A Dual Selection Marker Transformation System Using Agrobacterium tumefaciens for the Industrial Aspergillus oryzae 3.042

( Yunlong Sun ),( Yali Niu ),( Bin He ),( Long Ma ),( Ganghua Li ),( Van-tuan Tran ),( Bin Zeng ),( Zhihong Hu ) 한국미생물 · 생명공학회 2019 Journal of microbiology and biotechnology Vol.29 No.2

Currently, the genetic modification of Aspergillus oryzae is mainly dependent on protoplast-mediated transformation (PMT). In this study, we established a dual selection marker system in an industrial A. oryzae 3.042 strain by using Agrobacterium tumefaciens-mediated transformation (ATMT). We first constructed a uridine/uracil auxotrophic A. oryzae 3.042 strain and a pyrithiamine (PT)-resistance binary vector. Then, we established the ATMT system by using uridine/uracil auxotrophy and PT-resistance genes as selection markers. Finally, a dual selection marker ATMT system was developed. This study demonstrates a useful dual selection marker transformation system for genetic manipulations of A. oryzae 3.042.

Designing Ternary Laves Phase-Based Eutectic Alloys CrFeNbx with Excellent Strength, Toughness, and Thermal Stability

Yunlong Xue,Yuxuan Wang,Haohua Sun,Na Feng,Liang Yuan 대한금속·재료학회 2023 METALS AND MATERIALS International Vol.29 No.2

Three ternary Laves phase-based eutectic alloys CrFeNbx(x = 2.0, 2.35, 2.7) were designed successfully by combining thebinary phase diagrams and thermodynamics calculations. With the increasing Nb content, the solidified microstructuresevolved from the primary (Cr, Fe)2Nb plus the eutectic (Cr, Fe)2Nb/Nbss in the CrFeNb2.0,via the full eutectic (Cr, Fe)2Nb/Nbss in the CrFeNb2.35,and finally to the primary Nbss plus the eutectic (Cr, Fe)2Nb/Nbss in the CrFeNb2.7. The full eutectic(Cr, Fe)2Nb/Nbss of the CrFeNb2.35was found with outstanding thermal stability, and the lamellar eutectic was quitestable even under the heat-treatment of 1273 K/48 h. The compression strength and fracture toughness increased firstly anddecreased subsequently with the increase of Nb content, which reached their maximums of 2.54 GPa and 18.25 MPa·m1/2,respectively, in the CrFeNb2.35. The excellent combination of compression strength and fracture toughness in the CrFeNbx(x = 2.0, 2.35, 2.7) were attributed to the synergistic effects of Laves phase strengthening, interface strengthening, solidsolution strengthening, alloying toughening and Nbss phase toughening mechanisms.

Distribution of pathological types and epidemiological characteristics based on kidney biopsy in Northwest China

Qin Yunlong,Zhao Jin,Wei Xiao-fei,Wang Yuwei,Yu Zixian,Zhang Yumeng,Sun Shiren 대한신장학회 2023 Kidney Research and Clinical Practice Vol.42 No.1

Background: The spectrum of biopsy-confirmed kidney disease varies with regions and periods. We describe the distribution ofpathological types and epidemiological characteristics of kidney diseases in Northwest China due to regional differences in geographicalenvironment, social economy, and dietary habits.Methods: Kidney biopsy cases from 2005 to 2020 in Xijing Hospital were retrospectively analyzed. Pathological characteristics of patientsin different periods were analyzed using the t test or chi-square test. Joinpoint regression was used to analyze trends in pathologicaltypes and disease spectrum.Results: A total of 10,528 eligible patients were included. Primary glomerular disease (PGD) accounted for the majority of the casesand exhibited an obvious downward trend, whereas secondary glomerular disease (SGD) showed an obvious upward trend. AmongPGD, immunoglobulin A nephropathy (IgAN) remained the most common pathological type, and the detection rate of membranousnephropathy (MN) was significantly increased. Among SGD, Henoch-Schönlein purpura nephritis (HSPN) was the most commonpathological type and may present a significant characteristic of Northwest China. Diabetic nephropathy (DN) exhibited the most obviousupward trend in the whole process, whereas the fastest growth since 2012 was in hypertensive nephropathy.Conclusion: The proportion of SGD increased whereas PGD declined. IgAN remained the most common PGD, and HSPN was themost common SGD. MN and DN showed the most obvious upward trend among PGD and SGD, respectively. Changes in the spectrumof kidney disease, especially the constituent ratio of SGD, pose a great challenge to public health.

