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      • KCI등재
      • KCI등재

        Expression profiles of genes involved in fatty acid and lipid biosynthesis in developing seeds of Paeonia ostii

        Li Chaoqiong,Hu Lizong,Que Beibei,Hu Yueran,Guo Yuanyuan,Zhang Minghui,Wang Zenan,Wang Xueqin,Liu Hongzhan,Wang Junsheng,Tian Huihui,Li Xiaoli 한국유전학회 2021 Genes & Genomics Vol.43 No.8

        Background Paeonia ostii seeds were identifed as novel sources of edible plant oil with a high proportion of α-linolenic acid, a type of n-3 fatty acid with many health benefts. Due to the unreliability of seed oil content and quality, it is necessary to discover the mechanism underlying lipid biosynthesis in Paeonia ostii seeds. Objectives This study aimed to identify the key genes involved in lipid biosynthesis in Paeonia ostii seeds by analyzing the relationship among the seed characteristics and the expression patterns of lipid genes in Paeonia ostii during seed development. Methods Preliminary research on Paeonia ostii seed development was carried out from 10 days after pollination until maturity, focusing on phenology, oil content and lipid profles. In addition, we investigated the spatiotemporal expression of 36 lipid biosynthetic genes in Paeonia ostii by using quantitative real-time PCR. Results The results suggested that the development of Paeonia ostii seeds from pollination to maturity could be divided into three periods. The 36 lipid genes showed various spatiotemporal expression patterns and fve gene groups with distinct temporal patterns during seed development were identifed by clustering analysis of expression data. Furthermore, the relationships between gene expression and lipid/fatty acid accumulation and some candidate key lipid genes were discussed. Conclusions This study provided the global patterns of fatty acid and lipid biosynthesis-related gene expression, which are critical to understanding the molecular basis of lipid biosynthesis and identifying the lipid accumulation rate-limiting genes during seed development.

      • KCI등재

        The Effects of Electric Field on Jet Behavior and Fiber Properties in Melt Electrospinning

        Xueqin Li,Yuansheng Zheng,Xiaoqi Mu,Binjie Xin,Lantian Lin 한국섬유공학회 2020 Fibers and polymers Vol.21 No.5

        The electric field and temperature are the two important factors that influence the diameter and properties of fiberin the melt electrospinning process. It is commonly known that the polymer jet behavior is governed by the electric fieldwithin spinning area. In present work, a comprehensively-designed and properly-conducted analysis was carried out toinvestigate into the effects of electric field on the jet behaviors, diameters, crystalline structure and mechanical properties ofthe resultant fibers. An auxiliary electrode was invited to enhance the electric field strength. The high-speed photography wasadopted to capture the jet motion, and also, the numerical simulation was used to understand the electric field distribution. Bymaking use of the whipping amplitude and whipping frequency, the characteristics of jet behavior were described. It wasfound that by applying an auxiliary electrode, the average fiber diameter reduced from 61.01 μm to 9.06 μm, and thecrystallinity and strength of the fiber was improved with the help of the higher electric filed intensity. In addition, the moreuniform electric field would produce finer and more uniform fiber because of the more stable jet motion.

      • KCI등재

        Direct-patterned copper/poly(ethylene oxide) composite electrodes for organic thin-film transistors through cone-jet mode by electrohydrodynamic jet printing

        Xinlin Li,Hyeok-jin Kwon,Xueqin Zhang,Ho Kwang Choi,Sooman Lim,Tae-Wook Kim,Se Hyun Kim 한국공업화학회 2020 Journal of Industrial and Engineering Chemistry Vol.85 No.-

        Direct-patterned Cu-based conductive electrodes were printed through electrohydodynamic (EHD) jetprinting via cone-jet mode. The introduction of a capping agent in the synthesis of the Cu ink limitedexcessive conductivity, which enabled the pristine Cu ink to achieve the various printing modes of EHDjet printing: dripping, micro-dripping, cone-jet, and multi-jet. We also obtained optimal printingconditions by adjusting the viscosity by adding poly(ethylene oxide) (PEO) to the pristine Cu inks,resulting in well-defined printing patterns. The optimized PEO content in the ink was determined tobe10 wt%, which gave us a stable cone-jet mode and well-defined Cu/PEO composite electrode lineswith sharp edges. We utilized the Cu/PEO composite electrode lines as source and drain and atriisopropylsilylethynyl (TIPS)–pentacene/PS blend as the active layer for bottom-gate bottom-contactorganicfield-effect transistors (OFETs). The resulting devices exhibited an averagefield-effect mobility(mFET), threshold voltage (Vth), and on/off current ratio (Ion/Ioff) of 0.253 cm2/V, 0.253 V, and106,respectively.

