Multimorbidity adjusted years lost to disability rates calculated through Monte-Carlo simulation in Korea

Yoonhee Shin,Eun Jeong Choi,Bomi Park,Hye Ah Lee,Eun-Kyung Lee,Hyesook Park 한국역학회 2022 Epidemiology and Health Vol.44 No.-

OBJECTIVES To efficiently utilize limited health and medical resources, it is necessary to accurately measure the level of health, which requires estimating the multimorbidity-corrected burden of disease. METHODS This study used 2015 and 2016 data from the National Health Insurance Service, and employed the list of diseases defined in a Korean study of the burden of disease, the criteria for prevalence, and the “cause–sequelae–health state” disease system. When calculating the years lost to disability (YLD), multimorbidity was corrected using Monte-Carlo simulation. RESULTS Correcting for multimorbidity changed YLD at all ages in Korea by -1.2% (95% confidence interval [CI], -24.1 to 3.6) in males and -12.4% (95% CI, -23.0 to 0.3) in females in 2015, and by -10.8% (95% CI, -24.1 to 4.6) in males and -11.1% (95% CI, -22.8 to 1.7) in females in 2016. The YLD rate for non-communicable diseases in males decreased more than that of other disease groups in both years, by -11.8% (95% CI, -19.5 to 3.6) and -11.5% (95% CI, -19.3 to -3.0), respectively. The overall YLD rate changed by -1.3% in the 5-year to 9-year age group, and the magnitude of this change remained similar until the 10-19-year age group, gradually decreased after 20 years of age, and steeply increased to more than 10% in those aged 60 and older. CONCLUSIONS Calculations of YLD should adjust for multimorbidity, as the disease burden can otherwise be overestimated for the elderly, who tend to exhibit a high prevalence of multimorbidity.

Mycorrhizal Fungal Diversity Associated with Six Understudied Ectomycorrhizal Trees in the Republic of Korea

Park Ki Hyeong,Oh Seung-Yoon,Cho Yoonhee,Seo Chang Wan,Kim Ji Seon,Yoo Shinnam,Lim Jisun,Kim Chang Sun,Lim Young Woon 한국미생물학회 2023 The journal of microbiology Vol.61 No.8

Mycorrhizal fungi are key components of forest ecosystems and play essential roles in host health. The host specificity of mycorrhizal fungi is variable and the mycorrhizal fungi composition for the dominant tree species is largely known but remains unknown for the less common tree species. In this study, we collected soil samples from the roots of six understudied ectomycorrhizal tree species from a preserved natural park in the Republic of Korea over four seasons to investigate the host specificity of mycorrhizal fungi in multiple tree species, considering the abiotic factors. We evaluated the mycorrhizal fungal composition in each tree species using a metabarcoding approach. Our results revealed that each host tree species harbored unique mycorrhizal communities, despite close localization. Most mycorrhizal taxa belonged to ectomycorrhizal fungi, but a small proportion of ericoid mycorrhizal fungi and arbuscular mycorrhizal fungi were also detected. While common mycorrhizal fungi were shared between the plant species at the genus or higher taxonomic level, we found high host specificity at the species/OTU (operational taxonomic unit) level. Moreover, the effects of the seasons and soil properties on the mycorrhizal communities differed by tree species. Our results indicate that mycorrhizal fungi feature host-specificity at lower taxonomic levels.

Investigation of the Fungal Diversity of the Federated States of Micronesia and the Construction of an Updated Fungal Inventory

Park, Myung Soo,Yoo, Shinnam,Cho, Yoonhee,Park, Ki Hyeong,Kim, Nam Kyu,Lee, Hyi-Seung,Lim, Young Woon The Korean Society of Mycology 2021 韓國菌學會誌 Vol.49 No.6

The Federated States of Micronesia (FSM) is an island country in the western Pacific and is a known biodiversity hotspot. However, a relatively small number of fungi (236 species) have been reported till July 2021. Since fungi play major ecological roles in ecosystems, we investigated the fungal diversity of FSM from various sources over 2016 and 2017 and constructed a local fungal inventory, which also included the previously reported species. Fruiting bodies were collected from various host trees and fungal strains were isolated from marine and terrestrial environments. A total of 99 species, of which 78 were newly reported in the FSM, were identified at the species level using a combination of molecular and morphological approaches. Many fungal species were specific to the environment, host, or source. Upon construction of the fungal inventory, 314 species were confirmed to reside in the FSM. This inventory will serve as an important basis for monitoring fungal diversity and identifying novel biological resources in FSM.

