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Magnetic Resonance Imaging Features of Syringobulbia in Small Breed Dogs
Youngmok Song,In Lee,Yumi Song,Ho-Jung Choi,Youngwon Lee 대한수의학회 2023 大韓獸醫學會誌 Vol.63 No.3
Syringobulbia is a rare neurological disorder characterized by a fluid-filled cavity in the brainstem. In this study, clinical signs, features on magnetic resonance imaging (MRI), and the diseases present concurrently with syringobulbia were investigated in 33 small breed dogs. Most dogs (97%) had concurrent syringomyelia, and some dogs (24%) presented with vestibular or cranial nerve symptoms associated with the medulla oblongata. MRIs revealed slit-like, bulbous, and vertical linear shapes of the cavities on T2-weighted hyperintense and T1-weighted hypointense signals similar to the cerebrospinal fluid. Chiari-like malformations were identified in all dogs. This study highlights the association of syringobulbia with syringomyelia and Chiari-like malformations in small breed dogs with or without brainstem-associated clinical signs.
Shim, Yumi,Nam, Myung Hyun,Hyuk, Song Woo,Yoon, Soo Young,Song, Joon Myong Elsevier 2015 Analytica chimica acta Vol.853 No.-
<P><B>Abstract</B></P> <P>The circulating endothelial progenitor cells (EPCs) in blood of acute myocardial infarction (AMI) patient have been monitored in many previous studies. The number of circulating EPC increases in the blood of patients at onset of the AMI. EPC is originated from bone marrow. It performs vessel regeneration. There are many markers used for detecting EPC. Four of these markers, CD31, CD34, CD45, and CD146, were concurrently detected at the single cell level for the identification of EPC in the present preliminary study. The CD45 negative cell sorting was performed to peripheral blood mononuclear cells (PBMCs) acquired from four AMI patients with a magnetic bead sorter, since, EPCs expressed CD45 negative or dim. The resultant PBMC eluents were treated with quantum-antibody conjugates for the probing four different markers of EPCs and then applied to a high-content single cell imaging cytometer using acousto-optical tunable filter (AOTF). The use of quantum dot, with narrow emission wavelength range and AOTF enabling cellular image at a particular single wavelength, is very advantageous for accurate high-content AMI diagnosis based on simultaneous monitoring of many markers. The number of EPC increased as compared with control in three of four AMI patients. In this approach, two EPC subtypes were found, CD31(+), CD34(+), CD45(−/dim), CD146(−) as early outgrowth EPCs and CD31(+), CD34(+), CD45(−/dim), CD146(+) as late outgrowth EPCs. Patient 1 had CD31(+), CD34(+), CD45(−/dim), CD146(+) cells whose percentage was 4.21% of cells. Patient 2 had 2.38% of CD31(+), CD34(+), CD45(−/dim), CD146(−) cells and patient 3 had 4.28% of CD31(+), CD34(+), CD45(−/dim), CD146(+) cells.</P> <P><B>Highlights</B></P> <P> <UL> <LI> We observe EPCs and HPCs in patient for AMI diagnosis. </LI> <LI> We detect two EPC subtypes using quantum dot and AOTF. </LI> <LI> Quantum dot has narrower emission wavelength range than fluorescence dye. </LI> <LI> AOTF provide smaller spectral interference than bandpass filters. </LI> <LI> Quantum dot and AOTF are suitable for detecting large number of molecular markers concurrently. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>
Song, Juhyun,Park, Joohyun,Oh, Yumi,Lee, Jong Eun Hindawi Publishing Corporation 2015 Oxidative medicine and cellular longevity Vol.2015 No.-
<P>Ischemic stroke interrupts the flow of blood to the brain and subsequently results in cerebral infarction and neuronal cell death, leading to severe pathophysiology. Glutathione (GSH) is an antioxidant with cellular protective functions, including reactive oxygen species (ROS) scavenging in the brain. In addition, GSH is involved in various cellular survival pathways in response to oxidative stress. In the present study, we examined whether GSH reduces cerebral infarct size after middle cerebral artery occlusion <I>in vivo</I> and the signaling mechanisms involved in the promotion of cell survival after GSH treatment under ischemia/reperfusion conditions <I>in vitro</I>. To determine whether GSH reduces the extent of cerebral infarction, cell death after ischemia, and reperfusion injury, we measured infarct size in ischemic brain tissue and the expression of claudin-5 associated with brain infarct formation. We also examined activation of the PI3K/Akt pathway, inactivation of FOXO3, and expression of Bcl2 to assess the role of GSH in promoting cell survival in response to ischemic injury. Based on our results, we suggest that GSH might improve the pathogenesis of ischemic stroke by attenuating cerebral infarction and cell death.</P>
