Effects of Ozone on the Cytomembrane and Ultrastructure of Pseudomonas aeruginosa

Yong-qing Zhang,Qing-ping Wu,Ju-mei Zhang,Xiu-hua Yang 한국식품과학회 2015 Food Science and Biotechnology Vol.24 No.3

The ozone impact on Pseudomonas aeruginosa ATCC27853 cells was studied. Ozone-induced loss of K+, Mg2+, and ATP was measured using inductively coupled plasma/mass spectrometry and a bioluminescence assay. Maximum releases of K+ and Mg2+ were achieved at 0.37 mg/L of ozone after 2 min with a killing rate of culturable bacteria greater than 93%. Maximum release of ATP was attained at 0.42 mg/L of ozone after 3 min. Transmission electron microscopy showed that shapes of treated cells were integrated, but cytoplasmic agglutinations and vacuoles appeared. Ozone treatment caused lysis of P. aeruginosa cells to be sensitive to sodium dodecyl sulfate, insensitive to NaOH, and subject to inactivation by proteinase K. A combined action of cytoplasm agglutination, protein denaturation, and membrane permeabilization, rather than cell lysis, leads to non-culturability of P. aeruginosa cells.

Molecular Analysis of the Copper-Responsive CopRSCD of a Pathogenic Pseudomonas fluorescens Strain

Yong-hua Hu,Hua-lei Wang,Min Zhang,Li Sun 한국미생물학회 2009 The journal of microbiology Vol.47 No.3

CopRS/CopABCD is one of the known systems that control copper homeostasis in bacteria. Although CopRS/CopABCD homologues are found to exist in Pseudomonas fluorescens, the potential role of this system in P. fluorescens has not been investigated. In this study a genetic cluster, consisting of copR, S, C, and D but lacking copAB, was identified in a pathogenic P. fluorescens strain (TSS) isolated from diseased fish. The copRSCD cluster was demonstrated to be required for full copper resistance and regulated at the transcription level by Cu. Expression of copCD is regulated directly by the two-component response regulator CopR, which also regulates its own expression. Interruption of the regulated expression of copR affected bacterial growth, biofilm formation, and tissue dissemination and survival. A mutant CopR, which lacks the N-terminal signal receiver domain and is constitutively active, was found to have an attenuating effect on bacterial virulence when expressed in TSS. To our knowledge, this is the first report that suggests a link between CopR and bacterial pathogenicity in P. fluorescens.

Molecular characterization and expression analysis of putative odorant carrier proteins in Adelphocoris lineolatus

Yong Xiao,Liang Sun,Qi Wang,Qiang Zhang,Shao-Hua Gu,Adel Khashaveh,Ze-Wen Liu,Yong-Jun Zhang 한국응용곤충학회 2018 Journal of Asia-Pacific Entomology Vol.21 No.3

The alfalfa plant bug Adelphocoris lineolatus (Goeze) (Hemiptera: Miridae), a serious pest of several agricultural crops, is extremely attracted by flowering plants to perform season host switching. Generally, chemoreception of A. lineolatus plays a crucial role in detecting food sources, locating mates and oviposition sites. In addition, odorant carrier proteins are believed to participate in the initial chemical communication and perception in insects. In this work, we identified 31 putative odorant carrier protein genes from the antennal transcriptomes of A. lineolatus, including 17 new odorant binding proteins (OBPs), 10 new chemosensory proteins (CSPs) and four Niemann-Pick type C2 proteins (NPC2s). Phylogenetic analysis demonstrated that the OBP and CSP genes from four mirid bug species (A. lineolatus, Adelphocoris suturalis, Apolygus lucorum and Lygus lineolaris) had a relatively close evolutionary relationship. Regular patterns and key conserved motifs of OBPs and CSPs in Hemiptera insects are also identified by using Multiple EM for Motif Elicitation (MEME) tool. Tissue expression profiles analysis showed that nine of the 17 OBPs, 10 CSPs and two of the four NPC2s were primarily or uniquely expressed in the adult antennae suggesting their putative roles in chemoreception. Our study provides the first insight into the roles of odorant carrier proteins in chemoreception of A. lineolatus, which will help to facilitate biological functions of odorant carrier proteins and develop novel insect behavioral regulation strategy

PREPARATION OF CINOBUFAGIN-LOADED BOVINE SERUM ALBUMIN NANOPARTICLES FOR HEPATOCARCINOMA THERAPY

YONG-HUA SU,JIAN-GUO ZHANG,JIE SHEN,FENG-QIAN LI,HUA SU,CHANG-QUAN LING 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2009 NANO Vol.4 No.1

