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      • <i>OsASR5</i> enhances drought tolerance through a stomatal closure pathway associated with ABA and H <sub>2</sub> O <sub>2</sub> signalling in rice

        Li, Jinjie,Li, Yang,Yin, Zhigang,Jiang, Jihong,Zhang, Minghui,Guo, Xiao,Ye, Zhujia,Zhao, Yan,Xiong, Haiyan,Zhang, Zhanying,Shao, Yujie,Jiang, Conghui,Zhang, Hongliang,An, Gynheung,Paek, Nam‐,Cho John Wiley and Sons Inc. 2017 Plant biotechnology journal Vol.15 No.2

        <P><B>Summary</B></P><P>Drought is one of the major abiotic stresses that directly implicate plant growth and crop productivity. Although many genes in response to drought stress have been identified, genetic improvement to drought resistance especially in food crops is showing relatively slow progress worldwide. Here, we reported the isolation of <I>abscisic acid</I>,<I> stress</I> and <I>ripening</I> (<I>ASR</I>) genes from upland rice variety, IRAT109 (<I>Oryza sativa</I> L. ssp. <I>japonica</I>), and demonstrated that overexpression of <I>OsASR5</I> enhanced osmotic tolerance in <I>Escherichia coli</I> and drought tolerance in <I>Arabidopsis</I> and rice by regulating leaf water status under drought stress conditions. Moreover, overexpression of <I>OsASR5</I> in rice increased endogenous ABA level and showed hypersensitive to exogenous ABA treatment at both germination and postgermination stages. The production of H<SUB>2</SUB>O<SUB>2</SUB>, a second messenger for the induction of stomatal closure in response to ABA, was activated in overexpression plants under drought stress conditions, consequently, increased stomatal closure and decreased stomatal conductance. In contrast, the loss‐of‐function mutant, <I>osasr5</I>, showed sensitivity to drought stress with lower relative water content under drought stress conditions. Further studies demonstrated that OsASR5 functioned as chaperone‐like protein and interacted with stress‐related HSP40 and 2OG‐Fe (II) oxygenase domain containing proteins in yeast and plants. Taken together, we suggest that <I>OsASR5</I> plays multiple roles in response to drought stress by regulating ABA biosynthesis, promoting stomatal closure, as well as acting as chaperone‐like protein that possibly prevents drought stress‐related proteins from inactivation.</P>

      • KCI등재

        Identifying natural genotypes of grain number per panicle in rice (Oryza sativa L.) by association mapping

        Jianyin Xie,Fengmei Li,Najeeb Ullah Khan,Xiaoyang Zhu,Xueqiang Wang,Zhifang Zhang,Xiaoqian Ma,Yan Zhao,Quan Zhang,Shuyang Zhang,Zhanying Zhang,Jinjie Li,Zichao Li,Hongliang Zhang 한국유전학회 2019 Genes & Genomics Vol.41 No.3

        Introduction As one of the main yield components, grain number per panicle (GNP) played critical role in the rice yield improvement. The identification of natural advantageous variations under different situations will promote the sustainable genetic improvement in rice yield. Objectives This study was designed to identify natural genotypes in a rice mini-core collection, to examine the genotypic effects across the indica and japonica genetic background in different environments, and excavating the superior genotypes that had drove the modern genetic improvement. Methods The association mapping of GNP was carried out using a mini-core collection including 154 indica and 119 japonica accessions in seven different environments. Genotypic effects of each genotype for each QTL were calculated and genotype frequency distortion between the commercial rice cultivars and landraces was screened by χ2-test. Results In total, 74 QTLs containing stable and sensitive QTLs in various environments were detected. Within them, 20 positive and 24 negative genotypes in indica, and 24 positive and 16 negative genotypes in japonica were identified. When checking the accumulation of positive genotypes identified in indica across cultivars in each of the two subspecies, it indicated that increased number of positive genotypes identified in indica results in the substantially increased GNP in both indica and japonica across all of the environments, while this trend was not obvious for the positive genotypes identified in japonica especially in short day environments. Moreover, the positive and negative genotype frequency distortion between the landraces and commercial rice cultivars indicated that both positive selection of positive genotypes and negative selection of negative genotypes had driven the genetic improvement on GNP. Conclusion Our findings suggested that the accumulation of positive genotypes and purifying negative genotypes played equivalently important roles in the improvement of rice yield, but the efficient use for some QTLs or genotypes depends on the comprehensive evaluation of their effect under diverse genetic backgrounds and environments.

