Buckling analysis of filament wound composite cylindrical shell for considering the filament undulation and crossover

Zhangxin Guo,Xiaoping Han,Meiqing Guo,Zhijun Han 국제구조공학회 2015 Structural Engineering and Mechanics, An Int'l Jou Vol.55 No.2

The buckling equations of filament wound composite cylindrical shell are established. The coefficients Kij and Lij of the buckling equations are determined by solving the equations. The geometric analysis and the effective stiffness calculation for the fiber crossover and undulation region are respectively accomplished. Using the effective stiffness of the undulation region, the specific formulas of the coefficients Kij and Lij of the buckling equations are determined. Numerical examples of the buckling critical loads have been performed for the different winding angles and stacking sequences cylindrical shell designs. It can be concluded that the fiber undulation results in the less effect on the buckling critical loads Pcr. Pcr increases with the thickness-radius ratio. The effect on Pcr due to the fiber undulation is more obvious with the thickness-radius ratio. Pcr decreases with the length-radius ratio. The effect on Pcr due to the fiber undulation can be neglected when the ratio is large.

Top-emitting organic light-emitting devices based on silicon substrate using Delta -doping technique

Zhijun Wu,Hengqun Guo,Jiaxian Wang 한국물리학회 2011 Current Applied Physics Vol.11 No.2

We have fabricated a Top-emitting organic light-emitting device on silicon substrate Delta -doping technique. Using ultrathin quinacridone as emitting layer, the performance of Top-emitting organic lightemitting device is improved obviously. However, when increasing the thickness of the anode, the performance of device is enhanced dramatically. The max power efficiency of device is 5.9 Lm/W at 5 V corresponding to the current efficiency of 9.3 cd/A. The max current efficiency of device is also increases to 11 cd/A at 7 V.

MicroRNA-217 Functions as a Tumour Suppressor Gene and Correlates with Cell Resistance to Cisplatin in Lung Cancer

Guo, Junhua,Feng, Zhijun,Huang, Zhi'ang,Wang, Hongyan,Lu, Wujie Korean Society for Molecular and Cellular Biology 2014 Molecules and cells Vol.37 No.9

MiR-217 can function as an oncogene or a tumour suppressor gene depending on cell type. However, the function of miR-217 in lung cancer remains unclear to date. This study aims to evaluate the function of miR-217 in lung cancer and investigate its effect on the sensitivity of lung cancer cells to cisplatin. The expression of miR-217 was detected in 100 patients by real-time PCR. The effects of miR-217 overexpression on the proliferation, apoptosis, migration and invasion of SPC-A-1 and A549 cells were investigated. The target gene of miR-217 was predicted by Targetscan online software, screened by dual luciferase reporter gene assay and demonstrated by Western blot. Finally, the effects of miR-217 up-regulation on the sensitivity of A549 cells to cisplatin were determined. The expression of miR-217 was significantly lower in lung cancer tissues than in noncancerous tissues (p < 0.001). The overexpression of miR-217 significantly inhibited the proliferation, migration and invasion as well as promoted the apoptosis of lung cancer cells by targeting KRAS. The up-regulation of miR-217 enhanced the sensitivity of SPC-A-1 and A549 cells to cisplatin. In conclusion, miR-217 suppresses tumour development in lung cancer by targeting KRAS and enhances cell sensitivity to cisplatin. Our results encourage researchers to use cisplatin in combination with miR-217 to treat lung cancer. This regime might lead to low-dose cisplatin application and cisplatin side-effect reduction.

