The purified extract of steamed Panax ginseng protects cardiomyocyte from ischemic injury via caveolin-1 phosphorylation-mediating calcium influx

Hai-Xia Li,Yan Ma,Yu-Xiao Yan,Xin-Ke Zhai,Meng-Yu Xin,Tian Wang,Dong-Cao Xu,Yu-Tong Song,Chun-Dong Song,Cheng-Xue Pan 고려인삼학회 2023 Journal of Ginseng Research Vol.47 No.6

Background: Caveolin-1, the scaffolding protein of cholesterol-rich invaginations, plays an important rolein store-operated Ca2þ influx and its phosphorylation at Tyr14 (p-caveolin-1) is vital to mobilize protectionagainst myocardial ischemia (MI) injury. SOCE, comprising STIM1, ORAI1 and TRPC1, contributesto intracellular Ca2þ ([Ca2þ]i) accumulation in cardiomyocytes. The purified extract of steamed Panaxginseng (EPG) attenuated [Ca2þ]i overload against MI injury. Thus, the aim of this study was to investigatethe possibility of EPG affecting p-caveolin-1 to further mediate SOCE/[Ca2þ]i against MI injury in neonatalrat cardiomyocytes and a rat model. Methods: PP2, an inhibitor of p-caveolin-1, was used. Cell viability, [Ca2þ]i concentration were analyzedin cardiomyocytes. In rats, myocardial infarct size, pathological damages, apoptosis and cardiac fibrosiswere evaluated, p-caveolin-1 and STIM1 were detected by immunofluorescence, and the levels ofcaveolin-1, STIM1, ORAI1 and TRPC1 were determined by RT-PCR and Western blot. And, release of LDH,cTnI and BNP was measured. Results: EPG, ginsenosides accounting for 57.96%, suppressed release of LDH, cTnI and BNP, and protectedcardiomyocytes by inhibiting Ca2þ influx. And, EPG significantly relieved myocardial infarct size, cardiacapoptosis, fibrosis, and ultrastructure abnormality. Moreover, EPG negatively regulated SOCE viaincreasing p-caveolin-1 protein, decreasing ORAI1 mRNA and protein levels of ORAI1, TRPC1 and STIM1. More importantly, inhibition of the p-caveolin-1 significantly suppressed all of the above cardioprotectionof EPG. Conclusions: Caveolin-1 phosphorylation is involved in the protective effects of EPG against MI injury viaincreasing p-caveolin-1 to negatively regulate SOCE/[Ca2þ]i.

공동화 마을의 예술적 재생 - 산동성(山東省) 쯔보시(淄博市) 아장(峨莊) 지역을 대상으로 -

( Yu Dong-ming ),( Xia Tian-tian ),( Kang Tai-ho ) 한국전통조경학회(구 한국정원학회) 2016 Journal of Korean Institute of Traditional Landsca Vol.14 No.-

도시화와 새마을 건설의 물결에 따라 적지 않는 마을에 질적 변화가 발생하고 있다. 공동화 마을은 그중의 하나이다. 본 연구는 공동화 마을의 부정적 효과를 분석하기 위하여 산동성(山東省) 쯔보시(淄博市) 아장(峨莊) 지역을 대상으로 지역의 현황과 특성, 예술적 맥락을 토대로 한 재생 방안을 수행하였다. 연구 결과 날로 공동화가 진행되어 가고 있는 마을의 예술 자원(전통 주택, 거리와 골목, 노거수 등)의 보호 및 발굴, 예술 체험(현장 사생, 예술 작품 감상, 작품 원형 전시 등), 예술가 작업실(미술, 서예, 수석, 뿌리 조각품, 도예, 자기, 마을 민속관 등) 입주, 벽화마을 조성, (사진, 연극, 영화, 드라마)촬영지 홍보, 예술 생활 및 생산품 전시, 마을의 조경 및 정원의 예술화 등을 이용한 마을의 활성화 전략을 모색하여 지역 특색이 있는 마을 조성 방안을 제시하였다. In the process of urbanization and new village construction, a number of villages witnessed qualitative changes. The hollowed village is a representative. Base on the analysis of the negative effects of hollow village, this paper takes the villages in E`zhuang area in Zibo City, Shandong Province as an example and explores the artistic means of revitalization for the villages. With the method of field investigation and in accordance with the regional features and artistic context, some revitalization approaches were proposed. For instance, the protection and exploration of the artistic resources such as traditional residences, streets and ancient trees, the artistic activities including sketching, artistic works appreciation and visiting the archetype in the villages, the foundation of artistic studios of paintings, calligraphy, rocks, root carving, pottery, porcelain, folk museum etc., the construction of mural villages, the advertising of photography, opera, film and television shooting spots, the exhibition of the artistic life and production, and the introduction of landscape to the courtyards, and so on. It aims to build a new village with regional features.

