IRS-2 Partially Compensates for the Insulin Signal Defects in IRS-1^-/- Mice Mediated by miR-33

Tang, Chen-Yi,Man, Xiao-Fei,Guo, Yue,Tang, Hao-Neng,Tang, Jun,Zhou, Ci-La,Tan, Shu-Wen,Wang, Min,Zhou, Hou-De Korean Society for Molecular and Cellular Biology 2017 Molecules and cells Vol.40 No.2

Insulin signaling is coordinated by insulin receptor substrates (IRSs). Many insulin responses, especially for blood glucose metabolism, are mediated primarily through Irs-1 and Irs-2. Irs-1 knockout mice show growth retardation and insulin signaling defects, which can be compensated by other IRSs in vivo; however, the underlying mechanism is not clear. Here, we presented an Irs-1 truncated mutated mouse ($Irs-1^{-/-}$) with growth retardation and subcutaneous adipocyte atrophy. $Irs-1^{-/-}$ mice exhibited mild insulin resistance, as demonstrated by the insulin tolerance test. Phosphatidylinositol 3-kinase (PI3K) activity and phosphorylated Protein Kinase B (PKB/AKT) expression were elevated in liver, skeletal muscle, and subcutaneous adipocytes in Irs-1 deficiency. In addition, the expression of IRS-2 and its phosphorylated version were clearly elevated in liver and skeletal muscle. With miRNA microarray analysis, we found miR-33 was down-regulated in bone marrow stromal cells (BMSCs) of $Irs-1^{-/-}$ mice, while its target gene Irs-2 was up-regulated in vitro studies. In addition, miR-33 was down-regulated in the presence of Irs-1 and which was up-regulated in fasting status. What's more, miR-33 restored its expression in re-feeding status. Meanwhile, miR-33 levels decreased and Irs-2 levels increased in liver, skeletal muscle, and subcutaneous adipocytes of $Irs-1^{-/-}$ mice. In primary cultured liver cells transfected with an miR-33 inhibitor, the expression of IRS-2, PI3K, and phosphorylated-AKT (p-AKT) increased while the opposite results were observed in the presence of an miR-33 mimic. Therefore, decreased miR-33 levels can up-regulate IRS-2 expression, which appears to compensate for the defects of the insulin signaling pathway in Irs-1 deficient mice.

Roles of GST-π and polβ Genes in Chemoresistance of Esophageal Carcinoma Cells

Tang, Yue,Xuan, Xiao-Yan,Li, Min,Dong, Zi-Ming Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.12

The main aim of this study was to investigate the roles of GST-${\pi}$ and $pol{\beta}$ genes in the chemoresistance of esophageal carcinoma cells. Eukaryotic expression vectors containing each gene were constructed and transfected into EC9706 cells, and the biological effects of the two genes assessed based on a resistance index. We additionally investigated the in vitro and in vivo anti-resistance effects of GST-${\pi}$ and $pol{\beta}$ genes using recombinant lentiviruses carrying siRNAs against the two genes. Our results showed that upregulation of GST-${\pi}$ and $pol{\beta}$ genes suppresses chemosensitivity of esophageal carcinoma cells to cisplatin, while downregulation of these two genes with RNAi technology reverses this chemoresistance. Multi-site injection of recombinant lentivirus targeting the GST-${\pi}$ gene into transplanted cDDP tumors effectively reversed their chemoresistant phenotype. However, the same treatment against the $pol{\beta}$ gene did not lead to significant efficacy against chemoresistance.

Optimization of impulse water turbine based on GA-BP neural network arithmetic

Lingdi Tang,Shouqi Yuan,Yue Tang,Zhipeng Qiu 대한기계학회 2019 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.33 No.1

To develop an optimum design method for impulse water turbines with low specific speed, a representative impulse water turbine with low specific speed used in agricultural irrigation machinery was optimized with a combination of an orthogonal experimental design, a genetic algorithm, and a BP neural network in this study. Numerical calculation was applied to analyze interflow characteristics for optimized and original water turbines. Results showed that the internal flow characteristics of the optimized water turbine presented remarkable improvement compared with the original water turbine. Pressure distribution increased, the vortex strip in the draft tube was reduced remarkably, and impeller torque increased by 26 %. In addition, the optimized impeller was manufactured by 3D printing, and performance comparison was conducted between experiments of the optimized and original water turbines. The efficiency of the optimized water turbine reached 42.5 %, which exceeded the original water turbine’s of 8.5 %. With increasing rotating speed, maximum efficiency running point moved to a high flow rate, and highly efficient areas expanded. Internal characteristic analysis and a full-scale experiment for both water turbines showed that the performance of the optimized water turbine exhibited substantial improvement. The analysis and experiment also verified the theoretical correctness and feasibility of the proposed optimum design method.