MicroRNA-217 Functions as a Tumour Suppressor Gene and Correlates with Cell Resistance to Cisplatin in Lung Cancer

Junhua Guo,Zhijun Feng,Zhi’ang Huang,Hongyan Wang,Wujie Lu 한국분자세포생물학회 2014 Molecules and cells Vol.37 No.9

MiR-217 can function as an oncogene or a tumour suppressor gene depending on cell type. However, the function of miR-217 in lung cancer remains unclear to date. This study aims to evaluate the function of miR-217 in lung cancer and investigate its effect on the sensitivity of lung cancer cells to cisplatin. The expression of miR-217 was detected in 100 patients by real-time PCR. The effects of miR-217 overexpression on the proliferation, apoptosis, migration and invasion of SPC-A-1 and A549 cells were investigated. The target gene of miR-217 was predicted by Targetscan online software, screened by dual luciferase reporter gene assay and demonstrated by Western blot. Finally, the effects of miR-217 up-regulation on the sensitivity of A549 cells to cisplatin were determined. The expression of miR-217 was significantly lower in lung cancer tissues than in noncancerous tissues (p < 0.001). The overexpression of miR-217 significantly inhibited the proliferation, migration and invasion as well as promoted the apoptosis of lung cancer cells by targeting KRAS. The up-regulation of miR-217 enhanced the sensitivity of SPC-A-1 and A549 cells to cisplatin. In conclusion, miR-217 suppresses tumour development in lung cancer by targeting KRAS and enhances cell sensitivity to cisplatin. Our results encourage researchers to use cisplatin in combination with miR-217 to treat lung cancer. This regime might lead to low-dose cisplatin application and cisplatin side-effect reduction.

Single nucleotide polymorphism-based analysis of the genetic structure of Liangshan pig population

Liu, Bin,Shen, Linyuan,Guo, Zhixian,Gan, Mailing,Chen, Ying,Yang, Runling,Niu, Lili,Jiang, Dongmei,Zhong, Zhijun,Li, Xuewei,Zhang, Shunhua,Zhu, Li Asian Australasian Association of Animal Productio 2021 Animal Bioscience Vol.34 No.7

Objective: To conserve and utilize the genetic resources of a traditional Chinese indigenous pig breed, Liangshan pig, we assessed the genetic diversity, genetic structure, and genetic distance in this study. Methods: We used 50K single nucleotide polymorphism (SNP) chip for SNP detection of 139 individuals in the Liangshan Pig Conservation Farm. Results: The genetically closed conserved population consisted of five overlapping generations, and the total effective content of the population (Ne) was 15. The whole population was divided into five boar families and one non-boar family. Among them, the effective size of each generation subpopulation continuously decreased. However, the proportion of polymorphic markers (P_N) first decreased and then increased. The average genetic distance of these 139 Liangshan pigs was 0.2823±0.0259, and the average genetic distance of the 14 boars was 0.2723±0.0384. Thus, it can be deduced that the genetic distance changed from generation to generation. In the conserved population, 983 runs of homozygosity (ROH) were detected, and the majority of ROH (80%) were within 100 Mb. The inbreeding coefficient calculated based on ROH showed an average value of 0.026 for the whole population. In addition, the inbreeding coefficient of each generation subpopulation initially increased and then decreased. In the pedigree of the whole conserved population, the error rate of paternal information was more than 11.35% while the maternal information was more than 2.13%. Conclusion: This molecular study of the population genetic structure of Liangshan pig showed loss of genetic diversity during the closed cross-generation reproduction process. It is necessary to improve the mating plan or introduce new outside blood to ensure long-term preservation of Liangshan pig.

Adjustment of Hydrophilic-Lipophilic Balance of Hexamethyldisilazane-Modified Nanosilica for Enhanced Oil Recovery

Xiaohe Tao,Sai Guo,Peisong Liu,Xiaohong Li,Zhijun Zhang 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2019 NANO Vol.14 No.2

