Expression of Smoothened Protein in Colon Cancer and its Prognostic Value for Postoperative Liver Metastasis

Ding, Yin-Lu,Wang, Qi-San,Zhao, Wei-Min,Xiang, Lei Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.8

Backgrouds: The hedgehog (Hh) signaling pathway is composed of patched (PTCH) and smoothened (SMO), two transmembrane proteins, and downstream glioma-associated oncogene homolog (Gli) transcription factors. Hh signaling plays a pathological role in the occurrence and development of various cancers. Methods: To investigate the expression of SMO protein in colon cancer and its association with clinicopathological parameters and postoperative liver metastasis, immunohistochemistry was performed with paraffin-embedded specimens of 96 cases. Relationships between SMO protein expression and clinicopathological parameters, postoperative liver metastasis were analyzed. Results: IHC examination showed that SMO protein expression was significantly increased in colon cancer tissues compared to normal colon tissues (P = 0.042), positively related to lymph node metastases (P = 0.018) and higher T stages (P = 0.026). Postoperative live metastasis-free survival was significantly longer in the low SMO expression group than in those with high SMO expression ($48.7{\pm}8.02$ months vs $28.0{\pm}6.86$ months, P=0.036). Multivariate analysis showed SMO expression level to be an independent prognostic factor for postoperative live metastasis-free survival (95% confidence interval [CI] =1.46-2.82, P = 0.008). Conclusions: Our results suggest that in patients with colon cancer, the SMO expression level is an independent biomarker for postoperative liver metastasis, and SMO might play an important role in colon cancer progression.

ANALYSIS OF FORCE TRANSMISSION CHARACTERISTICS OF MODULAR DEFORMABLE TIRE

Kongshu Deng,Lu Zeng,Yicheng Ding,Zhurong Yin 한국자동차공학회 2020 International journal of automotive technology Vol.21 No.5

In order to improve the explosion-proof, puncture-proof and safety performance of tires, a new type of modular deformable tires which can be used in complex road conditions has been put forward. The main innovation is the absence of so-called inner tubes and the modular design of the tire — which mainly consist of six petal-shaped connecting blocks and wheel frames. Firstly, this paper analyzes the structure and working principle of the tire. On this basis, the mechanical modeling of the tire under driving and braking conditions has been established. At last, through ADAMS software, the dynamic simulation of this tire under four circumstances of driving, accelerating, decelerating and braking has been carried out, and the response characteristic curves have been obtained The simulation results show that the force transmission performance of the modular deformable tire is rather good under the above four conditions. The simulation results provide a theoretical foundation and guidance for the structural design of new modular deformable tires.

Equivalent Vertical Stiffness Design of Modular Deformable Wheel

Kongshu Deng,Yicheng Ding,Lu Zeng,Zhurong Yin,Hailiang Wu,Dilei Qian 한국자동차공학회 2021 International journal of automotive technology Vol.22 No.1

This study aims to determine the structural parameters of the equivalent vertical stiffness design of a modular deformable wheel. A gas stiffness model in the air cavity of the modular deformable wheel is established on the basis of the wheel’s structure and the ideal gas equation. Then, the change curve of the radial stiffness of the modular deformable wheel is obtained, and the design model of the equivalent vertical stiffness of the wheel is constructed on the basis of the gas stiffness model. Finally, some relevant design parameters of heavyweight and lightweight wheels are obtained through numerical calculation based on existing experimental data. The results show that the design parameters of the equivalent vertical stiffness of the modular deformable wheel can meet the ride comfort requirements of vehicles. The obtained wheel structure parameters and mechanical parameters will provide effective theoretical compliance for the selection of this type of wheel. Moreover, this modular deformable wheel not only meets the requirements of the riding comfort of pneumatic wheels, but also has the advantages that common pneumatic wheels do not have (such as explosion-proof and puncture-proof functions), which also provides the practical theoretical basis for the use and promotion of this wheel in the complex geological environment.

