Helicobacter pylori와 대장균의 Shuttle Vector 개발

조명제,이우곤,이상룡,김경희,안영숙,김성희,김현주,류복덕,최여정,윤영혜,백승철,전영석,이광호 경상대학교 유전공학연구소 1997 遺傳工學硏究所報 Vol.16 No.-

In this study, a vehicle vector using cryptic plasmids was constructed for gene transfer in Helicobacter pylori. pHP51(3.9 kb) and pHP489(1.2 kb) were selected for constructing vectors from cryptic plasmid of H. pylori isolates in Korea. The HindⅢ-digestedDNA fragment(1.2kb) of pHP489 and 1.6kb DNA fragment of pHP51 were ligated with a kanamycin resistance gene(aph3'-Ⅲ) from C. jejuni to produce the recombinant plasmids pHP489K and pHP51K, respectively. Transformation frequency of pHP51K by electroporation was low. But pHP489K could be effectively transformed into various H. pylori strains. In order to design an intermdiate vehicle vector for gene transfer into H. pylori, pBlueHP489K was prepared by recloning pHP489K DNA into pBluescript and pTZ19R vector. This vector permitted the DNA fragment containing pHP489 sequence, aph3'-Ⅲ, and cloned DNA to be cut and self-ligated in the SacⅠ site after cloning. ureA and ureB gene were inserted into pBlueHP489K, resulting in pBlueHP489K/AB. The DNA fragment containing pHP489, kanamycin resistance gene(aph3'-Ⅲ), and urease structural gene was cut away from pBlueHP489K/AB and self-ligated to generate pBlueHP489K/AB. pBlueHP489K/AB made urease-negative H. pylori strains restore their urease activity. By this experiment, pBlueHP489K was confirmed to be the vehicle system for transferring H. pylori genes.

Isolation and Characterization of Antimicrobial Substance Macrolactin A Produced from Bacillus amyloliquefaciens CHO104 Isolated from Soil

( Seung Je Lee ),( Jeong Yong Cho ),( Jung Il Cho ),( Jae Hak Moon ),( Ki Deok Park ),( Young Ju Lee ),( Keun Hyung Park ) 한국미생물생명공학회 2004 Journal of microbiology and biotechnology Vol.14 No.3

관상동맥내 스텐트 삽입후 발생한 관상동맥류 2례

제인수,허민영,문치숙,이재호,정수룡,조길현,이영민,김대경,김두일,김동수 인제대학교 백병원 2002 仁濟醫學 Vol.23 No.3

Coronary artery aneurysms are detected with increasing frequency because of the routine use of coronary angiography in the diagnosis and management of coronary artery disease. Although some coronary artery aneurysms are congenital or inflammatory, most are atherosclerotic. The prognosis of atherosclerotic coronary artery aneurysm may depend on the morphology of the aneurysm or the presence of coexisting obstructive disease. Coronary artery aneurysms have been also reported to occur after ballon angioplasty, directional athrectomy, laser angioplasty, and stent implantation with a frequency of 2% to 4%. The long-term outcome of post-intervention coronary artery aneurysm is unknown. We report two cases of the development of a coronary aneurysm 6 months after stent implantation as a treatment of severe obsructive coronary artery disease.

西部慶南 先史文化 地表調査 報告(I) : 河東郡 玉宗面

趙榮濟 부산대사학회 1983 역사와 세계 Vol.- No.7

일 장기 수용시설 노인들의 인지기능장애와 우울증상의 유병율

조맹제,함봉진,이부영,김성윤,신영민,이창인 大韓神經精神醫學會 1998 신경정신의학 Vol.37 No.5

목 적 : 본 연구는 수용시설의 노인들에서 인지기능장애와 우울증상의 유병율을 조사하기 위한 것이다. 방 법 : 한국의 한 수용시설인 꽃동네에 거주하는 60세 이상 노인 169명을 대상으로 한국판 Mini-Mental State Examination(MMSE-K). Short Portable Mental State Questionnaire(SPMSQ), 축약판 Geriatric Depression Scale(SGDS). 그리고 the Center for Epidemiologic Studies Depression (CES-D)을 적용하였다. 결 과 : 전체 169명중 153명(남자=72명, 여자=79명, 그리고 누락=2명)이 검사를 완성하였다. 연령의 범위는 60에서 102세였고, 평균연령은 74.17±8.27(남자71.76±8.14. 여자76.14±7.78)세였다. 교육기간의 범위는 무학에서 16년이었고, 평균교육기간은 3.04±3.93(남자 4.13±4.23, 여자 2.01±3.38)년이었다. 인지기능장애의 유병율은 MMSE-K≤23의 기준으로 56.2%, SPMSQ≤6의 기준으로 26.1% 로 평가되었다. 비교적 분명한 인지기능장애의 기준으로 MMSE-K≤19를 적용하였을 경우 유병율은 25.5%로 이는 SPMSQ≤6을 기준으로 하여 얻은 결과와 유사한 수준이었다. 우울증상의 유병율은 SGDS≥10의 기준으로 19%, CES-D≥25의 기준으로 23.5%로 평가되었다. 비교적 가벼운 우울증상까지 선별하기 위하여 SGDS≥8을 기준으로 할 경우 유병율은 39.2%로 이는 CES-D≥20을 기준으로 하여 얻은 결과와 일치하는 수준이었다. MMSE-K와 SPMSQ, SGDS와 CES-D, MMES-K와CES-D, MMES-K와 SGDS, 그리 고·SPMSQ와 SGDS 사이에 의미있는 상관관계가 관찰되었다. 결 론 : 수용시설 노인들에서 인지기능장애와 우울증상의 유병율은 매우 높은 수준이며, 이러한 결과는 수용시설 노인들에 대한 보다 집중적의 의료서비스가 필요함을 시사한다. Objectives : This study was to investigate the prevalence of cognitive impairment and depressive symptomatology among the long-term institutionalized elderly people. Methods : Mini-Mental State Examination-Korean version(MMSE-K), Short Portable Mental State Questionnaire(SPMSQ), Short version of Geriatric Depression Scale(SGDS), and the Center for Epidemiologic Studies Depression(CES-D) were administerd to 169 elderly residents in an institution, 'Got Dong Rae'. Results : One hundred fifty-three(males=72, females=79, and missing=2) completed the entire examination. Age ranged from 60 to 102 years and the mean of age was 74.17±8.27(males 71.76±8.14, females 76.14±7.78) years. Duration of education ranged from 0 to 16 years and the mean of duration of education was 3.04±3.93(males 4.13±4.23, females 2.01±3.38) years. The prevalence of cognitive impairment was estimated as 56.2% by MMSE-K≤23 and 26.1% by SPMSQ≤6. The prevalence of relatively definite cognitive impairment defined by MMSE-K≤19 was 25.5%, which was similar to the result achieved by using SPMSQ≤6. The prevalence of depressive symptomatology was estimated as 19% by SGDS≥10 and 23.5% by CES-D≥25. In the case of using SGDS≥8 as cutoff-point to screen mild as well as severe depressive symptomatology, the prevalence was 39.2%. It was identical to the result achieved by using CES-D≥20. Significant correlations were observed between MMSE-K and SPMSQ, between SGDS and CES-D, between MMSE-K and CES-D, between MMSE-K and SGDS, and between SPMSQ and SGDS. Conclusion : The prevalencies of cognitive impairment and depressive symptomatology were high in institutionalized elderly people. This results suggests that more intensive mental health care is needed for long-term institutionalized elderly people.

