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Direct Observation of Nanoscale Pt Electrode Agglomeration at the Triple Phase Boundary
Yu, Chen-Chiang,Kim, Sanwi,Baek, Jong Dae,Li, Yong,Su, Pei-Chen,Kim, Taek-Soo American Chemical Society 2015 ACS APPLIED MATERIALS & INTERFACES Vol.7 No.11
<P>Nanoporous platinum electrode thin films were delaminated from yttria-stabilized zirconia (YSZ) substrates via double cantilever beam delamination to reveal the structure located at the interface between electrode and electrolyte. The thermally driven morphological evolution between the electrode top surface and the substrate contact interface of agglomerated nanoporous platinum thin films were compared. We found the temperature required for significant agglomeration to occur was approximately 100 °C higher at the electrolyte contact interface side than at the top surface side. Judging the reaction active site from the electrode top surface could be inaccurate because higher resistance of thermal agglomeration at the interface could retain the reaction active site during fuel cell operation.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/aamick/2015/aamick.2015.7.issue-11/acsami.5b00134/production/images/medium/am-2015-00134s_0006.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/am5b00134'>ACS Electronic Supporting Info</A></P>
쓴메밀 종자의 추출방법에 따른 루틴 및 퀘세틴 함량 비교
김수정(Su Jeong Kim),손황배(Hwang Bae Sohn),김금희(Geum Hee Kim),이유영(Yu Young Lee),홍수영(Su Young Hong),김기덕(Ki Deog Kim),남정환(Jeong Hwan Nam),장동칠(Dong Chil Chang),서종택(Jong Taek Suh),구본철(Bon Cheol Koo),김율호(Yul Ho 한국식품과학회 2017 한국식품과학회지 Vol.49 No.3
본 연구는 초고성능액체크로마토그래피(Ultra Performance Liquid Chromatography, UPLC)를 이용하여 쓴메밀 종자에서 루틴 및 퀘세틴 함량을 신속 정확히 평가하기 위한 효율적인 추출방법을 구명하고자 수행하였다. 추출방법으로 환류냉각추출, 초음파추출, 상온교반추출, 초음파후 환류냉각추출, 상온교반 후 환류냉각추출, 상온교반후 초음파추출, 상온교반과 초음파 후 환류냉각추출 등 7가지 방법을 비교하였다. 루틴 함량에서 7가지 추출방법은 통계적으로 유의한 차이를 보였으나, 퀘세틴 함량에서 추출방법 간 유의차가 없었다. 환류냉각추출의 루틴과 퀘세틴 함량은 각각 2,277, 158 mg/100 g으로 추출방법 중 함량이 가장 많아 추출수율이 높았으며 검출횟수도 가장 높았다. 반면, 초음파 추출의 루틴과 퀘세틴의 함량이 처리구 중 가장 낮았으며, 검출횟수도 가장 적었다. 환류냉각추출에서 일내 반복성(intra-day repeatablility)과 일간 정밀성(inter-day precision)은 상대적표준편차(relative standard deviation, RSD)가 0.4-3.2%로 안정적으로 추출되었고, 정확도는 88.8-102.4%로 국제적 회수율 권고치 기준에 부합하였다. 따라서, 쓴메밀 루틴 추출방법으로는 추출 소요시간이 1시간 이내로 짧으면서 루틴 함량이 가장 안정적으로 많이 추출되는 환류냉각추출이 가장 적합하였다. The stability and accuracy of ultra-performance liquid chromatography (UPLC) used for evaluating the contents of rutin and quercetin in tartary buckwheat (Fagopyrum tataricum Gaertn.) seeds extracted by seven different extraction methods were determined. The seven extraction methods were reflux extraction (RE), ultra-sonification extraction (UE), stirrer extraction (SE), RE after UE (UE+RE), RE after SE (SE+RE), UE after SE (SE+UE), and RE with UE after SE (SE+UE+RE). Among the seven extraction methods used, RE yielded comparatively higher contents of rutin (2,277 mg/100 g) and quercetin (158 mg/100 g) than those yielded by other six extraction methods. The intra-day repeatability and inter-day precision of RE was 0.4-3.2% considering relative standard deviation (RSD), while accuracy was 88.8-102.4%. Therefore, RE with UPLC would be a rapid, accurate, and stable method for analyzing rutin and quercetin contents in tartary buckwheat.