Different dosages of hexamethyldisilazane (denoted as HMDS), a silane coupling agent, were adopted to modify nanosilica (denoted as NS) to afford a series of HMDS-NS nanoparticles with different hydrophilic-lipophilic balance governed by the amount of surface hydroxyl. The amounts of the hydrophilic hydroxyl of the as-prepared HMDS-NS nanoparticles and their water contact angles were measured, and their dispersing behavior in water and oil was examined in relation to their transfer behavior therein. Moreover, the effects of the as-prepared HMDS-NS nanofluids on the oil–water interfacial tension as well as the oil recovery were investigated based on interfacial tension measurements and simulated rock core flooding tests. Findings indicate that the hydrophilic-lipophilic balance of HMDS-NS nanoparticles highly depends on the amount of the surface hydroxyl, and the surface hydroxyl amount can be well adjusted by properly selecting the dosage of HMDS modifier. Besides, the transfer behavior of HMDS-NS nanoparticles in oil and water is closely related to their hydrophilic-lipophilic balance, and they can greatly reduce the oil–water interfacial tension and increase the oil recovery by 7.7–11.1% as compared with conventional water flooding. This is because the surface grafting of the hydrophobic segments of HMDS leads to a significant increase in the hydrophobicity of nanosilica, thereby changing the wettability of oil on the sand surface and favoring the stripping of oil droplets. Particularly, the HMDS-NS nanofluid obtained with 2wt.% of HMDS modifier has a water contact angle of 83.6 ° and can dramatically reduce the oil–water interfacial tension from 20.22mN/m to 0.28mN/m, showing desired hydrophilic-lipophilic balance and potential for enhanced oil recovery associated with chemical flooding.

Responses to Starch Infusion on Milk Synthesis in Low Yield Lactating Dairy Cows

Zou, Yang,Yang, Zhanshan,Guo, Yongqing,Li, Shengli,Cao, Zhijun Asian Australasian Association of Animal Productio 2015 Animal Bioscience Vol.28 No.9

The effect of starch infusion on production, metabolic parameters and relative mRNA abundance was investigated in low yield lactating cows from 86 days in milk. Six Holstein cows fitted with permanent ruminal cannulas were arranged into one of two complete $3{\times}3$ Latin squares and infused with a starch solution containing 800 grams starch for 16 days. The three treatments were: i) ruminal and abomasal infusion with water (Control); ii) ruminal infusion with cornstarch solution and abomasal infusion with water (Rumen); iii) ruminal infusion with water and abomasal infusion with cornstarch solution (Abomasum). There were no significant differences (p>0.05) among the three treatments with low yield lactating cows in feed and energy intake, milk yield and composition, plasma metabolism, or even on gene expression. However, cows receiving starch through rumen performed better than directly through the abomasum during the glucose tolerance test procedure with a higher area under the curve (AUC; p = 0.08) and shorter half-time ($t^{1/2}$; p = 0.11) of plasma insulin, therefore, it increased glucose disposal, which stated a lipid anabolism other than mobilization after energy supplementation. In conclusion, extra starch infusion at concentration of 800 g/d did not enhance energy supplies to the mammary gland and improve the lactating performance in low yield lactating cows.

Chlorogenic acid accumulation and related gene expression in peach fruit

Yan Juan,Su Ziwen,Guo Shaolei,Zhang Minghao,Zhang Binbin,Cai Zhixiang,Shen Zhijun,Ma Ruijuan,Yu Mingliang 한국원예학회 2022 Horticulture, Environment, and Biotechnology Vol.63 No.3

To reveal the molecular mechanism in the accumulation of chlorogenic acids (CGAs) in peach (Prunus persica) fruit during growth and development, CGA contents in the flesh of the three peach cultivars ‘Ruiguang 18’, ‘Heiyoutao’, and ‘Beijingyixianhong’ were determined. The expression levels of CGA metabolism-related genes were analyzed based on transcriptome data (RNA-seq). These candidate genes were then screened and real-time fluorescent quantitative PCR (qRT-PCR) was performed to verify their expression. The results showed that the content of total CGAs, 5-O-caffeoylquinic acid and 3-O-caffeoylquinic acid, in the flesh of ‘Ruiguang 18’ exhibited a decreasing trend during fruit development, and there was a great drop at maturity stage (P < 0.05). The three contents in ‘Heiyoutao’ increased first at stage S2 (P < 0.05) and then decreased significantly (P < 0.05). In ‘Beijingyixianhong’, they stayed stable in the early stages, then total CGAs and 3-O-caffeoylquinic acid decreased significantly at the maturity stage (P < 0.05). RNA-seq transcriptome data analysis and qRT-PCR expression analysis showed that the accumulation of CGAs in fruit flesh was mainly affected by the expression of Prupe.3G100800 (PpHCT) and Prupe.3G107300 (Pp4CL), and their expression levels were highly consistent with total CGA content. Thus, we concluded that Prupe.3G100800 (PpHCT) and Prupe.3G107300 (Pp4CL) are the key genes for CGAs synthesis in peach flesh.