IRS-2 Partially Compensates for the Insulin Signal Defects in IRS-1−/−Mice Mediated by miR-33

Chen-Yi Tang,Xiao-Fei Man,Yue Guo,Hao-Neng Tang,Jun Tang,Ci-La Zhou,Shu-Wen Tan,Min Wang,Hou-De Zhou 한국분자세포생물학회 2017 Molecules and cells Vol.40 No.2

Insulin signaling is coordinated by insulin receptor substrates (IRSs). Many insulin responses, especially for blood glucose metabolism, are mediated primarily through Irs-1 and Irs-2. Irs-1 knockout mice show growth retardation and insulin signaling defects, which can be compensated by other IRSs in vivo; however, the underlying mechanism is not clear. Here, we presented an Irs-1 truncated mutated mouse (Irs-1−/−) with growth retardation and subcutaneous adipocyte atrophy. Irs-1−/− mice exhibited mild insulin resistance, as demonstrat-ed by the insulin tolerance test. Phosphatidylino-sitol 3-kinase (PI3K) activity and phosphorylated Protein Kinase B (PKB/AKT) expression were elevated in liver, skeletal muscle, and subcu-taneous adipocytes in Irs-1 deficiency. In addition, the expression of IRS-2 and its phosphorylated version were clearly elevated in liver and skeletal muscle. With miRNA microarray analysis, we found miR-33 was down-regulated in bone marrow stromal cells (BMSCs) of Irs-1−/− mice, while its target gene Irs-2 was up-regulated in vitro studies. In addition, miR-33 was down-regulated in the presence of Irs-1 and which was up-regulated in fasting status. What’s more, miR-33 restored its expression in re-feeding status. Meanwhile, miR-33 levels decreased and Irs-2 levels increased in liver, skeletal muscle, and subcutaneous adipocytes of Irs-1−/− mice. In primary cultured liver cells transfected with an miR-33 inhibitor, the expression of IRS-2, PI3K, and phosphorylated-AKT (p-AKT) increased while the opposite results were observed in the presence of an miR-33 mimic. Therefore, decreased miR-33 levels can up-regulate IRS-2 expression, which appears to compensate for the defects of the insulin signaling pathway in Irs-1 deficient mice.

All-trans-retinoic Acid Promotes Iodine Uptake Via Up-regulating the Sodium Iodide Symporter in Medullary Thyroid Cancer Stem Cells

Tang, Min,Hou, Yan-Li,Kang, Qiang-Qiang,Chen, Xing-Yue,Duan, Li-Qun,Shu, Jin,Li, Shao-Lin,Hu, Xiao-Li,Peng, Zhi-Ping Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.4

Recently, the main therapy of medullary thyroid cancer (MTC) is surgical, but by which way there is a poor prognosis with a mean survival of only 5 years. In some cases, some researchers found that it is the medullary thyroid cancer stem cells (MTCSCs) that cause metastasis and recurrence. This study aimed to eradicate MTCSCs through administration of all-trans-retinoic acid (ATRA). Here we demonstrate that MTCSCs possess stemlike properties in serum-free medium. The ABCG2, OCT4 and sodium iodide symporter (NIS) were changed by ATRA. Additionally, we found that ATRA can increase the expression of NIS in vivo. All the data suggested that ATRA could increase the iodine uptake of MTCSCs through NIS.

Ginsenosides as dietary supplements with immunomodulatory effects: a review

Tang Ping,Liu Sitong,Zhang Junshun,Ai Zhiyi,Hu Yue,Cui Linlin,Zou Hongyang,Li Xia,Wang Yu,Nan Bo,Wang Yuhua 한국응용생명화학회 2024 Applied Biological Chemistry (Appl Biol Chem) Vol.67 No.-

Immune disorders have become one of the public health problems and imposes a serious economic and social burden worldwide. Ginsenosides, the main active constituents of ginseng, are regarded as a novel supplementary strategy for preventing and improving immune disorders and related diseases. This review summarized the recent research progress of ginsenosides in immunomodulation and proposed future directions to promote the development and application of ginsenosides. After critically reviewing the immunomodulatory potential of ginsenosides both in vitro and in vivo and even in clinical data of humans, we provided a perspective that ginsenosides regulated the immune system through activation of immune cells, cytokines, and signaling pathways such as MAPK, PI3K/ Akt, STAT, and AMPK, as well as positively affected immune organs, gut flora structure, and systemic inflammatory responses. However, the evidence for the safety and efficacy of ginsenosides is insufficient, and the immune pathways of ginsenosides remain incompletely characterized. We believe that this review will provide a valuable reference for further research on ginsenosides as dietary supplements with immunomodulatory effects.

