Transgenic Mouse Expressing Optical MicroRNA Reporter for Monitoring MicroRNA-124 Action during Development

Choi, Yoori,Hwang, Do won,Kim, Mee Young,Kim, Joo Yeon,Sun, Woong,Lee, Dong Soo Frontiers Media S.A. 2016 Frontiers in molecular neuroscience Vol.9 No.-

<P>MicroRNAs (miRNAs) fine-tune target protein synthesis by suppressing gene expression, temporally changing along development and possibly in pathological conditions. A method to monitor the action of miRNAs <I>in vivo</I> shall help understand their dynamic behavior during development. In this study, we established a transgenic mouse harboring miR-124 responsive element in their luciferase-eGFP reporter transgenes which enabled monitoring the action of miR-124 in the brain and other organs <I>in vivo</I> by the bioluminescence imaging. The mouse model was produced and verified by imaging <I>ex vivo</I> so that luminescence by luciferase shone and then reduced during development with miR-124 expression. Bioluminescence dramatically decreased in the brain between embryonic day 13 and 16 as endogenous miR-124 expression increased, which sustained into adulthood. The inverse relationship of miR-124 expression was observed with luciferase bioluminescence and activity <I>ex vivo</I> as well as <I>in vivo</I>. Taken together, one can use this microRNA-transgenic mouse to investigate the temporal changes of microRNA action <I>in vivo</I> in the brain as well as in other organs.</P>

Development of tau PET Imaging Ligands and their Utility in Preclinical and Clinical Studies

Choi, Yoori,Ha, Seunggyun,Lee, Yun-Sang,Kim, Yun Kyung,Lee, Dong Soo,Kim, Dong Jin The Korea Society of Nuclear Medicine 2018 핵의학 분자영상 Vol.52 No.1

The pathological features of Alzheimer's disease are senile plaques which are aggregates of ${\beta}$-amyloid peptides and neurofibrillary tangles in the brain. Neurofibrillary tangles are aggregates of hyperphosphorylated tau proteins, and these induce various other neurodegenerative diseases, such as progressive supranuclear palsy, corticobasal degeneration, frontotemporal lobar degeneration, frontotemporal dementia and parkinsonism linked to chromosome 17 (FTDP-17), and chronic traumatic encephalopathy. In the case of Alzheimer's disease, the measurement of neurofibrillary tangles associated with cognitive decline is suitable for differential diagnosis, disease progression assessment, and to monitor the effects of therapeutic treatment. This review discusses considerations for the development of tau ligands for imaging and summarizes the results of the first-in-human and preclinical studies of the tau tracers that have been developed thus far. The development of tau ligands for imaging studies will be helpful for differential diagnosis and for the development of therapeutic treatments for tauopathies including Alzheimer's disease.

Minocycline Attenuates Neuronal Cell Death and Improves Cognitive Impairment in Alzheimer's Disease Models

Choi, Yoori,Kim, Hye-Sun,Shin, Ki Young,Kim, Eun-Mee,Kim, Minji,Kim, Hyun-Soo,Park, Cheol Hyoung,Jeong, Yun Ha,Yoo, Jongman,Lee, Jean-Pyo,Chang, Keun-A,Kim, Seonghan,Suh, Yoo-Hun American College of Neuropsychopharmacology 2007 Neuropsychopharmacology Vol.32 No.11

Minocycline is a semi-synthetic tetracycline antibiotic that effectively crosses the blood–brain barrier. Minocycline has been reported to have significant neuroprotective effects in models of cerebral ischemia, traumatic brain injury, amyotrophic lateral sclerosis, and Huntington's and Parkinson's diseases. In this study, we demonstrate that minocycline has neuroprotective effects in in vitro and in vivo Alzheimer's disease models. Minocycline was found to attenuate the increases in the phosphorylation of double-stranded RNA-dependent serine/threonine protein kinase, eukaryotic translation initiation factor-2 α and caspase 12 activation induced by amyloid β peptide<SUB>1–42</SUB> treatment in NGF-differentiated PC 12 cells. In addition, increases in the phosphorylation of eukaryotic translation initiation factor-2 α were attenuated by administration of minocycline in Tg2576 mice, which harbor mutated human APP695 gene including the Swedish double mutation and amyloid β peptide<SUB>1–42</SUB>-infused rats. We found that minocycline administration attenuated deficits in learning and memory in amyloid β peptide<SUB>1–42</SUB>-infused rats. Increased phosphorylated state of eukaryotic translation initiation factor-2 α is observed in Alzheimer's disease patients' brains and may result in impairment of cognitive functions in Alzheimer's disease patients by decreasing the efficacy of de novo protein synthesis required for synaptic plasticity. On the basis of these results, minocycline may prove to be a good candidate as an effective therapeutic agent for Alzheimer's disease.Neuropsychopharmacology (2007) 32, 2393–2404; doi:10.1038/sj.npp.1301377; published online 4 April 2007

