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Hongli Gong,Boyan Wang,Yi Shi,Yong Shi,Xiyan Xiao,Pengyu Cao,Lei Tao,Yuezhu Wang,Liang Zhou 한국미생물학회 2017 The journal of microbiology Vol.55 No.8
The pharynx is an important site of microbiota colonization, but the bacterial populations at this site have been relatively unexplored by culture-independent approaches. The aim of this study was to characterize the microbiota structure of the pharynx. Pyrosequencing of 16S rRNA gene libraries was used to characterize the pharyngeal microbiota using swab samples from 68 subjects with laryngeal cancer and 28 subjects with vocal cord polyps. Overall, the major phylum was Firmicutes, with Streptococcus as the predominant genus in the pharyngeal communities. Nine core operational taxonomic units detected from Streptococcus, Fusobacterium, Prevotella, Granulicatella, and Veillonella accounted for 21.3% of the total sequences detected. However, there was no difference in bacterial communities in the pharynx from patients with laryngeal cancer and vocal cord polyps. The relative abundance of Firmicutes was inversely correlated with Fusobacteria, Proteobacteria, Actinobacteria, and Bacteroidetes. The correlation was evident at the genus level, and the relative abundance of Streptococcus was inversely associated with Fusobacterium, Leptotrichia, Neisseria, Actinomyces, and Prevotella. This study presented a profile for the overall structure of the microbiota in pharyngeal swab samples. Inverse correlations were found between Streptococcus and other bacterial communities, suggesting that potential antagonism may exist among pharyngeal microbiota.
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Ji Chunxiang,Li Yingyue,Xiao Qingchen,Li Zishan,Wang Boyan,Geng Xiaowan,Lin Keqing,Zhang Qing,Jin Yuan,Zhai Yuqian,Li Xiaoyu,Chen Jin 한국미생물·생명공학회 2023 Journal of microbiology and biotechnology Vol.33 No.8
Arbuscular mycorrhizal fungi (AMF) are widespread soil endophytic fungi, forming mutualistic relationships with the vast majority of land plants. Biochar (BC) has been reported to improve soil fertility and promote plant growth. However, limited studies are available concerning the combined effects of AMF and BC on soil community structure and plant growth. In this work, a pot experiment was designed to investigate the effects of AMF and BC on the rhizosphere microbial community of Allium fistulosum L. Using Illumina high-throughput sequencing, we showed that inoculation of AMF and BC had a significant impact on soil microbial community composition, diversity, and versatility. Increases were observed in both plant growth (the plant height by 8.6%, shoot fresh weight by 12.1%) and root morphological traits (average diameter by 20.5%). The phylogenetic tree also showed differences in the fungal community composition in A. fistulosum. In addition, Linear discriminant analysis (LDA) effect size (LEfSe) analysis revealed that 16 biomarkers were detected in the control (CK) and AMF treatment, while only 3 were detected in the AMF + BC treatment. Molecular ecological network analysis showed that the AMF + BC treatment group had a more complex network of fungal communities, as evidenced by higher average connectivity. The functional composition spectrum showed significant differences in the functional distribution of soil microbial communities among different fungal genera. The structural equation model (SEM) confirmed that AMF could improve the microbial multifunctionality by regulating the rhizosphere fungal diversity and soil properties. Our findings provide new information on the effects of AMF and biochar on plants and soil microbial communities.
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Bo Yan,Xiuhe Wang,Yubo Yang 대한전기학회 2017 Journal of Electrical Engineering & Technology Vol.12 No.2
This paper presents an improved subdomain method to predict the magnet field distributions and electromagnetic performance of the surface-mounted permanent magnet (SPM) machines with static or dynamic eccentricity. Conventional subdomain models are either based on the scalar magnet potential to predict the rotor eccentricity effect or dependent on the magnetic vector potential without considering the eccentric rotor. In this paper, both the magnetic vector potential and the perturbation theory are introduced in order to accurately calculate the effect of rotor eccentricity on the open-circuit and armature reaction performance. The calculation results are presented and validated by the corresponding finite-element method (FEM) results.
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