Characterization of a high-thermal-stability spiroanthracenefluorene-based blue-light-emitting polymer optical gain medium

Xia, R.,Heliotis, G.,Campoy-Quiles, M.,Stavrinou, P. N.,Bradley, D. D. C.,Vak, Doojin,Kim, Dong-Yu American Institute of Physics 2005 JOURNAL OF APPLIED PHYSICS - Vol.98 No.8

The Feasibility of Using Biomarkers Derived from Circulating Tumor DNA Sequencing as Predictive Classifiers in Patients with Small-Cell Lung Cancer

Yu Feng,Yutao Liu,Mingming Yuan,Guilan Dong,Hongxia Zhang,Tongmei Zhang,Lianpeng Chang,Xuefeng Xia,Lifeng Li,Haohua Zhu,Puyuan Xing,Hongyu Wang,Yuankai Shi,Zhijie Wang,Xingsheng Hu 대한암학회 2022 Cancer Research and Treatment Vol.54 No.3

Purpose To investigate the feasibility of biomarkers based on dynamic circulating tumor DNA (ctDNA) to classify small cell lung cancer (SCLC) into different subtypes. Materials and Methods Tumor and longitudinal plasma ctDNA samples were analyzed by next-generation sequencing of 1,021 genes. PyClone was used to infer the molecular tumor burden index (mTBI). Pre-treatment tumor tissues [T1] and serial plasma samples were collected (pre-treatment [B1], after two [B2], six [B3] cycles of chemotherapy and at progression [B4]). Results Overall concordance between T1 and B1 sequencing (n=30) was 66.5%, and 89.5% in the gene of <i>RB1</i>. A classification method was designed according to the changes of <i>RB1</i> mutation, named as subtype Ⅰ (both positive at B1 and B2), subtype Ⅱ (positive at B1 but negative at B2), and subtype Ⅲ (both negative at B1 and B2). The median progressive-free survival for subtype Ⅰ patients (4.5 months [95%CI: 2.6-5.8]) was inferior to subtype Ⅱ (not reached, p<0.0001) and subtype Ⅲ (10.8 months [95%CI: 6.0-14.4], p=0.002). The median overall survival for subtype Ⅰ patients (16.3 months [95%CI: 5.3-22.9]) was inferior to subtype Ⅱ (not reached, p=0.01) and subtype Ⅲ (not reached, p=0.02). Patients with a mTBI dropped to zero at B2 had longer median overall survival (not reached vs. 19.5 months, p=0.01). The changes of mTBI from B4 to B1 were sensitive to predict new metastases, with a sensitivity of 100% and a specificity of 85.7%. Conclusion Monitoring ctDNA based <i>RB1</i> mutation and mTBI provided a feasible tool to predict the prognosis of SCLC.

The Transformation Coding with Estimation in the Domain of Frequency

Dong Han Jin,Qing Chen,Xia Nao Zhang,Xin Yu Huang 대한전자공학회 1991 International Conference on Electronics, Informati Vol.1 No.1

Effects of a traditional Chinese medicine formula and its extraction on muscle fiber characteristics in finishing pigs, porcine cell proliferation and isoforms of myosin heavy chain gene expression in myocytes

Yu, Qin Ping,Feng, Ding Yuan,He, Xiao Jun,Wu, Fan,Xia, Min Hao,Dong, Tao,Liu, Yi Hua,Tan, Hui Ze,Zou, Shi Geng,Zheng, Tao,Ou, Xian Hua,Zuo, Jian Jun Asian Australasian Association of Animal Productio 2017 Animal Bioscience Vol.30 No.11

