제24회 학술발표회 논문초록

한국연초학회 한국연초학회 1990 한국연초학회지 Vol.12 No.2

시비방법이 버어리종 잎담배의 농경적 특성에 미치는 영향

조천준,배성국,임해건,김요태 한국연초학회 1997 한국연초학회지 Vol.19 No.1

Recently the method of basal compound fertilizer (N : P : K = 18.2 : 9.8 : 35.0) Placement has been changed from banding to broadcasting in tobacco cultivation. The effects of the compound fertilizer placement, level of fertilizer and additional urea application on the agronomic characteristics, two chemical compositions and physical Properties of burley tobacco(Nicotiana tabacum L. cv. KB 108) were investigated at Chonju Experiment Station, Korea Ginseng and Tobacco Research Institute in 1995-1996. Six treatments consisted of (1) band (method of fertilizer Placement) + In kg/10a(compound fertilizer) + 0(additional urea applied), (2) broadcast+140+0, (3) broadcast+140+25, (4) band+180+0, (5) broadcast+180+0, and (6) broadcast +180+25. The additional urea was applied at hilling. No significant differences were detected between banding and broadcasting method of compound fertilizer Placement in field 9rowth, wield, organoleptic qualify(price Per kilogram), chemical composition and Physical properties of cured leaf, The increased compound fertilizer by 30%(40k/10a) or the additional urea application by 25kg per 10a produced slightly higher yield than the recommended amount of basal compound fertilizer without additional urea application did. It also increased the total alkaloid content of cured leaf. It is recommended that no more basal fertilizer above the recommended amount and no additional urea application are needed in burley tobacco fertilization, even though the method of basal fertilizer placement being chanced from banding to broadcasting. Key words : Nicotiana tabacum, fertilizer placement, additional urea.

Latex-Test에 의한 식물 바이러스의 검정

박은경,김정화,이영근 한국연초학회 1979 한국연초학회지 Vol.1 No.1

低濃度의 바이러스 조건하에서도 반응의 민감도가 높은 바이러스病의 血淸學的 診斷法을 개발하고자 담배모자이크 바이러스(Tobacco mosaic virus), 보리줄무늬 모자이크 바이러스( Barleys-tripe mosaic virus) 및 大豆모자이크 바이러스(Soybean mosaic virus)의 抗血淸으로부터 電氣泳動에 의해 추출된 Immunoglobulin을 Latex(0.8l$\mu$, Difco)粒子에 흡착시켜 梢子毛細管(1 $\times$ 100mm)내에서 各 罹病組織의 汁液과 반응시킨 결과 寒天沈降法이나 微量沈降法보다 민감도가 매우 높았다. 이 방법은 작업과정이 간편하고 빠른 시간내에 다량의 시료를 檢定할 수 있으며 陽性反應과 陰性反應을 쉽게 구별할 수 있었다. A simple test using latex-agglutination was developed to detect serologically tobacco mosaic virus (TMV), soybean mosaic virus (SoyMV), and barley stripe mosaic virus (BSMV) from infected Plants. Latex spheres ( 0.81 $\mu$, Difco) were adsorbed with immuno globulin purified by electrophoresis from crude antiserum against viruses. The antibody- sensitized latex suspension was mixed with sap from virus -infected leaves in a glass capillary tube (inner diam. 1mm $\times$ 100 mm length) The mixture, after agitation, was observed under a stereo microscope at low magnification (X20 - X4O), to examine the reaction between antigen (Virus) and its antibody. Flocculation occurred when the reaction was positive.

담배, 토마토 및 고추에서 분리된 TMV 계통의 생물학적, 물리화학적 및 혈청학적 특성

박은경,이청호,이영기,김영호 한국연초학회 1997 한국연초학회지 Vol.19 No.1

Three strains of W isolated from tobacco, tomato and Pepper plants in Korea were characterized based on biological response, serological relationship, and peptide mapping of the capsid Proteins. The strains designated as TMV-common, TMV-Pepper, and TMV-tomato could be distinguishable by different visual symptoms on 3 varieties of tobacco, one variety of tomato and Pepper for each among 27 plant specieces. Serological relationships were examined by agar gel double diffusion test. Only traceable or weak reaction was observed in the incompatible antigen-antibody combinations. The Pepper strain, however, showed trace in reaction with other two antisera. Peptide maps of the capsid proteins digested by V8 protease or by trypsin were also distinguishable, suggesting differences in composition and/or sequence of the amino acids among the strains.

필터 생분해성 증진을 위한 종자 필터 개발 및 적용 효과

김수호,김민규,황의일,한영림,이창국,여운형,Kim, Soo-Ho,Kim, Min-Kyu,Hwang, Eui-Il,Han, Young-Rim,Lee, Chang-Kuk,Yeo, Woon-Hyung 한국연초학회 2014 한국연초학회지 Vol.36 No.1

Cigarette filters containing Brassica Rapa L. seeds of the dark brown and round shaped were evaluated to determine the effect of seed addition on filter degradability. The seeds with germination capability under the tar/nicotine condition in the preliminary test, were put into the active carbon part of the filter(12mm) during filter rod making by the kit. The $4{\pm}2$ pieces of the seeds were put into the opened fiber bundle of the filter tow. In order to test the germination rate of the seeds, seed filters were placed either in a petri dish or test-pot in a conditioned area ($25^{\circ}C$, 70 % RH). The seed filters were placed outdoors exposed to natural conditions with the periodic water supply. The seeds in the smoked filters showed 90 % germination rate after a month under the open air condition. No significant differences in the sensory evaluation and analysis were obtained when the control sample was compared to the same cigarettes with the seeds.