玉田 M₃號墳 發掘調査 槪報

崔榮濟,金貞禮,朴升圭 嶺南考古學會 1988 嶺南考古學 Vol.- No.5

高麗初期 鄕吏職의 由來에 對한 小考 : 鄕吏職名의 變化를 中心으로

趙榮濟 부산대사학회 1980 역사와 세계 Vol.- No.4

玉田古墳의 編年硏究

趙榮濟 嶺南考古學會 1996 嶺南考古學 Vol.- No.18

榮山江 河口 댐 建設前後의 海洋環境 比較硏究

曺炷煥,朴榮濟 조선대학교 기초과학연구소 1989 自然科學硏究 Vol.12 No.1

In order to find out the effect of the enclosure at Yeongsan River Estuary on the surrounding aquatic envionments, a study on the physical, chemical and biological changes in this area had been carried out during from February, 1979 to November, 1985. Since November, 1981 when the dam was completed, there have been the considerable changes in the enclosed estuary in salinity, dissolved oxygen density, COD, nutrients, suspended particulate matter(SPM), transparency and biological compositions, with reduced water flow and appearance of immovable water masses. The relationship between the density of SPM and the transparency was calculated as S=86.4982-71.4837T(r=-0.8610) in 1979, but this was changed after the enclosure of the tam to S=22.0384-6.1865(r=-0.7488) in 1984. The fishes collected during the investigation are consisted of 12 species incuding Fugu vermicularis porphyreus, Epinephelus septemfasciatus and Hippocampus coronatus, which are newly found from this area. New establishments of clear water-algal species in this area such as Codium fragile, Hizikia fusiforme, Sargassum fulvellum and Undaria pinnatifida implies that a great change of marine flora have been occurred after the enclosure of the dam. benthic animals are 11 species including Mytilus edulis, Chlamys farreri, Musculus senhausia and Hemicentrotus pulcherrimus, which are newly inhabited in this area. In paricular M. edulis attached abundantly to the shore, and this implies that there was a great deal of ecological change after the enclosure of dam in this area.

인조 합성 테니스 바닥재의 지면 반력 분석

조승제,정미라,서국웅,박승범,윤양진,이훈식,강영택 釜山大學校 附設 體育科學硏究所 1998 體育科學硏究所 論文集 Vol.14 No.-

Some authors suggest that certain types of surfaces are the origin of such injuries. A few years after the first medical concerns about surfaces were voiced, publications of biomechanical measurements apperared, describing accleration, force, and impact measurement on different types of surfaces. In many sport activities, surfaces can be under very high dynamic load. This was the reason for the development of various methods for impact simulation, like the development of various methods for impact simulation, like the artificial athlete. Furthermore, it is important to collect information about the hardness of new and already existing surfaces in sport arenas. The idea on which this measuring system is based Is as follows: The stiffness of the material can be computed from the kinematics measurd at touch down of a rigid body onto a material sample. The results show the following The result for the artifical surfaces(Synpave ace) is surprising. It is known that these surfaces are much harder than synpave rebound classic, synpave spring. This finding suggests that it may be possible that the subjective impression is used as one criterion in the selection of landing(or style) strategies. The number of subjects in this experiment is too small to make statistically significant conclusions. It is shown analytically that when an object a deformable surface, the acceleration it experiences is inversely propotional to its mass. In future, it need to stress that the interaction between shoe and surface is important, and this aspect has now become well accepted. Considering biomechanical aspect in player's injury, it request Korean Standards for synthetic playing surfaces in sport like ASTM(America Society for Testing & Materials) standards of America, DIN 18035 standards of Germany, BSI standards of U.K.