Objective: This study evaluated the effects of a traditional Chinese medicine formula (TCMF) on muscle fiber characteristics in finishing pigs and the effects of the formula's extract (distilled water, ethyl acetate and petroleum ether extraction) on porcine cell proliferation and isoforms of myosin heavy chain (MyHC) gene expression in myocytes. Methods: In a completely randomized design, ninety pigs were assigned to three diets with five replications per treatment and six pigs per pen. The diets included the basal diet (control group), TCMF1 (basal diet+2.5 g/kg TCMF) and TCMF2 (basal diet+5 g/kg TCMF). The psoas major muscle was obtained from pigs at the end of the experiment. Muscle fiber characteristics in the psoas major muscle were analyzed using myosin ATPase staining. Cell proliferation was measured using 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) dye and cytometry. Isoforms of MyHC gene expression were detected by real-time quantitative polymerase chain reaction. Results: The final body weight and carcass weight of finishing pigs were increased by TCMF1 (p<0.05), while the psoas major muscle cross-sectional area was increased by TCMF (p<0.05). The cross-sectional area and diameter of psoas major muscle fiber Ι, IIA, and IIB were increased by TCMF2 (p<0.05). The cross-sectional area and fiber diameter of psoas major muscle fiber IIA and IIB were increased by diet supplementation with TCMF1 (p<0.05). Psoas major muscle fiber IIA and IIB fiber density from the pigs fed the TCMF1 diet and the type IIB fiber density from the pigs fed the TCMF2 diet were lower compared to pigs fed the control diet (p<0.05). Pigs fed TCMF2 had a higher composition of type Ι fiber and a lower percentage of type IIB fiber in the psoas major muscle (p<0.05). The expression levels of MyHC Ι, MyHC IIa, and MyHC IIx mRNA increased and the amount of MyHC IIb mRNA decreased in the psoas major muscle from TCMF2, whereas MyHC Ι and MyHC IIx mRNA increased in the psoas major muscle from TCMF1 (p<0.05). Peroxisome proliferator-activated receptor ${\gamma}$ $coactivator-1{\alpha}$ and CaN mRNA expression in the psoas major muscle were up-regulated by TCMF (p<0.05). Porcine skeletal muscle satellite cell proliferation was promoted by $4{\mu}g/mL$ and $20{\mu}g/mL$ TCMF water extraction (p<0.05). Both $1{\mu}g/mL$ and $5{\mu}g/mL$ of TCMF water extraction increased MyHC IIa, MyHC IIb, and MyHC IIx mRNA expression in porcine myocytes (p<0.05), while MyHC Ι mRNA expression in porcine myocytes was decreased by $5{\mu}g/mL$ TCMF water extraction (p<0.05). Porcine myocyte MyHC Ι and MyHC IIx mRNA expression were increased, and MyHC IIa and MyHC IIb mRNA expression were down-regulated by $5{\mu}g/mL$ TCMF ethyl acetate extraction (p<0.05). MyHC Ι and MyHC IIa mRNA expression in porcine myocytes were increased, and the MyHC IIb mRNA expression was decreased by $1{\mu}g/mL$ TCMF ethyl acetate extraction (p<0.05). Four isoforms of MyHC mRNA expression in porcine myocytes were reduced by $5{\mu}g/mL$ TCMF petroleum ether extraction (p<0.05). MyHC IIa mRNA expression in porcine myocytes increased and MyHC IIb mRNA expression decreased by $1{\mu}g/mL$ in a TCMF petroleum ether extraction (p<0.05). Conclusion: These results indicated that TCMF amplified the psoas major muscle cross-sectional area through changing muscle fiber characteristics in finishing pigs. This effect was confirmed as TCMF extraction promoted porcine cell proliferation and affected isoforms of MyHC gene expression in myocytes.

An Improved SPWM Strategy to Reduce Switching in Cascaded Multilevel Inverters

Dong, Xiucheng,Yu, Xiaomei,Yuan, Zhiwen,Xia, Yankun,Li, Yu The Korean Institute of Power Electronics 2016 JOURNAL OF POWER ELECTRONICS Vol.16 No.2

The analysis of the switch status of each unit module of a cascaded multi-level inverter reveals that the working condition of the switch of a chopper arm causes unnecessary switching under the conventional unipolar sinusoidal pulse width modulation (SPWM). With an increase in the number of cascaded multilevel inverters, the superposition of unnecessary switching gradually occurs. In this work, we propose an improved SPWM strategy to reduce switching in cascaded multilevel inverters. Specifically, we analyze the switch state of the switch tube of a chopper arm of an H-bridge unit. The redundant switch is then removed, thereby reducing the switching frequency. Unlike the conventional unipolar SPWM technique, the improved SPWM method greatly reduces switching without altering the output quality of inverters. The conventional unipolar SPWM technique and the proposed method are applied to a five-level inverter. Simulation results show the superiority of the proposed strategy. Finally, a prototype is built in the laboratory. Experimental results verify the correctness of the proposed modulation strategy.

