Aerobic dance를 사용한 3개월 간의 『운동+식사 프로그램』이 비만경향 여성의 형태 및 신체조성에 미치는 영향

육조영,김범수,이승훈,전혜령 한국스포츠리서치 2003 한국 스포츠 리서치 Vol.20 No.1

The purpose of this study was to evaluate the effects of a weight-loss program in obese women. Sixty-seven subjects, aged 37.1±8.7 years, joined in the program that comprised diet and exercise prescription. The exercise prescription consisted of a 60-rnin aerobic dance(2d/wk) and self-selected, unsupervised aerobic exercise. The duration of this program was 3months. As a result, body mass and %fat significantly decreased from 61.9±8.4 to 59.1 ±8.3㎏ and from 30.7±7.5 to 26.6±7.0%(p<0.05), respectively. Fat-free mass(FFM) remained essentially unchanged (42.6±4.2 vs. 43.0±3.7㎏). About 10% of all subjects lost FFM by more than 2㎏, and accordingly, their energy yield was not enough to maintain FFM. Among the subjects who successfully reduced excess fat and maintained FFM no change in energy yield and reduction in upper body fat were found. Conversely, the subjects who could not maintain FFM lost lean tissue in lower limbs. It is suggested that our weight-loss program with exercise is useful in improving body composition and diet ingestion, and that particularly aerobic dance is considered available for reducing excess fat.

생쥐 안구에서 산화적 손상에 의한 superoxide dismutases와 metallothionein의 나이에 따른 유도의 차이

이홍영,이태범,안춘산,변익건,최석민,권대승,최철희 朝鮮大學校 附設 醫學硏究所 2003 The Medical Journal of Chosun University Vol.28 No.2

The basal levels and inducibility of superoxide dismutases (SODs; Mn-SOD and Cu/Zn-SOD) and metallothionein (MT) was investigated in the eyes of mice with age. Oxidative stress was induced by paraquat, an intracellular superoxide generator, in 1, 4, 8, and 12 months of age and then mRl\As of SODs and MT in the eyes were determined by RT-PCR assay. The basal level of Mn-SOD mRNA increased from 1 month to 8 months but decreased thereafter. Mn-SOD mRNA was induced by paraquat until 4 month but not after 8 months. On the other hand, the basal level of Cu/Zn-SOD mRNA decreased with age. Cu/Zn-SOD mRNA was not induced by paraquat until 4 months. The basal level of MT mRNA declined with age whereas its inducibility by paraquat was intact at all ages. Taken together, the results indicate that SODs and MT are differentially expressed and induced according to the age. It could be also implicated that failure in induction of Mn-SOD with age may be one of causative factors in the aging process whereas induction of Cu/Zn-SOD and MT may be one of important factors in defense against oxidative stress in the eyes. 서론: 최근 노화는 반응성 산소종에 의한 산화적 손상의 축적결과라는 설이 각광을 받고 있다. 노화과정에서 노화와 관련된 질병을 비교적 잘 나타내는 안구를 선정하고, 정상생쥐의 안구에서의 superoxide dismutases (SODs) 와 metallothionein (MT) 의 함량 뿐 아니라 산화성 스트레스를 유발하는 paraquat에 의한 SODs와 MT의 유도능과 나이와의 상호관계를 조사하여 이들의 노화과정에서의 역할을 규명하고자 하였다. 재료 및 방법 : 본 연구에서 1, 4, 8, 12개월된 정상생쥐의 안구에서 반응성 산소종 생성의 초기에 중요한 방어기전인 SODs mRNA와 hydroxy radical (OH·)에 의한 산화적 손상의 마지막 방어기전인 MT mRNA의 함량과 산화성 스트레스를 유발하는 paraquat에 의한 유도능을 역전사 중합효소 연쇄반응 (RT-PCR) 으로 조사하였다. 결과: 정상생쥐의 안구의 노화과정에서 Mn-SOD와 Cu/Zn-SOD, 그리고 MT mRNA의 항정상태 함량을 조사한 결과, Mn-SOD mRNA는 8개월까지는 증가하다가 12개월에서 감소하였으나, Cu/Zn-SOD mRNA는 나이와 함께 감소하였다. 그러나 MT mRNA 는 8개월까지는 감소하다가 12개월에서 약간 증가하였다. 산화성 스트레스를 유발하는 parraquat에 의하여 Mn-SOD mRNA는 1개월과 4개월에서만 유도되었고, Cu/Zn-SOD mRNA는 8개월과 12개월에서만 유도되었으며, MT mRNA는 관찰한 전 기간에서 유도되었다. 결론: 이상의 결과를 종합하면 SODs와 MT는 안구에서 나이에 따라 서로 다르게 발현되고 유도되는 것으로 생각되며, 노화과정에서 Mn-SOD유도의 이상이 노화의 원인이 되는 인자로 작용하고, 반면에 Cu/Zn-SOD와 MT의 정상적인 유도능이 산화적 스트레스에 대하여 방어역할을 하는 것으로 생각된다. 이와 같이 연령이 증가함에 따라 Mn-SOD, Cu-Zn-SOD, 및 MT의 발현과 유도능의 차이가 있게 되고, 그 결과 반응성 산소종에 의한 산화적 손상의 정도의 차이로 노화의 속도가 조절되지 않나 생각된다.