Characterization and performance test of phenolphthalein poly(aryl ether ketone) fractionated by incremental poor solvent fractionation

Zengxu Qian,Jingwei Jiang,Yunlong Sun,Qiliang Yuan,Zhichao Wang,Zhipeng Wang,Guangyuan Zhou 한국고분자학회 2023 Macromolecular Research Vol.31 No.8

Though phenolphthalein poly(aryl ether ketone) (PEK-C) has gained attention as a special engineering plastic materials by virtue of its good thermal stability and favorable mechanical properties, its applications are limited due to its wide PDI during polymerization, which result in poor mechanical properties of PEK-C. In order to investigate the effect of molecular weight and polydispersity index (PDI) on PEK-C, an incremental poor solvent fractionation method was used to fractionate PEK-C. The process produced a series of grades with decreasing molecular weight and PDI. The LCP (linear cloud point) equation was combined with the grading method to find the optimal PEK-C/DMAc/ Non-solvent additives (NSA) system, which can be used to improve the grading efficiency. The obtained results showed that the incremental poor solvent fractionation method with the PEK-C/DMAc/EA system made the molecular weight distributed in the range of 25 ~ 113 kDa, PDI mostly distributed in the range of 1.1 ~ 1.4, and the narrowest PDI to reach 1.13, which could not be obtained through the condensation reaction. Meanwhile, we can also achieve expanded production. By fractionation, the elastic modulus of PEK-C film could be increased by up to 22.6%, elongation at break by up to 38.3%, the fracture strength by up to 38.2%, and impact strength of the sheest by up to 41.2%, while the polymer still maintained good thermal and UV shielding properties. This unique Polymer/Solution/NSA system can yield an effective design strategy for controlling its molecular weight and PDI and improving its mechanical performance.

Seismic performance of RC columns with encased prefabricated high-strength CFST core

Yong Yang,Dongde Sun,Yicong Xue,Yunlong Yu,Kang An,Yang Chen 국제구조공학회 2021 Steel and Composite Structures, An International J Vol.39 No.6

This paper proposed an innovative RC column with encased prefabricated high-strength concrete filled steel tube core, and four RC columns with encased prefabricated high-strength CFST core and a RC control-column were tested under lateral low cyclic loading. All specimens were evaluated by the cracks developments, failure patterns, hysteretic behavior, skeleton curves, strength and stiffness degradation, ductility and energy dissipation capacity. The effects of stirrup ratio and welding studs of prefabricated CFST core were investigated in details. The experiment results indicated that compared with the RC control-column, the performances of RC columns with encased prefabricated high-strength CFST core, including the hysteretic behavior, strength degradation, ductility and energy dissipation, were significantly improved. Higher stirrup ratio of the RC column with encased prefabricated high-strength CFST core leaded to higher ductility and more satisfactory energy dissipation capacity, stiffness degradation. Studs could effectively combine prefabricated high-strength CFST core and surrounding concrete, which significantly increase the integrity of RC column with encased prefabricated high-strength CFST core. Based on the test results, a numerical model was established to further analyze the cyclic behavior of the test specimens, and the numerical results agreed well with the test results, which showed the feasibility for the further parametric study. Finally, on the basis of the plastic stress theory, a calculation model for seismic bending moment capacity of RC column with encased prefabricated high-strength CFST core was established, and the results obtained form the formulas showed good agreement with the experiments.

Component Prototyping for the China Spallation Neutron Source Project

Jie Wei,Yanwei Chen,Yunlong Chi,Changdong Deng,Haiyi Dong,Shinian Fu,Wei He,Kaixi Huang,Wen Kang,Jian Li,Huafu Ouyang,Huamin Qu,Caitu Shi,Hong Sun,Jingyu Tang,Juzhou Tao,Sheng Wang,Zhongxiong Xu,Xueju 한국물리학회 2009 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.54 No.5

The China Spallation Neutron Source (CSNS) complex consists of an H- linear accelerator, a rapid cycling synchrotron accelerating the beam to 1.6 GeV, a solid tungsten target station and instruments for spallation neutron applications. The facility operates at a 25-Hz repetition rate with an initial design beam power of 120 kW and is upgradeable to 500 kW. The primary challenge is to build a robust and reliable user-friendly facility with upgrade potential at a fraction of the \world standard" cost. Success of the project relies on the results of prototyping research & development (R&D) of key technical systems and components. This paper discusses the prototyping experiences of the past two and a half years. The China Spallation Neutron Source (CSNS) complex consists of an H- linear accelerator, a rapid cycling synchrotron accelerating the beam to 1.6 GeV, a solid tungsten target station and instruments for spallation neutron applications. The facility operates at a 25-Hz repetition rate with an initial design beam power of 120 kW and is upgradeable to 500 kW. The primary challenge is to build a robust and reliable user-friendly facility with upgrade potential at a fraction of the \world standard" cost. Success of the project relies on the results of prototyping research & development (R&D) of key technical systems and components. This paper discusses the prototyping experiences of the past two and a half years.