Spatiotemporal characterization of glial cell activation in an Alzheimer’s disease model by spatially resolved transcriptomics

Choi Hongyoon,Lee Eun Ji,Shin Jin Seop,Kim Hyun Je,Bae Sungwoo,Choi Yoori,Lee Dong Soo 생화학분자생물학회 2023 Experimental and molecular medicine Vol.55 No.-

The molecular changes that occur with the progression of Alzheimer’s disease (AD) are well known, but an understanding of the spatiotemporal heterogeneity of changes in the brain is lacking. Here, we investigated the spatially resolved transcriptome in a 5XFAD AD model at different ages to understand regional changes at the molecular level. Spatially resolved transcriptomic data were obtained from 5XFAD AD models and age-matched control mice. Differentially expressed genes were identified using spots clustered by anatomical structures. Gene signatures of activation of microglia and astrocytes were calculated and mapped on the spatially resolved transcriptomic data. We identified early alterations in the white matter (WM) of the AD model before the definite accumulation of amyloid plaques in the gray matter (GM). Changes in the early stage of the disease involved primarily glial cell activation in the WM, whereas the changes in the later stage of pathology were prominent in the GM. We confirmed that disease-associated microglia (DAM) and astrocyte (DAA) signatures also showed initial changes in WM and that activation spreads to GM. Trajectory inference using microglial gene sets revealed the subdivision of DAMs with different spatial patterns. Taken together, these results help to understand the spatiotemporal changes associated with reactive glial cells as a major pathophysiological characteristic of AD. The heterogeneous spatial molecular changes apply to identifying diagnostic and therapeutic targets caused by amyloid accumulation in AD.

출생순위에 따른 부모화 경험과 자기분화의 관계

최유리 ( Yoori Choi ),송현주 ( Hyunjoo Song ) 아시아문화학술원 2018 인문사회 21 Vol.9 No.3

본 연구는 중학생과 대학생 집단을 대상으로 출생순위와 자기분화의 관계에서 부모화 경험의 매개효과가 연령별로 어떤 차이를 나타내는지 알아보고자 하였다. 이를 위해 남·여 총 308명을 대상으로 부모화 척도(FRS-Y), 자기분화 척도를 사용하였고, 자료분석은 SPSS 18.0 프로그램을 이용하여 상관분석, 위계적 회귀분석을 통해 검증하였다. 연구 결과, 중학생 집단에서는 부모화 경험이 출생순위와 자기분화간의 관계를 부분 매개한 반면, 대학생 집단에서는 유의미한 매개효과를 보이지 않았는데, 어린 연령의 자녀일수록 출생순위라는 가족변인에 더 큰 영향을 받을 뿐만 아니라, 부모화 경험을 높게 지각하며 간접적으로 자기분화 수준을 감소시키는 것으로 나타났다. 본 연구는 전기 청소년에 해당하는 중학생과 후기 청소년에 해당하는 대학생 집단을 연구대상으로 선정함으로써 출생순위가 부모화와 자기분화에 미치는 영향이 연령집단에 따라 차이가 있다는 것을 경험적으로 증명하였다는 점에서 그 시사점이 있다고 볼 수 있다. This study examined how the mediating effects of the parentification experience in the relationship between parentification and differentiation of self change according to age. For doing this, we used the self report measure of parentification (FRS-Y) and differentiation of self targeting at a total of 308 males and females students. For data analysis, we verified through correlation analysis, and hierarchical regression analysis using SPSS 18.0 program. In the middle school student group, the experience of parentification partially mediated the relationship between the birth order and differentiation of self while in the college student group, the experience of parentification did not show signification mediation effects on the relationship between the birth order and differentiation of self. This study bears its significance in that it empirically verified the difference in the effects of the birth order on parentification and differentiation of self according to age groups by targeting pre-adolescence middle school student groups and post-adolescence college student groups.

