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Heo, Yunwi,Pyo, Min-Jung,Bae, Seong Kyeong,Lee, Hyunkyoung,Kwon, Young Chul,Kim, Je Hein,Kim, Bokyung,Kim, Choul Goo,Kang, Changkeun,Kim, Euikyung Hindawi Publishing Corporation 2015 Evidence-based Complementary and Alternative Medic Vol.2015 No.-
<P>Bee venom (BV) from honey bee (<I>Apis mellifera L</I>.) has been used in oriental medicine and cosmetic ingredients because of its diverse pharmacological activities. In many studies, among BV components, phospholipase A<SUB>2</SUB> (PLA<SUB>2</SUB>) is known as a major player in BV-induced allergic reaction. Therefore, we removed PLA<SUB>2</SUB> from BV using ultrafiltration and then investigated <I>in vitro</I> phototoxicity and <I>in vivo</I> skin sensitization of PLA<SUB>2</SUB>-free BV (PBV) in comparison with regular BV. The 3T3 neutral red uptake phototoxicity assay can be appropriated to identify the phototoxic effect of a test substance upon the exposure of ultraviolet A. Chlorpromazine, a positive control, showed high levels of photoirritation factor and mean photo effect values, while BV and PBV had less of these values. Local lymph node assay is an alternative method to evaluate skin sensitization potential of chemicals. BALB/c mice were treated with <I>p</I>-phenylenediamine (PPD, positive control), BV, or PBV. In all of PPD concentrations, stimulation indexes (SI) as sensitizing potential of chemicals were ≥1.6, determined to be sensitizer, while SI levels of BV and PBV were below 1.6. Thus, based on these findings, we propose that both BV and PBV are nonphototoxic compounds and nonsensitizers.</P>
화학물질의 개별 및 복합 노출이 제브라피쉬의 행동에 미치는 영향
허윤위(Yunwi Heo),박준우(June-woo Park) 환경독성보건학회 2021 한국독성학회 심포지움 및 학술발표회 Vol.2021 No.5
환경에 존재하는 수많은 화학물질은 물질별로 존재하기보다는 두 가지 이상 복합 조합으로 존재하며, 그 조합은 무수히 많을 것으로 예상된다. 최근 화학물질 노출과 신경계 질환 사이에 연관이 있다는 연구 결과들이 보고되고 있으므로 본 연구에서는 실제 환경 중에 존재할 가능성이 있는 다양한 화학물질 조합으로 신경독성의 지표 중 하나인 행동 영향을 빠르게 스크리닝 하고자 하였다. High throughput screenig system을 활용하여 크기와 형태가 일정한 제브라피쉬의 배아를 선별하여 6 hpf (hour post fertilization)부터 비스페놀류 3종, 프탈레이트류 10종, 파라벤류 4종을 개별 또는 복합 노출하였으며 24시간마다 치사와 부화를 관찰하였다. 120 hpf의 제브라피쉬를 이용하여 thigmotaxis(wall-hugging)와 총 이동 거리를 측정하여 행동 영향 분석을 실시하였다. 비스페놀류와 프탈레이트류를 조합하여 노출한 경우 개별 물질 노출과 비교해 행동 영향이 크게 달라졌으며, 비스페놀류와 파라벤류의 복합물은 thigmotaxis 활성이 증가한 조합들이 총 이동 거리는 감소한 것으로 나타났다. 본 연구를 통해 신경독성의 기초 스크리닝에 해당하는 행동 영향을 평가하였으며, 개별 물질 노출 그룹에 비해 복합물 노출 그룹에서 synergistic 양상을 보이는 것을 확인하였다. 추후 스크리닝한 복합물 조합 중 행동 영향이 나타난 물질을 대상으로 신경독성 및 그 신경계 질환과의 연관 관계를 연구하고자 한다.
