Applications of Bacterial Cellulose-Based Composite Materials in Hard Tissue Regenerative Medicine

Liu Yingyu,Liu Haiyan,Guo Susu,Qi Jin,Zhang Ran,Liu Xiaoming,Sun Lingxiang,Zong Mingrui,Cheng Huaiyi,Wu Xiuping,Shanxi Medical University School and Hospital of Stomatology 한국조직공학과 재생의학회 2023 조직공학과 재생의학 Vol.20 No.7

BACKGROUND: Cartilage, bone, and teeth, as the three primary hard tissues in the human body, have a significant application value in maintaining physical and mental health. Since the development of bacterial cellulose-based composite materials with excellent biomechanical strength and good biocompatibility, bacterial cellulose-based composites have been widely studied in hard tissue regenerative medicine. This paper provides an overview of the advantages of bacterial cellulose-based for hard tissue regeneration and reviews the recent progress in the preparation and research of bacterial cellulose-based composites in maxillofacial cartilage, dentistry, and bone. METHOD: A systematic review was performed by searching the PubMed and Web of Science databases using selected keywords and Medical Subject Headings search terms. RESULTS: Ideal hard tissue regenerative medicine materials should be biocompatible, biodegradable, non-toxic, easy to use, and not burdensome to the human body; In addition, they should have good plasticity and processability and can be prepared into materials of different shapes; In addition, it should have good biological activity, promoting cell proliferation and regeneration. Bacterial cellulose materials have corresponding advantages and disadvantages due to their inherent properties. However, after being combined with other materials (natural/ synthetic materials) to form composite materials, they basically meet the requirements of hard tissue regenerative medicine materials. We believe that it is worth being widely promoted in clinical applications in the future. CONCLUSION: Bacterial cellulose-based composites hold great promise for clinical applications in hard tissue engineering. However, there are still several challenges that need to be addressed. Further research is needed to incorporate multiple disciplines and advance biological tissue engineering techniques. By enhancing the adhesion of materials to osteoblasts, providing cell stress stimulation through materials, and introducing controlled release systems into matrix materials, the practical application of bacterial cellulose-based composites in clinical settings will become more feasible in the near future.

Complete genome and phylogenetic analysis of bovine papillomavirus type 15 in Southern Xinjiang dairy cow

Hu, Jianjun,Zhang, Wanqi,Chauhan, Surinder Singh,Shi, Changqing,Song, Yumeng,Zhao, Yubing,Wang, Zhehong,Cheng, Long,Zhang, Yingyu The Korean Society of Veterinary Science 2020 Journal of Veterinary Science Vol.21 No.2

Background: Bovine papilloma is a neoplastic disease caused by bovine papillomaviruses (BPVs), which were recently divided into 5 genera and at least 24 genotypes. Objectives: The complete genome sequence of BPV type 15 (BPV Aks-02), a novel putative BPV type from skin samples from infected cows in Southern Xinjiang China, was determined by collecting warty lesions, followed by DNA extraction and amplicon sequencing. Methods: DNA was analyzed initially by polymerase chain reaction (PCR) using the degenerate primers FAP59 and FAP64. The complete genome sequences of the BPV Aks-02 were amplified by PCR using the amplification primers and sequencing primers. Sequence analysis and phylogenetic analysis were performed using bio-informatic software. Results: The nucleotide sequence of the L1 open reading frame (ORF) of BPV Aks-02 was 75% identity to the L1 ORF of BPV-9 reference strain from GenBank. The complete genome consisted of 7,189 base pairs (G + C content of 42.50%) that encoded 5 early (E8, E7, E1, E2, and E4) and 2 late (L1 and L2) genes. The E7 protein contained a consensus CX₂CX₂₉CX₂C zinc-binding domain and a LxCxE motif. Among the different members of this group, the percentages of the complete genome and ORFs (including 5 early and 2 late ORFs) sequence identity of BPV Aks-02 were closer to the genus Xipapillomavirus 1 of the Xipapillomavirus genus. Phylogenetic analysis and sequence similarities based on the L1 ORF of BPV Aks-02 revealed the same cluster. Conclusions: The results suggest that BPV type (BPV Aks-02) clustered with members of the Xipapillomavirus genus as BPV 15 and were closely related to Xipapillomavirus 1.

