Relaxin is up‐regulated in the rat ovary by orthodontic tooth movement

Yang, So‐,Young,Ko, Hyun‐,Mi,Kang, Jee‐,Hae,Moon, Yeon‐,Hee,Yoo, Hong‐,Il,Jung, Na‐,Ri,Kim, Min‐,Seok,Cho, Jin‐,Hyung,Oh, Won‐,Mann,Kim, Sunȁ Blackwell Publishing Ltd 2011 European journal of oral sciences Vol.119 No.2

<P> <I>Yang S‐Y, Ko H‐M, Kang J‐H, Moon Y‐H, Yoo H‐I, Jung N‐R, Kim M‐S, Cho J‐H, Oh W‐M, Kim S‐H. Relaxin is up‐regulated in the rat ovary by orthodontic tooth movement.</I> 
 <I>Eur J Oral Sci 2011; 119: 115–120. © 2011 Eur J Oral Sci</I> </P><P>Relaxin (Rln) is an ovarian hormone that stimulates osteoclastic and osteoblastic activities and connective tissue turnover. To investigate the expression of Rln during orthodontic tooth movement, rats were implanted with orthodontic appliances that connected a spring from the upper incisors to the first molar with a 70 cN force. Rats in each group were killed 6, 48, and 144 h after activating the appliance, and the levels of <I>Rln1</I> and <I>Rln3</I> expression in the ovary were determined by real‐time RT‐PCR, northern blots, western blots, and immunofluorescence analyses. The amount of tooth movement induced by the orthodontic force increased in a time‐dependent manner. The levels of <I>Rln1</I> mRNA increased by 12‐, 41‐, and 263‐fold at 6, 48, and 144 h, respectively, after orthodontic tooth movement. The time‐dependent increase in the concentration of Rln 1 protein in the ovary was also confirmed by western blotting. Rln 1 was localized in the granulosa cells of the ovarian follicles, and the immunoreactivity against Rln 1 was increased by the movement. In contrast, the concentration of Rln 3 was below the level of detection. The results of this study suggest that local changes in periodontal tissues induced by orthodontic tooth movement may affect <I>Rln1</I> expression in the ovary. However, further studies are needed to decipher the mechanisms involved and the possible contribution of the increased level of expression of Rln 1 to the tooth movement.</P>

Isolation and identification of mammalian orthoreovirus type 3 from a Korean roe deer (Capreolus pygargus)

Yang, Dong-Kun,An, Sungjun,Park, Yeseul,Yoo, Jae Young,Park, Yu-Ri,Park, Jungwon,Kim, Jong-Taek,Ahn, Sangjin,Hyun, Bang-Hun The Korean Society of Veterinary Science 2021 大韓獸醫學會誌 Vol.61 No.2

Mammalian reovirus (MRV) causes respiratory and intestinal disease in mammals. Although MRV isolates have been reported to circulate in several animals, there are no reports on Korean MRV isolates from wildlife. We investigated the biological and molecular characteristics of Korean MRV isolates based on the nucleotide sequence of the segment 1 gene. In total, 144 swabs from wild animals were prepared for virus isolation. Based on virus isolation with specific cytopathic effects, indirect fluorescence assays, electron microscopy, and reverse transcription-polymerase chain reaction, only one isolate was confirmed to be MRV from a Korean roe deer (Capreolus pygargus). The isolate exhibited a hemagglutination activity level of 16 units with pig erythrocytes and had a maximum viral titer of 10^5.7 50% tissue culture infectious dose (TCID₅₀)/mL in Vero cells at 5 days after inoculation. The nucleotide and amino-acid sequences of the partial segment S1 of the MReo2045 isolate were determined and compared with those of other MRV strains. The MReo2045 isolate had nucleotide sequences similar to MRV-3 and was most similar (96.1%) to the T3/Bat/Germany/342/08 strain, which was isolated in Germany in 2008. The MReo2045 isolate will be useful as an antigen for sero-epidemiological studies and developing diagnostic tools.