Effects of β Air Cooling and Subsequent Cold Rolling on Microstructure and Hardness of Zr702 Sheet

Haotian Guan,Lingguo Zeng,Zhijun Li,Linjiang Chai,Yufan Zhu,Yueyuan Wang,Qin Huang,Ke Chen,Liang‑yu Chen,Ning Guo 대한금속·재료학회 2021 METALS AND MATERIALS International Vol.27 No.2

In this work, a Zr702 sheet was subjected to β air cooling and then rolled to 15% reduction at room temperature, with theirdetailed microstructural characteristics characterized by electron channeling contrast imaging and electron backscatterdiffraction techniques. Results show that after the β air cooling, the prior equiaxed grains in the as-received material arecompletely transformed into Widmanstätten structures featured by coarse plates with typical phase transformation misorientationsbetween them. The subsequent 15% rolling allows both slip and twinning (especially the {10–12} type) to beactivated readily, leading to significant grain refinement and the appearance of misorientation angle peaks around 3°–5° and85°. Analyses on kernel average misorientations reveal that there exist very low residual strains in the β-air-cooled specimenwhile they are markedly increased after the 15% rolling and preferably distributed near low-angle and twin boundaries. Hardnessmeasurements show that the specimen hardness is evidently decreased from ~ 199 to ~ 170 HV after the β air cooling,which can be attributed to grain coarsening and the scattered orientations associated with the slow β → α transformation. For the 15%-rolled specimen, however, effective grain refinement by twinning and denser low-angle boundaries jointly leadto ~ 35% hardness increment to ~ 228 HV.

Substituting oat hay or maize silage for portion of alfalfa hay affects growth performance, ruminal fermentation, and nutrient digestibility of weaned calves

Yang Zou,XinPing Zou,XiZhi Li,Gang Guo,Peng Ji,Yan Wang,ShengLi Li,YaJing Wang,ZhiJun Cao 아세아·태평양축산학회 2018 Animal Bioscience Vol.31 No.3

Objective: The impact of forage feeding strategy on growth performance, ruminal fermentation and nutrient digestibility in post-weaning calves was investigated. Methods: Forty-five female Holstein calves (body weight [BW] = 79.79±0.38 kg) were enrolled in the 35-d study at one week after weaning and randomly assigned to one of three dietary treatments. All diets were fed as total mixed ration containing 60% (dry matter [DM] basis) of basal starter feed and 40% (DM basis) of forage, but varied in composition of forage source including i) alfalfa (40% DM, AH); ii) alfalfa hay (26.7% DM)+oat hay (13.3% DM; OH); iii) alfalfa hay (26.7% DM)+corn silage (13.3% DM; WS). Results: Dry matter intake was not different among treatment groups (p>0.05). However, BW (p<0.05) and average daily gain (p<0.05) of calves fed AH and OH were greater than WS-fed calves, whereas heart girth was greater in OH-fed calves than those fed AH and WS (p<0.05). Ruminal fermentation parameters including proportion of butyric acid, acetated-to-propionate ratio, concentration of total volatile fatty acid, protozoal protein, bacterial protein, and microbial protein in rumen were the highest in OH (p<0.05) and the lowest in WS. Compared with the AH and WS, feeding oat hay to postweaning calves increased crude protein digestibility (p<0.05), and decreased duration of diarrhea (p<0.05) and fecal index (p<0.05). Conclusion: Our results suggested that partially replacing alfalfa hay with oat hay improved ruminal fermentation, nitrogen utilization, and reduced incidence of diarrhea in post-weaning dairy calves.