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        A study on aeroelastic forces due to vortex-shedding by reduced frequency response function

        Zhang, Xin,Qian, Zhanying,Chen, Zhen,Zeng, Fanna Techno-Press 2009 Wind and Structures, An International Journal (WAS Vol.12 No.1

        The vortex-induced vibration of an ${\sqcap}$-shaped bridge deck sectional model is studied in this paper via the wind tunnel experiment. The vibratory behavior of the model shows that there is a transition of the predominant vibration mode from the vertical to the rotational degree of freedom as the wind speed increases gradually or vice versa as the wind speed decreases gradually. The vertical vibration is, however, much weaker in the latter case than in the former. This is a phenomenon which is difficult to model by existing parametric models for vortex-induced vibrations. In order to characterize the aeroelastic property of the ${\sqcap}$-shaped sectional model, a time domain force identification scheme is proposed to identify the time history of the aeroelastic forces. After the application of the proposed method, the resultant fluid forces are re-sampled in dimensionless time domain so that reduced frequency response function (RFRF) can be obtained to explore the properties of the vortex-induced wind forces in reduced frequency domain. The RFRF model is proven effective to characterize the correlation between the wind forces and bridge deck motions, thus can explain the aeroelastic behavior of the ${\sqcap}$-shaped sectional model.

      • KCI등재

        A study on aeroelastic forces due to vortex-shedding by reduced frequency response function

        Xin Zhang,Zhanying Qian,Zhen Chen,Fanna Zeng 한국풍공학회 2009 Wind and Structures, An International Journal (WAS Vol.12 No.1

        The vortex-induced vibration of an П-shaped bridge deck sectional model is studied in this paper via the wind tunnel experiment. The vibratory behavior of the model shows that there is a transition of the predominant vibration mode from the vertical to the rotational degree of freedom as the wind speed increases gradually or vice versa as the wind speed decreases gradually. The vertical vibration is, however, much weaker in the latter case than in the former. This is a phenomenon which is difficult to model by existing parametric models for vortex-induced vibrations. In order to characterize the aeroelastic property of the П-shaped sectional model, a time domain force identification scheme is proposed to identify the time history of the aeroelastic forces. After the application of the proposed method, the resultant fluid forces are re-sampled in dimensionless time domain so that reduced frequency response function (RFRF) can be obtained to explore the properties of the vortex-induced wind forces in reduced frequency domain. The RFRF model is proven effective to characterize the correlation between the wind forces and bridge deck motions, thus can explain the aeroelastic behavior of the П-shaped sectional model.

      • KCI등재

        Control Growth of Euphorbia pulcherrima Willd. ex Klotzsch ‘Sonora Jingle’ and ‘Sonora White’ Using Ethephon

        Youping Sun,Lois Berg Stack,Donglin Zhang,Zhanying Gu 한국원예학회 2011 Horticulture, Environment, and Biotechnology Vol.52 No.4

        Ethephon was sprayed on Euphorbia pulcherrima Willd. ex Klotzsch (poinsettia) ‘Sonora Jingle’ and ‘Sonora White’ to control their height and produce more compact potted plants. The results showed that Ethephon could effectively control the growth of ‘Sonora Jingle’ and ‘Sonora White’ poinsettia. Height was reduced by was 33.4 ± 0.8% for ‘Sonora Jingle’ and 30.8 ± 1.3% for ‘Sonora White’ poinsettia, when 700 mg・L^(-1) Ethephon was sprayed three times on 29 August, 20September, and 13 October 2005, respectively. Similar to other plant growth retardants, side effects including phytotoxicity and delays to first bract color were also observed on Ethephon treated poinsettia. However, all plants produced were still of a marketable quality.

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