Biological and molecular characterization of feline caliciviruses isolated from cats in South Korea

Yang, Dong-Kun,Park, Yu-Ri,Yoo, Jae Young,Choi, Sung-Suk,Park, Yeseul,An, Sungjun,Park, Jungwon,Kim, Heui-Jin,Kim, Jongho,Kim, Ha-Hyun,Hyun, Bang-Hun The Korean Society of Veterinary Science 2020 大韓獸醫學會誌 Vol.60 No.4

Feline calicivirus (FCV) infection results in a common upper respiratory disease associated with oral ulceration in cats. Although FCV infection has been reported in cats worldwide, the biologic and genetic features of South Korean FCV are unclear. We aimed to investigate the biological and genetic features of South Korean FCV isolates. Crandell-Rees feline kidney (CRFK) cells were used to isolate FCV from 58 organ homogenate samples. The FCV isolates were confirmed by cytopathic effects, immunofluorescence, electron microscopy, and reverse transcription polymerase chain reaction assays. Viral genetic analysis was carried out with VP2 gene and complete genomes of FCVs. Five viruses propagated in CRFK cells were confirmed to be FCVs. The FCV17D283 isolate showed the highest viral titer of 107.2 TCID50/mL at 36 h post-inoculation. Korean FCV isolates did not grow well in Vero, BHK-21, A72, or Madin-Darby canine kidney cells. The FCV17D03 and FCV17D283 isolates had the highest genetic similarity (80.1% and 86.9%) with the UTCVM-H1 and 14Q315 strains, which were isolated in the United States and South Korea in 1995 and 2014, respectively. We isolated five FCVs from cats and detected important genetic differences among them. FCV isolates did not show any virulent effects in mice.

Expression of the VP2 protein of feline panleukopenia virus in insect cells and use thereof in a hemagglutination inhibition assay

Yang, Dong-Kun,Park, Yeseul,Park, Yu-Ri,Yoo, Jae Young,An, Sungjun,Park, Jungwon,Hyun, Bang-Hun The Korean Society of Veterinary Science 2021 大韓獸醫學會誌 Vol.61 No.2

Feline panleukopenia virus (FPV) causes leukopenia and severe hemorrhagic diarrhea, killing 50% of naturally infected cats. Although intact FPV can serve as an antigen in the hemagglutination inhibition (HI) test, an accidental laboratory-mediated infection is concern. A non-infectious diagnostic reagent is required for the HI test. Here, we expressed the viral protein 2 (VP2) gene of the FPV strain currently prevalent in South Korea in a baculovirus expression system; VP2 protein was identified by an indirect immunofluorescence assay, electron microscopy (EM), Western blotting (WB), and a hemagglutination assay (HA). EM showed that the recombinant VP2 protein self-assembled to form virus-like particles. WB revealed that the recombinant VP2 was 65 kDa in size. The HA activity of the recombinant VP2 protein was very high at 1:2¹⁵. A total of 143 cat serum samples were tested using FPV (HI-FPV test) and the recombinant VP2 protein (HI-VP2 test) as HI antigens. The sensitivity, specificity, and accuracy of the HI-VP2 test were 99.3%, 88.9%, and 99.3%, respectively, compared to the HI-FPV test. The HI-VP2 and HI-FPV results correlated significantly (r = 0.978). Thus, recombinant VP2 can substitute for intact FPV as the serological diagnostic reagent of the HI test for FPV.

Expression of TRP calcium channels in human periodontal ligament cells and periodontitis mice

Ae Ri Kim,Aeryun Kim,Yu-Mi Yang,Yun-Jung Yoo,Eun-Jung Bak 한국실험동물학회 2019 한국실험동물학회 학술발표대회 논문집 Vol.2019 No.1

Immunohistochemical localization of glucose transporter 1 and 3 in the scrotal and abdominal testes of a dog

Kyu Ri Hahn,Hyo Young Jung,Dae Young Yoo,Jong Whi Kim,Yang Hee Kim,Young Kwang Jo,Geon A Kim,Jin Young Chung,Jung Hoon Choi,In Koo Hwang,Goo Jang,Yeo Sung Yoon 한국실험동물학회 2017 Laboratory Animal Research Vol.33 No.2

