Preliminary analysis of geographical distribution based on cold hardiness for Evergestis extimalis (Scopoli) (Lepidoptera: Pyralidae) on Qinghai–Tibet Plateau

You‐Peng Lai,Ke Tao,Taiping Hou 한국곤충학회 2019 Entomological Research Vol.49 No.1

Evergestis extimalis (Scopoli) is a pest insect present in spring rape fields of the Qinghai–Tibet plateau. A survey of its distribution and analysis of its physiological and biochemical variances of its overwintering larvae were conducted in this study. Prior to 2006, Evergestis extimalis Scopli appeared only sporadically at the east agricultural district of Qinghai Province at 2,100 m elevation; after 2006, there have been frequent outbreaks at 2,200 m or so height. The insect's distribution has extended continuously toward higher altitudes yearly, and the scope of its damage reached 2,800 m height in 2010. These changes indicate that the cold hardiness of E. extimalis is on the rise. Physiological and biochemical analyses were performed for the insect's overwintering larvae from November 2011 to March 2012. The supercooling point (SCP) and freezing point (FP) ranged from −6.85°C to −12.49°C and from −6.23°C to −8.17°C, respectively, and both were at their respective lowest points in January 2012; the lowest points of water and fat contents (which did not vary to any extreme degree throughout the test period) were also observed in January 2012. Glycogen content varied from 2.42 mg/g to 4.56 mg/g. Protein content increased gradually at the first two months and reached its peak in January 2012 before dropping slightly. The activity of protective enzymes POD, CAT, and SOD varied with changes in environmental temperature, and each was at its lowest point in January 2012. With the exception of protein and glycerol content, other physiological and biochemical variances were generally parallel with environmental temperature, strongly indicating that E. extimalis has indeed developed cold hardiness.

Prognostic Significance of GSTP1, XRCC1 and XRCC3 Polymorphisms in Non-small Cell Lung Cancer Patients

Ke, Hong-Gang,Li, Jun,Shen, Yi,You, Qing-Sheng,Yan, Yu,Dong, Han-Xuan,Liu, Jun-Hua,Shen, Zhen-Ya Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.9

Aim: Individual differences in chemosensitivity and clinical outcome in non-small cell lung cancer (NSCLC) patients treatment with platinum-based chemotherapy may be due to genetic factors. Our study aimed to investigate the prognostic role of GSTP1, XRCC1 and XRCC3 in NSCLC patients treated with chemotherapy. Methods: A total of 460 cases were consecutively selected from The Affiliated Hospital of Nantong University between Jan. 2003 to Nov. 2006, and all were followed-up until Nov. 2011. Genotyping of GSTP1 Ile105Val, XRCC1 Arg194Trp, XRCC1 Arg399Gln and XRCC3 Thr241Met was conducted by duplex polymerase-chain-reaction with confronting-two-pair primer methods. Results: Patients with GSTP Val/Val exhibited a shorter survival time, and had a 1.89 fold greater risk of death than did those with the IIe/IIe genotype. For XRCC1 Arg194Trp, the variant genotype Trp/Trp was significantly associated with a decreased risk of death from NSCLC when compared with the Arg/Arg. Individuals carrying XRCC1 399Gln/Gln genotype had a longer survival time, with a lowered risk of death from NSCLC. Conclusion: This study indicated that GSTP1 Ile105Val, XRCC1 Arg194Trp and XRCC1Arg399Gln genes have a role in modifying the effect of platinum-based chemotherapy for NSCLC patients in a Chinese population. Our findings provide information for therapeutic decisions for individualized therapy in NSCLC cases.

Biosynthesis of well-defined starch magnetic microbeads

Ke Luo,Ki-Baek Jeong,Sang-Mook You,Young-Rok Kim 한국산업식품공학회 2018 학술대회 및 심포지엄 Vol.2018 No.04