Tailoring physical and chemical microenvironments by polyether-amine in blended membranes for efficient CO2 separation

Xia Lv,Xueqin Li,Lu Huang,Siyuan Ding,Yin Lv,Jinli Zhang 한국화학공학회 2022 Korean Journal of Chemical Engineering Vol.39 No.3

Pebax® MH 1657 (Pebax)-based blend membranes with different polyether-amine (PEA) loadings were designed and fabricated for efficient CO2 separation. The CO2 separation performance of Pebax/PEA blend membranes was greatly improved in comparison with that of pure membranes. This was mainly because the introduced PEA tailored the physical and chemical microenvironments in blend membranes. Specifically, PEA was a liquid-like additive, which was beneficial to reduce the mass transfer resistance of gases and increase CO2 permeability. Meanwhile, PEA contained amino groups that acted as mobile carriers to tailor the chemical microenvironment in blend membranes. The mobile carriers preferentially reacted reversibly with CO2 molecules, facilitating CO2 transport in membranes. Compared with CO2/CH4 separation performance of pure Pebax membrane, CO2 permeability and CO2/CH4 separation factor of Pebax/PEA-3 increased by 144.8% and 29.4%, respectively. This study suggests that PEA is a promising membrane material for tailoring the physical and chemical microenvironments in blend membranes for efficient CO2 separation.

Force transmission performance for a novel deformation wheel with crank slider mechanism

Kongshu Deng,Hailiang Wu,Yicheng Ding,Lu Zeng,Zhurong Yin,Dilei Qian 대한기계학회 2020 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.34 No.6

This study aims to analyze the force transmission characteristics of a novel wheel composed of a mechanism of planar crank slider with virtual constraints. The principle of kinematic inversion is applied to analyze the force transmission characteristics of deformable wheel in an innovative way. First, the structural design of deformable wheel and diagram of mechanism motion are presented. Second, the model of force transmission characteristic of deformable wheel is established on the basis of principle of kinematic inversion. Third, the force changes in the two pairs of springs in the deformable wheel are investigated using MATLAB and ADAMS. Finally, the force transmission performance between the deformable and elastic wheel is compared. Results show that the possibility of a flat wheel can be avoided under severe geological conditions, and the force transmission performance is better than that of the traditional elastic wheel. The results of this study can provide theoretical guidance in designing a novel nonpneumatic wheel.

Radix et Rhizoma Ginseng chemoprevents both initiation and promotion of cutaneous carcinoma by enhancing cell-mediated immunity and maintaining redox homeostasis

Yu, Suyun,Wang, Siliang,Huang, Shuai,Wang, Wei,Wei, Zhonghong,Ding, Yushi,Wang, Aiyun,Huang, Shile,Chen, Wenxing,Lu, Yin The Korean Society of Ginseng 2020 Journal of Ginseng Research Vol.44 No.4

Background: Radix et Rhizoma Ginseng (thereafter called ginseng) has been used as a medicinal herb for thousands of years to maintain people's physical vitality and is also a non-organ-specific cancer preventive and therapeutic traditional medicine in several epidemiologic and preclinical studies. Owing to few toxic side effects and strong enhancement on body immunity, ginseng has admirable application potential and value in cancer chemoprevention. The study aims at investigating the chemopreventive effects of ginseng on cutaneous carcinoma and the underlying mechanisms. Methods: The mouse skin cancer model was induced by 7,12-dimethylbenz[a]anthracene/12-O-tetradecanoylphorbol-13-acetate. Ultraperformance liquid chromatography/mass spectrometry was used for identifying various ginsenosides, the main active ingredients of ginseng. Comprehensive approaches (including network pharmacology, bioinformatics, and experimental verification) were used to explore the potential targets of ginseng. Results: Ginseng treatment inhibited cutaneous carcinoma in terms of initiation and promotion. The content of Rb1, Rb2, Rc, and Rd ginsenosides was the highest in both mouse blood and skin tissues. Ginseng and its active components well maintained the redox homeostasis and modulated the immune response in the model. Specifically, ginseng treatment inhibited the initiation of skin cancer by enhancing T-cell-mediated immune response through upregulating HSP27 expression and inhibited the promotion of skin cancer by maintaining cellular redox homeostasis through promoting nuclear translocation of Nrf2. Conclusion: According to the study results, ginseng can be potentially used for cutaneous carcinoma as a chemopreventive agent by enhancing cell-mediated immunity and maintaining redox homeostasis with multiple components, targets, and links.

