol.44 No.1

Purpose: This study was designed to evaluate whether magnetic resonance imaging (MRI) is appropriate for detecting early changes in the mandibular bone marrow and pulp tissue of rats after high-dose irradiation. Materials and Methods: The right mandibles of Sprague-Dawley rats were irradiated with 10 Gy (Group 1, n=5) and 20 Gy (Group 2, n=5). Five non-irradiated animals were used as controls. The MR images of rat mandibles were obtained before irradiation and once a week until week 4 after irradiation. From the MR images, the signal intensity (SI) of the mandibular bone marrow and pulp tissue of the incisor was interpreted. The MR images were compared with the histopathologic findings. Results: The SI of the mandibular bone marrow had decreased on T2-weighted MR images. There was little difference between Groups 1 and 2. The SI of the irradiated groups appeared to be lower than that of the control group. The histopathologic findings showed that the trabecular bone in the irradiated group had increased. The SI of the irradiated pulp tissue had decreased on T2-weighted MR images. However, the SI of the MR images in Group 2 was high in the atrophic pulp of the incisor apex at week 2 after irradiation. Conclusion: These patterns seen on MRI in rat bone marrow and pulp tissue were consistent with histopathologic findings. They may be useful to assess radiogenic sclerotic changes in rat mandibular bone marrow. Purpose: This study was designed to evaluate whether magnetic resonance imaging (MRI) is appropriate for detecting early changes in the mandibular bone marrow and pulp tissue of rats after high-dose irradiation. Materials and Methods: The right mandibles of Sprague-Dawley rats were irradiated with 10 Gy (Group 1, n=5) and 20 Gy (Group 2, n=5). Five non-irradiated animals were used as controls. The MR images of rat mandibles were obtained before irradiation and once a week until week 4 after irradiation. From the MR images, the signal intensity (SI) of the mandibular bone marrow and pulp tissue of the incisor was interpreted. The MR images were compared with the histopathologic findings. Results: The SI of the mandibular bone marrow had decreased on T2-weighted MR images. There was little difference between Groups 1 and 2. The SI of the irradiated groups appeared to be lower than that of the control group. The histopathologic findings showed that the trabecular bone in the irradiated group had increased. The SI of the irradiated pulp tissue had decreased on T2-weighted MR images. However, the SI of the MR images in Group 2 was high in the atrophic pulp of the incisor apex at week 2 after irradiation. Conclusion: These patterns seen on MRI in rat bone marrow and pulp tissue were consistent with histopathologic findings. They may be useful to assess radiogenic sclerotic changes in rat mandibular bone marrow.

임상적으로 ITP로 진단받은 환아에게 골수검사의 필요성 유무

조일영,황치관,류혜영,신정원,박경덕 충남대학교 의과대학 지역사회의학연구소 2001 충남의대잡지 Vol.28 No.1

Objective : Bone marrow examination is controversial in childhood idiopathic thrombocyropenic purpura(ITP). The purpose of this study is whether bone marrow examination is needed or not in pediatirc patients clinically diagnosed ITP. Method : To asses the need for bone marrow examination, we followed up 64 patients with presumed ITP. In this patients, Bone marrow examinations were performed in 37 patients at initial diagnosis of ITP and 27 patients are not preformed bone marrow examination at initial diagnosis of ITP. In all 64 patients, the onsets of the disease were abrupt and congenital anomalies were not combined. Physical examination most often reveals mucocutaneous pethechiae and bruise, but lymph node, liver, and spleen were not enlarged. in addition, the complete blood cell count were normal, except for the thrombocytopenia. Results : 1. When clinical ITP was diagnosed initially, bone marrow examinations were preformed in 37 patients. Among these patients, the results of bone marrow examination were not consistent with ITP in 4 patients. In 4 patients, one patient is diagnosed as transient erythroid hypoplasia, another was diagnosed as decreased megakaryocyte, the others were diagnosed as hypocelluar marrow. During following up 37 patients, none had leukemia, aplastic anemia, and other diagnosis. 2. When clinical ITP is diagnosed initially, bone marrow examinations were not performed in 27 patients. these patients were treated with intravenous globulin(IV-G). 5 patients did not response with IV-G. In these 5 patients, only 2 patients progressed to chronic ITP, but were not diagnosed as other disease. Conclusion : Proper history and physical examination as well as a complete blood cell count are reliable means of diagnosing ITP. Bone marrow examination must be considered carefully to ITP patients who are clinically diagnosed.

