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Qi Wenlingli,Xue Ming-Yuan,Jia Ming-Hui,Zhang Shuxian,Yan Qiongxian,Sun Hui-Zeng 아세아·태평양축산학회 2024 Animal Bioscience Vol.37 No.2
Rumen microbiota play a central role in the digestive process of ruminants. Their remarkable ability to break down complex plant fibers and proteins, converting them into essential organic compounds that provide animals with energy and nutrition. Research on rumen microbiota not only contributes to improving animal production performance and enhancing feed utilization efficiency but also holds the potential to reduce methane emissions and environmental impact. Nevertheless, studies on rumen microbiota face numerous challenges, including complexity, difficulties in cultivation, and obstacles in functional analysis. This review provides an overview of microbial species involved in the degradation of macromolecules, the fermentation processes, and methane production in the rumen, all based on cultivation methods. Additionally, the review introduces the applications, advantages, and limitations of emerging omics technologies such as metagenomics, metatranscriptomics, metaproteomics, and metabolomics, in investigating the functionality of rumen microbiota. Finally, the article offers a forward-looking perspective on the new horizons and technologies in the field of rumen microbiota functional research. These emerging technologies, with continuous refinement and mutual complementation, have deepened our understanding of rumen microbiota functionality, thereby enabling effective manipulation of the rumen microbial community.
Xuqiang Ji,Wenling Zhang,Wenpeng Ji,Xiaoxia Wan,Yutian Wang,Li Deng,Jingquan Liu 한국공업화학회 2017 Journal of Industrial and Engineering Chemistry Vol.56 No.-
The cactus-like double-shell structured SiO2@TiO2 microspheres (CDS SiO2@TiO2 microspheres) withhigh dielectric properties were successfully fabricated and utilized as high-performance electrorheological(ER)fluids and microwave absorbing materials. The SiO2 nanospheres with average diametersof 190 nm were synthesized by the hydrolysis of tetraethyl orthosilicate (TEOS), and used as the core forthefirst coating layer of TiO2 achieved by sol-gel method. Thefinal cactus-like TiO2 layer was prepared viaa hydrothermal method. The morphology of the resultant samples was characterized via transmissionelectron microscopy (TEM), scanning electron microscopy (SEM) and scanning transmission electronmicroscopy (STEM). This novel CDS SiO2@TiO2 microspheres based suspension exhibited improved ERproperties compared to conventional bare SiO2 spheres.
Microarrays for the Detection of HBV and HDV
Sun, Zhaohui,Zheng, Wenling,Zhang, Bao,Shi, Rong,Ma, Wenli Korean Society for Biochemistry and Molecular Biol 2004 Journal of biochemistry and molecular biology Vol.37 No.5
The increasing pace of development in molecular biology during the last decade has had a direct effect on mass testing and diagnostic applications, including blood screening. We report the model Microarray that has been developed for Hepatitis B virus (HBV) and Hepatitis D virus (HDV) detection. The specific primer pairs of PCR were designed using the Primer Premier 5.00 program according to the conserved regions of HBV and HDV. PCR fragments were purified and cloned into pMD18-T vectors. The recombinant plasmids were extracted from positive clones and the target gene fragments were sequenced. The DNA microarray was prepared by robotically spotting PCR products onto the surface of glass slides. Sequences were aligned, and the results obtained showed that the products of PCR amplification were the required specific gene fragments of HBV, and HDV. Samples were labeled by Restriction Display PCR (RD-PCR). Gene chip hybridizing signals showed that the specificity and sensitivity required for HBV and HDV detection were satisfied. Using PCR amplified products to construct gene chips for the simultaneous clinical diagnosis of HBV and HDV resulted in a quick, simple, and effective method. We conclude that the DNA microarray assay system might be useful as a diagnostic technique in the clinical laboratory. Further applications of RD-PCR for the sample labeling could speed up microarray multi-virus detection.