Glucose is essential for testicular function; the uptake of carbohydrate-derived glucose by cells is mediated by glucose transporters (GLUTs). In the present study, we investigated the activity of GLUT1 and GLUT3, the two main isoforms of GLUTs found in testes, in the left scrotal and right abdominal testes of a German Shepherd dog. Immunohistochemical analysis showed that GLUT1 immunoreactivity was absent in the scrotal and abdominal testes. In contrast, weak to moderate GLUT3 immunoreactivity was observed in mature spermatocytes as well as spermatids in the scrotal testis. In the abdominal testis, relatively strong GLUT3 immunoreactivity was detected in Leydig cells only and was absent in mature spermatocytes and spermatids. GLUT3 immunoreactivity was significantly decreased in the tubular region of abdominal testis and significantly increased in the extra-tubular (interstitial) region of abdominal testis compared to observations in the each region of scrotal testis, respectively. These results suggest that GLUT3 is the major glucose transporter in the testes and that abdominal testes may increase the uptake of glucose into interstitial areas, leading to an increased risk of developing cancer.

염기성 촉매를 이용한 적색 산화구리 안료의 형상 제어 및 열적 특성 평가

유리 ( Ri Yu ),윤지연 ( Ji Yeon Yun ),양희승 ( Hee Seung Yang ),피재환 ( Jae Hwan Pee ),김유진 ( Yoo Jin Kim ) 대한금속재료학회(구 대한금속학회) 2015 대한금속·재료학회지 Vol.53 No.2

This paper reports the tunable structural design and thermal characteristics of a SiO2 layer on Cu2O pigment via a dual-effect of NH4OH catalyst. We prepared octahedron Cu2O 200~300 nm in size and successfully synthesized a core-shell Cu2O@SiO2 structure using a modified Stober method in which the NH4OH concentration was adjusted. The NH4OH solution dipping time was controlled such that the Cu2O core was generated with a yolk-shell and a hollow-shell. The silica-coated Cu2O raw materials were calcined at various temperatures (150~500 ℃). The thermal and color effects of the silica on the Cu2O were characterized by scanning electron microscopy, CIE L*a*b* color parameter measurements, transmission electron microscopy, and X-ray diffraction.

Different roles of surveillance positron emission tomography according to the histologic subtype of non-Hodgkin’s lymphoma

( Yu Ri Kim ),( Soo-jeong Kim ),( June-won Cheong ),( Yundeok Kim ),( Ji Eun Jang ),( Hyunsoo Cho ),( Haerim Chung ),( Yoo Hong Min ),( Woo Ick Yang ),( Arthur Cho ),( Jin Seok Kim ) 대한내과학회 2021 The Korean Journal of Internal Medicine Vol.36 No.0

Background/Aims: Although the use of surveillance 18F-fluorodeoxyglucose (FDG) positron emission tomography/computed tomography (PET/CT) is discouraged in patients with diffuse large B-cell lymphoma, its usefulness in different subtypes has not been thoroughly investigated. Methods: We retrospectively evaluated 157 patients who showed positive results on surveillance FDG-PET/CT every 6 months following complete response for up to 5 years. All of the patients also underwent biopsies. Results: Seventy-eight (49.6%) of 157 patients had true positive results; the remaining 79 (50.3%), including eight (5.1%) with secondary malignancies, were confirmed to yield false positive results. Among the 78 patients with true positive results, the disease in seven (8.9%) had transformed to a different subtype. The positive predictive value (PPV) of FDG-PET/CT for aggressive B-cell non-Hodgkin’s lymphoma (NHL) was lower than that for indolent B-cell or aggressive T-cell NHL (p = 0.003 and p = 0.018, respectively), especially in patients with a low/low-intermediate international prognostic index (IPI) upon a positive PET/CT finding. On the other hand, indolent B-cell and aggressive T-cell NHL patients showed PPVs of > 60%, including those with low/low-intermediate secondary IPIs. Conclusions: The role of FDG-PET/CT surveillance is limited, and differs according to the lymphoma subtype. FDG-PET/CT may be useful in detecting early relapse in patients with aggressive T-cell NHL, including those with low/low-intermediate risk secondary IPI; as already known, FDG-PET/CT has no role in aggressive B-cell NHL. Repeat biopsy should be performed to discriminate relapse or transformation from false positive findings in patients with positive surveillance FDG-PET/CT results.