      • KCI등재

        Callus induction and plant regeneration from leaves of peony

        Xiangtao Zhu,Xueqin Li,Wenjie Ding,Songheng Jin,Yan Wang 한국원예학회 2018 Horticulture, Environment, and Biotechnology Vol.59 No.4

        Tree peony (Paeonia suffruticosa Andr.) is a valued ornamental plant. This study reports on peony callus induction, shoot organogenesis and plant regeneration using young peony leaves as explants. Various media containing diverse plant growth regulators were assessed for their potency in peony propagation. After exposure of dark-adapted leaf discs to 30 μmol m−2 s−1 of light, inoculation in Murashige and Skoog (MS) + 0.2 mg L−1 2,4-dichlorophenoxyacetic acid (2,4-D) + 0.2 mg L−1 a-naphthaleneacetic acid (NAA) + 3.0 mg L−1 thidiazuron (TDZ) medium resulted to the highest callus induction rate with values reaching up to 87.8%. We identified that MS + 0.2 mg L−1 NAA + 2.0 mg L−1 6-benzyladenine (6-BA) + 2.0 mg L−1 kinetin (KT), with a multiplication coefficient of 3.025, to be the optimal medium for further callus proliferation under light. Inoculation in MS + 2.0 mg L−16-BA + 0.2 mg L−1 NAA + 0.3 mg L−1 TDZ medium allowed 22.22% of callus cultures to differentiate into adventitious shoots, whereas a similar rate of root formation was detected when 1/2 MS + 0.1 mg L−1 NAA + 0.05 mg L−1 IBA + 30 g L−1 sucrose medium was used. Our findings provide important information on peony regeneration and present a new method for peony tissue culture that will potentially facilitate mass propagation and genetic engineering of peony plants.

      • KCI등재

        Hepatoprotective effect of phenylethanoid glycosides from Incarvillea compacta against CCl4-induced cytotoxicity in HepG2 cells

        Ting Shen,Xueqin Li,Weicheng Hu,Lijin Zhang,Xudong Xu,Haifeng Wu,Lilian Ji 한국응용생명화학회 2015 Applied Biological Chemistry (Appl Biol Chem) Vol.58 No.4

        The extraction and solvent partition of roots of Incarvillea compacta, a traditional Tibetan folk medicine, and repeated column chromatography and preparative high-performance liquid chromatography for n-butanol fraction yielded four phenylethanoid glycosides, crenatoside (1), 3000-O-methylcrenatoside (2), leucoseceptoside A (3), and martynoside (4). The chemical structures were identified on the basis of spectroscopic data analyses including NMR and MS. All compounds were isolated for the first time from the plant. Compound 1 exerted better 1,1-diphenyl-2-picrylhydrazyl free radical scavenging activity. In addition, compounds 1–4 were evaluated for their hepatoprotective activity on carbon tetrachloride (CCl4)- induced liver injury in HepG2 cells. Pretreatment of HepG2 cells with compound 1–4 significantly increased the viability on CCl4-induced cell death. Furthermore, compounds 1–4 also alleviated CCl4-induced hepatotoxicity by enhancement of the antioxidant enzyme activities of superoxide dismutase and reduction of the malondialdehyde content, intracellular ROS as well as NF-jB transactivation. Our results suggest that phenylethanoid glycosides ameliorate CCl4-induced cell injury, and this protection was likely due to antioxidative activity and down-regulation of NF-jB.