생명공학에 의한 인(P) 자원의 절감과 재이용

김상범,석영선,Kim, Sang-Beam,Seok, Yeong-Seon 한국연초학회 2013 한국연초학회지 Vol.35 No.1

근적외선 분광분석법을 이용한 판상엽 화학성분 평가

한영림,한정호,이호근,제병권,강광원,이기열,어성제,Han, Young-Rim,Han, Jungho,Lee, Ho-Geon,Jeh, Byong-Kwon,Kang, Kwang-Won,Lee, Ki-Yaul,Eo, Seong-Je 한국연초학회 2013 한국연초학회지 Vol.35 No.1

Near InfraRed Spectroscopy(NIRS) is a quick and accurate analytical method to measure multiple components in tobacco manufacturing process. This study was carried out to develop calibration equation of near infrared spectroscopy for the prediction of the amount of chemical components and hot water solubles(HWS) of reconstituted tobacco leaf. Calibration samples of reconstituted tobacco leaf were collected from every lot produced during one year. The calibration equation was formulated as modified partial least square regression method (MPLS) by analyzing laboratory actual values and mathematically pre-treated spectra. The accuracy of the acquired equation was confirmed with the standard error of prediction(SEP) of chemical components in reconstituted tobacco leaf samples, indicated as coefficient of determination($R^2$) and prediction error of sample unacquainted, followed by the verification of model equation of laboratory actual values and these predicted results. As a result of monitoring, the standard error of prediction(SEP) were 0.25 % for total sugar, 0.03 % for nicotine, 0.03 % for chlorine, 0.16 % for nitrate, and 0.38 % for hot water solubles. The coefficient of determination($R^2$) were 0.98 for total sugar, 0.97 for nicotine, 0.96 for chlorine, 0.98 for nitrate and 0.92 for hot water solubles. Therefore, the NIRS calibration equation can be applicable and reliable for determination of chemical components of reconstituted tobacco leaf, and NIRS analytical method could be used as a rapid and accurate quality control method.

한국의 연초 병학 연구사

이영근,Yi, Young-Keun 한국연초학회 2013 한국연초학회지 Vol.35 No.1

The amount and diversity of tobacco pathology research in Korea depend on the change of tobacco cultivation area. Also, the tobacco pathology research was highly influenced by the scientific development of general plant pathology during the period from 1970's to 1990's. Since 1990, the rapid decrease of the tobacco cultivation area resulted in significant decrease of the research manpower. Thus, I dare to foretell few novel studies on tobacco pathology in near future. This review summarizes a research history of tobacco pathology, at which it was once flourished in Korea.

LC-MS/MS를 이용한 담배 중 Amadori Compounds의 분석

민혜정,김영회,이정민,장기철,Min, Hye-Jeong,Kim, Young-Hoi,Lee, Jeong-Min,Jang, Gi-Chul 한국연초학회 2011 한국연초학회지 Vol.33 No.1

Amadori compounds(1-deoxy-1-amino-2-ketoses) are important precursors of color, flavor and aroma produced in foods. Amadori compounds occur naturally in tobacco. The contribution of amadori compounds to smoke quality has been (of) interest because of their roles of the Maillard reaction in the leaf chemistry. The amounts of these compound in tobacco are affected by the processes of aging, drying and storage conditions. In this study, eight compounds were chemically synthesized because amadori compounds (have not been sold commercially these days.) were not available for obtaining commercially. The aim of this study was to develop the analytical method of amadori compounds in tobacco leaf by the liquid chromatography mass spectrometry using triple quadrupole analyzer(LC-MS/MS). This method was simple, rapid, selective and sensitive, and eight amadori compounds were simultaneously and quantitatively analyzed within 20 minutes. This method showed excellent accuracy and precision. Recovery rates of amadori compounds ranged from 86% to 102%, with relative standard deviation(RSD) ranged from 2.6% to 5.9%. This method was applied to analysis of amadori compounds contents of tobacco leaves in different varieties. Furthermore, it was expected that the method could be extended to the analysis of other amadori compounds.

황색종 담배에서 MH, FA, Flumetralin 및 Butralin의 처리방법이 곁순억제에 미치는 영향

류명현,김용암 한국연초학회 2001 한국연초학회지 Vol.23 No.1

Experiments were conducted to evaluated the sucker control efficiency of several suckercides and their combinations for flue-cured tobacco in Korea. In 1999, Flumetralin(Prime+, P+), Butralin(B), Fatty alcohol(FA, n-decanol 85%), pottasium salt of maleic hydrazide(MH), tank mix of MH with P+ (MH·P+), and another tank mix of MH with FA(MH·FA) were applied after topping, respectively, then sucker number and sucker dry weight were investigated on the 30th and 50th day from treatment. Sucker number and dry weight were significantly lowered by P+, B, FA and MH·P+ applications compared with those of MH and MH·FA for both investigated periods. A significant reduction in sucker number and dry weight was observed in P+, B, and MH·P+ applications. FA treatment was not so much effective in sucker control as P+. In 1999 and 2000, several suckercides and tank mixes of their combinations were applied first at button stage and second after topping, respectively, to compare the effects of sucker control on yield, price index and chemical components of cured leaves. Compared to the standard method of FA 30x at button stage followed by MH40x application after topping(FA 30x + MH 40x), sucker number and dry weight decreased by [FA 30x (or P+ 1%] or [FA 30x(or B 1.5%) + MH 60x·B 1.5%] treatments, even at reduced MH levels. But yield, price index and chemical components of cured leaves were not differed among treatments.