Bayesian Hybrid State Estimation for Unequal-length Batch Processes with Incomplete Observations

Guoli Ji,Yaozong Wang,Shunyi Zhao,Yunlong Liu,Kangkang Zhang,Bin Yao,Sun Zhou 제어·로봇·시스템학회 2017 International Journal of Control, Automation, and Vol.15 No.6

This paper investigates state estimation problem for batch processes with unequal-length batches as wellas incomplete observations. A Bayesian hybrid state estimation method is proposed based on two dimensional (2D)correlations of states. The states of equal-length segment of time are estimated according to both within-a-batchand batch-to-batch correlations, and the states of unequal-length segment are obtained according to the correlationswithin the batch. In this way, the batch process states can be achieved in both equal-length and unequal-lengthsituations, of which the latter one is a more general case. In order to approximate state distribution of nonlinearsystem and to deal with the problem of incomplete observations, particle filter (PF) is employed. The proposedmethod shows its superiority with a nonlinear system and a gas-phase reaction process. Compared to a typicalexisting method, the proposed method provides better estimation accuracy in the situation of equal-length batches,also it shows less sensitivity to incomplete observations.

Electrospun SrO-Incorporated Nanofiber Membranes for Biomedical Applications

Zhe Chen,Jitao Fan,Yangfan Ding,Yunlong Zhang,Ahmed I. Abd‑Elhamid,Jinglei Wu,Binbin Sun 한국섬유공학회 2023 Fibers and polymers Vol.24 No.11

Bioactive ceramics have gained increasing interest in biomedical applications. Strontium (Sr)-based ceramics show unique bioactivity in stimulating cell proliferation and migration and promoting angiogenesis. Incorporation of bioactive ceramics into nanofibers represents a facile approach to alter the material properties of electrospun nanofiber membranes. Herein, we prepared SrO-incorporated nanofiber membranes by electrospinning and investigated the effects of SrO particles on the physicochemical properties and cytocompatibility of these membranes. Our results indicate that simple one-stream electrospinning could smoothly introduce SrO particles into poly(L-lactic acid) (PLA)/gelatin nanofibers. SrO-incorporated membranes showed significantly improved wettability but became brittle compared with PLA/gelatin nanofiber membranes. Cells differentially responded to SrO-incorporated membranes: endothelial cells showed greater cell area and coverage on SrO-incorporated membranes; in contrast, fibroblasts were inhibited on these membranes. These findings suggest that SrO-incorporated membranes might find opportunities in specific biomedical applications in which pro-angiogenesis and antiadhesion are simultaneously demanded.

Structural safety reliability of concrete buildings of HTR-PM in accidental double-ended break of hot gas ducts

Guo, Quanquan,Wang, Shaoxu,Chen, Shenggang,Sun, Yunlong Korean Nuclear Society 2020 Nuclear Engineering and Technology Vol.52 No.5

Safety analysis of nuclear power plant (NPP) especially in accident conditions is a basic and necessary issue for applications and commercialization of reactors. Many previous researches and development works have been conducted. However, most achievements focused on the safety reliability of primary pressure system vessels. Few literatures studied the structural safety of huge concrete structures surrounding primary pressure system, especially for the fourth generation NPP which allows existing of through cracks. In this paper, structural safety reliability of concrete structures of HTR-PM in accidental double-ended break of hot gas ducts was studied by Exceedance Probability Method. It was calculated by Monte Carlo approaches applying numerical simulations by Abaqus. Damage parameters were proposed and used to define the property of concrete, which can perfectly describe the crack state of concrete structures. Calculation results indicated that functional failure determined by deterministic safety analysis was decided by the crack resistance capability of containment buildings, whereas the bearing capacity of concrete structures possess a high safety margin. The failure probability of concrete structures during an accident of double-ended break of hot gas ducts will be 31.18%. Adding the consideration the contingency occurrence probability of the accident, probability of functional failure is sufficiently low.

Analysis of Laser-protection Performance of Asymmetric-phase-mask Wavefront-coding Imaging Systems

Yangliang Li,Qing Ye,Lei Wang,Hao Zhang,Yunlong Wu,Xian’an Dou,Xiaoquan Sun 한국광학회 2023 Current Optics and Photonics Vol.7 No.1