Well-being Index Scores and Subjective Health Status of Korean Healthcare Workers

Yoonhee Shin,Bohyun Park,Nam-eun Kim,Eunjeong Choi,Minsu Ock,Sun Ha Jee,Sue K. Park,Hyeong Sik Ahn,Hyesook Park 대한예방의학회 2022 예방의학회지 Vol.55 No.3

Objectives: The aim of this study was to evaluate the subjective level of health-related quality of life of Korean healthcare workers using various quality-of-life instruments. Methods: This study included 992 participants, who were doctors and nurses. A survey was conducted between November 28 and December 4, 2019. Data from 954 participants divided into 3 groups (physicians, residents, and nurses) were analyzed. Four measurement tools (29 questions) were used in the survey to evaluate subjective health status and well-being. Results: In the Mayo Well-being Index, burnout during work (88.5%) and emotional difficulties caused by work (84.0%) were frequently cited by the respondents. Regarding questions on burnout and emotional difficulties, residents and nurses had the highest scores (91.0 and 89.6%, respectively). Emotional problems, such as anxiety, depression, and irritability, accounted for a high percentage (73.1%) of the total, while 82.2% of respondents reported that their work schedules interfered with their leisure and family time. There was no significant difference among the groups in subjective health status. However, 10.1% of the residents experienced very low quality of life, which was a higher proportion than that of physicians (2.7%) and nurses (5.2%). Conclusions: The level of well-being that Korean medical workers experienced in relation to work was lower than the results of the United States healthcare workers surveyed using the same tool. This study was unique in that it conducted a subjective quality-of-life survey on Korean healthcare workers.

Genome wide analysis of rice blast pathogen, Magnaporthe oryzae, in Korea

Yoonhee KIM,Hyejeong KIM,Jinsoo KIM,Miyeon JEONG,Soyoung PARK,Suyoung KIM,Jongsun Park,Soonok KIM,Yong-Hwan LEE,Seong Sook HAN,Jae Hwan ROH,Woobong CHOI 한국작물학회 2007 한국작물학회 학술발표대회 논문집 Vol.2007 No.11

Magnaporthe oryzae is a causal pathogen of the rice blast, the most destructive disease of rice worldwide. This ascomycete fungus has been intensively studied as a model organism in plant-pathogen interactions. Recently, the genome sequence of M. oryzae 70-15 was published by International Rice Blast Consortium. This led us to investigate comparative and functional analysis of this pathogen at genome-wide level. As a first step, sequencing project of M. oryzae Korean strain KJ201 was launched to face practical interests on diversity of plant pathogens. We constructed a fosmid library with a copy number controllable pCC1 vector. Over 13,000 end reads from 6637 fosmid clones were generated and anchored on genome sequence of the strain 70-15. Currently, 9,793 end reads, in which 2.684, clones with both ends and 1,757 clones with on end matched, are aligned to the sequence of strain 70-15. In the next step, shotgun sequencing of fosmid clones was conducted for fine scale comparative analysis of chromosome 7 that is well defined in genome sequencing of 70-15. Totally 119 fosmid clones were pooled in region and analyzed. For reasonable intra-species comparison in genome of M. oryzae, two strains, KJ201 and Guy11, were chosen for whole genome shotgun sequencing analysis with GS454 FLX system. About 8X genome coverage sequences were obtained for each strain and being merged for comparative analysis. Genome information of other strains is being also available from China and Japanese research groups and could be joined together for extended comparative analysis and the results give more detail in genome diversity in species level.