Combined Application Effects of Arbuscular Mycorrhizal Fungi and Biochar on the Rhizosphere Fungal Community of Allium fistulosum L.

Ji Chunxiang,Li Yingyue,Xiao Qingchen,Li Zishan,Wang Boyan,Geng Xiaowan,Lin Keqing,Zhang Qing,Jin Yuan,Zhai Yuqian,Li Xiaoyu,Chen Jin 한국미생물·생명공학회 2023 Journal of microbiology and biotechnology Vol.33 No.8

Arbuscular mycorrhizal fungi (AMF) are widespread soil endophytic fungi, forming mutualistic relationships with the vast majority of land plants. Biochar (BC) has been reported to improve soil fertility and promote plant growth. However, limited studies are available concerning the combined effects of AMF and BC on soil community structure and plant growth. In this work, a pot experiment was designed to investigate the effects of AMF and BC on the rhizosphere microbial community of Allium fistulosum L. Using Illumina high-throughput sequencing, we showed that inoculation of AMF and BC had a significant impact on soil microbial community composition, diversity, and versatility. Increases were observed in both plant growth (the plant height by 8.6%, shoot fresh weight by 12.1%) and root morphological traits (average diameter by 20.5%). The phylogenetic tree also showed differences in the fungal community composition in A. fistulosum. In addition, Linear discriminant analysis (LDA) effect size (LEfSe) analysis revealed that 16 biomarkers were detected in the control (CK) and AMF treatment, while only 3 were detected in the AMF + BC treatment. Molecular ecological network analysis showed that the AMF + BC treatment group had a more complex network of fungal communities, as evidenced by higher average connectivity. The functional composition spectrum showed significant differences in the functional distribution of soil microbial communities among different fungal genera. The structural equation model (SEM) confirmed that AMF could improve the microbial multifunctionality by regulating the rhizosphere fungal diversity and soil properties. Our findings provide new information on the effects of AMF and biochar on plants and soil microbial communities.

Complete genome and phylogenetic analysis of bovine papillomavirus type 15 in Southern Xinjiang dairy cow

Jianjun Hu,Wanqi Zhang,Surinder Singh Chauhan,Changqing Shi,Yumeng Song,Yubing Zhao,Zhehong Wang,Long Cheng,Yingyu Zhang 대한수의학회 2020 Journal of Veterinary Science Vol.21 No.6

Background: Bovine papilloma is a neoplastic disease caused by bovine papillomaviruses (BPVs), which were recently divided into 5 genera and at least 24 genotypes. Objectives: The complete genome sequence of BPV type 15 (BPV Aks-02), a novel putative BPV type from skin samples from infected cows in Southern Xinjiang China, was determined by collecting warty lesions, followed by DNA extraction and amplicon sequencing. Methods: DNA was analyzed initially by polymerase chain reaction (PCR) using the degenerate primers FAP59 and FAP64. The complete genome sequences of the BPV Aks-02 were amplified by PCR using the amplification primers and sequencing primers. Sequence analysis and phylogenetic analysis were performed using bio-informatic software. Results: The nucleotide sequence of the L1 open reading frame (ORF) of BPV Aks-02 was 75% identity to the L1 ORF of BPV-9 reference strain from GenBank. The complete genome consisted of 7,189 base pairs (G + C content of 42.50%) that encoded 5 early (E8, E7, E1, E2, and E4) and 2 late (L1 and L2) genes. The E7 protein contained a consensus CX2CX29CX2C zinc-binding domain and a LxCxE motif. Among the different members of this group, the percentages of the complete genome and ORFs (including 5 early and 2 late ORFs) sequence identity of BPV Aks-02 were closer to the genus Xipapillomavirus 1 of the Xipapillomavirus genus. Phylogenetic analysis and sequence similarities based on the L1 ORF of BPV Aks-02 revealed the same cluster. Conclusions: The results suggest that BPV type (BPV Aks-02) clustered with members of the Xipapillomavirus genus as BPV 15 and were closely related to Xipapillomavirus 1.

