Sublimable materials facilitate the TEM sample preparation of oil-soluble nanomaterials

Yu-Hao Deng 한국현미경학회 2020 Applied microscopy Vol.50 No.1

Sample preparation is significantly important to the high-resolution transmission electron microscopy (HRTEM) characterization of nanomaterials. However, many general organic solvents can dissolve the necessary organic polymer support layer in TEM grid, which causes it difficult to obtain high-quality samples of oil-soluble nanomaterials. In this study, a new sample preparation method for oil-soluble nanomaterials has been developed by using the sublimable material as a transition layer. Experiments also show that there is no damage to TEM grids and high-quality HRTEM images can be obtained via this method. This approach paves the way to applicable HRTEM sample preparation of oil-soluble nanomaterials.

Growth of centimeter-scale perovskite single-crystalline thin film via surface engineering

Deng Yu-Hao,Yang Zhen-Qian,Ma Ren-Min 나노기술연구협의회 2020 Nano Convergence Vol.7 No.25

Modern electronic and photonic devices rely on single-crystalline thin film semiconductors for high performance and reproducibility. The emerging halide perovskites have extraordinary electronic and photonic properties and can be synthesized via low cost solution-based methods. They have been used in a variety of devices with performance approaching or over the devices based on conventional materials. However, their solution based growth method is intrinsically challenge to grow large scale single-crystalline thin film due to the random nucleation and isotropous growth of the crystal. Here, we report the growth of centimeter-scale perovskite single-crystalline thin films by con‑ trolling the nucleation density and growth rate of the crystal under a spatially confined growth condition. The hydro‑ phobic treatment on substrates inhibits nucleation and accelerates the growth of single-crystalline thin film, provid‑ ing enough space for initial nucleus growing up quickly without touching each other. Single-crystalline perovskite thin-film with an aspect ratio of 1000 (1 cm in side length, 10 μm in thickness) has been successfully grown. The low trap density and the high mobility of the as-grown thin film show a high crystallinity. The photodetector based on the perovskite thin film has achieved a gain ~ 10 4 , benefitting from the short transit time of the carries due to the high mobility and thin thickness of the active layer. Our work opens up a new route to grow large scale perovskite singlecrystalline thin films, providing a platform to develop high- performance devices.

Hsa-miRNA-143-3p Reverses Multidrug Resistance of Triple-Negative Breast Cancer by Inhibiting the Expression of Its Target Protein Cytokine-Induced Apoptosis Inhibitor 1 In Vivo

Yu Wei Deng,Wen Jing Hao,Yi Wen Li,Yi Xin Li,Bo Chen Zhao,Dan Lu 한국유방암학회 2018 Journal of breast cancer Vol.21 No.3

Purpose: Multidrug resistance (MDR) remains a major obstacle in the treatment of triple-negative breast cancer (TNBC) with conventional chemotherapeutic agents. A previous study demonstrated that hsa-miRNA-143-3p plays a vital role in drug resistance of TNBC. Downregulation of hsa-miRNA-143-3p upregulated the expression of its target protein cytokine-induced apoptosis inhibitor 1 (CIAPIN1) in order to activate MDR, while upregulation of hsa-miRNA-143-3p effectively enhances the sensitivity of drug-resistant TNBC cells to chemotherapeutics. The present study aimed to further verify these findings in vivo. Methods: We established a hypodermic tumor nude mice model using paclitaxel- resistant TNBC cells. We expressed ectopic hsa-miRNA- 143-3p under the control of a breast cancer-specific human mammaglobin promoter that guided the efficient expression of exogenous hsa-miRNA-143-3p only in breast cancer cells. Thereafter, we overexpressed hsa-miRNA-143-3p in xenografts using a recombinant virus system and quantified the expression of hsa-miRNA-143-3p, CIAPIN1 protein, and proteins encoded by related functional genes by western blot. Results: We successfully completed the prospective exploration of the intravenous virus injection pattern from extensive expression to targeted expression. The overexpression of hsa-miRNA-143-3p significantly alleviated chemoresistance of TNBC by inhibiting viability. In addition, we observed that the expression of CIAPIN1 as a hsa-miRNA-143-3p target protein was remarkably decreased. Conclusion: We partly illustrated the mechanism underlying the hsa-miRNA-143-3p/CIAPIN1 drug resistance pathway. HsamiRNA- 143-3p as a tumor suppressive microRNA may be a novel target to effectively reverse MDR of TNBC in vivo.