황지천 만곡부 외측호안에 설치된 콘크리트 돌출줄눈의 파손특성

박상덕(Park, Sang Deog),신승숙(Shin, Seung Sook),황윤희(Hwang, Yoonhee),케속안(Khe, Sokan),전우성(Jeon, Woo Sung),박호국(Park, Ho Kook) 대한토목학회 2018 대한토목학회 학술대회 Vol.2018 No.10

커리어코칭 전개 과정에 대한 연구

박윤희(Yoonhee Park),기영화(Youngwha Kee) 중앙대학교 한국인적자원개발전략연구소 2010 역량개발학습연구 Vol.5 No.2

본 연구는 향후 확산이 기대되는 커리어코칭을 중심으로 현장에서 성공적으로 진행되었던 커리어코칭 전개 과정에 대해 살펴보는 것을 목적으로 진행하였다. 연구 참여자로 실제 커리어코칭을 진행했던 코치 6명과 그 상대방인 피코치 6명, 총 12명을 선정하였고 연구방법은 질적 연구 중 근거이론 방법을 적용하였다. 연구 결과를 통해 커리어코칭 전개 과정은 '도움의 필요성 인식' 단계, '하나되기 시작' 단계, '삶의 반추를 통한 자기인식' 단계, '자기주도적 커리어 정립' 단계, '상호 협력적 성장' 단계 그리고 '커리어코칭의 일상화' 단계로 이루어짐을 확인할 수 있었다. 이러한 커리어코칭 전개 과정을 통해 커리어코칭은 기존의 연구자들이 언급한 '상호작용 과정', '돕는 것' 그 이상의 하나되기를 요구하는 과정이라는 것을 알 수 있었다. 커리어코칭 전개 과정에서 코치와 피코치는 자기개발의 필요성을 인식하게 되고 커리어코칭 종료 후에도 자기개발과 학습을 통한 성장을 계속하였다. 따라서 커리어코칭은 한 번의 과정으로 끝나기보다는 개인이 일과 삶 사이에 균형을 이루고 지속적 성장이 가능하도록 평생에 걸쳐 반복적으로 진행될 수 있는 개인의 성장과정이라고 할 수 있을 것이다. As career coaching will be prevalent in the future, this study examined the actual process of career coaching. Grounded theory method was used to conduct the study and twelve participants (six coaches and six coachees) were selected. The study identified the process of career coaching: 'understanding the need for help' stage; 'beginning of being oneness' stage; 'self-awareness through reflection on one's own life' stage; 'establishment of self-directed career' stage, 'cooperative growth' stage, and; 'routinization of career coaching' stage. The result of the study indicates that 'interaction between the coach and the coachees' or 'giving support' are not enough in the career coaching process. It requires for the coahces and the coachees 'to become as one'. The coaches and coachees are aware of the need of self-development in the career coaching process and maintain the growth through continuous self-development and learning after the career coaching process. Although the career coaching process can be completed for single process, it can be repeated as to be a continuing personal growth process balancing between work and life.

Dipeptide-functionalized polyamidoamine dendrimer-mediated apoptin gene delivery facilitates apoptosis of human primary glioma cells

Bae, Yoonhee,Green, Eric S.,Kim, Goo-Young,Song, Su Jeong,Mun, Ji Young,Lee, Sunray,Park, Jong-Il,Park, Jong-sang,Ko, Kyung Soo,Han, Jin,Choi, Joon Sig Elsevier 2016 International journal of pharmaceutics Vol.515 No.1

<P><B>Abstract</B></P> <P>Glioblastoma multiform (GBM) is the most frequent and aggressive form of brain tumors in adults. However, the development of more efficient and safe nonviral vector gene therapy represents a promising therapeutic approach, using a tumor-specific killer gene, named apoptin. In this study, we describe the efficacy of non-viral gene delivery vectors, the amino acid-conjugated PAMAM derivatives (PAMAM-H-R and PAMAM-H-K) in delivering a therapeutic gene, displaying affinity toward human primary glioma cells (GBL-14 cells) and dermal fibroblasts. We analyzed transfection efficiency, using luciferase (Luci) and a pDNA encoding for enhanced fluorescent protein (EGFP), and cytotoxicity in both cells. The results show that transfection efficiency of PAMAM-H-R improved compared to native PAMAM dendrimer, but cytotoxicity of PAMAM-H-R and PAMAM-H-K were very low. We treated both cells with a polyplex formation of PAMAM-H-R or PAMAM-H-K/apoptin, and analyzed their cellular uptake and localization by flow cytometry and confocal microscopy. Furthermore, we analyzed the endosomal escape effect using TEM images, and found that PAMAM-H-R showed very fast escape from endosome to the cytosol. Caspase 3 activity assay, cell cycle distribution, and JC-1 analysis showed apoptosis induced by apoptin in GBL-14 cells. This indicates that PAMAM-H-R can be a potential nonviral vector gene delivery carrier for brain tumor therapy. The present study demonstrates that PAMAM-H-R/apoptin gene polyplex can be used as an effective therapeutic candidate for GBM due to its selective induction of apoptosis in primary glioma cells as a potential nonviral gene delivery carrier for brain tumor therapy.</P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