Tan, Yu,Luo, Shilong,Li, Dongmei,Zhang, Nan,Jia, Shiqi,Liu, Yidong,Qin, Wenling,Song, Choong Eui,Yan, Hailong American Chemical Society 2017 JOURNAL OF THE AMERICAN CHEMICAL SOCIETY - Vol.139 No.18
<P>Structural motifs containing contiguous halide bearing stereocenters are common in natural products as well as bioactive molecules. A few successful examples have been reported in the area of asymmetric vicinal dihalogenation of alkenes for accessing dihalogenated products; in this report, an alternative generation method of contiguous halide-bearing stereocenters alpha,beta,gamma,delta relative to carbonyl group in excellent enantioselectivity is proposed by utilizing a Song's oligoEG catalyst-catalyzed asymmetric beta-elimination. According to this methodology, a wide range of anti-syn-trihalides and anti-syn-anti-tetrahalides with high levels of enantioselectivity were synthesized. The synthetic utility of the contiguous halide-bearing stereocenters was demonstrated by several transformations. The results of high-resolution mass spectrometry indicated that the favorable interaction between catalyst and one of the enantiomers of racemic contiguously multihalogenated ketone contributed to the original enantioselectivity of dehydrohalogenation. A deuterium kinetic isotope effect experiment revealed that this beta-elimination reaction proceeds by the E2 mechanism. This strategy opens a new pathway for the asymmetric synthesis of contiguous halide bearing stereocenters of great complexity.</P>
Plate, Markus,Li, Ting,Wang, Yu,Mo, Xiaoning,Zhang, Yingmei,Ma, Dalong,Han, Wenling Korean Society for Molecular and Cellular Biology 2010 Molecules and cells Vol.29 No.4
Human CMTM is a novel gene family consisting of CKLF and CMTM1-8. CMTM4 is the most conserved gene and has three RNA splicing forms designated as CMTM4-v1, -v2 and -v3, but in many types of tissue and cell lines, only CMTM4-v1 and -v2 could be detected. CMTM4-v2 is the full length cDNA product, which has been highly conserved during e volution. CMTM4-v1 and -v2 a re broadly expressed in normal types of tissue. They are distributed on the cell membrane and across the cytoplasm in a speckled pattern. Overexpression of CMTM4-v1 and -v2 can inhibit HeLa cell growth via G2/M phase accumulation without inducing apoptosis. Therefore, CMTM4 might be an important gene involved in cell growth and cell cycle regulation.
Markus Plate,Ting Li,Yu Wang,Xiaoning Mo,Yingmei Zhang,Dalong Ma,Wenling Han 한국분자세포생물학회 2010 Molecules and cells Vol.29 No.4
Human CMTM is a novel gene family consisting of CKLF and CMTM1-8. CMTM4 is the most conserved gene and has three RNA splicing forms designated as CMTM4-v1, -v2 and -v3, but in many types of tissue and cell lines, only CMTM4-v1 and -v2 could be detected. CMTM4-v2 is the full length cDNA product, which has been highly conserved during evolution. CMTM4-v1 and -v2 are broadly ex-pressed in normal types of tissue. They are distributed on the cell membrane and across the cytoplasm in a speckled pattern. Overexpression of CMTM4-v1 and -v2 can inhibit HeLa cell growth via G2/M phase accumulation without inducing apoptosis. Therefore, CMTM4 might be an impor-tant gene involved in cell growth and cell cycle regulation.
Li Deng,Wenpeng Jia,Wen Zheng,Hao Liu,Degang Jiang,Zimeng Li,Yu Tian,Wenling Zhang,Jingquan Liu 한국공업화학회 2018 Journal of Industrial and Engineering Chemistry Vol.58 No.-
Ni–Al layered double hydroxides (Ni–Al LDHs) are adopted as an electrorheological (ER) materialascribed to their special layered structure, ample polarizable groups and good dispersibility. Themagnetite nanoparticles (Fe3O4 NPs) are utilized as classical magnetorheological (MR) materials onaccount of their excellent paramagnetic property. In this work, we report the rational design of highperformanceER/MR materials by integrating the structural merits of Fe3O4 NPs onto a binary layeredstructure system. The obtained Fe3O4/Ni–Al LDH NCs dispersed in silicone oil displayed appealing dualER/MR performances. The work opens a new path toward novel stimuli-responsive materials forindustrial applications.