      • SCIEKCI등재

        Hepatoprotective effect of phenylethanoid glycosides from Incarvillea compacta against CCl<sub>4</sub>-induced cytotoxicity in HepG2 cells

        Shen, Ting,Li, Xueqin,Hu, Weicheng,Zhang, Lijin,Xu, Xudong,Wu, Haifeng,Ji, Lilian 한국응용생명화학회 2015 Applied Biological Chemistry (Appl Biol Chem) Vol.58 No.4

        The extraction and solvent partition of roots of Incarvillea compacta, a traditional Tibetan folk medicine, and repeated column chromatography and preparative high-performance liquid chromatography for n-butanol fraction yielded four phenylethanoid glycosides, crenatoside (1), 3'''-O-methylcrenatoside (2), leucoseceptoside A (3), and martynoside (4). The chemical structures were identified on the basis of spectroscopic data analyses including NMR and MS. All compounds were isolated for the first time from the plant. Compound 1 exerted better 1,1-diphenyl-2-picrylhydrazyl free radical scavenging activity. In addition, compounds 1-4 were evaluated for their hepatoprotective activity on carbon tetrachloride ($CCl_4$)-induced liver injury in HepG2 cells. Pretreatment of HepG2 cells with compound 1-4 significantly increased the viability on $CCl_4$-induced cell death. Furthermore, compounds 1-4 also alleviated $CCl_4$-induced hepatotoxicity by enhancement of the antioxidant enzyme activities of superoxide dismutase and reduction of the malondialdehyde content, intracellular ROS as well as NF-${\kappa}B$ transactivation. Our results suggest that phenylethanoid glycosides ameliorate $CCl_4$-induced cell injury, and this protection was likely due to antioxidative activity and down-regulation of NF-${\kappa}B$.

      • KCI등재

        Optimization of fructose dehydration to 5-hydroxymethylfurfural catalyzed by SO3H-bearing lignin-derived ordered mesoporous carbon

        Shuai Wang,Li Lyu,Guobao Sima, Ying Cui,Ying Cui,Baoxia Li,Xueqin Zhang,Linhuo Gan 한국화학공학회 2019 Korean Journal of Chemical Engineering Vol.36 No.7

        A sulfonated lignin-derived mesoporous carbon (LDMC-SO3H) was prepared from kraft lignin (KL) using phenolation and soft-template method followed by sulfonation. LDMC-SO3H bearing a sulfonic acid density of 0.65 mmol/g possessed a well-ordered 2D hexagonal mesoporous characteristics with mesopore volume of 0.067 cm3/g and specific surface area of 262m2/g as well as mesopore size of 3.42 nm. A high 5-hydroxymethylfurfural (5-HMF) yield of 98.0% with a full fructose conversion was obtained using LDMC-SO3H as catalyst under the optimized reaction conditions of reaction temperature and time of 140 oC and 120 min, initial fructose concentration of 100 g/L, catalyst load of 0.1mg/mg in DMSO. Furthermore, there was no obvious decrease in 5-HMF yield (95.0%) within the fivecycle experiment, highlighting the superior reusability and stability of LDMC-SO3H in fructose-to-5-HMF transformation.

      • KCI등재

        Tailoring physical and chemical microenvironments by polyether-amine in blended membranes for efficient CO2 separation

        Xia Lv,Xueqin Li,Lu Huang,Siyuan Ding,Yin Lv,Jinli Zhang 한국화학공학회 2022 Korean Journal of Chemical Engineering Vol.39 No.3

        Pebax® MH 1657 (Pebax)-based blend membranes with different polyether-amine (PEA) loadings were designed and fabricated for efficient CO2 separation. The CO2 separation performance of Pebax/PEA blend membranes was greatly improved in comparison with that of pure membranes. This was mainly because the introduced PEA tailored the physical and chemical microenvironments in blend membranes. Specifically, PEA was a liquid-like additive, which was beneficial to reduce the mass transfer resistance of gases and increase CO2 permeability. Meanwhile, PEA contained amino groups that acted as mobile carriers to tailor the chemical microenvironment in blend membranes. The mobile carriers preferentially reacted reversibly with CO2 molecules, facilitating CO2 transport in membranes. Compared with CO2/CH4 separation performance of pure Pebax membrane, CO2 permeability and CO2/CH4 separation factor of Pebax/PEA-3 increased by 144.8% and 29.4%, respectively. This study suggests that PEA is a promising membrane material for tailoring the physical and chemical microenvironments in blend membranes for efficient CO2 separation.

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