유색미에 관한 연구 : Ⅲ. 유색미 유망계통에 대한 주요 생육 특성
김광수,최윤표,김선택,최현구,정종태,김보경,유지홍,이희봉 충남대학교 농업과학연구소 2007 농업과학연구 Vol.34 No.2
국내외 유색미 수집종을 돌연변이원에 의해 유기된 우수 계통과 수집종간 상호교배에 의해 선발된 CNU 20계통을 파종하여 얻어진 주요 작물학적 특성을 살펴보면 다음과 같다. 1. 간장은 유색 메벼인 CNU126 계통이 대조품종인 동진벼와 같이 가장 컸고 메성인 CNU 128계통에서 가장 작았다. 2. 주당 분얼수는 CNU3, CNU50. CNU56, CNU112 계통에서 가장 많았고 대조품종과 CNU 128 계통에서 10개 미만으로 가장 적게 나타났다. 3. 수장은 육성 계통 중 CNU88 계통에서 가장 길었으나 대조구보다 짧고, 육성계통 중에서는 CNU 126 계통이 가장 짧았다. 4. 주당 영화수는 대조구인 동진벼보다 CNU50 계통에서 2배 이상 많았고, CNU158 계통에서 가장 적게 나타났다. 5. 천립중은 CNU113 계통에서 30g이상으로 대조구와 같이 가장 무거웠고, CNU128 계통에서 20g으로 가장 적었다. 6. 주당 수량은 대조구의 26.6g에 비해 CNU50, CNU112 계통에서 두배정도 높았고, CNU128, CNU158, CNU200 계통에서 가장 낮게 타나났다. Aims of this study were carried out to develop the useful lines induced from mutation and pedigree breeding methods among the collected genetic resources from national and domestic areas. In this study, Stem height of CNU126 line and check among them were high, while CNU128 was lower than other lines. Spiklet length of CNU88 was longer, but that of CNU126 was shorter than check. Number of spiklets per plant of CNU50 among lines have twice time than check. 1,000 grains weight of CNU113 was higher than check. In yield per plant, CNU50 and CNU112 were higher than check, Dongjinbyeo.
Park, Yu Mi,Han, Sun Hyup,Seo, Su Kyung,Park, Kyung Ah,Lee, Won Taek,Lee, Jong Eun Elsevier 2015 cytotherapy Vol.17 No.1
<P>Mesenchymal stromal cells (MSCs) promote functional recovery in central nervous system (CNS) injury. Neuroprotective effects of MSCs are being tested in clinical trials for the treatment of CNS injury; however, the underlying mechanisms remain unclear. Arginine decarboxylase (ADC) is a rate-limiting enzyme of agmatine synthesis and is known to exist in the CNS of mammals. The present study investigated whether transplantation of ADC-overexpressing human MSCs (ADC-hMSCs) after spinal cord injury (SCI) could increase the production of neurotrophic factors and promote cell survival, differentiation, axonal regeneration and the restoration of functional recovery.</P>
Hydrogel-laden paper scaffold system for origami-based tissue engineering
Kim, Su-Hwan,Lee, Hak Rae,Yu, Seung Jung,Han, Min-Eui,Lee, Doh Young,Kim, Soo Yeon,Ahn, Hee-Jin,Han, Mi-Jung,Lee, Tae-Ik,Kim, Taek-Soo,Kwon, Seong Keun,Im, Sung Gap,Hwang, Nathaniel S. National Academy of Sciences 2015 PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF Vol.112 No.50
<P><B>Significance</B></P><P>This work describes an intriguing strategy for the formation of hydrogel-laden multiform structures utilizing paper sheets and suggests a route for trachea tissue engineering. It combines concepts extracted from paper origami, functional thin polymer coating, and thin hydrogel layering on top of the paper scaffolds. A computer-aided design-based lock-and-key arrangement was used for folding the sheets into multiform structures with spatial arrangements. With encapsulating cells in hydrogel-laden paper, the scaffold system was able to deliver biological cues in vivo. In this work, we have successfully applied an origami-based tissue engineering approach to the trachea regeneration model.</P><P>In this study, we present a method for assembling biofunctionalized paper into a multiform structured scaffold system for reliable tissue regeneration using an origami-based approach. The surface of a paper was conformally modified with a poly(styrene-co-maleic anhydride) layer via initiated chemical vapor deposition followed by the immobilization of poly-<SMALL>l</SMALL>-lysine (PLL) and deposition of Ca<SUP>2+</SUP>. This procedure ensures the formation of alginate hydrogel on the paper due to Ca<SUP>2+</SUP> diffusion. Furthermore, strong adhesion of the alginate hydrogel on the paper onto the paper substrate was achieved due to an electrostatic interaction between the alginate and PLL. The developed scaffold system was versatile and allowed area-selective cell seeding. Also, the hydrogel-laden paper could be folded freely into 3D tissue-like structures using a simple origami-based method. The cylindrically constructed paper scaffold system with chondrocytes was applied into a three-ring defect trachea in rabbits. The transplanted engineered tissues replaced the native trachea without stenosis after 4 wks. As for the custom-built scaffold system, the hydrogel-laden paper system will provide a robust and facile method for the formation of tissues mimicking native tissue constructs.</P>