Amylose is carbohydrate polymer defined as a linear natural polysaccharide composed of α(1→4) bound glucose units. Due to its abundance, renewable nature, low cost, and biodegradability, this polymer is regarded as a promising green material for producing crystals and particles of different sizes ranging from the nanometer scale to the micrometer scale. Herein, short amylose chains and dextran-coated iron oxide magnetic nanoparticles (Dex@IONPs) were introduced to fabricate well-dispersed starch magnetic microbeads (SMMBs), which have a well-defined spherical shape and a uniform size of about 1 μm. We found that the aggregation of SMMBs can be mediated by the introduced Dex@IONPs in a concentration-dependent manner, indicating that Dex@MNPs, as the seed crystals, play an important role in self-assembly of SMMBSs. By using streptococcal protein G tagged with maltose binding protein (MBP-SPG), specific antibody against Escherichia coli O157:H7 was successfully immobilized on the surface of SMMBs. The Ab-functionalized SMMBs showed a high capture efficiency (>90%) comparable to the commercial immunomagnetic microparticles regardless of suspending agents (1X PBS and milk). Moreover, SMMBs exhibited excellent recyclability, in which the Ab immobilized on the surface of SMMBs can be refreshed by using the maltose elution buffer along with the unchanged capture efficiency. In addition, SMMBSs were assembled into the linear rod-shape microstructure by the introduced magnetic field during the amylose-mediated precipitation process. The convenient self-assembly of SMMBs with the well-defined size and shape, biocompatibility, tolerance to environmental variances, high magnetic response behavior, and excellent recyclability in the functionalization make these magnetic microparticles promising for many potential applications such as bio-sensing, labeling, and smart delivery of active compounds.

Radiation Measurement Uncertainty for Radiological Evaluation of Simulated Radwaste

Ke Chon Choi,Chang Heon Lee,Se Chul Sohn,Seung Su Shin,Pu reun Kim,Seung Min You 한국방사성폐기물학회 2019 한국방사성폐기물학회 학술논문요약집 Vol.2019 No.10

Molecular Rearrangement of Glucans from Natural Starch To Form Size-Controlled Functional Magnetic Polymer Beads

Luo, Ke,Jeong, Ki-Baek,You, Sang-Mook,Lee, Da-Hee,Kim, Young-Rok American Chemical Society, Books and Journals Divi 2018 Journal of agricultural and food chemistry Vol.66 No.26

<P>Herein, we report a fairly simple and environmentally friendly approach for the fabrication of starch-based magnetic polymer beads (SMPBs) with uniform shape and size through spontaneous rearrangement of short-chain glucan (SCG) produced by enzymatic debranching of waxy maize starch. The paramagnetic materials, dextran-coated iron oxide nanoparticles (Dex@IONPs), were readily incorporated into the starch microstructure and rendered a superparamagnetic property to the SMPBs. The morphology and size of resulting SMPBs turned out to be modulated by Dex@IONPs in a concentration-dependent manner, of which Dex@IONPs was assumed to be acting as a seed inducing the epitaxial crystallization of SCG and further transforming it into homogeneous microparticles. The surface of SMPBs was readily functionalized with an antibody through a one-step reaction using a linker protein. The immuno-SMPBs showed great capture efficiency (>90%) for target bacteria. The colloidal stability and favorable surface environment for biomolecules are believed to be responsible for the high capture efficiency and specificity of the SMPBs. Furthermore, the captured bacteria along with antibody and linker protein were effectively eluted from the surface of SMPBs by adding free maltose, making this new material suitable for various chromatographic applications.</P> [FIG OMISSION]</BR>

Polymorphisms in Genes of the De Novo Lipogenesis Pathway and Overall Survival of Hepatocellular Carcinoma Patients Undergoing Transarterial Chemoembolization

Wu, You-Sheng,Bao, Deng-Ke,Dai, Jing-Yao,Chen, Cheng,Zhang, Hong-Xin,Yang, YeFa,Xing, Jin-Liang,Huang, Xiao-Jun,Wan, Shao-Gui Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.3

Aberrant expression of genes in de novo lipogenesis (DNL) pathway were associated with various cancers, including hepatocellular carcinoma (HCC). Single nucleotide polymorphisms (SNPs) of DNL genes have been reported to be associated with prognosis of some malignancies. However, the effects of SNPs in DNL genes on overall survival of HCC patients receiving transarterial chemoembolization (TACE) treatment are still unknown. In present study, nine SNPs in three genes (ACLY, ACACA and FASN) in DNL pathway were genotyped using the Sequenom iPLEX genotyping system in a hospital-based cohort with 419 HCC patients treated with TACE, and their associations with HCC overall survival were evaluated by Cox proportional hazard regression analysis under three genetic models (additive, dominant and recessive). Although we did not find any significant results in total analysis (all p>0.05), our stratified data showed that SNP rs9912300 in ACLY gene was significantly associated with overall survival of HCC patients with lower AFP level and SNP rs11871275 in ACACA gene was significantly associated with overall survival of HCC patients with higher AFP level. We further identified the significant interactions between AFP level and SNP rs9912300 or rs11871275 in the joint analysis. Conclusively, our data suggest that genetic variations in genes of DNL pathway may be a potential biomarker for predicting clinical outcome of HCC patients treated with TACE.