특성화고등학교 디지털분야 인력양성을 위한 웹프로그래머 직무분석 및 교육훈련과정 개발

박윤희(Yoonhee Park),남양희(Yanghee Nam),이재영(Jae Young Lee),장운민(Wunmin Jang),김지수(Jisoo Kim),송영주(Youngjoo Song) 한국직업교육학회 2023 職業 敎育 硏究 Vol.42 No.4

이 연구는 미래유망분야 고졸인력 양성사업에 참여하는 특성화고의 디지털분야 인력양성을 위하여 웹프로그래머에 관한 직무분석을 실시하고, 이를 바탕으로 교육훈련과정을 개발하는데 목적이 있다. 이를 위해 DACUM 기법을 활용하여 웹프로그래머 직무분석을 실시한 후, 초안에 대해 전문가 집단의 타당성 검토를 거쳐 직무분석 내용을 5개의 책무(Duty)와 16개의 작업(Task)으로 도출하였다. 도출된 DACUM 결과에 기초하여 산업체 전문가와 교육훈련과정을 개발하고, 연계 훈련기관 담당자의 검토를 거쳐 최종적으로 연계 훈련기관에서 활용할 교육훈련과정을 확정하였다. 이와 함께 특성화고 학생들이 웹프로그래머 교육훈련과정을 이수하여 웹프로그래머 분야 입직 이후 지속적인 경력개발이 가능하도록 산업체 전문가들과 논의를 거쳐 경력개발 경로를 제시하였다. 본 연구의 결과를 바탕으로 특성화고와 민간훈련기관의 공동육성체계를 통하여 디지털신기술 분야로 개편한 특성화고 학과의 안정적인 교육훈련체계 수립을 지원하는 데 기여할 수 있을 것으로 기대된다. 나아가 디지털신기술 분야의 고졸자들이 초급인력으로 출발하여 고급인력으로 성장할 수 있도록 경력개발을 제시함으로써 특성화고 학생들의 인력양성에 기여할 수 있을 것으로 예상된다. 마지막으로 디지털신기술 분야의 웹프로그래머 인력양성을 위하여 연계 훈련기관의 교육훈련과정 운영과 학생들의 경력개발 지원을 위한 제언을 제시하였다. The purpose of this study was to conduct a job analysis of web programmers for specialized vocational high school students and develop training courses to develop workforce in a digital field. To achieve this research goal, a job analysis was performed using a DACUM technique and resulted in five duties and sixteen tasks from the draft being analyzed by a group of experts for validation. The draft analysis was reviewed and validated by a group of experts. Based on the DACUM results, we developed training courses with SMEs(subject matter experts), and the training course was finally confirmed by experts at the affiliated training institution. In addition, the career path was developed so that specialized vocational high school students could continue to develop their careers within the web programming field after the training. Based on the results of this study, several suggestions are presented for the course implementation of web programmer at the affiliated training institutions and students’ future career path. Moreover, the results of the study are expected to contribute to the cultivation of a skilled workforce among specialized vocational high school students.