Immunogenicity of an inactivated rabies vaccine for animals derived from the recombinant ERAGS strain

Dong-Kun Yang,Ha-Hyun Kim,Yu-Ri Park,Jae Young Yoo,Yeseul Park,Sungjun An,Bang-Hun Hyun 대한백신학회 2021 Clinical and Experimental Vaccine Research Vol.10 No.2

Purpose: The aims of the present study were to evaluate the immunogenicity of an inactivated rabies vaccine based on the ERAGS strain. Materials and Methods: The ERAGS virus propagated in Vero cells was inactivated with 3 mM binary ethylenimine for 8 hours. Three types of inactivated rabies vaccines were prepared to determine the minimum vaccine virus titers. Four further types of inactivated rabies vaccines were prepared by blending inactivated ERAGS with four different adjuvants; each vaccine was injected into mice, guinea pigs, and dogs to identify the optimal adjuvant. The immunogenicity of a Montanide (IMS) gel-adjuvanted vaccine was evaluated in cats, dogs, and cattle. Humoral immune responses were measured via a fluorescent antibody virus neutralization method and a blocking enzyme-linked immunosorbent assay. Results: The minimum virus titer of the inactivated rabies vaccine was over 107.0 50% tissue culture infectious doses (TCID50 values)/mL. Of the four kinds of adjuvants, the IMS gel-adjuvanted vaccine induced the highest mean viral neutralizing antibody (VNA) titers of 6.24 and 2.36 IU/mL in guinea pigs and dogs, respectively, and was thus selected as the vaccine for the target animals. Cats, dogs, and cattle inoculated with the IMS gel-adjuvanted vaccine developed protective VNA titers ranging from 3.5 to 1.2 IU/mL at 4 weeks post-inoculation (WPI). Conclusion: Our data indicate that cats, dogs, and cattle inoculated with an inactivated rabies vaccine derived from the ERAGS strain developed protective immune responses that were maintained to 12 WPI.

Generation of a recombinant rabies virus expressing green fluorescent protein for a virus neutralization antibody assay

Dong-Kun Yang,Ha-Hyun Kim,Yu-Ri Park,Jae-Young Yoo,Yeseul Park,Jung-Won Park,Bang-Hun Hyun 대한수의학회 2021 Journal of Veterinary Science Vol.22 No.4

Background: Fluorescent antibody virus neutralization (FAVN) test is a standard assay for quantifying rabies virus-neutralizing antibody (VNA) in serum. However, a safer rabies virus (RABV) should be used in the FAVN assay. There is a need for a new method that is economical and time-saving by eliminating the immunostaining step. Objectives: We aimed to improve the traditional FAVN method by rescuing and characterizing a new recombinant RABV expressing green fluorescent protein (GFP). Methods: A new recombinant RABV expressing GFP designated as ERAGS-GFP was rescued using a reverse genetic system. Immuno-fluorescence assay, peroxidase-linked assay, electron microscopy and reverse transcription polymerase chain reaction were performed to confirm the recombinant ERAGS-GFP virus as a RABV expressing the GFP gene. The safety of ERAGS-GFP was evaluated in 4-week-old mice. The rabies VNA titers were measured and compared with conventional FAVN and FAVN-GFP tests using VERO cells. Results: The virus propagated in VERO cells was confirmed as RABV expressing GFP. The ERAGS-GFP showed the highest titer (108.0 TCID50/mL) in VERO cells at 5 days post-inoculation, and GFP expression persisted until passage 30. The body weight of 4-week-old mice inoculated intracranially with ERAGS-GFP continued to increase and the survival rate was 100%. In 62 dog sera, the FAVN-GFP result was significantly correlated with that of conventional FAVN (r = 0.95). Conclusions: We constructed ERAGS-GFP, which could replace the challenge virus standard-11 strain used in FAVN test.