A simple unstructured model-based control for efficient expression of recombinant porcine insulin precursor by Pichia pastoris

Siliang Zhang,Hai-feng Hang,Wen Chen,Mei-jin Guo,Ju Chu,Ying-ping Zhuang 한국화학공학회 2008 Korean Journal of Chemical Engineering Vol.25 No.5

Based on the fact that Pichia cell growth follows a Monod equation under the condition of methanol concentration limitation, a kinetics model of recombinant methylotrophic yeast Pichia pastoris expressing porcine insulin precursor (PIP) was developed in the quasi-steady state in the induction phase. The model revealed that the relationship between specific growth rate (μ) and substrate methanol concentration was in accord with the Monod equation. The fermentation kinetic parameters maximum specific growth rate (μmax), saturation constant (Ks) and maintenance coefficient (M) were estimated to be 0.101 h−1, 0.252 g l−1, and 0.011 g MeOH g−1 DCW h−1, respectively. The unstructured model was validated in methanol induction phase with different initial cell densities. Results showed that the maximum specific protein production rate (qp.max) of 0.098 mg g−1 DCW h−1 was achieved when μ was kept at 0.016 h−1, and the maximum yield of PIP reached 0.97 g l−1, which was 1.5-fold as that of the control. Therefore, the simple Monod model proposed has proven to be a robust control system for recombinant porcine insulin precursor production by P. pastoris on pilot scale, which would be further applied on production scale.

Fatty Acid Binding Protein 5 (FABP5) Promotes Aggressiveness of Gastric Cancer Through Modulation of Tumor Immunity

Shu Zhang,Mei-qing Qiu,Hui-jun Wang,Ya-fei Ju,Zhen Liu,Tao Wang,Shi-feng Kan,Zhen Yang,Ya-yun Cui,You-qiang Ke,Hong-min He,Li Sun 대한위암학회 2023 Journal of gastric cancer Vol.23 No.2

Purpose: Gastric cancer (GC) is the second most lethal cancer globally and is associated with poor prognosis. Fatty acid-binding proteins (FABPs) can regulate biological properties of carcinoma cells. FABP5 is overexpressed in many types of cancers; however, the role and mechanisms of action of FABP5 in GC remain unclear. In this study, we aimed to evaluate the clinical and biological functions of FABP5 in GC. Materials and Methods: We assessed FABP5 expression using immunohistochemical analysis in 79 patients with GC and evaluated its biological functions following in vitro and in vivo ectopic expression. FABP5 targets relevant to GC progression were determined using RNA sequencing (RNA-seq). Results: Elevated FABP5 expression was closely associated with poor outcomes, and ectopic expression of FABP5 promoted proliferation, invasion, migration, and carcinogenicity of GC cells, thus suggesting its potential tumor-promoting role in GC. Additionally, RNA-seq analysis indicated that FABP5 activates immune-related pathways, including cytokine-cytokine receptor interaction pathways, interleukin-17 signaling, and tumor necrosis factor signaling, suggesting an important rationale for the possible development of therapies that combine FABP5-targeted drugs with immunotherapeutics. Conclusions: These findings highlight the biological mechanisms and clinical implications of FABP5 in GC and suggest its potential as an adverse prognostic factor and/or therapeutic target.

Altered Distribution and Expression Pattern of E-cadherin in Hepatocellular Carcinomas: Correlations with Prognosis and Clinical Features

Jiang, Xue-Mei,Zhang, Ju-Bo,Xiong, Ju,Huang, Xiao-Xi,Ren, Zheng-Gang Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.12