유사학과 조정 및 교양교과과정개편에 관한 연구

이종범,김규연,전지용,이춘의,홍석주 朝鮮大學校 1995 교과교육연구 Vol.17 No.-

강진만에서 미생물의 년 변화

박종천,오재영,정용욱,주현수,이원교,유춘만,이우범 國立 麗水大學校 환경문제硏究所 2001 環境硏究論文集 Vol.4 No.-

The biological surveys were performed at seven sites in the Gangjin bay from February, 1998 to November, 2001. In order to analysis of environmental variation, numbers of heterotrophic bacteria, general fungus and fecal coliforms were enumerated in each sample. The result showed that variation range of heterotrophic bacteria, general fungus, and fecal coliforms were 2.9 x 10^(3)~9.6 x 10^(3) CFU/ml, 17.2~80.1 CFU/ml, and 6.3~682.5 CFU/100ml for four tears(1988~2001), respectively. Density of heterotrophic bacteria and general fungus were gradually decreased for four tours, while fecal coliforms were suddenly increased. Specially, numbers of fecal colifoms were increased the more 100 times in 2001 than that 1998. We think that it was probably caused a little rainfall in February, 2001. There result suggests that ecosystem of Gangjin bay were mainly regulated by 2~3 factors, which were inflow of fresh water, salinity and variation of water temperature.

불임환자의 임상적 고찰

강길전,김영범 충남대학교 의과대학 지역사회의학연구소 1991 충남의대잡지 Vol.18 No.2

From January 1990 through October 1991, two-handred and twentyone infertile couples were evaluated for their infertility problem. Of these 221 couples, 79 couples completed six fundamental infertility work-ups. The incidence of infertility was 6.6%. Tubal obstruction was the most common cause of infertility, being the causative factor in half of the patients. The distribution of other causative factor, such as male, ovulatory, peritoneal or cervical factor, was fairly uniform in the range of 22.1%-37.5%. Pregnacy rate was 31.6% and patients with ovulatory disorder were readily achieved pregnancy.

