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        Preliminary analysis of geographical distribution based on cold hardiness for Evergestis extimalis (Scopoli) (Lepidoptera: Pyralidae) on Qinghai–Tibet Plateau

        You‐Peng Lai,Ke Tao,Taiping Hou 한국곤충학회 2019 Entomological Research Vol.49 No.1

        Evergestis extimalis (Scopoli) is a pest insect present in spring rape fields of the Qinghai–Tibet plateau. A survey of its distribution and analysis of its physiological and biochemical variances of its overwintering larvae were conducted in this study. Prior to 2006, Evergestis extimalis Scopli appeared only sporadically at the east agricultural district of Qinghai Province at 2,100 m elevation; after 2006, there have been frequent outbreaks at 2,200 m or so height. The insect's distribution has extended continuously toward higher altitudes yearly, and the scope of its damage reached 2,800 m height in 2010. These changes indicate that the cold hardiness of E. extimalis is on the rise. Physiological and biochemical analyses were performed for the insect's overwintering larvae from November 2011 to March 2012. The supercooling point (SCP) and freezing point (FP) ranged from −6.85°C to −12.49°C and from −6.23°C to −8.17°C, respectively, and both were at their respective lowest points in January 2012; the lowest points of water and fat contents (which did not vary to any extreme degree throughout the test period) were also observed in January 2012. Glycogen content varied from 2.42 mg/g to 4.56 mg/g. Protein content increased gradually at the first two months and reached its peak in January 2012 before dropping slightly. The activity of protective enzymes POD, CAT, and SOD varied with changes in environmental temperature, and each was at its lowest point in January 2012. With the exception of protein and glycerol content, other physiological and biochemical variances were generally parallel with environmental temperature, strongly indicating that E. extimalis has indeed developed cold hardiness.

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        Isolation and Identification of Paracoccus sp. FD3 and Evaluation of Its Formaldehyde Degradation Kinetics

        Haoyu Zhao,Yucong Geng,Jieyu Fan,Ke Tao,Taiping Hou 한국생물공학회 2013 Biotechnology and Bioprocess Engineering Vol.18 No.2

        A formaldehyde-degrading bacterium strain,FD3, was isolated from contaminated soil and identified as Paracoccus sp. based on partial 16S rRNA gene sequence analysis. In batch culture, the bacterium metabolized 5,000and 8,000 mg/L formaldehyde completely within 16 and 18 h, respectively, at 30oC (pH 7.0) with agitation at 150 rpm. The degradation kinetics was found to follow a first-order model at all initial formaldehyde concentrations with regression values greater than 0.99. Formaldehyde degradation rates increased from 532.37 to 2283.04 mg/L/h as the initial concentration of formaldehyde was increased from 1,000 to 8,000 mg/L. The growth of strain FD3 on formaldehyde as a sole carbon and energy source was well described by the Luong model with a maximal specific growth rate of 0.1754/h, a half-saturation constant of 309.02 mg/L, and a maximum substrate concentration of 3875.53 mg/L. Due to its high tolerance and degradation capacity to formaldehyde, Paracoccus sp., FD3 is considered an excellent candidate for use in degrading formaldehyde in wastewaters.

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