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감자 바이러스 Y 비전이성 외피단백질 cDNA의 형질전환에 의한 바이러스 저항성 연초품종 개발
이청호,이영기,강신웅,박성원,김상석,박은경 한국연초학회 1997 한국연초학회지 Vol.19 No.2
Viral coat protein (CP) encoding cDNA with artificial start and stop codons was synthesized by reverse-transcriptase polymerase chain reaction (RT-PCR) from the Korean isolate of potato virus Y-vein nectrosis strain (pVY-VN). To make PVY CP cDNA to untranslatable form, three stop codons were inserted near the start codon by "megaprimer-PCR" method. The untranslatable CP cDNA was subcloned to plant expression vector and transferred to N. tabacum cv. NC82 by Agrobacterium-mediated transformation. Highly resistant plants to PVY infection were screened, based on symptom development after mechanical virus inoculation. By genomic PCR and Southern blot analysis, one or more copies of the untranslatable CP gene were found in all transformants. From northern blot analysis, highly resistant transgenic lines had very low level of CP transcript but susceptible lines had high level, suggesting resistance to PVY infection should be related to RNA-mediated mechanism.mechanism.
이청호,이경호 한일경상학회 2005 韓日經商論集 Vol.31 No.-
This paper aims at analyzing hazard factors to introduce Toyota Production System which is developed by the Toyota Motor Company. Since 1980's global competitiveness of Japanese companies has based on Japanese manufacturing system called the Toyota Production System(TPS) or Lean Production System(LPS). The production system has spread all over the world companies and also has been studied by European or American global companies for a long time. At first, we described the principles of TPS to evaluate the efficiency in production system. Then, we compared Korean firm with Japanese firm with respect to efficient supply of automobile parts to investigate the conditions for a successful adoption of the system. From the survey we found that the labor productivity and product quality of Korean company was inferior to that of Japan's company in spite of the TPS introduction. The result shows that Korea's company do not satisfy the conditions of the TPS. Finally, we proposed important requisitions to effectively introduce the TPS system from Japan. Furthermore, we revealed that well skillful technician and accumulated technology are required to manage the production system effectively. 본 연구는 한국과 일본의 자동차부품기업을 대상으로 토요타생산시스템에 관한 사례연구를 실시하였다. 일본식 생산시스템으로 대표되는 토요타생산시스템은 1980년대 이후 일본 제조업체가 갖고 있는 경쟁력의 토대가 되어 온 생산관리방법이다. 본 연구의 대상기업인 한국의 K사는 자동차부품제조업체로서 일본의 자동차부품 합작기업으로부터 동일한 생산시스템과 기술을 이전받고 있음에도 불구하고 노동생산성과 품질은 일본기업 보다 약 3배 낮은 것으로 나타났다. 이러한 차이의 원인은 한국기업이 토요타생산시스템 도입에 요구되는 조건들을 충족시키지 못한데서 오는 것으로, 본 연구에서는 일본기업과 한국기업에서 토요타생산시스템의 적용상의 차이를 분석함으로써 생산성을 저하시키는 비효율의 원인을 규명하고자 하였다. 분석결과, 지금까지 지적되어 온 한국의 경직된 노사문화 외에도 작업자의 교육훈련과 숙련기능자 형성의 미비, 지속적 개선활동부족 등 생산관리기술상의 문제가 토요타생산시스템 도입을 저해하는 주요 원인인 것으로 조사되었다. 본 연구에서는 일본기업과의 비교분석을 통해 국내기업의 토요타생산시스템의 효율을 높이기 위해 요구되는 노동력 공급, 임금제도, 작업조직 상의 개선점을 제안하였다.
토마토에서 분리된 담배 모자이크 바이러스 외피단백질 유전자의 cDNA 클로닝 및 염기서열 분석
이청호,이영기,강신웅,박은경 한국연초학회 1996 한국연초학회지 Vol.18 No.2
Tobacco mosaic virus (TMV) tomato strain was isolated from tomato "Seo-Kwang" in Korea. The virion was purified by density gradient centrifugation, and total viral RNA was isolated from the purified particles. Coat protein (CP) cDNA of the virus was synthesized by RT-PCR, and the purified cDNA fragment was subcloned to pBluescript II SK-. The analysis of nucleotide sequence showed that this cDNA was 693 nucleotides long from the insert of clone p1571 and p1572 which contain complete codons of the viral coat protein gene (474 nucleotides) and 3' untranslated region. The nucleotides of coat protein encoding cDNA of the strain were 6 nucleotides less than that of TMV common strain isolated from tobacco plant in Korea. The CP gene showed 70% maximum homology with that of the common strain in the nucleotide level and 86% maximum homology in amino acid level.cid level.
From Phenomenology to Ethics: Intentionality and the Other in Marion’s Saturated Phenomenon
이청호 한국윤리학회 2017 倫理硏究 Vol.116 No.1
The “saturated phenomenon” is Jean-Luc Marion’s principal hypothesis, by which he tries to ground the source of phenomenality. Against the transcendental phenomenology, Marion finds phenomena that go beyond the constitutional power of intention. The saturated phenomenon is never possessed because the saturated phenomenon withdraws itself and thus it endlessly escapes from us. A problem of intelligibility thus arises. The essential finitude of the subject requires that the subject passively receives what the saturated phenomenon gives. Marion, however, endows the gifted with more than the mere passivity. The subject is invited as a “witness” who actively responds to the call of the phenomenon. Marion posits the interpersonal relationship. The problem of the interpretability of intention is another problem inherent in the infinity of interpretation of the other. In our ordinary lives, we habitually search out the other’s intention, infinitely. Emmanuel Levinas clearly points out that the other is the transcendent source of ethics, a source which is not intelligible to us. The other, for Levinas, does not appear to the subject, but conditions it. Marion, by contrast, neutralizes the other and “the face” imposes “oneself” as the other who is neutrally visible to us. I assume Marion is more interested in the world of objects, rather than the world of persons, and thus misses the peculiarity resident in the personhood of persons. We become passive in the presence of the personality, not because we want to become passive, but because we realize our own power of illustration does not fill in the personality