DEVELOPMENT OF NONLINEAR PHENOMENA IN STANDING WAVE

LIU Ke,MAA Dah-You 한국소음진동공학회 1994 한국소음진동공학회 국제학술대회논문집 Vol.1994 No.3

Experimental and Numerical Simulation of Resin Flowing in VARI Process

Geyi You,Xiaoping Gao,Jiawei Chen,Ke Zhao 한국섬유공학회 2023 Fibers and polymers Vol.24 No.8

In this paper, the flow characteristics of resin in vacuum-assisted forming (VARI) process were studied by experiments and numerical simulation. The position and time of resin flow front were recorded by high-speed camera and timer. The viscosity of resin, porosity and permeability of composites were tested. Based on Darcy’s law, the resin permeability model in composite molding was derived, and the permeability was obtained by nonlinear fitting. The unit cell model of plain fabric was established and the flow characteristics of four kinds of viscosity resins were simulated by finite volume method. The flow profile and filling time were compared between experimental and numerical simulation, and the error between the model and the experiment is less than 12%, which verifies the accuracy of the model and shows that the model can be used to predict the flow behavior of resin with different viscosities in composite molding.

Paper-Based Radial Chromatographic Immunoassay for the Detection of Pathogenic Bacteria in Milk

Luo, Ke,Ryu, Jian,Seol, In-Hye,Jeong, Ki-Baek,You, Sang-Mook,Kim, Young-Rok American Chemical Society 2019 ACS APPLIED MATERIALS & INTERFACES Vol.11 No.50

<P>Here, a paper-based radial flow chromatographic immunoassay (RFCI) employing gold nanoparticles (AuNPs) as chromatic agents was developed for the detection of <I>Escherichia coli</I> O157:H7 in whole milk. A 4-repeated gold-binding peptide-tagged (4GBP) streptococcal protein G (SPG) fusion protein was constructed as a bifunctional linker to immobilize antibodies on the surface of AuNPs with a well-oriented form based on the specific affinity of GBP and SPG to the gold and Fc portion of the antibody, respectively. 4GS@AuNPs prepared with the bifunctional linker protein exhibited excellent colloidal stability even at high salt concentrations of up to 500 mM, which is a critical requirement for its application to a broad range of biological and food samples. The enhanced colloidal stability and excellent binding capability of the immuno-4GS@AuNPs toward target bacteria lowered the detection limit of RFCI for target pathogenic bacteria in whole milk as low as 10<SUP>3</SUP> CFU/mL, which is by an order of magnitude lower than that of conventional immuno-AuNPs prepared with physical adsorption of antibodies. The RFCI pattern could also be converted into a grayscale value by simple image processing for quantitative determination of target pathogenic bacteria. This paper-based detection system would provide an effective means of monitoring the presence of food-borne pathogens in real food samples with naked eyes.</P> [FIG OMISSION]</BR>

The clinical performance of APTIMA human papillomavirus and Hybrid Capture 2 assays in the triage of lesser abnormal cervical cytologies

Yanli Guo,Ke You,Li Geng,Jie Qiao 대한부인종양학회 2014 Journal of Gynecologic Oncology Vol.25 No.4

Objective: This study was performed to evaluate the clinical performance of APTIMA human papillomavirus (AHPV) assay and Hybrid Capture 2 (HC2) assay in screening for cervical disease, especially in women with atypical squamous cell of undetermined significance (ASC-US) and low-grade squamous intraepithelial lesion (LSIL). Methods: A total of 411 women diagnosed with ASC-US or LSIL were referred and further triaged by HC2 test. Prior to colposcopy, liquid-based cytology specimens were collected for the AHPV assay. Sensitivity and specificity were established based on the histological findings of cervical intraepithelial neoplasia (CIN). Results: In all 411 subjects, the positive detection rate of AHPV assay was 70.8% (95% confidence interval [CI], 66.4 to 75.2), which was significantly lower than the positive detection rate of 94.9% obtained using HC2 test (95% CI, 92.3 to 96.8). Only one CIN 3-positive case was detected among the 120 AHPV-negative women, which was then confirmed by Pap smear test to be LSIL. The sensitivities of AHPV and HC2 for CIN 3 were similar (94.1% and 100%, respectively). However, AHPV showed a significantly higher specificity than HC2 test (30.2% and 5.3%, respectively; p<0.001). Conclusion: AHPV assay is effective in identifying CIN 3-positive cases because of its high specificity and lower false-negative rate. The use of AHPV for the triage of ASC-US and LSIL might help to reduce the referral rate of colposcopy during cervical cancer screening.