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        Substituting oat hay or maize silage for portion of alfalfa hay affects growth performance, ruminal fermentation, and nutrient digestibility of weaned calves

        Yang Zou,XinPing Zou,XiZhi Li,Gang Guo,Peng Ji,Yan Wang,ShengLi Li,YaJing Wang,ZhiJun Cao 아세아·태평양축산학회 2018 Animal Bioscience Vol.31 No.3

        Objective: The impact of forage feeding strategy on growth performance, ruminal fermentation and nutrient digestibility in post-weaning calves was investigated. Methods: Forty-five female Holstein calves (body weight [BW] = 79.79±0.38 kg) were enrolled in the 35-d study at one week after weaning and randomly assigned to one of three dietary treatments. All diets were fed as total mixed ration containing 60% (dry matter [DM] basis) of basal starter feed and 40% (DM basis) of forage, but varied in composition of forage source including i) alfalfa (40% DM, AH); ii) alfalfa hay (26.7% DM)+oat hay (13.3% DM; OH); iii) alfalfa hay (26.7% DM)+corn silage (13.3% DM; WS). Results: Dry matter intake was not different among treatment groups (p>0.05). However, BW (p<0.05) and average daily gain (p<0.05) of calves fed AH and OH were greater than WS-fed calves, whereas heart girth was greater in OH-fed calves than those fed AH and WS (p<0.05). Ruminal fermentation parameters including proportion of butyric acid, acetated-to-propionate ratio, concentration of total volatile fatty acid, protozoal protein, bacterial protein, and microbial protein in rumen were the highest in OH (p<0.05) and the lowest in WS. Compared with the AH and WS, feeding oat hay to postweaning calves increased crude protein digestibility (p<0.05), and decreased duration of diarrhea (p<0.05) and fecal index (p<0.05). Conclusion: Our results suggested that partially replacing alfalfa hay with oat hay improved ruminal fermentation, nitrogen utilization, and reduced incidence of diarrhea in post-weaning dairy calves.

      • Experimental seismic behavior of RC special-shaped column to steel beam connections with steel jacket

        Jiashu Hao,Qingying Ren,Xingqian Li,Xizhi Zhang,Yongjun Ding,Shaohua Zhang 국제구조공학회 2022 Steel and Composite Structures, An International J Vol.45 No.1

        The seismic performance of the reinforced concrete (RC) special-shaped column to steel beam connections with steel jacket used in the RC column to steel beam fabricated frame structures was investigated in this study. The three full-scale specimens were subjected to cyclic loading. The failure mode, ultimate bearing capacity, shear strength capacity, stiffness degradation, energy dissipation capacity, and strain distribution of the specimens were studied by varying the steel jacket thickness parameters. Test results indicate that the RC special-shaped column to steel beam connection with steel jacket is reliable and has excellent seismic performance. The hysteresis curve is full and has excellent energy dissipation capacity. The thickness of the steel jacket is an important parameter affecting the seismic performance of the proposed connections, and the shear strength capacity, ductility, and initial stiffness of the specimens improve with the increase in the thickness of the steel jacket. The calculation formula for the shear strength capacity of RC special-shaped column to steel beam connections with steel jacket is proposed on the basis of the experimental results and numerical simulation analysis. The theoretical values of the formula are in good agreement with the experimental values.

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        Folic acid supplementation regulates key immunity-associated genes and pathways during the periparturient period in dairy cows

        Khan Muhammad Zahoor,Zhang Zhichao,Liu Lei,Wang Di,Mi Siyuan,Liu Xueqin,Liu Gang,Guo Gang,Li Xizhi,Wang Yachun,Yu Ying 아세아·태평양축산학회 2020 Animal Bioscience Vol.33 No.9

        Objective: The current research was aimed to profile the transcriptomic picture of the peripheral blood lymphocytes (PBLs) associated with immunity in Chinese Holsteins supplemented orally with coated folic acid during the periparturient period. Methods: The total of 123 perinatal cows were selected for this study and divided into three groups; group A (n = 41, 240 mg/500 kg cow/d), group B (n = 40, 120 mg/500 kg cow/d) and group C (n = 42, 0 mg/cow/d) based on the quantity of folic acid fed. Three samples of PBLs were selected from each folic acid treated group (high, low, and control) and RNA sequencing method was carried out for transcriptomic analysis. Results: The analysis revealed that a higher number of genes and pathways were regulated in response to high and low folic acid supplementation compared to the controls. We reported the novel pathways tumor necrosis factor (TNF) signaling, antigen processing and presentation, Staphylococcus aureus infection and nuclear factor (NF)-kappa B signaling pathways) and the key genes (e.g. C-X-C motif chemokine ligand 10, TNF receptor superfamily member 1A, cluster difference 4, major histocompatibility complex, class II, DQ beta, NF-kappa-B inhibitor alpha, and TNF superfamily 13) having great importance in immunity and anti-inflammation in the periparturient cows in response to coated folic acid treatment. Conclusion: Collectively, our study profiled first-time transcriptomic analysis of bovine lymphocytes and compared the involved cytokines, genes, and pathways between high vs control and low vs control. Our data suggest that the low folic acid supplementation (120 mg/500 kg) could be a good choice to boost appropriate immunity and anti-inflammation as well as might being applied to the health improvement of perinatal dairy cows.

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