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Ji Chunxiang,Li Yingyue,Xiao Qingchen,Li Zishan,Wang Boyan,Geng Xiaowan,Lin Keqing,Zhang Qing,Jin Yuan,Zhai Yuqian,Li Xiaoyu,Chen Jin 한국미생물·생명공학회 2023 Journal of microbiology and biotechnology Vol.33 No.8
Arbuscular mycorrhizal fungi (AMF) are widespread soil endophytic fungi, forming mutualistic relationships with the vast majority of land plants. Biochar (BC) has been reported to improve soil fertility and promote plant growth. However, limited studies are available concerning the combined effects of AMF and BC on soil community structure and plant growth. In this work, a pot experiment was designed to investigate the effects of AMF and BC on the rhizosphere microbial community of Allium fistulosum L. Using Illumina high-throughput sequencing, we showed that inoculation of AMF and BC had a significant impact on soil microbial community composition, diversity, and versatility. Increases were observed in both plant growth (the plant height by 8.6%, shoot fresh weight by 12.1%) and root morphological traits (average diameter by 20.5%). The phylogenetic tree also showed differences in the fungal community composition in A. fistulosum. In addition, Linear discriminant analysis (LDA) effect size (LEfSe) analysis revealed that 16 biomarkers were detected in the control (CK) and AMF treatment, while only 3 were detected in the AMF + BC treatment. Molecular ecological network analysis showed that the AMF + BC treatment group had a more complex network of fungal communities, as evidenced by higher average connectivity. The functional composition spectrum showed significant differences in the functional distribution of soil microbial communities among different fungal genera. The structural equation model (SEM) confirmed that AMF could improve the microbial multifunctionality by regulating the rhizosphere fungal diversity and soil properties. Our findings provide new information on the effects of AMF and biochar on plants and soil microbial communities.
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Jingsong He,Ni Xie,Jianbo Yang,Hong Guan,Weicai Chen,Huisheng Wu,Zishan Yuan,Kun Wang,Guojin Li,Jie Sun,Limin Yu 한국유방암학회 2014 Journal of breast cancer Vol.17 No.3
Purpose: Synuclein-γ (SNCG), which was initially identified asbreast cancer specific gene 1, is highly expressed in advancedbreast cancers, but not in normal or benign breast tissue. Thisstudy aimed to evaluate the effects of SNCG siRNA-treatmenton breast cancer cells and elucidate the associated mechanisms. Methods: Vectors containing SNCG and negative control(NC) siRNAs were transfected into MDA-MB-231 cells; mRNAlevels were determined by real-time polymerase chain reaction. Cell proliferation was evaluated using the MTT assay, cell migrationwas assessed by the Transwell assay, apoptosis and cellcycle analyses were conducted with the flow cytometer, andWestern blot analysis was performed to determine the relativelevels of AKT, ERK, p-AKT, and p-ERK expression. Results:SNCG mRNA levels were significantly reduced in MDA-MB-231cells transfected with SNCG siRNA. Our results indicate that inSNCG siRNA-treated cells, cell migration and proliferation decreasedsignificantly, apoptosis was induced, and the cell cyclewas arrested. Western blot analysis indicated that the proteinlevels of p-AKT and p-ERK were much lower in the SNCG siRNA-treated groups, than in the control and NC groups. Conclusion:SNCG siRNA could decrease the migration and proliferationof breast cancer cells by downregulating the phosphorylationof AKT and ERK.
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