저골수 충실성 급성 골수성 백혈병의 치료방침 화학 요법과 골수이식

김영균(Young Kyoon Kim),노진탁(Jin Tark Nho),박은영(Eun Young Park),한치화(Chi Wha Han),박종원(Chong Won Park),김춘추(Choon Choo Kim),김동집(Dong Jip Kim),한경자(Kyung Ja Han),김원일(Won Il Kim) 대한내과학회 1988 대한내과학회지 Vol.35 No.6

N/A There have been a few repots of acute myelogenous leukemia (AML) presenting hypocellular bone marrow. Most physicians are reluctant to give these patients intensive chemotherapy because of the potential risk of serious bone marrow failure. We experienced 10 patients with hypocellular AML (HAML) that could be difined by the criteria of 30% or more atypical blasts and 50% or less cellularity in the bone marrow. There were six men and four women and their ages ranged from 20 to 67 years. Various regimens including low dose ara-C alone (4 cases) and low dose ara-C with modified TAD (1case), with mitoxanthrone (1 case) or with mithramycin (1 case) were applied. Of the remaming three patlents; one received supportive care only, another mithramycin alone and the third allogeneic bone marrow transplantation. As a result, the three cases who received either allogeneic bone marrow transplantation or low dose ara-C with modified TAD or that with mitoxanthrone entered into complete remission. However, only the patient who received allogeneic bone marrow transplantation is still alive. The duration of survival ranged from 2 months to 18 months; the median was 8 months. One of the most common complications during chemotherapy was severe bone marrow suppression and the major cause of death was various severe infections during the pancytopenic period. Though the most favorable therapy for HAML is still controversial, bone marrow transplantation is considered to be the choice of treatment at the present time for young patients with HLA-identical donors. If bone marrow transplantation is not available, a more aggressive form of therapy in addition to low dose ara-C will be preferable.

An Immune-compromised Method for Tooth Transplantation Using Adult Bone Marrow Stromal Cells and Embryonic Tooth Germ

권혁제(Hyuk-Jae Kwon),박지희(Gi-Hee Park),김은정(Eun-Jung Kim),이민정(Min-Jung Lee),정한성(Han-Sung Jung),조성원(Sung-Won Cho) 대한체질인류학회 2011 해부·생물인류학 (Anat Biol Anthropol) Vol.24 No.4

소실된 치아를 대체하기 위해 가장 좋은 치료법은 동일개체의 치아 또는 치배를 이식하는 것이지만, 사람에서는 이러한 치아나 치배를 얻기는 매우 힘들다. 동종 또는 이종 간의 치아 이식은 치주인대에서 발생하는 면역거부반응에 의해 대부분 실패하였다. 이번 연구는 성체의 골수간질세포를 배아의 치배와 결합시키는 방법을 사용함으로써, 치아 주위조직의 세포를 골수간질세포로 대치할 수 있는 지에 대해서 알아보았다. 실험결과로서, 골수간질세포에서 유래한 세포들을 치주인대와 이틀뼈에서 관찰할 수 있었다. 이는 골수간질세포가 치주인대와 이틀뼈의 형성에 참여하였다는 것을 의미한다. 만약 이와 같은 치아를 골수간질세포를 제공한 개체에게 이식한다면, 면역거부반응이 덜 일어날 것이다. 이처럼 성체 골수간질세포와 배아 치배를 재결합시키는 방법은 치아주위조직의 일부를 골수간질세포로 바꿔주기 때문에, 이종치배를 이용하여 만든 치아를 사람의 턱뼈에 이식할 수 있도록 하는 방법 중 하나가 될 수 있을 것이다. Tooth transplantation using autogenic adult teeth or embryonic tooth germs is the one of best treatments for replacement of missing teeth, but there are limitations in the accessibility. Isogenic or xenogenic tooth transplantation has been failed because of the immune rejection response occurring in the periodontal ligament of transplanted tooth. In this study, by utilizing the recombination between mouse embryonic tooth germ and mouse adult bone marrow stromal cells, we tried to replace the periodontal tissues such as periodontal ligament and alveolar bone with adult bone marrow stromal cells. At four weeks after the transplantation of the recombinant into a kidney, adult bone marrow-derived cells cells were observed in the periodontal ligament and alveolar bone. This result indicates that adult bone marrow stromal cells can participate in the formation of periodontal tissues. If these tooth and periodontal tissues are transplanted into host who donates adult bone marrow stromal cells, adult bone marrow-derived cells will be regarded as host cells, and immune rejection response will not occur in these cells. Therefore, it is suggested that recombination between adult bone marrow stromal cells and embryonic tooth germ is a good candidate method using xenogenic tooth germ for replacement of missing teeth in human by replacing cells in periodontal tissues with human adult bone marrow stromal cells.