Cinobufagin-loaded bovine serum albumin nanoparticles were prepared for treating hepatocellular carcinoma. In this report, cinobufagin-bovine serum albumin-nanoparticles (Cino-BSA-NP) were prepared by an aqueous desolvation process. The physicochemical properties, toxicity, and cancer-related applications of Cino-BSA-NP were investigated. Cino-BSA-NP had a uniform spherical morphology with a particle size in the range of 50–240 nm and an average size of 86.3 nm. The zeta potential of the nanoparticles was -49 mV. The overall embedding ratio was 79.5% and the drug loading was 24.1%. Cino-BSA-NP gave cinobufagin release of up to 53.5% within 3 h, followed by slower controlled release. Cino-BSA-NP inhibited growth of hepatocarcinoma cells in vitro to a similar extent as free cinobufagin, but with a much higher median lethal dose (LD50). Hepatic histomorphological changes indicated that hepatic damage was much less severe with Cino-BSA-NP than with free cinobufagin (2.19 mg/kg). The survival time of nude mice with orthotopic transplantation tumors treated with Cino-BSA-NP was prolonged significantly. The results confirm that Cino-BSA-NP renders cinobufagin completely dispersible in aqueous media, meeting the key requirements for intravenous injection, and show controlled release, thus significantly improving cinobufagin's antitumor activity while reducing its side effects.

Reduction of Brain Infarct Size and Extracellular Glutamate Accumulation by Dihydrokinate in Spontaneously Hypertensive Rat after Transient Forebrain Ischemia

Yong Hua Zhang,Yong Cai Li,Zheng Hua Cai,Yang Chen 대한신경외과학회 2004 Journal of Korean neurosurgical society Vol.36 No.4

Objective : Using bilateral carotid artery occlusion, controlled hypotension and in vivo microdialysis, we evaluate the changes in extracellular concentrations of the excitatory amino acids(EAA) glutamate and aspartate in the striatum of ischemic Wistar-Kyoto rats(WKY) and spontaneously hypertensive rats (SHR), as well as the resulting changes of infarct volume and neurological deficit scores. Methods : During 30 minutes of ischemia, microdialysate concentrations of glutamate and aspartate were measured in the presence of infusion of artificial cerebrospinal fluid(aCSF) or the dihydrokinate (DHK, the GLT-1 selective nontransportable inhibitor)-containing aCSF respectively. And infarct volume of neurological deficit were measured for each groups using triphenyltetrazolium chloride(TTC) staining and neurological deficit function tests. Results : In control aCSF infusing WKY group, glutamate and aspartate concentrations increased 36-fold and 14- fold increase to baseline, respectively, and returned to baseline values on reperfusion; while in control aCSF infusing SHR group, glutamate and aspartate increased 51-fold and 25-fold increase respectively. DHK (10nM) significantly attenuated EAA increases in both experimental WKY group and SHR group compared with control (glutamate peak for WKY, 7.09±1.04 versus control, 18.48±1.87 pmol/ul; for SHR, 18.90±1.54 versus control, 40.64±2.38 pmol/ul). There is a significant improving with neurological deficit score in DHK application groups both in WKY and SHR, which was also convinced by comparison of infarct volume between experimental DHK infusing group and control. Conclusion : Our results suggest that the astrocytic glutamate transporter contributes significantly to EAA release in the early phase of ischemia and inhibition of excessive glutamate release by DHK may contribute to effective treatment in ischemia-induced brain damage.

Tracking the movement trajectory of newly emerged adult Chinese citrus flies with insect harmonic radar

Zhang-Zhang He,Jie Luo,Lian-You Gui,Deng-Ke Hua,Tian-Hua Du,Fu-Lian Wang,Peng Liang,Yong-Fang Shi,Xuan Yang 한국응용곤충학회 2019 Journal of Asia-Pacific Entomology Vol.22 No.3

Previous work reveals that the adult Chinese citrus fly, Bactrocera minax (Enderlein), emerges from the ground in citrus orchards and presumably disperses to alternate food sources in neighboring shrubs and trees, where it stays and becomes reproductively mature, and then later returns to the orchard to lay eggs. We investigated the trajectories of early emerged adult Chinese citrus flies, which were tracked with portable harmonic radar in 2013, 2014, 2015, and 2016 in four different habitats in Hubei province of China that presumably provided food sources for adults. This study we investigated whether early emerged adult Chinese citrus flies migrate into forests. A total of 47 adults were tracked following release in a citrus orchard. Our results showed that a total of 33 adults released in a citrus orchard flew into the forest, and 14 adults that disappeared during the tracking process in the citrus orchard. A total of 10 adults (in 2014) that were tracked and released in the forest flew to the forest. Of the adult flies investigated, 54.5% flew directly into the forest, while the remaining 45.5% finally arrived in the forest after repeatedly adjusting their trajectories. When there were Castanea mollissima Blume (Fagaceae) in the surrounding forest, the proportion of frequency of adult landing on the plant represented 58.3% and 88.5% of landing on all species in 2014 and in 2015, respectively. We conclude that early emerged adult Chinese citrus flies in the mosaic-type citrus orchard of the hilly terrain landform dispersed into adjacent forest.