A Generalized CNN Model with Automatic Hyperparameter Tuning for Millimeter Wave Channel Prediction

Yue, Chengfang,Tang, Hui,Yang, Jun,Chai, Li 한국통신학회 2023 Journal of communications and networks Vol.25 No.4

This paper focuses on millimeter wave (mmWave)channel prediction by machine learning (ML) methods. PreviousML-based mmWave channel predictors have limitations on re-quirements of the amount of training data, model generalizationability, robustness to noise, etc. In this paper, we propose aCNN model with a novel feature selection strategy for mmWavechannel prediction. Automatic hyperparameter tuning (AHT)algorithms are embedded in the training process to iterativelyoptimize the predictive performance of the proposed CNN. Thediversification strategy is leveraged to enhance the robustness ofthe AHT procedure against different communication scenarios. To improve the generalization ability of the prediction model, theinput features are designed to capture the correlation betweenthe physical environment and the channel characteristics. Inparallel, the Cartesian coordinates of the transmitter (Tx) and re-ceiver (Rx) are transformed into polar ones to reduce the model’ssensitivity to coordinate noise. Numerical results demonstrate theeffectiveness of the proposed CNN model in predicting mmWavechannel characteristics in various communication scenarios.

Liver kinase B1 (LKB1) reduced inflammation and oxidative stress by regulating the AMPK/NLRP3 signaling pathway in LPS-induced lung injury

Yue Yifeng,Zong Liwu,Chen Yongmin,Nianhai Feng,Tang Junxia,Xu Hongyu,Zhao Meiling 대한독성 유전단백체 학회 2021 Molecular & cellular toxicology Vol.17 No.4

Background Inflammation and oxidative stress-induced molecular death are one of the important causes for lung injury in critically ill patients. LKB1 is an important protein kinase in the body and has regulated inflammation and oxidative stress. However, LKB1 control inflammation and oxidative stress in lung injury were unclear. Objective We aimed to investigate the function and mechanism of Liver kinase B1 (LKB1) in lipopolysaccharide (LPS)-induced lung injury. Result LKB1 prevented lung injury, and weakened correlation oxidative stress and inflammatory reaction in LPS-induced mice model of lung injury. Up-regulation of LKB1 reduced reactive oxygen species (ROS) production and it-induced oxidative stress, and weakened inflammatory reactions in LPS-induced lung injury A549 cells. Down-regulation of LKB1 increased ROS production and it-induced oxidative stress, and enhanced inflammatory reactions in LPS-induced lung injury A549 cells. LKB1 induced phosphorylation (p)-AMPK protein expression, and suppressed the protein expression of NLRP3 in LPS-induced mice model of lung injury and in LPS-induced lung injury A549 cells. This experiment demonstrated the inhibition of AMPK or activation of NLRP3 inflammasome reversed the anti-inflammation function of LKB1 in LPS-induced lung injury. Meanwhile, ROS-induced oxidative stress also participated in the anti-inflammation effects of LKB1 in LPS-induced lung injury. Conclusion Therefore, our results indicate that LKB1 reduced inflammation and oxidative stress by regulating the AMPK/NLRP3 signaling pathway in LPS-induced lung injury.

Biomimetic Biphasic Electrospun Scaffold for Anterior Cruciate Ligament Tissue Engineering

Tang Ya,Tian Jialiang,Li Long,Huang Lin,Shen Quan,Guo Shanzhu,Jiang Yue 한국조직공학과 재생의학회 2021 조직공학과 재생의학 Vol.18 No.5