Effect of Portal Vein Chemotherapy on Liver Metastasis after Surgical Resection of Colorectal Cancer

Yu, Dong-Sheng,Li, Ying,Huang, Xin-En,Lu, Yan-Yan,Wu, Xue-Yan,Liu, Jin,Cao, Jie,Xu, Xia,Xiang, Jin,Wang, Guo-Ping Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.9

Objective: To explore the effect of portal vein chemotherapy on liver metastasis after surgical resection of colorectal cancer. Methods: Patients fulfilling the eligibility criteria were assigned to receive either surgery plus 1-week continuous infusion of 5-FU (study group) or surgery alone (observational group). Patients in the study group received portal vein chemotherapy, whereby 5-FU (1000 mg/d) and heparin (5000 IU/d) infusion was initiated from the day of surgery and lasted for 7 consecutive days. Liver metastasis was monitored during five years follow-up postoperatively. Results: Sixty four patients were recruited and assigned to the study group (12 with colon and 20 with rectal cancer) or the control group (10 with colon and 22 with rectal cancer). Liver metastasis rate was 12.5% in study and 25.0% in observational group, the difference being significant (P<0.01). Conclusion: Portal vein chemotherapy could be an effective treatment in preventing liver metastasis after surgical resection of colorectal cancer.

Preparation and characterization of a novel hydrophilic poly(vinylidene fluoride) filtration membrane incorporated with Zn–Al layered double hydroxides

Yu Zhao,Nana Li,Bin Xu,Bingzhi Dong,Shengji Xia 한국공업화학회 2016 Journal of Industrial and Engineering Chemistry Vol.39 No.-

Hybrid poly(vinylidene fluoride) (PVDF) embedded with Zn–Al layered double hydroxides (LDH)membranes were fabricated via phase inversion. The physical and chemical properties of the novelpolymer membranes were characterized using different characterizations. Filtration and antifoulingexperiments were performed to examine the water permeability, rejection and antifouling ability of theprepared membranes. Results showed that membrane morphology was greatly changed due to theaddition of LDH and that the hybrid membrane exhibited greatly improved surface hydrophilicity, waterpermeability and antifouling property, with contact angle decreased from 76.58 to 69.08 and pure water(PW) flux increased from 8.01 L/m2 h bar to 48.95 L/m2 h bar.

FBW7 Upregulation Enhances Cisplatin Cytotoxicity in Non-small Cell Lung Cancer Cells

Yu, Hao-Gang,Wei, Wei,Xia, Li-Hong,Han, Wei-Li,Zhao, Peng,Wu, Sheng-Jun,Li, Wei-Dong,Chen, Wei Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.11

Introduction: Lung cancer is extremely harmful to human health and has one of the highest worldwide incidences of all malignant tumors. Approximately 80% of lung cancers are classified as non-small cell lung cancers (NSCLCs). Cisplatin-based multidrug chemotherapy regimen is standard for such lesions, but drug resistance is an increasing problem. F-box/WD repeat-containing protein 7 (FBW7) is a member of the F-box protein family that regulates cell cycle progression, and cell growth and differentiation. FBW7 also functions as a tumor suppressor. Methods: We used cell viability assays, Western blotting, and immunofluorescence combined with siRNA interference or plasmid transfection to investigate the underlying mechanism of cisplatin resistance in NSCLC cells. Results: We found that FBW7 upregulation significantly increased cisplatin chemosensitivity and that cells expressing low levels of FBW7, such as NCI-H1299 cells, have a mesenchymal phenotype. Furthermore, siRNA-mediated silencing or plasmid-mediated upregulation of FBW7 resulted in altered epithelial-mesenchymal transition (EMT) patterns in NSCLC cells. These data support a role for FBW7 in regulating the EMT in NSCLC cells. Conclusion: FBW7 is a potential drug target for combating drug resistance and regulating the EMT in NSCLC cells.