음식 군집분석을 통한 개인맞춤형 식이 코칭 기법

오유리 ( Yoori Oh ),최지은 ( Jieun Choi ),김윤희 ( Yoonhee Kim ) 한국정보처리학회 2016 정보처리학회논문지. 소프트웨어 및 데이터 공학 Vol.5 No.6

현대인의 건강관리에 대한 관심이 증가하고 다양한 만성질환을 야기하는 식습관에 대한 중요성이 강조되고 있는 상황이다. 이에 따라 여러 가지 모바일 및 웹시스템을 이용한 식단 관리 방법이 등장하고 있지만 이는 실제로 적용하기 어렵고 사용자의 상황을 반영하는 맞춤형 정보를 제공하지 않는다. 따라서 개인의 신체정보 및 상황을 반영하고 음식을 분석하여 실질적으로 사용자가 섭취 가능한 맞춤형 식단관리 및 추천 방법이 필요하다. 본 논문에서는 자기조직화지도를 이용하여 음식을 분석하고 이를 군집화하여 음식에 대한 데이터를 준비한다. 그리고 사용자의 신체정보 및 상황을 고려한 개인 맞춤형 기준을 반영하여 섭취하고 싶은 음식에 대한 피드백 및 대체음식 추천방법을 제안한다. 또한 실험을 통하여 일반적인 방법을 이용한 추천된 음식결과와 비교하여 제안된 방법의 입력 음식과 추천 음식의 거리가 짧다는 것을 통하여 영양적으로 유사한 음식이 추천됨을 증명하였다. In recent times, as most people develop keen interest in health management, the importance of cultivating dietary habits to prevent various chronic diseases is emphasized. Subsequently, dietary management systems using a variety of mobile and web application interfaces have emerged. However, these systems are difficult to apply in real world and also do not provide personalized information reflective of the user``s situation. Hence it is necessary to develop a personalized dietary management and recommendation method that considers user``s body state information, food analysis and other essential statistics. In this paper, we analyze nutrition using self-organizing map (SOM) and prepare data about nutrition using clustering. We provide a substitute food recommendation method and also give feedback about the food that user wants to eat based on personalized criteria. The experiment results show that the distance between input food and recommended food of the proposed method is short compared to the recommended food results using general methods and proved that nutritional similar food is recommended.

공유가치창출(CSV)전략이 반영된 시각장애인을 위한 제품 디자인 사례분석

최보윤(Choi, Boyun),구유리(Koo, Yoori) 한국디자인학회 2017 한국디자인학회 학술발표대회 논문집 Vol.2016 No.10

고객 경험 측정 전략에 대한 연구 : 서비스디자인 관점에서 측정 방법의 비교분석을 통해서

최선아(Choi SunAh),구유리(Koo Yoori) 한국디자인학회 2019 한국디자인학회 학술발표대회 논문집 Vol.2019 No.11

전자감독 특별사법경찰제도 시행 및 개선방안에 관한 연구

최지선(Jisun Choi),성유리(Yoori Seong),김미진(Mijin Kim),김면기(Myeonki Kim) 한국형사법무정책연구원 2021 형사정책연구원 연구총서 Vol.- No.-

Neovascularization in Outer Membrane of Chronic Subdural Hematoma : A Rationale for Middle Meningeal Artery Embolization

Hyun Kim,Yoori Choi,Youngsun Lee,Jae-Kyung Won,Sung Ho Lee,Minseok Suh,Dong Soo Lee,Hyun-Seung Kang,Won-Sang Cho,Gi Jeong Cheon 대한신경외과학회 2024 Journal of Korean neurosurgical society Vol.67 No.2

Objective : Chronic subdural hematomas (cSDHs) are generally known to result from traumatic tears of bridging veins. However, the causes of repeat spontaneous cSDHs are still unclear. We investigated the changes in vasculature in the human dura mater and outer membrane (OM) of cSDHs to elucidate the cause of their spontaneous repetition. Methods : The dura mater was obtained from a normal control participant and a patient with repeat spontaneous cSDHs. The pathological samples from the patient included the dura mater and OM tightly adhered to the inner dura. The samples were analyzed with a particular focus on blood and lymphatic vessels by immunohistochemistry, 3-dimensional imaging using a transparent tissue clearing technique, and electron microscopy. Results : The dural border cell (DBC) layer of the dura mater and OM were histologically indistinguishable. There were 5.9 times more blood vessels per unit volume of tissue in the DBC layer and OM in the patient than in the normal control. The DBC layer and OM contained pathological sinusoidal capillaries not observed in the normal tissue; these capillaries were connected to the middle meningeal arteries via penetrating arteries. In addition, marked lymphangiogenesis in the periosteal and meningeal layers was observed in the patient with cSDHs. Conclusion : Neovascularization in the OM seemed to originate from the DBC layer; this is a potential cause of repeat spontaneous cSDHs. Embolization of the meningeal arteries to interrupt the blood supply to pathological capillaries via penetrating arteries may be an effective treatment option.