보세판매장 구매자의 쇼핑만족도에 미치는 영향요인에 대한 실증연구

김영춘,최해범 한국관세학회 2009 관세학회지 Vol.10 No.2

In order for us to be able to grasp the factors affecting the development of Bonded Sales Shops, we surveyed the degree of satisfaction of the shopping of the natives and the foreigners. The outcome says that there exists the average difference, between the native shoppers and the foreign shoppers, of the degree of satisfaction of shopping. To see the degree of satisfaction by purchased commodities, in case of native shoppers the degree about the "Tobacco" is highest, and, in case of foreign shoppers "Perfume". The degree of satisfaction by the amount of overseas travelling expenses is highest when the amount is less than 500 US Dollar, irrespective of natives and foreigners. The degree of satisfaction by main visiting purposes is highest when in case of natives the purpose is academic, and, in case of foreigners, it is a shopping and sightseeing. The degree of satisfaction by types of visiting is highest when in case of natives the type is an individual sightseeing and in case of foreigners, it is a sightseeing of more than 6 persons of their own which is sponsored by travelling companies. To see the degree in view point of visiting countries, it is highest when in case of natives the visiting country is one of the Americas, and in case of foreigners the visiting country is one of the South-east countries. To see the degree by types of sales shops, it is highest when in case of natives department stores and downtown duty-free shops, in case of foreigners, airport duty-free shops for foreigners and duty-fee shops on the plane. According to the outcome of a regression analysis, the variable that affects the shopping amount of overseas duty-free shops is the amount of overseas shopping expenses, and it is estimated that the shopping amount at overseas duty-free shops is approximately approaching the 56% of the total amount of overseas shopping expenses. And further, we are able to come to the conclusion that the shopping amount at overseas duty-free shops is not affected by such factors as the total overseas travelling expenses, the shopping amount at domestic duty-free shops, the number of times of overseas travelling, and the degree of satisfaction of the shopping of duty-free shops, etc.

동시공학 설계 개념을 이용한 자동차 현가 장치의 최적 설계

김정욱,전용범,이종원 중앙대학교 미래신기술연구소 2002 미래신기술연구소 논문집 Vol.11 No.2

동시동학 설계 방법은 보다 짧은 개발기간과 저렴한 생산비용으로 보다 우수한 품질을 갖는 제품의 개발을 위해 적용될 수 있다. 본 논문에서는 이러한 동시공학 설계개념을 이용하여 자동차 현가 장치의 최적 설계에 대한 연구를 수행하였다. 본 논문에서 제안한 동시공학 설계 개념을 이용한 자동차 현가 장치의 최적 설계 과정은 전처리 설계 단계, 해석 단계, 후처리 단계로 구성된다. 또한 본 논문에서는 현재 독립식 현가 장치 중에서도 전륜 현가 장치로 널리 쓰이고 있는 맥퍼슨 스트럿 현가 장치에 대해 기구학적 민감도 해석과 함계 이를 통한 최적 설계를 수행함으로써 최초 현가장치를 설계 시, 레이아웃(layout) 결정에 유용하게 적용될 수 있는 새로운 접근법을 제시한다. Concurrent engineering design methods can be applied to meet the demand for the quality product with lower production cost and time. In this paper, the optimal design of the automotive suspension system using concurrent engineering design concept is studied. The optimal design process of the automotive suspension system is composed of a pre-processing design stage, and analysis stage and a post-processing stage. In this paper, optimal design with kinematic sensitivity analysis for a McPherson strut suspension system which is widely used for a from wheel suspension is proposed and new approach method which can be applied usefully to determine the layout of suspension system is proposed when a engineer designs the suspension system at the first stage.