Objective: E-cadherin has been identified as a tumor suppressor in many types of carcinoma. However, some studies recently suggested that the role and expression of E-cadherin might be more complex and diverse. In the present study, we evaluated the prognostic value of E-cadherin expression with reference to levels in membranes and cytoplasm, and the membrane/cytoplasm ratio, in hepatocellular carcinomas (HCCs) after curative hepatectomy. Methods: The expression of E-cadherin was assessed by immunohistochemistry in HCC tissue microarrays from 125 patients, and its prognostic values and other clinicopathlogical data were retrospectively analyzed. Patients were followed for a median period of 43.7 months (range 1 to 126 months). Results: Univariate analysis demonstrated that a high membrane/cytoplasm (M/C) ratio of E-cadherin expression was associated with poor overall survival (OS) (P =0.001) and shorter time to recurrence (TTR) (P=0.038), as well as tumor size, intrahepatic metastasis, and TNM stage. In contrast, neither membrane nor cytoplasmic expression of E-cadherin was related with OS and TTR. Furthermore, multivariate analysis confirmed the M/C ratio to be an independent predictor of OS (P=0.031). ${\chi}^2$ tests additionally showed that the M/C ratio of E-cadherin expression was related with early stage recurrence (P=0.012), rather than later stage recurrence. Conclusion: The M/C ratio of E-cadherin expression is a strong predictor of postoperative survival and is associated with early stage recurrence in patients with HCC.

Effects of Ozone on the Cytomembrane and Ultrastructure of Pseudomonas aeruginosa

Yong-qing Zhang,Qing-ping Wu,Ju-mei Zhang,Xiu-hua Yang 한국식품과학회 2015 Food Science and Biotechnology Vol.24 No.3

The ozone impact on Pseudomonas aeruginosa ATCC27853 cells was studied. Ozone-induced loss of K+, Mg2+, and ATP was measured using inductively coupled plasma/mass spectrometry and a bioluminescence assay. Maximum releases of K+ and Mg2+ were achieved at 0.37 mg/L of ozone after 2 min with a killing rate of culturable bacteria greater than 93%. Maximum release of ATP was attained at 0.42 mg/L of ozone after 3 min. Transmission electron microscopy showed that shapes of treated cells were integrated, but cytoplasmic agglutinations and vacuoles appeared. Ozone treatment caused lysis of P. aeruginosa cells to be sensitive to sodium dodecyl sulfate, insensitive to NaOH, and subject to inactivation by proteinase K. A combined action of cytoplasm agglutination, protein denaturation, and membrane permeabilization, rather than cell lysis, leads to non-culturability of P. aeruginosa cells.

On-line Specific Growth Rate Control for Improving Reduced Glutathione Production in Saccharomyces cerevisiae

Zhi-Qiang Xiong,Mei-jin Guo,Ju Chu,Ying-ping Zhuang,Siliang Zhang 한국생물공학회 2015 Biotechnology and Bioprocess Engineering Vol.20 No.5

Reduced glutathione (GSH), the abundant bioactive tripeptide in most living cells, is widely used in pharmaceutical, food, and cosmetic industries. Specific growth rate (μ) is a key physiological parameter for GSH high-cell-density cultivation using microbial cell factories. Here, based on a biomass probe, an on-line μ feedback control was developed to regulate glucose feeding rate during the fed-batch phase for overproducing GSH in Saccharomyces cerevisiae. Compared with real-time μ controlled at 0.15/h, μ controlled at 0.2/h achieved yeast dry weight (120 g/L), GSH concentration (1.5 g/L), and intracellular GSH content (1.25%), which improved by 9, 150, and 129.1%, respectively. To our knowledge, this is the first report about on-line μ feedback control for GSH production. On-line μ control led to 59.38 mg/L/h of GSH productivity and 3.52 mg/g of GSH yield on glucose, which improved by 107.6 and 7.2%, respectively, in comparison with those of traditional ethanol feedback control (maintaining ethanol concentration at 1%). Taken together, the on-line μ feedback control is a promising method as an efficient alternative to conventional feed control techniques presently practiced in the GSH industry, and has the potential for the production of other valuable chemicals.

Anticancer Effects of 23-Dihydroganoderic Acid N

Kang Kyoung-Ah,Zhang Rui,Piao Mei Jing,Kim Ju-Sun,Kang Sam-Sik,Hyun Jin-Won Korean Environmental Mutagen Society 2006 한국환경성돌연변이·발암원학회지 Vol.26 No.4

23-Dihydroganoderic acid N, a triterpenoid compound, was investigated whether it may show cytotoxic activity against U937, HeLa, NCI-H460 and MCF-7 cancer cells by MTT test. As a result, 23-dihydroganoderic acid N show sensitive to MCF-7 cells among NCI-H460, HeLa, U937, and MCF-7 cells.