Background: Replacing damaged anterior cruciate ligaments (ACLs) with tissue-engineered artificial ligaments is challenging because ligament scaffolds must have a multiregional structure that can guide stem cell differentiation. Here, we designed a biphasic scaffold and evaluated its effect on human marrow mesenchymal stem cells (MSCs) under dynamic culture conditions as well as rat ACL reconstruction model in vivo. Methods: We designed a novel dual-phase electrospinning strategy wherein the scaffolds comprised randomly arranged phases at the two ends and an aligned phase in the middle. The morphological, mechanical properties and scaffold degradation were investigated. MSCs proliferation, adhesion, morphology and fibroblast markers were evaluated under dynamic culturing. This scaffold were tested if they could induce ligament formation using a rodent model in vivo. Results: Compared with other materials, poly(D,L-lactide-co-glycolide)/poly(ε-caprolactone) (PLGA/PCL) with mass ratio of 1:5 showed appropriate mechanical properties and biodegradability that matched ACLs. After 28 days of dynamic culturing, MSCs were fusiform oriented in the aligned phase and randomly arranged in a paving-stone-like morphology in the random phase. The increased expression of fibroblastic markers demonstrated that only the alignment of nanofibers worked with mechanical stimulation to promote effective fibroblast differentiation. This scaffold was a dense collagenous structure, and there was minimal difference in collagen direction in the orientation phase. Conclusion: Dual-phase electrospun scaffolds had mechanical properties and degradability similar to those of ACLs. They promoted differences in the morphology of MSCs and induced fibroblast differentiation under dynamic culture conditions. Animal experiments showed that ligamentous tissue regenerated well and supported joint stability. Background: Replacing damaged anterior cruciate ligaments (ACLs) with tissue-engineered artificial ligaments is challenging because ligament scaffolds must have a multiregional structure that can guide stem cell differentiation. Here, we designed a biphasic scaffold and evaluated its effect on human marrow mesenchymal stem cells (MSCs) under dynamic culture conditions as well as rat ACL reconstruction model in vivo. Methods: We designed a novel dual-phase electrospinning strategy wherein the scaffolds comprised randomly arranged phases at the two ends and an aligned phase in the middle. The morphological, mechanical properties and scaffold degradation were investigated. MSCs proliferation, adhesion, morphology and fibroblast markers were evaluated under dynamic culturing. This scaffold were tested if they could induce ligament formation using a rodent model in vivo. Results: Compared with other materials, poly(D,L-lactide-co-glycolide)/poly(ε-caprolactone) (PLGA/PCL) with mass ratio of 1:5 showed appropriate mechanical properties and biodegradability that matched ACLs. After 28 days of dynamic culturing, MSCs were fusiform oriented in the aligned phase and randomly arranged in a paving-stone-like morphology in the random phase. The increased expression of fibroblastic markers demonstrated that only the alignment of nanofibers worked with mechanical stimulation to promote effective fibroblast differentiation. This scaffold was a dense collagenous structure, and there was minimal difference in collagen direction in the orientation phase. Conclusion: Dual-phase electrospun scaffolds had mechanical properties and degradability similar to those of ACLs. They promoted differences in the morphology of MSCs and induced fibroblast differentiation under dynamic culture conditions. Animal experiments showed that ligamentous tissue regenerated well and supported joint stability.

Patient and Care Delays of Breast Cancer in China

Yue-Lin Li,Ya-Chao Qin,Lu-Ying Tang,Yu-Huang Liao,Wei Zhang,Xiao-Ming Xie,Qiang Liu,Ying Lin,Ze-Fang Ren 대한암학회 2019 Cancer Research and Treatment Vol.51 No.3

Purpose This study differentiates patient and care delays of breast cancer and explores the related factors as well as the associations with the prognosis in Guangzhou, a southern city of China. Materials and Methods A cohort of female incident breast cancer patients (n=1,551) was recruited from October 2008 to March 2012 and followed up until January 1, 2016 (n=1,374) in the affiliated hospitals of Sun Yat-sen University. The factors associated with patient and care delays were analyzed with multivariable logistic models. Cox proportional hazards regression models were constructed to estimate the impacts of the delays on the prognosis. Results There were 40.4% patient delay (! 3 months) and 15.5% care delay (! 1 month). The patient delay, but not the care delay, was significantly related to the clinical stage and consequently worsened the prognosis of breast cancer (hazard ratio, 1.45; 95% confidence interval, 1.09 to 1.91 for progression-free survival). The factors related to an increased patient delay included premenopausal status, history of benign breast disease, and less physical examination. Conclusion Patient delay was the main type of delay in Guangzhou and resulted in higher clinical stage and poor prognosis of breast cancer. Screening for breast cancer among premenopausal women may be an effective way to reduce this delay.