자폐장애 환자에서 FMR-1 유전 삼염기 반복의 분자생물학적 분석

곽호순,정철호,전효진,장은진,김희철,김정범,박영남 대한소아청소년정신의학회 2000 소아청소년정신의학 Vol.11 No.1

연구목적 : 자폐장애의 원인을 유전학적으로 규명하려는 연구가 시도되고 있으며, 그 중 fragile-X 증후군과의 연관성에 대한 연구가 활발히 진행되고 있다. fragile-X 염색체(Xq27.3)는 세포유전학적 방법으로 증명할 수 있으나 검사에 많은 제약과 단점이 있으므로, 본 연구에서는 보다 신뢰성이 높은 분자 생물학적 방법으로 FMR-1 유전자내 CGG 삼염기 반복부위를 분석하여 자폐장애와 fragile-X 증후군의 연관성을 규명하고자 하였다. 방법 : 자폐장애 환아(99명)와 정상대조군(8명)을 대상으로 FMR-1 유전자의 CGG 반복배열 부위를 sense와 antisense primer를 이용하여 PCR법으로 분석하엿으며, 동시에 세포유전학적 검사도 시행하였다. PCR 분석에서 CGG 반복수가 50 이상인 경우에 대해서는 StB12.3 혹은 Pfxa3 probe를 이용한 Sourthern blot hybridization으로 확인하였다. 결과 : FMR-1 유전자의 CGG 반복배열에 대한 PCR 분석 결과 CGG 삼염기의 반복배열의 수는 자폐장애 환자군과 정상대조군 사이에 통계적으로 유의한 차이가 없었다(p=0.207). 자폐장애 환자에서 CGG 반복수가 50회 이상인 조기변이(premutation) 환자가 2명 있었으나 Sourthern blot hybridization 결과 완전변이(full mutation)로 판정할 수 있는 경우는 없었다. 세포유전학적 검사에서 환자군 모두에서 정상 핵형을 나타내었으며 fragile-X 염색체는 확인되지 않았다. 결론 : 이상의 결과에서 자페장애 환자가 FMR-1 유전자의 CGG 삼염기 반복부위 이상, 즉 fragile-X 염색체 이상을 동반하지 않았음을 증명할 수 있었다. 이는 fragile-X 증후군을 자�장애의 직접적인 원인이라고 보기에는 어려움이 있음을 시사한다. Objectives : There has been a rapid expansion of studies aimed at elucidating the genetic basis of autistic disorder, especially it's relationship to fragile-X syndrome. The detection of fragile X chromosome(Xq27.3) by cytogenetic analysis has revealed many difficulties in testing. Therefore, to explore the relationship between autistic disorder and fragile X syndrome, this study administered molecular biologic methods which examined an unstable CGG repeat within the fragile X mental retardation-1(FMR-1) gene. Methods : Ninety nine autistic children and eight normal control children were tested. The number of CGG repeats within FMR-1 gene was measured after amplification by PCR, and cytogenetic analysis was also carried out to detect fragile site Xq27.3. Sourthern blot hybridization, using StB12.3 and/or Pfxa3 probe, was done for the patients showing expansion of more than 50 CGG repeats(premutation). Results : All but two autistic patients had no expansion in CGG repeats by PCR and there was no significant statistical difference in number of CGG repeat in comparison with normal control. Two autistic patients, considered as premutation by PCR analysis, had no full mutation or premutation by Southern blot hybridization. All autistic children tested did not have any abnormal karyotype or fragile site Xq27.3. Conclusions : These results suggest that autistic patients may not have abnormality in FMR-1 gene or abnormal expansion in CGG repeat. In conclusion, fragile X syndrome may not be antecedent of autistic disorder. KEY WORDS : Autistic disorder·FMR-1 gene trinucleotide repeats·Molecular biologic analysis.

경북지역의 사과 생산변동과 여타지역과의 생산비교

류진춘,신영범,Ryu, Jin Chun,Shin, Young Bum 경북대학교 농업과학기술연구소 1995 慶北大農學誌 Vol.13 No.-

Apple is most favorable fruit in Korea, and apple farmland has been increased before and after the agreements of Uruguay Round and apple is considered as one of strategic agricultural goods. Especially expansion of apple farmland is concentrated in Kyungpook region because of the suitable climate and its market share is about 70 percents in 1992. But in tree age of apple, although newly and replanting area is increasing, the rate of old orchards is higher than that of other regions. In varieties of apple, it is concentrated in Fuji and is forecasted to increase in the future. The amount of apple production of Kyungpook region is 480 thousand tons, that is concentrated in Wui-sung, Young-ju and An-dong. The quantity of apple production per 10a. is 1,315kg, that is low level compared with that of America and Japan, and the gap of technology among farmers is heavily. The difficulties of apple farming in Kyungpook region are summarized as follows. first, the lack of mechanization and facilities due to the small scale of farmland, second, lack of rural labor force, third, concentration on Fuji apple varieties, fourth, low productivity of apple farming.

[P8-36] Antioxidant and Acetylcholinesterase Inhibition Activity of Mulberry Fruit Extracts

Jae Young Je,Soon-Sil Chun,Rae-Young Kim,Chang-Bum Ahn,Ka-Hwa Lee,Young Ju Lee 한국식품영양과학회 2009 한국식품영양과학회 학술대회발표집 Vol.2009 No.11