The Hair Growth-Promoting Effect of <i> Rumex japonicus</i> Houtt. Extract
Lee, Hyunkyoung,Kim, Na-Hyun,Yang, Hyeryeon,Bae, Seong Kyeong,Heo, Yunwi,Choudhary, Indu,Kwon, Young Chul,Byun, Jae Kuk,Yim, Hyeong Jun,Noh, Byung Seung,Heo, Jeong-Doo,Kim, Euikyung,Kang, Changkeun Hindawi Publishing Corporation 2016 Evidence-based Complementary and Alternative Medic Vol.2016 No.-
<P><I>Rumex japonicus </I>Houtt. is traditionally used as a medicinal plant to treat patients suffering from skin disease in Korea. However, the beneficial effect of<I> Rumex japonicus </I>Houtt. on hair growth has not been thoroughly examined. Therefore, the present study aims to investigate the hair growth-promoting effect of<I> Rumex japonicus </I>(RJ) Houtt. root extract using human dermal papilla cells (DPCs), HaCaT cells, and C57BL/6 mice model. RJ induced antiapoptotic and proliferative effects on DPCs and HaCaT cells by increasing Bcl-2/Bax ratio and activating cellular proliferation-related proteins, ERK and Akt. RJ also increased <I>β</I>-catenin via the inhibition of GSK-3<I>β</I>. In C57BL/6 mice model, RJ promoted the anagen induction and maintained its period. Immunohistochemistry analysis demonstrated that RJ upregulated Ki-67 and <I>β</I>-catenin expressions, suggesting that the hair growth effect of RJ may be mediated through the reinforcement of hair cell proliferation. These results provided important insights for the possible mechanism of action of RJ and its potential as therapeutic agent to promote hair growth.</P>
Improved Therapeutic Profiles of PLA₂-Free Bee Venom Prepared by Ultrafiltration Method
Hyunkyoung Lee,Min-Jung Pyo,Seong Kyeong Bae,Yunwi Heo,Choul Goo Kim,Changkeun Kang,Euikyung Kim 한국독성학회 2015 Toxicological Research Vol.31 No.1
Bee venom (BV) has long been used in traditional Eastern and Western medicine for chronic inflammation, pain and skin therapy. Human exposure to BV, however, often causes unwanted adverse effects and is even fatal in some cases. Phospholipase A₂ (PLA₂) of BV is now suspected to play a key role in these adverse effects. We investigated the potential use of PLA₂-free bee venom (PBV) as a replacement for BV in cosmetic products. PBV prepared by molecular weight cut-off ultrafiltration exhibits a superior profile in comparison with regular BV, by inhibiting elastase activity and suppressing the induction of nitric oxide (NO) and metalloproteinase-9 (MMP-9), while retaining the effects of cell proliferation and protection against ultraviolet B (UVB)-induced damage in human dermal fibroblast cells. PBV thus appears to be more promising than BV as a cosmetic ingredient with a reduced potential for adverse reactions in the recipient.
Anti-wrinkle Effect of PLA2-free Bee Venom against UVB-irradiated Human Skin Cells
Hyunkyoung Lee,Seong Kyeong Bae,Min-Jung Pyo,Yunwi Heo,Choul Goo Kim,Changkeun Kang,Euikyung Kim 경상대학교 농업생명과학연구원 2015 농업생명과학연구 Vol.49 No.1
The use of bee venom (Apis mellifera L., BV) occasionally causes side effects such as inflammation and allergic reactions in the recipients. Several case reports also suggested the treatment of BV has some limitations in its clinical uses, due to the occurrence of dermal necrosis and anaphylatic reactions. It is generally understood that bee venom allergy is mainly the result of its allergic component, phospholipase A2 (PLA2). The present study was aimed to generate PLA2-free bee venom (PBV) and evaluate its efficacy as skin care and cosmetic preparation, comparing with original bee venom (BV). Our results showed that both BV and PBV exhibited significant protective effects in UVB-irradiated human keratinocyte (HaCaT) and human dermal fibroblast (HDF) cells and they also induced type I collagen synthesis in UVB-irradiated HDF cells except BV at 3 μg/ml. Furthermore, BV and PBV showed the inhibition of UVB-stimulated matrix metalloproteinase-1 (MMP-1), a major collagen degrading enzyme in skin. However, BV, unlike PBV, exhibited strong cytotoxicities in skin cells (both HaCaT and HDF) at its working concentrations of anti-wrinkle effect. The underlying cell signaling mechanisms of anti-wrinkle effects of BV and PBV were demonstrated by the activation of ERK1/2, and p38. Conclusively, PBV appears to be the bee venom of choice with less cytotoxicity and higher efficacy on UVB-irradiated skin cells in comparison with original bee venom (BV). Therefore, PBV can better be used as a cosmetic ingredient exhibiting excellent anti-wrinkle effect against photoaging than original BV.