Radix et Rhizoma Ginseng chemoprevents both initiation and promotion of cutaneous carcinoma by enhancing cell-mediated immunity and maintaining redox homeostasis

Suyun Yu,Siliang Wang,Shuai Huang,Wei Wang,Zhonghong Wei,Yushi Ding,Aiyun Wang,Shile Huang,Wenxing Chen,Yin Lu 고려인삼학회 2020 Journal of Ginseng Research Vol.44 No.4

Background: Radix et Rhizoma Ginseng (thereafter called ginseng) has been used as a medicinal herb forthousands of years to maintain people’s physical vitality and is also a noneorgan-specific cancer preventiveand therapeutic traditional medicine in several epidemiologic and preclinical studies. Owing tofew toxic side effects and strong enhancement on body immunity, ginseng has admirable applicationpotential and value in cancer chemoprevention. The study aims at investigating the chemopreventiveeffects of ginseng on cutaneous carcinoma and the underlying mechanisms. Methods: The mouse skin cancer model was induced by 7,12-dimethylbenz[a]anthracene/12-O-tetradecanoylphorbol-13-acetate. Ultraperformance liquid chromatography/mass spectrometry was used foridentifying various ginsenosides, the main active ingredients of ginseng. Comprehensive approaches(including network pharmacology, bioinformatics, and experimental verification) were used to explorethe potential targets of ginseng. Results: Ginseng treatment inhibited cutaneous carcinoma in terms of initiation and promotion. Thecontent of Rb1, Rb2, Rc, and Rd ginsenosides was the highest in both mouse blood and skin tissues. Ginseng and its active components well maintained the redox homeostasis and modulated the immuneresponse in the model. Specifically, ginseng treatment inhibited the initiation of skin cancer byenhancing T-cellemediated immune response through upregulating HSP27 expression and inhibited thepromotion of skin cancer by maintaining cellular redox homeostasis through promoting nuclear translocationof Nrf2. Conclusion: According to the study results, ginseng can be potentially used for cutaneous carcinoma as achemopreventive agent by enhancing cell-mediated immunity and maintaining redox homeostasis withmultiple components, targets, and links.

Effects of Achyranthes Bidentata Polysaccharide on Growth Performance, Immunological, Adrenal, and Somatotropic Responses of Weaned Pigs Challenged with Escherichia coli Lipopolysaccharide

Guo, Guanglun,Liu, Yulan,Fan, Wei,Han, Jie,Hou, Yongqing,Yin, Yulong,Zhu, Huiling,Ding, Binying,Shi, Junxia,Lu, Jing,Wang, Huirong,Chao, Jin,Qu, Yonghua Asian Australasian Association of Animal Productio 2008 Animal Bioscience Vol.21 No.8