에코레이블이 부착된 세제의 구매의도와 구매경험에 따른 소비자유형 및 관련요인 분석

유소이(So-Ye YOU),장영유(Yingyu ZHANG) 전북대학교 산업경제연구소 2012 아태경상저널 Vol.4 No.3

본 연구는 에코레이블이 부착된 세제를 대상으로 소비자들의 구매의도와 구매경험 간의 관련성을 파악하고, 소비자를 4가지 유형으로 분류(구매경험과 구매의도 모두 없음, 구매의도는 있으나 경험은 없음, 경험은 있으나 의도는 없음, 의도와 경험 모두 있음)하였다. 분류된 소비자유형 간 특성들의 차이검증과 더불어 의도와 경험이 모두 없는 유형과 비교하여 각각 의도만 있는 유형, 경험만 있는 유형 및 의도와 경험 모두 있는 유형의 행동차이에 영향을 주는 요인을 분석하였다. 분석 결과 에코레이블이 부착된 세제의 구매의도와 구매경험은 유의하게 상관성이 있는 것으로 나타났다. 제품 특성들에 있어서는 에코레이블 이해, 선택자신감, 준거집단 영향력, 광고효과, 제품관심, 가격가치지각 및 정부정책(보조금, 세금)에 대한 소비자 선호 등이 소비자유형별로 유의한 차이가 있는 것으로 나타났다. 관련요인의 경우 의도와 경험이 모두 없는 유형과 비교하여 다른 모든 유형들에서 연령은 유의한 것으로 나타났으며, 교육수준은 의도와 경험 모두를 가진 소비자유형에서 유의한 것으로 나타났다. 또한 광고효과는 의도만 있거나 경험과 의도 모두 있는 소비자들의 경우 유의한 것으로 나타났으며, 제품에 대한 관심과 환경표시에 대한 이해는 경험이 있는 소비자만이 유의한 것으로 나타났다. 이 밖에 제품선택에 대한 자신감은 경험이 있는 소비자의 경우 부적으로 유의한 것으로 나타났으나, 경험과 의도 모두 있는 소비자의 경우 정적으로 유의한 것으로 나타나 유형별로 상이한 효과가 있음을 알 수 있다. 본 연구는 환경친화적 소비행동을 파악하기 위하여 기본적으로 고려하는 인구통계 및 사회경제적 변수 외에 환경에 대한 관심이나 지식 등의 영향력을 파악함으로써 소비행동을 보다 환경친화적으로 변화시킬 수 있는 방안을 모색하는데 유용한 정보가 될 것이다. The purposes of this study were to explore the relationship between purchase intention and purchase experience for eco-labelled detergent and to test mean differences(ANOVA analysis) among four types(no intention and no experience, intention but no experience, no intention but experience, both intention and experience) categorized by both intention and experience and to test the influencing factors for three other types compared with the consumer type of no intention and no purchase(multinomial logit analysis). As the result, purchase intention and experience of eco-labeled detergent were significantly correlated each other. Then four types of consumers were found to respond significantly to be different for most variables such as understanding eco-label, self-confidence of choice, reference group, advertisement, product concern, price perception, policies beside sex, education and income. In addition, age was found to significantly influence to all types of consumer, while education was to the type of consumer having both intention and experience. Also advertisement was found to be significantly influence to the type of consumer for intention alone and having both, while self-confidence of product choice was to influence for the type of experience only and having both, which have opposite directions. Finally, this study might provide useful information to consumers, related industries and policy makers by exploring the psychological factors such as values, knowledge, concern for environment with socio-demographics.