Network Analysis of microRNAs, Genes and their Regulation in Mantle Cell Lymphoma

Deng, Si-Yu,Guo, Xiao-Xin,Wang, Ning,Wang, Kun-Hao,Wang, Shang Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.2

The pathogenesis of mantle cell lymphoma, a special subtype of lymphoma that is invasive and indolent and has a median survival of 3 to 4 years, is still partially unexplained. Much research about genes and miRNAs has been conducted in recent years, but interactions and regulatory relations of genetic elements which may play a vital role in genesis of MCL have attracted only limited attention. The present study concentrated on regulatory relations about genes and miRNAs contributing to MCL pathogenesis. Numerous experimentally validated raw data were organized into three topology networks, comprising differentially expressed, associated and global examples. Comparison of similarities and dissimilarities of the three regulating networks, paired with the analysis of the interactions between pairs of elements in every network, revealed that the differentially expressed network illuminated the carcinogenicity mechanism of MCL and the related network further described the regulatory relations involved, including prevention, diagnosis, development and therapy. Three kinds of regulatory relations for host genes including miRNAs, miRNAs targeting genes and genes regulating miRNAs were concluded macroscopically. Regulation of the differentially expressed miRNAs was also analyzed, in terms of abnormal gene expression affecting the MCL pathogenesis. Special regulatory relations were uncovered. For example, auto-regulatory loops were found in the three topology networks, key pathways of the nodes being highlighted. The present study focused on a novel point of view revealing important influencing factors for MCL pathogenesis.

Molecular characterization of Japanese encephalitis virus strains prevalent in Chinese swine herds

Hao Zheng,Tongling Shan,Yu Deng,Chunqing Sun,Shishan Yuan,Yang Yin,Guangzhi Tong 대한수의학회 2013 JOURNAL OF VETERINARY SCIENCE Vol.14 No.1

Japanese encephalitis virus (JEV) is the leading cause of viral encephalitis in Asia and domestic pigs serve as the amplifying hosts. In the present study, the full genomic sequences of two JEV strains (HEN0701 and SH0601) isolated from pigs in China were determined and compared with other 12 JEV strains deposited in GenBank. These two strains had an 88.8% nucleotide sequence similarity and 97.9% deduced amino acid sequence homology. HEN0701 had high nucleotide sequence and high amino acid sequence identity with genotype I (GI) strains, while SH0601 had high nucleotide sequence and high amino acid sequence identity with GIII strains at both the gene and full genome levels. Further phylogenetic analysis showed that HEN0701 belonged to the JEV GI group and SH0601 was classified as a GIII strain. Analysis of codon usage showed there were a few differences between the GI and GIII strains in nucleotide composition and codon usage for the open reading frames.

Hierarchically magnetic Ni–Al binary layered double hydroxides: towards tunable dual electro/magneto-stimuli performances

Li Deng,Wenpeng Jia,Wen Zheng,Hao Liu,Degang Jiang,Zimeng Li,Yu Tian,Wenling Zhang,Jingquan Liu 한국공업화학회 2018 Journal of Industrial and Engineering Chemistry Vol.58 No.-

Ni–Al layered double hydroxides (Ni–Al LDHs) are adopted as an electrorheological (ER) materialascribed to their special layered structure, ample polarizable groups and good dispersibility. Themagnetite nanoparticles (Fe3O4 NPs) are utilized as classical magnetorheological (MR) materials onaccount of their excellent paramagnetic property. In this work, we report the rational design of highperformanceER/MR materials by integrating the structural merits of Fe3O4 NPs onto a binary layeredstructure system. The obtained Fe3O4/Ni–Al LDH NCs dispersed in silicone oil displayed appealing dualER/MR performances. The work opens a new path toward novel stimuli-responsive materials forindustrial applications.

Acute Myelitis in a Patient with Vogt-Koyanagi-Harada Disease: Case Report and Review of the Literature

Shaojuan GU,Yu Liu,Xiaohong Zi,Hao Deng 대한신경과학회 2013 Journal of Clinical Neurology Vol.9 No.1

Background Vogt-Koyanagi-Harada (VKH) disease is characterized by bilateral granulomatous uveitis with neurologic, auditory, and dermatologic manifestations. However, acute myelitis complicating VKH disease has rarely been reported. Case Report A 50-year-old Chinese Han woman presented with difficulty walking, numbness on the left side of the body, and difficulty with urination. The patient was diagnosed with incomplete VKH disease and received corticosteroid treatment prior to the neurological presentation. Acute myelitis was diagnosed based on both clinical and spinal-cord MRI findings. Conclusions Clinicians should consider acute myelitis as a rare possible neurological manifestation in VKH disease patients, and early systemic administration of corticosteroids will suppress the acute inflammatory process and prevent recurrences. This report raises the possibility that VKH disease and acute myelitis share common pathogenic pathways