Effects of Aging on Transformation of Biogenic Magnetite Nanoparticles
Kim, Yumi,Song, Yungoo,Roh, Yul American Scientific Publishers 2018 Journal of nanoscience and nanotechnology Vol.18 No.2
<P>This study examined the microbial synthesis of magnetite and Pd/Zn-substituted magnetite using metal-reducing bacteria (Clostridium sp.), and the mineralogical characteristics of various types of magnetite formed initially and transformed minerals aged for 5 years. XRD, SXRD, and TEM-EDS analyses were used to characterize the mineralogy, crystal structure, chemistry, shape, and size distribution of the magnetites and transformed minerals. The metal-reducing bacteria reduced akaganeite and Pd/Zn-akaganeite to magnetite and Pd/Zn-substituted magnetite using glucose as an electron donor, respectively. Metal substitution of Pd and Zn within the magnetite structure resulted in a decrease in the unit-cell parameter of the magnetite crystals. After 5 years, the biogenic magnetite showed changes in unit-cell parameters and transformation to siderite during prolonged cultivation under anaerobic conditions. These results indicate that long-term aging may affect the mineralogical transformation and alter the nano-sized crystal structure.</P>
Shim, Yumi,Song, Joon Myong The Royal Society of Chemistry 2015 Chemical communications Vol.51 No.11
<P>In this study, it was found that breast cancer stem cells (CSCs) are formed from MCF-7 cells by benzo[<I>a</I>]pyrene (BP)-induced mutation. The breast CSCs were detected through simultaneous monitoring of CD44, CD24 and aldehyde dehydrogenase 1 (ALDH1) by hypermulticolor cellular imaging using an acousto-optical tunable filter (AOTF) and quantum dots (Q-dots).</P> <P>Graphic Abstract</P><P>In this study, it was found that breast cancer stem cells (CSCs) are formed from MCF-7 cells by benzo[<I>a</I>]pyrene (BP)-induced mutation. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=c4cc08953g'> </P>
Multi-voxel magnetic resonance spectroscopy of cerebral metabolites in healthy dogs at 1.5 Tesla
Sooyoung Choi,Yumi Song,Kija Lee,이영원,최호정 대한수의학회 2016 Journal of Veterinary Science Vol.17 No.2
This study was conducted to measure the difference in levels of cerebral metabolites in the right and left hemispheres, gray (GM) and white matter (WM), imaging planes, and anatomical regions of healthy dogs to establish normal variations. Eight male Beagle dogs (1 to 4 years of age; mean age, 2 years) with no evidence of neurologic disease were studied. Using the multi-voxel technique on a 1.5 Tesla magnetic resonance imaging scanner, metabolite values (N-acetyl aspartate [NAA], choline [Cho], creatine [Cr]) were obtained from the frontoparietal WM, parietal GM, temporal GM, occipital GM, thalamus, cerebellum, mid-brain, and pons. There was no significant difference in levels of these metabolites between the right and left in any locations or between the GM and WM in the cerebral hemispheres. However, there were significant differences in metabolite ratios within imaging planes. The NAA/Cr was lower in the cerebellum than other regions and the thalamus had a higher Cho/Cr and lower NAA/Cho ratio than in other regions. The spectral and metabolic values will provide a useful internal reference for clinical practice and research involving multi-voxel magnetic resonance spectroscopy. Measurement of metabolite values in the transverse plane is recommended for comparing levels of regional metabolites.
Isolation of a Tryptophan - Overproducing Strain Generated by EMS Mutagenesis of Candida rugosa
Sunhye Kim,Yumi Ha,Hyoungjoon Youm,Gilhwan An,Bongduk Lee,Misun Won,Kyung Bin Song 한국식품영양과학회 2004 Preventive Nutrition and Food Science Vol.9 No.2
To isolate a mutant strain which overproduces tryptophan, mutants of Candida rugosa were screened after EMS (ethyl methane sulfonate) mutagenesis. Fluorotryptophan, a tryptophan analogue, was used for selection of a tryptophan-overproducing mutant after mutagenesis. Among 50 mutants, several candidates were selected based on intracellular tryptophan content. Amino acid analysis results showed that C3 was the best strain because it had the highest amount of tryptophan among the mutants.