Dynamic Change and Stability of the Pigments in Prunus × Cistena 'Pissardii'

Xiang Shen,Fu mei Lv,Yan li. Hu,Qing ju Wang,Xiao yan Zhang 한국원예학회 2006 한국원예학회 기타간행물 Vol.- No.-

Classification and fatty acid composition analysis of Cronobacter spp. isolated from powdered infant formula in China

Xiaojuan Yang,Qing-Ping Wu,Ju-Mei Zhang,Weipeng Guo,Shuping Mo,Sheng-Rong Liu 한국식품과학회 2016 Food Science and Biotechnology Vol.25 No.4

This study aimed to classify a collection of Enterobacter sakazakii (E. sakazakii) strainspreviously identified from powdered infant formula (PIF) to species level by recN gene sequencing andbiochemical testing to determine the distribution of Cronobacter species in China and investigate thestrain diversity by cellular fatty acid (CFA) analysis. Of 24 E. sakazakii isolates, 23 were identified as C. sakazakii and one was C. malonaticus. The 23 C. sakazakii isolates showed the same CFA profiles. TheC. malonaticus isolate was discriminated from the C. sakazakii isolates by the significant difference inthe amounts of C12:0, C14:0, and C17:0 cyclo acids. These results showed that C. sakazakii and C. malonaticuswere the common Cronobacter species distributed in PIF in China and that the isolates of the twospecies exhibited different CFA profiles. These findings are of value for epidemiological investigationsand provide an alternative method for confirming various Cronobacter spp.

Antioxidant and Cytotoxicity Effects of Luteolin

Park Jin-Ny,Kang Kyoung-Ah,Zhang Rui,Piao Mei Jing,Park So-Yoon,Kim Ju-Sun,Kang Sam-Sik,Hyun Jin-Won Korean Society of ToxicologyKorea Environmental Mu 2006 Toxicological Research Vol.22 No.4

Flavonoids are polyphenolic compounds that are ubiquitous in plants. They have been shown to possess a variety of biological activities, such as antioxidant and anticancer. Reactive oxygen species (ROS) lead to damages of cellular molecules and it is the one of various factors to induce cancer. The one of flavonoids, Luteolin, was found to scavenge 1,1-diphenyl-2-piculhydrazyl (DPPH) radical and intracellular reactive oxygen species (ROS). Moreover luteolin showed protection on hamster lung fibroblast cells (V79-4 cell) damage induced by $H_2O_2$. And then it was investigated whether it may show cytotoxicity effect against various cancer cells by MTT, Luteolin at $10{\mu}g/ml$ showed the cell viability of 63.2%, 34.7%, 18.4% and 71.4% against NCl-H460, HeLa, U937 and MCF-7, respectively. As a result, luteolin shows more sensitive to U937 cells among the tested cancer cell lines. In summary, luteolin has antioxidant and cytotoxicity effect or various cancer cell lines.

Antioxidant and Cytotoxicity Effects of Luteolin

Jinny Park,Kyoung Ah Kang,Rui Zhang,Mei Jing Piao,Soyoon Park,Ju Sun Kim,Sam Sik Kang,Jin Won Hyun 한국독성학회 2006 Toxicological Research Vol.22 No.4

Flavonoids are polyphenolic compounds that are ubiquitous in plants. They have been shown to possess a variety of biological activities, such as antioxidant and anticancer. Reactive oxygen species (ROS) lead to damages of cellular molecules and it is the one of various factors to induce cancer. The one of flavonoids, Luteolin, was found to scavenge 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical and intracellular reactive oxygen species (ROS). Moreover luteolin showed protection on hamster lung fibroblast cells (V79-4 cell) damage induced by H₂O₂. And then it was investigated whether it may show cytotoxicity effect against various cancer cells by MTT. Luteolin at 10 ㎍/㎖ showed the cell viability of 63.2%, 34.7%, 18.4% and 71.4% against NCI-H460, HeLa, U937 and MCF-7, respectively. As a result, luteolin shows more sensitive to U937 cells among the tested cancer cell lines. In summary, luteolin has antioxidant and cytotoxicity effect on various cancer cell lines.