Traditional Chinese Medicine as a Remedy for Male Infertility: A Review

Shao Hu Zhou,Yu Fei Deng,Zhi Wei Weng,Hao Wei Weng,Zhi Dan Liu 대한남성과학회 2019 The World Journal of Men's Health Vol.37 No.2

Male infertility (MI) is a complex multifactorial disease, and idiopathic infertility accounts for 30% of cases of MI. At present, the evidence for the effectiveness of empirical drugs is limited, and in vitro fertilization is costly and may increase the risk of birth defects and childhood cancers. Therefore, affected individuals may feel obliged to pursue natural remedies. Traditional Chinese medicine (TCM) may represent a useful option for infertile men. It has been demonstrated that TCM can regulate the hypothalamic-pituitary-testicular axis and boost the function of Sertoli cells and Leydig cells. TCM can also alleviate inflam-mation, prevent oxidative stress, reduce the DNA fragmentation index, and modulate the proliferation and apoptosis of germ cells. Furthermore, TCM can supply trace elements and vitamins, ameliorate the microcirculation of the testis, decrease the levels of serum anti-sperm antibody, and modify epigenetic markers. However, the evidence in favor of TCM is not compel-ling, which has hindered the development of TCM. This review attempts to elucidate the underlying therapeutic mechanisms of TCM. We also explore the advantages of TCM, differences between TCM and Western medicine, and problems in existing studies. Subsequently, we propose solutions to these problems and present perspectives for the future development of TCM.

Bioinspired Hierarchical Layer-within-network Structure of Polymer Nanocomposites for High-performance All-solid-state Flexible Supercapacitors

Wei-Tsung Chuang,Rong-Hao Guo,Che-Min Chou,Chun-Chieh Wang,Ming-Jay Deng,Jhih-Min Lin,Chun-Yu Chen,Yao-Chang Lee 한국고분자학회 2021 한국고분자학회 학술대회 연구논문 초록집 Vol.46 No.2

To meet future demands for cutting-edge wearable electronics, flexible supercapacitors must possess many features, such as eco-friendly processing, aesthetic appeal and no health hazards, in addition to have lightweight, robust and excellent cycling stability. We proposed a biomimetic and scalable method to fabricate an all-solid-state flexible supercapacitor (assFSC) using bioinspired clay/polymer nanocomposites as electrode materials and a gel electrolyte. Experimental results from X-ray techniques (tomography, scattering and diffraction) showed that the electrode’s structure features a 3D ant-nest-like framework composed of 2D nacre-like clay nanosheets, i.e. hierarchical layers-within-networks structure. Accordingly, the structural electrodes exhibit high tensile strength of 62 MPa, Young’s modulus of 4.4 GPa, and torsional strength of 165 MPa. Under a large operating potential of 4.0 V, the assFSC exhibited ultrahigh energy density (233.3 W h kg<SUP>-1</SUP> at 2 kW kg<SUP>-1</SUP>), ultrahigh power density (125 kW kg<SUP>-1</SUP> at 55.5 W h kg<SUP>-1</SUP>), and outstanding static cyclability (less than 10% loss after 5,000 cycles). We also performed a cycle-life test under dynamic deformation and demonstrated that the assFSC had charging and discharging abilities during motion, according to particle applications of wearable electronics. Thus stable and superior electrochemical performance can be attributed to the biomimetic layers-within-networks structure, which not only provided robust framework but also induced 3D conducting networks with increasing ion channels and shortening charge transports. The shapeable electrodes made by a molding process could, therefore, be used to meet the demands for fashionable, wearable electronics.

폴리프로필렌/폴리아미드 엘라스토머 블렌드

Qing Sheng Liu,Yan Xu,Hong Xia Zhang,Yu Hao Li,Bing Yao Deng 한국고분자학회 2014 폴리머 Vol.38 No.5

The polypropylene/polyamide elastomer (PP/PAE) blends were prepared by melt mixing. PP and PAE in PP/PAE were immiscible completely. The size of PAE domains was large and the clear gap in the interface between PP and PAE existed, which did not meet the conditions enhancing toughness of polymers by elastomer. Therefore, maleic anhydride grafted polypropylene (MP) was used to improve the miscibility between PP and PAE. The miscibility between PP and PAE was improved and the size of dispersed phase PAE decreased by introducing MP. The crystallization of PP became easier by introducing PAE as a nucleating agent. With the increase of PAE content, the melt-crystallization temperatures of PP components in PP/PAE/MP blends increased gradually. The melt-crystallization of the polytetramethyleneoxide segment of PAE component in PP/PAE blends were hampered by PP component. In addition, PAE can enhance significantly the toughness of PP, and the tensile strength and modulus did not decrease.