Fate and Bioaccumulation of Polynuclear Aromatic Hydrocarbons in Coastal Sediment

Yong Zhang,Ya-Xian Zhu,Jie-Zhang,Feng Ren,Feng-Lan Zheng,Sang-Jin Kim(김상진),H. W. Lam,Hong Hua-sheng,S. S. Wu 한국해양환경·에너지학회 2001 한국해양환경·에너지학회 학술대회논문집 Vol.2001 No.-

Identification of Polymorphisms in Thymic Stromal Lymphopoietin (TSLP) Gene and Their Association with Allergic Rhinitis

Mei Hua Zhang,Yong Shin Kim,Eun Heui Jin,Ki Mo Kim,Jae Hoon Lee,Chun Shi Li,Qing Gao Zhang,Ki Jung Yun,Soo Cheon Chae,Hun Taeg Chung 한국유전학회 2008 Genes & Genomics Vol.30 No.5

Dendritic cell (DC) activator, thymic stromal lymphopoietin (TSLP) induces DCs to produce Th2-attracting chemokines, causing differentiation of CD4+ and CD8+ T cells into effector cells. The differentiated cells are characterized by a typical pro-allergic phenotype. In this study, we identified four single nucleotide polymorphisms (SNPs) and one variation site (g.-305delT) from the results of scanned human TSLP gene. The SNPs are as following: g.-1914A>G and g.-847C>T in promoter, g.-82C>T in 5` UTR and g.1117C>T in intron 2. We also evaluated the association of genotype and allele frequencies of these SNPs between non-allergic rhinitis controls and allergic rhinitis patients. We further investigated possible correlations between each genotype with serum total IgE levels and peripheral blood eosinophil counts in allergic rhinitis patients. The frequencies of haplotype constructed by these SNPs between these two groups were also compared. Our results demonstrate the two SNPs (g.-1914A>G and g.-847C>T) were associated with susceptibility of allergic rhinitis (P=0.006 and P=0.015 respectively), however, serum total IgE levels were not significantly related with the SNPs.

Identification of Specific Gene Modules in Mouse Lung Tissue Exposed to Cigarette Smoke

Xing, Yong-Hua,Zhang, Jun-Ling,Lu, Lu,Li, De-Guan,Wang, Yue-Ying,Huang, Song,Li, Cheng-Cheng,Zhang, Zhu-Bo,Li, Jian-Guo,Xu, Guo-Shun,Meng, Ai-Min Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.10

Background: Exposure to cigarette may affect human health and increase risk of a wide range of diseases including pulmonary diseases, such as chronic obstructive pulmonary disease (COPD), asthma, lung fibrosis and lung cancer. However, the molecular mechanisms of pathogenesis induced by cigarettes still remain obscure even with extensive studies. With systemic view, we attempted to identify the specific gene modules that might relate to injury caused by cigarette smoke and identify hub genes for potential therapeutic targets or biomarkers from specific gene modules. Materials and Methods: The dataset GSE18344 was downloaded from the Gene Expression Omnibus (GEO) and divided into mouse cigarette smoke exposure and control groups. Subsequently, weighted gene co-expression network analysis (WGCNA) was used to construct a gene co-expression network for each group and detected specific gene modules of cigarette smoke exposure by comparison. Results: A total of ten specific gene modules were identified only in the cigarette smoke exposure group but not in the control group. Seven hub genes were identified as well, including Fip1l1, Anp32a, Acsl4, Evl, Sdc1, Arap3 and Cd52. Conclusions: Specific gene modules may provide better understanding of molecular mechanisms, and hub genes are potential candidates of therapeutic targets that may possible improve development of novel treatment approaches.

ACCURACY OF LAMOST DR1 STELLAR PARAMETERS

GAO, HUA,ZHANG, HUA-WEI,XIANG, MAO-SHENG,HUANG, YANG,LIU, XIAO-WEI,LUO, A-LI,ZHANG, HAO-TONG,WU, YUE,ZHANG, YONG,LI, GUANG-WEI,DU, BING The Korean Astronomical Society 2015 天文學論叢 Vol.30 No.2

We adopt the PASTEL catalog combined with SIMBAD radial velocities as a testing standard to validate the stellar parameters (effective temperature $T_{eff}$, surface gravity log g, metallicity [Fe/H] and radial velocity $V_r$) from the first data release (DR1) of The Large Sky Area Multi-Object Fiber Spectroscopic Telescope (LAMOST) survey. After applying data reduction and temperature constraints to the sample obtained by cross-identification, we compare the stellar parameters from DR1 and PASTEL. The results show that the DR1 results are reliable under certain conditions. We derive a dispersion of 110 K, 0.19 dex, 0.11 dex and $4.91kms^{-1}$ in specified effective temperature ranges, for $T_{eff}$, log g, [Fe/H] and $V_r$ respectively. Systematic errors are negligible except for those of $V_r$. In addition, for stars with PASTEL [Fe/H] < -1:5, the metallicities in DR1 are systematically higher than those in PASTEL.