이케노타이가(池大雅, 1723-1776)의 문인화에 대한 이해와 실제

박윤희(Park Yoonhee) 한국미술사학회 2007 美術史學硏究 Vol.255 No.255

본 논고는 일본의 南畵家인 이케노 타이가(池大雅, 1723-1776)의 작품세계를 통해 일본 근세 화단에 文人畵의 개념과 양식이 수용되는 과정에 대해 고찰하였다. 南畵란 중국에서 전래된 明ㆍ淸 회화에 자극받아 유행한 화풍을 일컫는다. 江戶시대(1600-1868) 중기에 활약한 町人 출신의 화가 이케노 타이가는 평생 문인화 그리기를 지향했다. 그러나 18세기 중기는 일본 문인화의 발전 단계에 있어 전반기에 속한다. 文化ㆍ文政期(1804-1830)에 이르러 비로소 남화 이론을 정립한 화론서와 화보 등이 쏟아져 나왔기 때문이다. 따라서 그의 화풍 정립 과정에 대해 살펴보는 작업은 일본 남화사의 전개과정을 이해하는 데 도움이 된다. 타이가는 청소년기에 畵師의 길로 들어서면서 대중에게 사랑받는 町繪 대신에 중국에서 들여온 화보를 보고 문인화풍의 그림을 그렸다. ‘文人ㆍ士大夫’ 계층이 존재하지 않았던 일본 전통 사회에 중국적 취미를 즐기는 지식인들이 점차 늘어났기 때문이다. 야나기사와 키엔(柳澤淇園), 기온낭카이(祖園南海) 등 선배 화가들과 여러 한학파 지식인들과의 교유는 문인화가로서 자긍심을 더욱 높여주었다. 중국에서 전래된 화보와 회적은 문인화의 이론과 기법을 익히는 데 큰 역할을 했다. 『唐詩畵譜』는 문인화의 詩ㆍ畵 일치의 개념을 이해하는 데 도움이 되었다. 역대 화가들의 화풍으로 그린 『顧民畵譜』는 大家들의 화법을 익히고, 倣作 개념을 이해하는 계기가 되었다. 특히 『芥子園畵傳』은 문인화의 기본 화법을 익히는 교과서 역할을 했다. 20-30대 작품에 나타난 여러 가지 화풍에는 전래 화적의 영향이 컸다. 타이가는 다양한 화풍의 중국화를 기무라 겐카도(木村??堂)를 비롯한 지인들을 통해 접할 수 있었다. 그가 자주 사용한 點描法은 蘇州畵派의 화풍과 밀접한 관계가 있다. 그러나 무엇보다도 타이가의 업적은 화법의 꾸준한 연마를 통해 독자적인 양식을 이루어 냈다는 점에 있다. 기존의 남화가들이 주로 화보의 도안이나 중국 화적들을 臨摹하는 수준에 머물렀던 반면, 타이가는 화법의 기본을 익히고 이를 재해석하는 데 주력했다. 문인화 이론을 정립한 明末의 董其昌은 古法의 학습을 통해 궁극적으로는 독자적인 양식을 구축해야 한다고 주장했다. 바로 이러한 이론은 타이가에게 받아들여졌다. 倣作이라는 복고적인 기법을 통해 南宗畵는 물론 일본 전통 회화의 이미지까지 소화하여 창의성을 더했다는 점에서 타이가 회화의 의의를 찾을 수 있다. This article investigates the process of the reception of the concept and style of literati painting in modem japanese artistic circles with a particular focus on works by Ikeno Taiga (1723-1776). Nanga refers to the style of painting produced under the stimulus of Ming and Qing paintings transmitted from China. Ikeno Taiga, a j?nin painter active in mid-Edo period (1600-1868), pursued literati painting throughout his life. The middle of the 18th century belongs to an early stage in the development of Japanese literati painting, since books on the theory of nanga and painting manuals were not published in large numbers until the period between 1804 and 1830. An examination of the formation of his painting style helps understanding the developmental process of the japanese nanga. As he entered the field of painting in his adolescence, Ikeno Taiga did not engage in the contemporary urban painting but mainly worked in literati style painting imitating painting manuals transmitted from China. In the traditional japanese society where the literati class did not exist, the number of intellectuals who developed taste for Chinese literati culture increased gradually. His acquaintance with senior painters such as Yanagisawa Kien and Gion Nankai as well as a number of intellectuals versed in Chinese writings stimulated his pride as a literati painter. Paintings manuals and works transmitted from China played an important role in Ikeno Taiga's learning of the literati theory and techniques. The Tangshibuapu was particularly helpful in understanding the accord of poetry, painting and calligraphy. The Gushihuapu helped him learn the methods of great masters and comprehend the idea of copying. The Jieziyuan huazbuan was a textbook for him in practicing in the basic techniques of literati painting. Various painting styles executed in the works of his 20s and 30s reflect enormous influence from actual Chinese paintings transmitted to Japan. Ikeno Taiga had access to Chinese paintings of diverse styles in association with such acquaintances as Kimura Kengado. Pointillism frequendy used by Ikeno Taiga was derived from the style of the Suzhou school. However, Taiga's achievement is the establishment of his original style through continuous practice based on painting manuals. While earlier nanga painters were content with simply copying Chinese works, he devoted himself to reinterpreting the Chinese style in his own way.