A study was conducted with 48 weaned barrows ($28{\pm}3d$, $8.45{\pm}0.14kg$) to determine the effect of Achyranthes bidentata polysaccharide (ABPS) supplementation on pig performance, immunological, adrenal and somatotropic responses following Escherichia coli lipopolysaccharide (LPS) challenge. The experiment was a $2{\times}2$ factorial design; the main factors included diet (supplementation with 0 or 500 mg/kg ABPS) and immunological challenge (LPS or saline). On d 14 and 21 of the trial, pigs were given an intraperitoneal injection with either $100{\mu}g/kg$ BW of LPS or an equivalent amount of sterile saline. Blood samples were obtained 3 h after injection for analysis of tumor necrosis factor-${\alpha}$ (TNF-${\alpha}$), prostaglandin $E_2$ ($PGE_2$), cortisol, growth hormone (GH), insulin-like growth factor (IGF)-I and immunoglobulin G (IgG). On d 2 after LPS challenge, peripheral blood lymphocyte proliferation (PBLP) was measured. LPS administration decreased average daily feed intake (ADFI) (p<0.05), had a tendency to decrease average daily gain (ADG) (p<0.10) during both the first and second challenge periods and increased (p<0.05) feed:gain ratio only during the first challenge period. ABPS tended to improve ADG (p<0.10) during the first challenge period, and improved ADG (p<0.05) and tended to improve ADFI (p<0.10) during the second challenge period. ABPS did not affect feed:gain ratio. An interaction (p<0.05) between LPS challenge and diet was observed for the plasma concentrations of TNF-${\alpha}$, $PGE_2$ and cortisol after both LPS challenges such that, among LPS-treated pigs, pigs fed the ABPS diet were lower for these indices than those receiving the control diet. In contrast, pigs fed the ABPS diet had higher IGF-I (p<0.05) compared with those fed the control diet. No effect of diet, LPS challenge or both on GH and IgG was observed after both LPS administrations. LPS challenge increased PBLP when these cells were incubated with $8{\mu}g/ml$ of LPS during both the challenge periods, and did likewise when incubated with $8{\mu}g/ml$ of concanavalin A only after the first challenge. ABPS had no effect on PBLP. These data demonstrate that ABPS alters the release of pro-inflammatory cytokines following an immunological challenge, which might enable pigs to achieve better performance.

Associations of hypoxia inducible factor-1a gene polymorphisms with susceptibility to digestive tract cancers: a case–control study and meta-analysis

Zhi-Hai Ni,Xian-Jun Liang,Jing-Gang Mo,Yi Zhang,Jian-Hua Liang,Yu-Sha Yang,Yong Zhou,Zhao-Hua Li,Jian-Liang Zhang,Yin-Lu Ding,Peng Zhang,Jin-Qing Wang 한국유전학회 2015 Genes & Genomics Vol.37 No.11

We aim to investigate the correlations of hypoxia inducible factor-1a (HIF-1a) C1772T (rs11549465) and G1790A (rs11549467) gene polymorphisms with digestive tract cancers. A sum of 267 digestive tract cancers patients were hospitalized in Taizhou Central Hospital of Zhejiang Province as case group between December 2012 and December 2014. Additionally, 275 healthy people who had a physical examination in our hospital at the same time were selected as control group. Polymerase chain reaction-restriction fragment length polymorphism was utilized for detecting allele and genotype frequency of different locus in case and control group. Meta-analysis was performed using Comprehensive Metaanalysis 2.0 (Biostat Inc., Englewood, New Jersey, USA). Our result showed statistical significance only exists in family history of cancer between case and control group (P\0.05). Both C1772T (rs11549465) and G1790A (rs11549467) polymorphisms showed positive correlations with an increasing risk of digestive tract cancers. The frequencies of TT genotype of C1772T (rs11549465) and GA, AA genotypes of G1790A (rs11549467) polymorphisms in case group were evidently higher compared with the controls (all P\0.05). Besides, the comparison of allele and dominant models of HIF-1a C1772T (rs11549465) and G1790A (rs11549467) between two groups showed a significant difference (all P\0.05). Meta-analysis results further confirmed that the onset risk of digestive tract cancers may be improved under allele and dominant models of HIF-1a C1772T (rs11549465) and G1790A (rs11549467) (all P\0.05). Single nucleotide polymorphisms of HIF-1a C1772T (rs11549465) and G1790A (rs11549467) may play a role in development of digestive tract cancers.